Novel human genes and gene expression products:II

ABSTRACT

This invention relates to novel human genes, to proteins expressed by the genes, and to variants of the proteins. The invention also relates to diagnostic assays and therapeutic agents related to the genes and proteins, including probes, antisense constructs, and antibodies. The subject nucleic acids have been found to be differentially regulated in tumor cells, particularly in colon cancer tissue.

RELATED APPLICATION INFORMATION

[0001] This application is based on Provisional Application Nos.60/117,393, filed Jan. 27, 1999, and 60/098,639, filed Aug. 31, 1998,which are incorporated herein by reference in their entirety. Thisapplication is a continuation-in-part of application Ser. No.09/328,111, filed on Jun. 8, 1999, which is incorporated by referenceherein, in its entirety.

FIELD OF THE INVENTION

[0002] The present invention provides nucleic acid sequences andproteins encoded thereby, as well as probes derived from the nucleicacid sequences, antibodies directed to the encoded proteins, anddiagnostic methods for detecting cancerous cells, especially coloncancer cells.

BACKGROUND OF THE INVENTION

[0003] Colorectal carcinoma is a malignant neoplastic disease. There isa high incidence of colorectal carcinoma in the Western world,particularly in the United States. Tumors of this type often metastasizethrough lymphatic and vascular channels. Many patients with colorectalcarcinoma eventually die from this disease. In fact, it is estimatedthat 62,000 persons in the United States alone die of colorectalcarcinoma annually.

[0004] However, if diagnosed early, colon cancer may be treatedeffectively by surgical removal of the cancerous tissue. Colorectalcancers originate in the colorectal epithelium and typically are notextensively vascularized (and therefore not invasive) during the earlystages of development. Colorectal cancer is thought to result from theclonal expansion of a single mutant cell in the epithelial lining of thecolon or rectum. The transition to a highly vascularized, invasive andultimately metastatic cancer which spreads throughout the body commonlytakes ten years or longer. If the cancer is detected prior to invasion,surgical removal of the cancerous tissue is an effective cure. However,colorectal cancer is often detected only upon manifestation of clinicalsymptoms, such as pain and black tarry stool. Generally, such symptomsare present only when the disease is well established, often aftermetastasis has occurred, and the prognosis for the patient is poor, evenafter surgical resection of the cancerous tissue. Early detection ofcolorectal cancer therefore is important in that detection maysignificantly reduce its morbidity.

[0005] Invasive diagnostic methods such as endoscopic examination allowfor direct visual identification, removal, and biopsy of potentiallycancerous growths such as polyps. Endoscopy is expensive, uncomfortable,inherently risky, and therefore not a practical tool for screeningpopulations to identify those with colorectal cancer. Non-invasiveanalysis of stool samples for characteristics indicative of the presenceof colorectal cancer or precancer is a preferred alternative for earlydiagnosis, but no known diagnostic method is available which reliablyachieves this goal. A reliable, non-invasive, and accurate technique fordiagnosing colon cancer at an early stage would help save many lives.

SUMMARY OF THE INVENTION

[0006] The present invention provides nucleic acid sequences andproteins encoded thereby, as well as probes derived from the nucleicacid sequences, antibodies directed to the encoded proteins, anddiagnostic methods for detecting cancerous cells, especially coloncancer cells. The sequences disclosed herein have been found to bedifferentially expressed in samples obtained from colon cancer celllines and/or colon cancer tissue. The 544 sequences that were obtainedwere analyzed by “blasting” the sequences against the publicly availabledatabases; based upon the Blast search results it was found that SEQ IDNos:1-35 contained novel sequences, SEQ ID Nos:36-168 contained ESTsequences and SEQ ID Nos:169-544 contained known sequences.

[0007] In one aspect, the invention provides an isolated nucleic acidcomprising a nucleotide sequence which hybridizes under stringentconditions to a sequence of SEQ ID Nos. 1-544 or a sequencecomplementary thereto. In a related embodiment, the nucleic acid is atleast about 80% or about 100% identical to a sequence corresponding toat least about 12, at least about 15, at least about 25, or at leastabout 40 consecutive nucleotides up to the full length of one of SEQ IDNos. 1-544 or a sequence complementary thereto or up to the full lengthof the gene of which said sequence is a fragment. In certainembodiments, a nucleic acid of the present invention includes at leastabout five, at least about ten, or at least about twenty nucleic acidsfrom a region designated as novel in Table 2. In certain otherembodiments, a nucleic acid of the present invention includes at leastabout five, at least about ten, or at least about twenty nucleotideswhich are not included in corresponding clones whose accession numbersare listed in Table 2.

[0008] In another aspect, the invention provides an isolated nucleicacid comprising a nucleotide sequence which hybridizes under stringentconditions to a sequence of SEQ ID Nos. 1-168, preferably SEQ ID Nos.1-35, or a sequence complementary thereto. In a related embodiment, thenucleic acid is at least about 80% or about 100% identical to a sequencecorresponding to at least about 12, at least about 15, at least about25, or at least about 40 consecutive nucleotides up to the full lengthof one of SEQ ID Nos. 1-168, preferably SEQ ID Nos. 1-35 or a sequencecomplementary thereto or up to the full length of the gene of which saidsequence is a fragment. In certain embodiments, a nucleic acid of thepresent invention includes at least about five, at least about ten, orat least about twenty nucleic acids from a region designated as novel inTable 2. In certain other embodiments, a nucleic acid of the presentinvention includes at least about five, at least about ten, or at leastabout twenty nucleotides which are not included in corresponding cloneswhose accession numbers are listed in Table 2.

[0009] In one embodiment, the invention provides a nucleic acidcomprising a nucleotide sequence which hybridizes under stringentconditions to a sequence of SEQ ID Nos. 1-1 68, preferably SEQ ID Nos.1-35, or a sequence complementary thereto, and a transcriptionalregulatory sequence operably linked to the nucleotide sequence to renderthe nucleotide sequence suitable for use as an expression vector. Inanother embodiment, the nucleic acid may be included in an expressionvector capable of replicating in a prokaryotic or eukaryotic cell. In arelated embodiment, the invention provides a host cell transfected withthe expression vector.

[0010] In another embodiment, the invention provides a transgenic animalhaving a transgene of a nucleic acid comprising a nucleotide sequencewhich hybridizes under stringent conditions to a sequence of SEQ ID Nos.1-168, preferably SEQ ID Nos 1-35, or a sequence complementary theretoincorporated in cells thereof. The transgene modifies the level ofexpression of the nucleic acid, the stability of a mRNA transcript ofthe nucleic acid, or the activity of the encoded product of the nucleicacid.

[0011] In yet another embodiment, the invention provides substantiallypure nucleic acid which hybridizes under stringent conditions to anucleic acid probe corresponding to at least about 12, at least about15, at least about 25, or at least about 40 consecutive nucleotides upto the full length of one of SEQ ID Nos. 1-168, preferably SEQ ID Nos1-35, or a sequence complementary thereto or up to the full length ofthe gene of which said sequence is a fragment. The invention alsoprovides an antisense oligonucleotide analog which hybridizes understringent conditions to at least 12, at least 25, or at least 50consecutive nucleotides of one of SEQ ID Nos. 1-544 up to the fulllength of one of SEQ ID Nos. 1-544 or a sequence complementary theretoor up to the full length of the gene of which said sequence is afragment, and which is resistant to cleavage by a nuclease, preferablyan endogenous endonuclease or exonuclease.

[0012] In another embodiment, the invention provides a probe/primercomprising a substantially purified oligonucleotide, saidoligonucleotide containing a region of nucleotide sequence whichhybridizes under stringent conditions to at least about 12, at leastabout 15, at least about 25, or at least about 40 consecutivenucleotides of sense or antisense sequence selected from SEQ ID Nos.1-168 up to the full length of one of SEQ ID Nos. 1-168 or a sequencecomplementary thereto or up to the full length of the gene of which saidsequence is a fragment. In preferred embodiments, the probe selectivelyhybridizes with a target nucleic acid. In another embodiment, the probemay include a label group attached thereto and able to be detected. Thelabel group may be selected from radioisotopes, fluorescent compounds,enzymes, and enzyme co-factors. The invention further provides arrays ofat least about 10, at least about 25, at least about 50, or at leastabout 100 different probes as described above attached to a solidsupport.

[0013] In yet another embodiment, the invention pertains to a method ofdetermining the phenotype of a cell, comprising detecting thedifferential expression, relative to a normal cell, of at least onenucleic acid which hybridizes under stringent conditions to one of SEQID Nos. 1-544, wherein the nucleic acid is differentially expressed byat least a factor of two, at least a factor of five, at least a factorof twenty, or at least a factor of fifty.

[0014] In another aspect, the invention provides polypeptides encoded bythe subject nucleic acids. In one embodiment, the invention pertains toa polypeptide including an amino acid sequence encoded by a nucleic acidcomprising a nucleotide sequence which hybridizes under stringentconditions to a sequence of SEQ ID Nos. 1-168 or a sequencecomplementary thereto, or a fragment comprising at least about 25, or atleast about 40 amino acids thereof. Further provided are antibodiesimmunoreactive with these polypeptides.

[0015] In still another aspect, the invention provides diagnosticmethods. In one embodiment, the invention pertains to a method fordetermining the phenotype of cells from a patient by providing a nucleicacid probe comprising a nucleotide sequence having at least 12, at leastabout 15, at least about 25, or at least about 40 consecutivenucleotides represented in a sequence of SEQ ID Nos. 1-544 up to thefull length of one of SEQ ID Nos. 1-544 or a sequence complementarythereto or up to the full length of the gene of which said sequence is afragment, obtaining a sample of cells from a patient, providing a secondsample of cells substantially all of which are non-cancerous, contactingthe nucleic acid probe under stringent conditions with mRNA of each ofsaid first and second cell samples, and comparing (a) the amount ofhybridization of the probe with mRNA of the first cell sample, with (b)the amount of hybridization of the probe with mRNA of the second cellsample, wherein a difference of at least a factor of two, at least afactor of five, at least a factor of twenty, or at least a factor offifty in the amount of hybridization with the mRNA of the first cellsample as compared to the amount of hybridization with the mRNA of thesecond cell sample is indicative of the phenotype of cells in the firstcell sample. Determining the phenotype includes determining thegenotype, as the term is used herein.

[0016] In another embodiment, the invention provides a test kit foridentifying an transformed cells, comprising a probe/primer as describedabove, for measuring a level of a nucleic acid which hybridizes understringent conditions to a nucleic acid of SEQ ID Nos. 1-544 in a sampleof cells isolated from a patient. In certain embodiments, the kit mayfurther include instructions for using the kit, solutions for suspendingor fixing the cells, detectable tags or labels, solutions for renderinga nucleic acid susceptible to hybridization, solutions for lysing cells,or solutions for the purification of nucleic acids.

[0017] In another embodiment, the invention provides a method ofdetermining the phenotype of a cell, comprising detecting thedifferential expression, relative to a normal cell, of at least oneprotein encoded by a nucleic acid which hybridizes under stringentconditions to one of SEQ ID Nos. 1-544, wherein the protein isdifferentially expressed by at least a factor of two, at least a factorof five, at least a factor of twenty, or at least a factor of fifty. Inone embodiment, the level of the protein is detected in an immunoassay.The invention also pertains to a method for determining the presence orabsence of a nucleic acid which hybridizes under stringent conditions toone of SEQ ID Nos. 1-168 in a cell, comprising contacting the cell witha probe as described above. The invention further provides a method fordetermining the presence or absence of a subject polypeptide encoded bya nucleic acid which hybridizes under stringent conditions to one of SEQID Nos. 1-168 in a cell, comprising contacting the cell with an antibodyas described above. In yet another embodiment, the invention provides amethod for determining the presence of an aberrant mutation (e.g.,deletion, insertion, or substitution of nucleic acids) or aberrantmethylation in a gene which hybridizes under stringent conditions to asequence of SEQ ID Nos. 1-168 or a sequence complementary thereto,comprising collecting a sample of cells from a patient, isolatingnucleic acid from the cells of the sample, contacting the nucleic acidsample with one or more primers which specifically hybridize to anucleic acid sequence of SEQ ID Nos. 1-544 under conditions such thathybridization and amplification of the nucleic acid occurs, andcomparing the presence, absence, or size of an amplification product tothe amplification product of a normal cell.

[0018] In one embodiment, the invention provides a test kit foridentifying transformed cells, comprising an antibody specific for aprotein encoded by a nucleic acid which hybridizes under stringentconditions to any one of SEQ Nos. 1-544. In certain embodiments, the kitfurther includes instructions for using the kit. In certain embodiments,the kit may further include instructions for using the kit, solutionsfor suspending or fixing the cells, detectable tags or labels, solutionsfor rendering a polypeptide susceptible to the binding of an antibody,solutions for lysing cells, or solutions for the purification ofpolypeptides.

[0019] In yet another aspect, the invention provides pharmaceuticalcompositions including the subject nucleic acids. In one embodiment, anagent which alters the level of expression in a cell of a nucleic acidwhich hybridizes under stringent conditions to one of SEQ ID Nos. 1-544or a sequence complementary thereto is identified by providing a cell,treating the cell with a test agent, determining the level of expressionin the cell of a nucleic acid which hybridizes under stringentconditions to one of SEQ ID Nos. 1-544 or a sequence complementarythereto, and comparing the level of expression of the nucleic acid inthe treated cell with the level of expression of the nucleic acid in anuntreated cell, wherein a change in the level of expression of thenucleic acid in the treated cell relative to the level of expression ofthe nucleic acid in the untreated cell is indicative of an agent whichalters the level of expression of the nucleic acid in a cell. Theinvention further provides a pharmaceutical composition comprising anagent identified by this method. In another embodiment, the inventionprovides a pharmaceutical composition which includes a polypeptideencoded by a nucleic acid having a nucleotide sequence that hybridizesunder stringent conditions to one of SEQ ID Nos. 1-544 or a sequencecomplementary thereto. In one embodiment, the invention pertains to apharmaceutical composition comprising a nucleic acid including asequence which hybridizes under stringent conditions to one of SEQ IDNos. 1-544 or a sequence complementary thereto.

BRIEF DESCRIPTION OF THE FIGURE BRIEF DESCRIPTION OF THE FIGUREexpression of gene products in cells. DETAILED DESCRIPTION OF THEINVENTION

[0020] The invention relates to nucleic acids having the disclosednucleotide sequences (SEQ ID Nos. 1-544), as well as full length cDNA,mRNA, and genes corresponding to these sequences, and to polypeptidesand proteins encoded by these nucleic acids and genes, and portionsthereof.

[0021] Also included are polypeptides and proteins encoded by thenucleic acids of SEQ ID Nos. 1-544. The various nucleic acids that canencode these polypeptides and proteins differ because of the degeneracyof the genetic code, in that most amino acids are encoded by more thanone triplet codon. The identity of such codons is well known in thisart, and this information can be used for the construction of thenucleic acids within the scope of the invention.

[0022] Nucleic acids encoding polypeptides and proteins that arevariants of the polypeptides and proteins encoded by the nucleic acidsand related cDNA and genes are also within the scope of the invention.The variants differ from wild-type protein in having one or more aminoacid substitutions that either enhance, add, or diminish a biologicalactivity of the wild-type protein. Once the amino acid change isselected, a nucleic acid encoding that variant is constructed accordingto the invention.

[0023] The following detailed description discloses how to obtain ormake full-length cDNA and human genes corresponding to the nucleicacids, how to express these nucleic acids and genes, how to identifystructural motifs of the genes, how to identify the function of aprotein encoded by a gene corresponding to an nucleic acid, how to usenucleic acids as probes in mapping and in tissue profiling, how to usethe corresponding polypeptides and proteins to raise antibodies, and howto use the nucleic acids, polypeptides, and proteins for therapeutic anddiagnostic purposes.

[0024] The sequences investigated herein have been found to bedifferentially expressed in samples obtained from colon cancer celllines and/or colon cancer tissue. However, it is also believed thatthese sequences may also have utility with other types of cancer. Inparticular, Table 3 provides nucleic acid sequences which areover-expressed in both cancer cell line SW 480 as well colon cancertissue obtained from various patients.

[0025] Accordingly, certain aspects of the present invention relate tonucleic acids differentially expressed in tumor tissue, especially coloncancer cell lines, polypeptides encoded by such nucleic acids, andantibodies immunoreactive with these polypeptides, and preparations ofsuch compositions. Moreover, the present invention provides diagnosticand therapeutic assays and reagents for detecting and treating disordersinvolving, for example, aberrant expression of the subject nucleicacids.

[0026] I. General

[0027] This invention relates in part to novel methods for identifyingand/or classifying cancerous cells present in a human tumors,particularly in solid tumors, e.g., carcinomas and sarcomas, such as,for example, breast or colon cancers. The method uses genes that aredifferentially expressed in cancer cell lines and/or cancer tissuecompared with related normal cells, such as normal colon cells, andthereby identifies or classifies tumor cells by the upregulation and/ordownregulation of expression of particular genes, an event which isimplicated in tumorigenesis.

[0028] Upregulation or increased expression of certain genes such asoncogenes, act to promote malignant growth. Downregulation or decreasedexpression of genes such as tumor suppressor genes also promotesmalignant growth. Thus, alteration in the expression of either type ofgene is a potential diagnostic indicator for determining whether asubject is at risk of developing or has cancer, e.g., colon cancer.

[0029] Accordingly, in one aspect, the invention also providesbiomarkers, such as nucleic acid markers, for human tumor cells, e.g.,for colon cancer cells. The invention also provides proteins encoded bythese nucleic acid markers.

[0030] The invention also features methods for identifying drugs usefulfor treatment of such cancer cells, and for treatment of a cancerouscondition, such as colon cancer. Unlike prior methods, the inventionprovides a means for identifying cancer cells at an early stage ofdevelopment, so that premalignant cells can be identified prior to theirspreading throughout the human body. This allows early detection ofpotentially cancerous conditions, and treatment of those cancerousconditions prior to spread of the cancerous cells throughout the body,or prior to development of an irreversible cancerous condition.

[0031] II. Definitions

[0032] For convenience, the meaning of certain terms and phrases used inthe specification, examples, and appended claims, are provided below.

[0033] The term “an aberrant expression”, as applied to a nucleic acidof the present invention, refers to level of expression of that nucleicacid which differs from the level of expression of that nucleic acid inhealthy tissue, or which differs from the activity of the polypeptidepresent in a healthy subject. An activity of a polypeptide can beaberrant because it is stronger than the activity of its nativecounterpart. Alternatively, an activity can be aberrant because it isweaker or absent relative to the activity of its native counterpart. Anaberrant activity can also be a change in the activity; for example, anaberrant polypeptide can interact with a different target peptide. Acell can have an aberrant expression level of a gene due tooverexpression or underexpression of that gene.

[0034] The term “agonist”, as used herein, is meant to refer to an agentthat mimics or upregulates (e.g., potentiates or supplements) thebioactivity of a protein. An agonist can be a wild-type protein orderivative thereof having at least one bioactivity of the wild-typeprotein. An agonist can also be a compound that upregulates expressionof a gene or which increases at least one bioactivity of a protein. Anagonist can also be a compound which increases the interaction of apolypeptide with another molecule, e.g., a target peptide or nucleicacid.

[0035] The term “allele”, which is used interchangeably herein with“allelic variant”, refers to alternative forms of a gene or portionsthereof. Alleles occupy the same locus or position on homologouschromosomes. When a subject has two identical alleles of a gene, thesubject is said to be homozygous for that gene or allele. When a subjecthas two different alleles of a gene, the subject is said to beheterozygous for the gene. Alleles of a specific gene can differ fromeach other in a single nucleotide, or several nucleotides, and caninclude substitutions, deletions, and/or insertions of nucleotides. Anallele of a gene can also be a form of a gene containing mutations.

[0036] The term “allelic variant of a polymorphic region of a gene”refers to a region of a gene having one of several nucleotide sequencesfound in that region of the gene in other individuals.

[0037] “Antagonist” as used herein is meant to refer to an agent thatdownregulates (e.g., suppresses or inhibits) at least one bioactivity ofa protein. An antagonist can be a compound which inhibits or decreasesthe interaction between a protein and another molecule, e.g., a targetpeptide or enzyme substrate. An antagonist can also be a compound thatdownregulates expression of a gene or which reduces the amount ofexpressed protein present.

[0038] The term “antibody” as used herein is intended to include wholeantibodies, e.g., of any isotype (IgG, IgA, IgM, IgE, etc.), andincludes fragments thereof which are also specifically reactive with avertebrate, e.g., mammalian, protein. Antibodies can be fragmented usingconventional techniques and the fragments screened for utility in thesame manner as described above for whole antibodies. Thus, the termincludes segments of proteolytically-cleaved or recombinantly-preparedportions of an antibody molecule that are capable of selectivelyreacting with a certain protein. Nonlimiting examples of suchproteolytic and/or recombinant fragments include Fab, F(ab′)2, Fab′, Fv,and single chain antibodies (scFv) containing a V[L] and/or V[H] domainjoined by a peptide linker. The scFv's may be covalently ornon-covalently linked to form antibodies having two or more bindingsites. The subject invention includes polyclonal, monoclonal, or otherpurified preparations of antibodies and recombinant antibodies.

[0039] The phenomenon of “apoptosis” is well known, and can be describedas a programmed death of cells. As is known, apoptosis is contrastedwith “necrosis”, a phenomenon when cells die as a result of being killedby a toxic material, or other external effect. Apoptosis involveschromatic condensation, membrane blebbing, and fragmentation of DNA, allof which are generally visible upon microscopic examination.

[0040] A disease, disorder, or condition “associated with” or“characterized by” an aberrant expression of a nucleic acid refers to adisease, disorder, or condition in a subject which is caused by,contributed to by, or causative of an aberrant level of expression of anucleic acid.

[0041] As used herein the term “bioactive fragment of a polypeptide”refers to a fragment of a full-length polypeptide, wherein the fragmentspecifically agonizes (mimics) or antagonizes (inhibits) the activity ofa wild-type polypeptide. The bioactive fragment preferably is a fragmentcapable of interacting with at least one other molecule, e.g., protein,small molecule, or DNA, which a full length protein can bind.

[0042] “Biological activity” or “bioactivity” or “activity” or“biological function”, which are used interchangeably, herein mean aneffector or antigenic function that is directly or indirectly performedby a polypeptide (whether in its native or denatured conformation), orby any subsequence thereof. Biological activities include binding topolypeptides, binding to other proteins or molecules, activity as a DNAbinding protein, as a transcription regulator, ability to bind damagedDNA, etc. A bioactivity can be modulated by directly affecting thesubject polypeptide. Alternatively, a bioactivity can be altered bymodulating the level of the polypeptide, such as by modulatingexpression of the corresponding gene.

[0043] The term “biomarker” refers a biological molecule, e.g., anucleic acid, peptide, hormone, etc., whose presence or concentrationcan be detected and correlated with a known condition, such as a diseasestate.

[0044] “Cells,” “host cells”, or “recombinant host cells” are terms usedinterchangeably herein. It is understood that such terms refer not onlyto the particular subject cell but to the progeny or potential progenyof such a cell. Because certain modifications may occur in succeedinggenerations due to either mutation or environmental influences, suchprogeny may not, in fact, be identical to the parent cell, but are stillincluded within the scope of the term as used herein.

[0045] A “chimeric polypeptide” or “fusion polypeptide” is a fusion of afirst amino acid sequence encoding one of the subject polypeptides witha second amino acid sequence defining a domain (e.g., polypeptideportion) foreign to and not substantially homologous with any domain ofthe subject polypeptide. A chimeric polypeptide may present a foreigndomain which is found (albeit in a different polypeptide) in an organismwhich also expresses the first polypeptide, or it may be an“interspecies,” “intergenic,” etc., fusion of polypeptide structuresexpressed by different kinds of organisms. In general, a fusionpolypeptide can be represented by the general formula(X)_(n)-(Y)_(m)-(Z)_(n), wherein Y represents a portion of the subjectpolypeptide, and X and Z are each independently absent or representamino acid sequences which are not related to the native sequence foundin an organism, or which are not found as a polypeptide chain contiguouswith the subject sequence, where m is an integer greater than or equalto one, and each occurrence of n is, independently, 0 or an integergreater than or equal to 1 (n and m are preferably no greater than 5 or10).

[0046] A “delivery complex” shall mean a targeting means (e.g., amolecule that results in higher affinity binding of a nucleic acid,protein, polypeptide or peptide to a target cell surface and/orincreased cellular or nuclear uptake by a target cell). Examples oftargeting means include: sterols (e.g., cholesterol), lipids (e.g., acationic lipid, virosome or liposome), viruses (e.g., adenovirus,adeno-associated virus, and retrovirus), or target cell-specific bindingagents (e.g., ligands recognized by target cell specific receptors).Preferred complexes are sufficiently stable in vivo to preventsignificant uncoupling prior to internalization by the target cell.However, the complex is cleavable under appropriate conditions withinthe cell so that the nucleic acid, protein, polypeptide or peptide isreleased in a functional form.

[0047] As is well known, genes or a particular polypeptide may exist insingle or multiple copies within the genome of an individual. Suchduplicate genes may be identical or may have certain modifications,including nucleotide substitutions, additions or deletions, which allstill code for polypeptides having substantially the same activity. Theterm “DNA sequence encoding a polypeptide” may thus refer to one or moregenes within a particular individual. Moreover, certain differences innucleotide sequences may exist between individual organisms, which arecalled alleles. Such allelic differences may or may not result indifferences in amino acid sequence of the encoded polypeptide yet stillencode a polypeptide with the same biological activity.

[0048] The term “equivalent” is understood to include nucleotidesequences encoding functionally equivalent polypeptides. Equivalentnucleotide sequences will include sequences that differ by one or morenucleotide substitutions, additions or deletions, such as allelicvariants; and will, therefore, include sequences that differ from thenucleotide sequence of the nucleic acids shown in SEQ ID NOs:1-544 dueto the degeneracy of the genetic code.

[0049] As used herein, the terms “gene”, “recombinant gene”, and “geneconstruct” refer to a nucleic acid of the present invention associatedwith an open reading frame, including both exon and (optionally) intronsequences.

[0050] A “recombinant gene” refers to nucleic acid encoding apolypeptide and comprising exon sequences, though it may optionallyinclude intron sequences which are derived from, for example, a relatedor unrelated chromosomal gene. The term “intron” refers to a DNAsequence present in a given gene which is not translated into proteinand is generally found between exons.

[0051] The term “growth” or “growth state” of a cell refers to theproliferative state of a cell as well as to its differentiative state.Accordingly, the term refers to the phase of the cell cycle in which thecell is, e.g., G0, G1, G2, prophase, metaphase, or telophase, as well asto its state of differentiation, e.g., undifferentiated, partiallydifferentiated, or fully differentiated. Without wanting to be limited,differentiation of a cell is usually accompanied by a decrease in theproliferative rate of a cell.

[0052] “Homology” or “identity” or “similarity” refers to sequencesimilarity between two peptides or between two nucleic acid molecules,with identity being a more strict comparison. Homology and identity caneach be determined by comparing a position in each sequence which may bealigned for purposes of comparison. When a position in the comparedsequence is occupied by the same base or amino acid, then the moleculesare identical at that position. A degree of homology or similarity oridentity between nucleic acid sequences is a function of the number ofidentical or matching nucleotides at positions shared by the nucleicacid sequences. A degree of identity of amino acid sequences is afunction of the number of identical amino acids at positions shared bythe amino acid sequences. A degree of homology or similarity of aminoacid sequences is a function of the number of amino acids, i.e.,structurally related, at positions shared by the amino acid sequences.An “unrelated” or “non-homologous” sequence shares less than 40%identity, though preferably less than 25% identity, with one of thesequences of the present invention.

[0053] The term “percent identical” refers to sequence identity betweentwo amino acid sequences or between two nucleotide sequences. Identitycan each be determined by comparing a position in each sequence whichmay be aligned for purposes of comparison. When an equivalent positionin the compared sequences is occupied by the same base or amino acid,then the molecules are identical at that position; when the equivalentsite occupied by the same or a similar amino acid residue (e.g., similarin steric and/or electronic nature), then the molecules can be referredto as homologous (similar) at that position. Expression as a percentageof homology, similarity, or identity refers to a function of the numberof identical or similar amino acids at positions shared by the comparedsequences. Various alignment algorithms and/or programs may be used,including FASTA, BLAST, or ENTREZ. FASTA and BLAST are available as apart of the GCG sequence analysis package (University of Wisconsin,Madison, Wis.), and can be used with, e.g., default settings. ENTREZ isavailable through the National Center for Biotechnology Information,National Library of Medicine, National Institutes of Health, Bethesda,Md. In one embodiment, the percent identity of two sequences can bedetermined by the GCG program with a gap weight of 1, e.g., each aminoacid gap is weighted as if it were a single amino acid or nucleotidemismatch between the two sequences.

[0054] Other techniques for alignment are described in Methods inEnzymology, vol. 266: Computer Methods for Macromolecular SequenceAnalysis (1996), ed. Doolittle, Academic Press, Inc., a division ofHarcourt Brace & Co., San Diego, Calif., USA. Preferably, an alignmentprogram that permits gaps in the sequence is utilized to align thesequences. The Smith-Waterman is one type of algorithm that permits gapsin sequence alignments. See Meth. Mol. Biol. 70: 173-187 (1997). Also,the GAP program using the Needleman and Wunsch alignment method can beutilized to align sequences. An alternative search strategy uses MPSRCHsoftware, which runs on a MASPAR computer. MPSRCH uses a Smith-Watermanalgorithm to score sequences on a massively parallel computer. Thisapproach improves ability to pick up distantly related matches, and isespecially tolerant of small gaps and nucleotide sequence errors.Nucleic acid-encoded amino acid sequences can be used to search bothprotein and DNA databases.

[0055] Databases with individual sequences are described in Methods inEnzymology, ed. Doolittle, supra. Databases include Genbank, EMBL, andDNA Database of Japan (DDBJ).

[0056] Preferred nucleic acids have a sequence at least 70%, and morepreferably 80% identical and more preferably 90% and even morepreferably at least 95% identical to an nucleic acid sequence of asequence shown in one of SEQ ID NOS:1-544. Nucleic acids at least 90%,more preferably 95%, and most preferably at least about 98-99% identicalwith a nucleic sequence represented in one of SEQ ID NOS:1-544 are ofcourse also within the scope of the invention. In preferred embodiments,the nucleic acid is mammalian.

[0057] The term “interact” as used herein is meant to include detectableinteractions (e.g., biochemical interactions) between molecules, such asinteraction between protein-protein, protein-nucleic acid, nucleicacid-nucleic acid, and protein-small molecule or nucleic acid-smallmolecule in nature.

[0058] The term “isolated” as used herein with respect to nucleic acids,such as DNA or RNA, refers to molecules separated from other DNAs, orRNAs, respectively, that are present in the natural source of themacromolecule. The term isolated as used herein also refers to a nucleicacid or peptide that is substantially free of cellular material, viralmaterial, or culture medium when produced by recombinant DNA techniques,or chemical precursors or other chemicals when chemically synthesized.Moreover, an “isolated nucleic acid” is meant to include nucleic acidfragments which are not naturally occurring as fragments and would notbe found in the natural state. The term “isolated” is also used hereinto refer to polypeptides which are isolated from other cellular proteinsand is meant to encompass both purified and recombinant polypeptides.

[0059] The terms “modulated” and “differentially regulated” as usedherein refer to both upregulation (i.e., activation or stimulation(e.g., by agonizing or potentiating)) and downregulation (i.e.,inhibition or suppression (e.g., by antagonizing, decreasing orinhibiting)).

[0060] The term “mutated gene” refers to an allelic form of a gene,which is capable of altering the phenotype of a subject having themutated gene relative to a subject which does not have the mutated gene.If a subject must be homozygous for this mutation to have an alteredphenotype, the mutation is said to be recessive. If one copy of themutated gene is sufficient to alter the genotype of the subject, themutation is said to be dominant. If a subject has one copy of themutated gene and has a phenotype that is intermediate between that of ahomozygous and that of a heterozygous subject (for that gene), themutation is said to be co-dominant.

[0061] The designation “N”, where it appears in the accompanyingSequence Listing, indicates that the identity of the correspondingnucleotide is unknown. “N” should therefore not necessarily beinterpreted as permitting substitution with any nucleotide, e.g., A, T,C, or G, but rather as holding the place of a nucleotide whose identityhas not been conclusively determined.

[0062] The “non-human animals” of the invention include mammalians suchas rodents, non-human primates, sheep, dog, cow, chickens, amphibians,reptiles, etc. Preferred non-human animals are selected from the rodentfamily including rat and mouse, most preferably mouse, though transgenicamphibians, such as members of the Xenopus genus, and transgenicchickens can also provide important tools for understanding andidentifying agents which can affect, for example, embryogenesis andtissue formation. The term “chimeric animal” is used herein to refer toanimals in which the recombinant gene is found, or in which therecombinant gene is expressed in some but not all cells of the animal.The term “tissue-specific chimeric animal” indicates that one of therecombinant genes is present and/or expressed or disrupted in sometissues but not others.

[0063] As used herein, the term “nucleic acid” refers to polynucleotidessuch as deoxyribonucleic acid (DNA), and, where appropriate, ribonucleicacid (RNA). The term should also be understood to include, asequivalents, analogs of either RNA or DNA made from nucleotide analogs,and, as applicable to the embodiment being described, single (sense orantisense) and double-stranded polynucleotides. ESTs, chromosomes,cDNAs, mRNAs, and rRNAs are representative examples of molecules thatmay be referred to as nucleic acids.

[0064] The term “nucleotide sequence complementary to the nucleotidesequence of SEQ ID NO. x” refers to the nucleotide sequence of thecomplementary strand of a nucleic acid strand having SEQ ID NO. x. Theterm “complementary strand” is used herein interchangeably with the term“complement”. The complement of a nucleic acid strand can be thecomplement of a coding strand or the complement of a noncoding strand.

[0065] The term “polymorphism” refers to the coexistence of more thanone form of a gene or portion (e.g., allelic variant) thereof. A portionof a gene of which there are at least two different forms, i.e., twodifferent nucleotide sequences, is referred to as a “polymorphic regionof a gene”. A polymorphic region can be a single nucleotide, theidentity of which differs in different alleles. A polymorphic region canalso be several nucleotides long.

[0066] A “polymorphic gene” refers to a gene having at least onepolymorphic region.

[0067] As used herein, the term “promoter” means a DNA sequence thatregulates expression of a selected DNA sequence operably linked to thepromoter, and which effects expression of the selected DNA sequence incells. The term encompasses “tissue specific” promoters, i.e., promoterswhich effect expression of the selected DNA sequence only in specificcells (e.g., cells of a specific tissue). The term also covers so-called“leaky” promoters, which regulate expression of a selected DNA primarilyin one tissue, but cause expression in other tissues as well. The termalso encompasses non-tissue specific promoters and promoters thatconstitutively expressed or that are inducible (i.e., expression levelscan be controlled).

[0068] The terms “protein”, “polypeptide”, and “peptide” are usedinterchangeably herein when referring to a gene product.

[0069] The term “recombinant protein” refers to a polypeptide of thepresent invention which is produced by recombinant DNA techniques,wherein generally, DNA encoding a polypeptide is inserted into asuitable expression vector which is in turn used to transform a hostcell to produce the heterologous protein. Moreover, the phrase “derivedfrom”, with respect to a recombinant gene, is meant to include withinthe meaning of “recombinant protein” those proteins having an amino acidsequence of a native polypeptide, or an amino acid sequence similarthereto which is generated by mutations including substitutions anddeletions (including truncation) of a naturally occurring form of thepolypeptide.

[0070] “Small molecule” as used herein, is meant to refer to acomposition, which has a molecular weight of less than about 5 kD andmost preferably less than about 4 kD. Small molecules can be nucleicacids, peptides, polypeptides, peptidomimetics, carbohydrates, lipids orother organic (carbon-containing) or inorganic molecules. Manypharmaceutical companies have extensive libraries of chemical and/orbiological mixtures, often fungal, bacterial, or algal extracts, whichcan be screened with any of the assays of the invention to identifycompounds that modulate a bioactivity.

[0071] As used herein, the term “specifically hybridizes” or“specifically detects” refers to the ability of a nucleic acid moleculeof the invention to hybridize to at least a portion of, for example,approximately 6, 12, 15, 20, 30, 50, 100, 150, 200, 300, 350, 400, 500,750, or 1000 contiguous nucleotides of a nucleic acid designated in anyone of SEQ ID Nos:1-544, or a sequence complementary thereto, ornaturally occurring mutants thereof, such that it has less than 15%,preferably less than 10%, and more preferably less than 5% backgroundhybridization to a cellular nucleic acid (e.g., mRNA or genomic DNA)encoding a different protein. In preferred embodiments, theoligonucleotide probe detects only a specific nucleic acid, e.g., itdoes not substantially hybridize to similar or related nucleic acids, orcomplements thereof.

[0072] “Transcriptional regulatory sequence” is a generic term usedthroughout the specification to refer to DNA sequences, such asinitiation signals, enhancers, and promoters, which induce or controltranscription of protein coding sequences with which they are operablylinked. In preferred embodiments, transcription of one of the genes isunder the control of a promoter sequence (or other transcriptionalregulatory sequence) which controls the expression of the recombinantgene in a cell-type in which expression is intended. It will also beunderstood that the recombinant gene can be under the control oftranscriptional regulatory sequences which are the same or which aredifferent from those sequences which control transcription of thenaturally-occurring forms of the polypeptide.

[0073] As used herein, the term “transfection” means the introduction ofa nucleic acid, e.g., via an expression vector, into a recipient cell bynucleic acid-mediated gene transfer. “Transformation”, as used herein,refers to a process in which a cell's genotype is changed as a result ofthe cellular uptake of exogenous DNA or RNA, and, for example, thetransformed cell expresses a recombinant form of a polypeptide or, inthe case of anti-sense expression from the transferred gene, theexpression of the target gene is disrupted.

[0074] As used herein, the term “transgene” means a nucleic acidsequence (or an antisense transcript thereto) which has been introducedinto a cell. A transgene could be partly or entirely heterologous, i.e.,foreign, to the transgenic animal or cell into which it is introduced,or, is homologous to an endogenous gene of the transgenic animal or cellinto which it is introduced, but which is designed to be inserted, or isinserted, into the animal's genome in such a way as to alter the genomeof the cell into which it is inserted (e.g., it is inserted at alocation which differs from that of the natural gene or its insertionresults in a knockout). A transgene can also be present in a cell in theform of an episome. A transgene can include one or more transcriptionalregulatory sequences and any other nucleic acid, such as introns, thatmay be necessary for optimal expression of a selected nucleic acid.

[0075] A “transgenic animal” refers to any animal, preferably anon-human mammal, bird or an amphibian, in which one or more of thecells of the animal contain heterologous nucleic acid introduced by wayof human intervention, such as by transgenic techniques well known inthe art. The nucleic acid is introduced into the cell, directly orindirectly by introduction into a precursor of the cell, by way ofdeliberate genetic manipulation, such as by microinjection or byinfection with a recombinant virus. The term genetic manipulation doesnot include classical cross-breeding, or in vitro fertilization, butrather is directed to the introduction of a recombinant DNA molecule.This molecule may be integrated within a chromosome, or it may beextra-chromosomally replicating DNA. In the typical transgenic animalsdescribed herein, the transgene causes cells to express a recombinantform of one of the subject polypeptide, e.g. either agonistic orantagonistic forms. However, transgenic animals in which the recombinantgene is silent are also contemplated, as for example, the FLP or CRErecombinase dependent constructs described below. Moreover, “transgenicanimal” also includes those recombinant animals in which gene disruptionof one or more genes is caused by human intervention, including bothrecombination and antisense techniques.

[0076] The term “treating” as used herein is intended to encompasscuring as well as ameliorating at least one symptom of the condition ordisease.

[0077] The term “vector” refers to a nucleic acid molecule capable oftransporting another nucleic acid to which it has been linked. One typeof preferred vector is an episome, i.e., a nucleic acid capable ofextra-chromosomal replication. Preferred vectors are those capable ofautonomous replication and/or expression of nucleic acids to which theyare linked. Vectors capable of directing the expression of genes towhich they are operatively linked are referred to herein as “expressionvectors”. In general, expression vectors of utility in recombinant DNAtechniques are often in the form of “plasmids” which refer generally tocircular double stranded DNA loops which, in their vector form are notbound to the chromosome. In the present specification, “plasmid” and“vector” are used interchangeably as the plasmid is the most commonlyused form of vector. However, the invention is intended to include suchother forms of expression vectors which serve equivalent functions andwhich become known in the art subsequently hereto.

[0078] The term “wild-type allele” refers to an allele of a gene which,when present in two copies in a subject results in a wild-typephenotype. There can be several different wild-type alleles of aspecific gene, since certain nucleotide changes in a gene may not affectthe phenotype of a subject having two copies of the gene with thenucleotide changes.

[0079] III. Nucleic Acids of the Present Invention

[0080] As described below, one aspect of the invention pertains toisolated nucleic acids, variants, and/or equivalents of such nucleicacids.

[0081] Nucleic acids of the present invention have been identified asdifferentially expressed in tumor cells, e.g., colon cancer-derived celllines (relative to the expression levels in normal tissue, e.g., normalcolon tissue and/or normal non-colon tissue), such as SEQ ID Nos. 1-544,preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, ora sequence complementary thereto. In certain embodiments, the subjectnucleic acids are differentially expressed by at least a factor of two,preferably at least a factor of five, even more preferably at least afactor of twenty, still more preferably at least a factor of fifty.Preferred nucleic acids include sequences identified as differentiallyexpressed both in colon cancer cell tissue and colon cancer cell lines.In preferred embodiments, nucleic acids of the present invention areupregulated in tumor cells, especially colon cancer tissue and/or coloncancer-derived cell lines. In another embodiment, nucleic acids of thepresent invention are downregulated in tumor cells, especially coloncancer tissue and/or colon cancer-derived cell lines.

[0082] Table 1 indicates those sequences which are over- orunderexpressed in a colon cancer-derived cell line relative to normaltissue, and further designates those sequences which are alsodifferentially regulated in colon cancer tissue. The designation Oindicates that the corresponding sequence was overexpressed, M indicatespossible overexpression, N indicates no differential expression, and Uindicates underexpression.

[0083] Genes which are upregulated, such as oncogenes, or downregulated,such as tumor suppressors, in aberrantly proliferating cells may betargets for diagnostic or therapeutic techniques. For example,upregulation of the cdc2 gene induces mitosis. Overexpression of themyt1 gene, a mitotic deactivator, negatively regulates the activity ofcdc2. Aberrant proliferation may thus be induced either by upregulatingcdc2 or by downregulating myt1. Similarly, downregulation of tumorsuppressors such as p53 and Rb have been implicated in tumorigenesis.

[0084] Particularly preferred polypeptides are those that are encoded bynucleic acid sequences at least about 70%, 75%, 80%, 90%, 95%, 97%, or98% similar to a nucleic acid sequence of SEQ ID Nos. 1-544. Preferably,the nucleic acid includes all or a portion (e.g., at least about 12, atleast about 15, at least about 25, or at least about 40 nucleotides) ofthe nucleotide sequence corresponding to the nucleic acid of SEQ ID Nos.1-168, preferably SEQ ID Nos. 1-35, or a sequence complementary thereto.

[0085] Still other preferred nucleic acids of the present inventionencode a polypeptide comprising at least a portion of a polypeptideencoded by one of SEQ ID Nos. 1-544. For example, preferred nucleic acidmolecules for use as probes/primers or antisense molecules (i.e.,noncoding nucleic acid molecules) can comprise at least about 12, 20,30, 50, 60, 70, 80, 90, or 100 base pairs in length up to the length ofthe complete gene. Coding nucleic acid molecules can comprise, forexample, from about 50, 60, 70, 80, 90, or 100 base pairs up to thelength of the complete gene.

[0086] Another aspect of the invention provides a nucleic acid whichhybridizes under low, medium, or high stringency conditions to a nucleicacid sequence represented by one of SEQ ID Nos. 1-168, preferably SEQ IDNos. 1-35, or a sequence complementary thereto. Appropriate stringencyconditions which promote DNA hybridization, for example, 6.0×sodiumchloride/sodium citrate (SSC) at about 45° C., followed by a wash of2.0×SSC at 50° C., are known to those skilled in the art or can be foundin Current Protocols in Molecular Biology, John Wiley & Sons, N.Y.(1989), 6.3.1-12.3.6. For example, the salt concentration in the washstep can be selected from a low stringency of about 2.0×SSC at 50° C. toa high stringency of about 0.2×SSC at 50° C. In addition, thetemperature in the wash step can be increased from low stringencyconditions at room temperature, about 22° C., to high stringencyconditions at about 65° C. Both temperature and salt may be varied, ortemperature or salt concentration may be held constant while the othervariable is changed. In a preferred embodiment, a nucleic acid of thepresent invention will bind to one of SEQ ID Nos. 1-168, preferably SEQID Nos. 1-35, or a sequence complementary thereto, under moderatelystringent conditions, for example at about 2.0×SSC and about 40° C. In aparticularly preferred embodiment, a nucleic acid of the presentinvention will bind to one of SEQ ID Nos. 1-168, preferably SEQ ID Nos.1-35, or a sequence complementary thereto, under high stringencyconditions.

[0087] In one embodiment, the invention provides nucleic acids whichhybridize under low stringency conditions of 6×SSC at room temperaturefollowed by a wash at 2×SSC at room temperature.

[0088] In another embodiment, the invention provides nucleic acids whichhybridize under high stringency conditions of 2×SSC at 65° C. followedby a wash at 0.2×SSC at 65° C.

[0089] Nucleic acids having a sequence that differs from the nucleotidesequences shown in one of SEQ ID Nos. 1-168, preferably SEQ ID Nos.1-35, or a sequence complementary thereto, due to degeneracy in thegenetic code, are also within the scope of the invention. Such nucleicacids encode functionally equivalent peptides (i.e., a peptide havingequivalent or similar biological activity) but differ in sequence fromthe sequence shown in the sequence listing due to degeneracy in thegenetic code. For example, a number of amino acids are designated bymore than one triplet. Codons that specify the same amino acid, orsynonyms (for example, CAU and CAC each encode histidine) may result in“silent” mutations which do not affect the amino acid sequence of apolypeptide. However, it is expected that DNA sequence polymorphismsthat do lead to changes in the amino acid sequences of the subjectpolypeptides will exist among mammals. One skilled in the art willappreciate that these variations in one or more nucleotides (e.g., up toabout 3-5% of the nucleotides) of the nucleic acids encodingpolypeptides having an activity of a polypeptide may exist amongindividuals of a given species due to natural allelic variation.

[0090] Also within the scope of the invention are nucleic acids encodingsplicing variants of proteins encoded by a nucleic acid of SEQ ID Nos.1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos.1-35, or a sequence complementary thereto, or natural homologs of suchproteins. Such homologs can be cloned by hybridization or PCR, asfurther described herein.

[0091] The polynucleotide sequence may also encode for a leadersequence, e.g., the natural leader sequence or a heterologous leadersequence, for a subject polypeptide. For example, the desired DNAsequence may be fused in the same reading frame to a DNA sequence whichaids in expression and secretion of the polypeptide from the host cell,for example, a leader sequence which functions as a secretory sequencefor controlling transport of the polypeptide from the cell. The proteinhaving a leader sequence is a preprotein and may have the leadersequence cleaved by the host cell to form the mature form of theprotein.

[0092] The polynucleotide of the present invention may also be fused inframe to a marker sequence, also referred to herein as “Tag sequence”encoding a “Tag peptide”, which allows for marking and/or purificationof the polypeptide of the present invention. In a preferred embodiment,the marker sequence is a hexahistidine tag, e.g., supplied by a PQE-9vector. Numerous other Tag peptides are available commercially. Otherfrequently used Tags include myc-epitopes (e.g., see Ellison et al.(1991) J Biol Chem 266:21150-21157) which includes a 10-residue sequencefrom c-myc, the pFLAG system (International Biotechnologies, Inc.), thepEZZ-protein A system (Pharmacia, N.J.), and a 16 amino acid portion ofthe Haemophilus influenza hemagglutinin protein. Furthermore, anypolypeptide can be used as a Tag so long as a reagent, e.g., an antibodyinteracting specifically with the Tag polypeptide is available or can beprepared or identified.

[0093] As indicated by the examples set out below, nucleic acids can beobtained from mRNA present in any of a number of eukaryotic cells, e.g.,and are preferably obtained from metazoan cells, more preferably fromvertebrate cells, and even more preferably from mammalian cells. Itshould also be possible to obtain nucleic acids of the present inventionfrom genomic DNA from both adults and embryos. For example, a gene canbe cloned from either a cDNA or a genomic library in accordance withprotocols generally known to persons skilled in the art. cDNA can beobtained by isolating total mRNA from a cell, e.g., a vertebrate cell, amammalian cell, or a human cell, including embryonic cells. Doublestranded cDNAs can then be prepared from the total mRNA, andsubsequently inserted into a suitable plasmid or bacteriophage vectorusing any one of a number of known techniques. The gene can also becloned using established polymerase chain reaction techniques inaccordance with the nucleotide sequence information provided by theinvention.

[0094] In certain embodiments, a nucleic acid, probe, vector, or otherconstruct of the present invention includes at least about five, atleast about ten, or at least about twenty nucleic acids from a regiondesignated as novel in Table 2. In certain other embodiments, a nucleicacid of the present invention includes at least about five, at leastabout ten, or at least about twenty nucleic acids which are not includedin the clones whose accession numbers are listed in Table 2.

[0095] The invention includes within its scope a polynucleotide havingthe nucleotide sequence of nucleic acid obtained from this biologicalmaterial, wherein the nucleic acid hybridizes under stringent conditions(at least about 4×SSC at 65° C., or at least about 4×SSC at 42° C.; see,for example, U.S. Pat. No. 5,707,829, incorporated herein by reference)with at least 15 contiguous nucleotides of at least one of SEQ ID Nos.1-544. By this is intended that when at least 15 contiguous nucleotidesof one of SEQ ID Nos. 1-544 is used as a probe, the probe willpreferentially hybridize with a gene or mRNA (of the biologicalmaterial) comprising the complementary sequence, allowing theidentification and retrieval of the nucleic acids of the biologicalmaterial that uniquely hybridize to the selected probe. Probes from morethan one of SEQ ID Nos. 1-544 will hybridize with the same gene or mRNAif the cDNA from which they were derived corresponds to one mRNA. Probesof more than 15 nucleotides can be used, but 15 nucleotides representsenough sequence for unique identification.

[0096] Because the present nucleic acids represent partial mRNAtranscripts, two or more nucleic acids of the invention may representdifferent regions of the same mRNA transcript and the same gene. Thus,if two or more of SEQ ID Nos. 1-544 are identified as belonging to thesame clone, then either sequence can be used to obtain the full-lengthmRNA or gene.

[0097] Nucleic acid-related polynucleotides can also be isolated fromcDNA libraries. These libraries are preferably prepared from mRNA ofhuman colon cells, more preferably, human colon cancer specific tissue,designated as the DE clones in the appended Tables. In anotherembodiment the nucleic acids are isolated from libraries prepared fromnormal colon specific tissue, designated herein as PA clones in theappended Tables. In yet another embodiment, this invention disclosesnucleic acid sequences that can be isolated from both libraries preparedfrom a human colon adenocarcinoma cell line, SW480, as well as fromlibraries prepared from either normal colon specific tissue or fromcolon cancer specific tissue. These sequences are listed in Table 3.Alignment of SEQ ID Nos. 1-544, as described above, can indicated that acell line or tissue source of a related protein or polynucleotide canalso be used as a source of the nucleic acid-related cDNA.

[0098] Techniques for producing and probing nucleic acid sequencelibraries are described, for example, in Sambrook et al., “MolecularCloning: A Laboratory Manual” (New York, Cold Spring Harbor Laboratory,1989). The cDNA can be prepared by using primers based on a sequencefrom SEQ ID Nos. 1-544. In one embodiment, the cDNA library can be madefrom only poly-adenylated mRNA. Thus, poly-T primers can be used toprepare cDNA from the mRNA. Alignment of SEQ ID Nos. 1-544 can result inidentification of a related polypeptide or polynucleotide. Some of thepolynucleotides disclosed herein contains repetitive regions that weresubject to masking during the search procedures. The information aboutthe repetitive regions is discussed below.

[0099] Constructs of polynucleotides having sequences of SEQ ID Nos.1-544 can be generated synthetically. Alternatively, single-stepassembly of a gene and entire plasmid from large numbers ofoligodeoxyribonucleotides is described by Stemmer et al., Gene(Amsterdam) (1995) 164(1):49-53. In this method, assembly PCR (thesynthesis of long DNA sequences from large numbers ofoligodeoxyribonucleotides (oligos)) is described. The method is derivedfrom DNA shuffling (Stemmer, Nature (1994) 370:389-391), and does notrely on DNA ligase, but instead relies on DNA polymerase to buildincreasingly longer DNA fragments during the assembly process. Forexample, a 1.1-kb fragment containing the TEM-1 beta-lactamase-encodinggene (bla) can be assembled in a single reaction from a total of 56oligos, each 40 nucleotides (nt) in length. The synthetic gene can bePCR amplified and cloned in a vector containing thetetracycline-resistance gene (Tc-R) as the sole selectable marker.Without relying on ampicillin (Ap) selection, 76% of the Tc-R colonieswere Ap-R, making this approach a general method for the rapid andcost-effective synthesis of any gene.

[0100] IV. Identification of Functional and Structural Motifs of NovelGenes Using Art-Recognized Methods

[0101] Translations of the nucleotide sequence of the nucleic acids,cDNAs, or full genes can be aligned with individual known sequences.Similarity with individual sequences can be used to determine theactivity of the polypeptides encoded by the polynucleotides of theinvention. For example, sequences that show similarity with a chemokinesequence may exhibit chemokine activities. Also, sequences exhibitingsimilarity with more than one individual sequence may exhibit activitiesthat are characteristic of either or both individual sequences.

[0102] The full length sequences and fragments of the polynucleotidesequences of the nearest neighbors can be used as probes and primers toidentify and isolate the full length sequence of the nucleic acid. Thenearest neighbors can indicate a tissue or cell type to be used toconstruct a library for the full-length sequences of the nucleic acid.

[0103] Typically, the nucleic acids are translated in all six frames todetermine the best alignment with the individual sequences. Thesequences disclosed herein in the Sequence Listing are in a 5′ to 3′orientation and translation in three frames can be sufficient (with afew specific exceptions as described in the Examples). These amino acidsequences are referred to, generally, as query sequences, which will bealigned with the individual sequences.

[0104] Nucleic acid sequences can be compared with known genes by any ofthe methods disclosed above. Results of individual and query sequencealignments can be divided into three categories: high similarity, weaksimilarity, and no similarity. Individual alignment results ranging fromhigh similarity to weak similarity provide a basis for determiningpolypeptide activity and/or structure.

[0105] Parameters for categorizing individual results include:percentage of the alignment region length where the strongest alignmentis found, percent sequence identity, and p value.

[0106] The percentage of the alignment region length is calculated bycounting the number of residues of the individual sequence found in theregion of strongest alignment. This number is divided by the totalresidue length of the query sequence to find a percentage. An example isshown below: Query sequence: ASNPERTMIPVTRVGLIRYM     |   ||| |||| |||Individual sequence: YMMTEYLAIPV.RVGLPRYM 1   5   10    15

[0107] The region of alignment begins at amino acid 9 and ends at aminoacid 19. The total length of the query sequence is 20 amino acids. Thepercent of the alignment region length is {fraction (11/20)} or 55%.

[0108] Percent sequence identity is calculated by counting the number ofamino acid matches between the query and individual sequence anddividing total number of matches by the number of residues of theindividual sequence found in the region of strongest alignment. For theexample above, the percent identity would be 10 matches divided by 11amino acids, or approximately 90.9%.

[0109] P value is the probability that the alignment was produced bychance. For a single alignment, the p value can be calculated accordingto Karlin et al., Proc. Natl. Acad. Sci. 87: 2264 (1990) and Karlin etal., Proc. Natl. Acad. Sci. 90: (1993). The p value of multiplealignments using the same query sequence can be calculated using anheuristic approach described in Altschul et al, Nat. Genet. 6: 119(1994). Alignment programs such as BLAST program can calculate the pvalue.

[0110] The boundaries of the region where the sequences align can bedetermined according to Doolittle, Methods in Enzymology, supra; BLASTor FASTA programs; or by determining the area where the sequenceidentity is highest.

[0111] Another factor to consider for determining identity or similarityis the location of the similarity or identity. Strong local alignmentcan indicate similarity even if the length of alignment is short.Sequence identity scattered throughout the length of the query sequencealso can indicate a similarity between the query and profile sequences.

High Similarity

[0112] For the alignment results to be considered high similarity, thepercent of the alignment region length, typically, is at least about 55%of total length query sequence; more typically, at least about 58%; evenmore typically; at least about 60% of the total residue length of thequery sequence. Usually, percent length of the alignment region can beas much as about 62%; more usually, as much as about 64%; even moreusually, as much as about 66%.

[0113] Further, for high similarity, the region of alignment, typically,exhibits at least about 75% of sequence identity; more typically, atleast about 78%; even more typically; at least about 80% sequenceidentity. Usually, percent sequence identity can be as much as about82%; more usually, as much as about 84%; even more usually, as much asabout 86%.

[0114] The p value is used in conjunction with these methods. If highsimilarity is found, the query sequence is considered to have highsimilarity with a profile sequence when the p value is less than orequal to about 10⁻²; more usually; less than or equal to about 10⁻³;even more usually; less than or equal to about 10⁻⁴. More typically, thep value is no more than about 10⁻⁵; more typically; no more than orequal to about 10⁻¹⁰; even more typically; no more than or equal toabout 10⁻¹⁵ for the query sequence to be considered high similarity.

Weak Similarity

[0115] For the alignment results to be considered weak similarity, thereis no minimum percent length of the alignment region nor minimum lengthof alignment. A better showing of weak similarity is considered when theregion of alignment is, typically, at least about 15 amino acid residuesin length; more typically, at least about 20; even more typically; atleast about 25 amino acid residues in length. Usually, length of thealignment region can be as much as about 30 amino acid residues; moreusually, as much as about 40; even more usually, as much as about 60amino acid residues.

[0116] Further, for weak similarity, the region of alignment, typically,exhibits at least about 35% of sequence identity; more typically, atleast about 40%; even more typically; at least about 45% sequenceidentity. Usually, percent sequence identity can be as much as about50%; more usually, as much as about 55%; even more usually, as much asabout 60%.

[0117] If low similarity is found, the query sequence is considered tohave weak similarity with a profile sequence when the p value is usuallyless than or equal to about 10⁻²; more usually; less than or equal toabout 10⁻³; even more usually; less than or equal to about 10⁻⁴. Moretypically, the p value is no more than about 10⁻⁵; more usually; no morethan or equal to about 10⁻¹⁰; even more usually; no more than or equalto about 10⁻¹⁵ for the query sequence to be considered weak similarity.

Similarity Determined by Sequence Identity

[0118] Sequence identity alone can be used to determine similarity of aquery sequence to an individual sequence and can indicate the activityof the sequence. Such an alignment, preferably, permits gaps to alignsequences. Typically, the query sequence is related to the profilesequence if the sequence identity over the entire query sequence is atleast about 15%; more typically, at least about 20%; even moretypically, at least about 25%; even more typically, at least about 50%.Sequence identity alone as a measure of similarity is most useful whenthe query sequence is usually, at least 80 residues in length; moreusually, 90 residues; even more usually, at least 95 amino acid residuesin length. More typically, similarity can be concluded based on sequenceidentity alone when the query sequence is preferably 100 residues inlength; more preferably, 120 residues in length; even more preferably,150 amino acid residues in length.

Determining Activity from Alignments with Profile and Multiple AlignedSequences

[0119] Translations of the nucleic acids can be aligned with amino acidprofiles that define either protein families or common motifs. Also,translations of the nucleic acids can be aligned to multiple sequencealignments (MSA) comprising the polypeptide sequences of members ofprotein families or motifs. Similarity or identity with profilesequences or MSAs can be used to determine the activity of thepolypeptides encoded by nucleic acids or corresponding cDNA or genes.For example, sequences that show an identity or similarity with achemokine profile or MSA can exhibit chemokine activities.

[0120] Profiles can designed manually by (1) creating a MSA, which is analignment of the amino acid sequence of members that belong to thefamily and (2) constructing a statistical representation of thealignment. Such methods are described, for example, in Birney et al.,Nucl. Acid Res. 24(14: 2730-2739 (1996).

[0121] MSAs of some protein families and motifs are publicly available.For example, these include MSAs of 547 different families and motifs.These MSAs are described also in Sonnhammer et al., Proteins 28: 405-420(1997). Other sources are also available in the world wide web. A briefdescription of these MSAs is reported in Pascarella et al., Prot. Eng.9(3): 249-251 (1996).

[0122] Techniques for building profiles from MSAs are described inSonnhammer et al., supra; Birney et al., supra; and Methods inEnzymology, vol. 266: “Computer Methods for Macromolecular SequenceAnalysis,” 1996, ed. Doolittle, Academic Press, Inc., a division ofHarcourt Brace & Co., San Diego, Calif., USA.

[0123] Similarity between a query sequence and a protein family or motifcan be determined by (a) comparing the query sequence against theprofile and/or (b) aligning the query sequence with the members of thefamily or motif.

[0124] Typically, a program such as Searchwise can be used to comparethe query sequence to the statistical representation of the multiplealignment, also known as a profile. The program is described in Birneyet al., supra. Other techniques to compare the sequence and profile aredescribed in Sonnhammer et al., supra and Doolittle, supra.

[0125] Next, methods described by Feng et al., J. Mol. Evol. 25: 351-360(1987) and Higgins et al., CABIOS 5: 151-153 (1989) can be used alignthe query sequence with the members of a family or motif, also known asa MSA. Computer programs, such as PILEUP, can be used. See Feng et al.,infra.

[0126] The following factors are used to determine if a similaritybetween a query sequence and a profile or MSA exists: (1) number ofconserved residues found in the query sequence, (2) percentage ofconserved residues found in the query sequence, (3) number offrameshifts, and (4) spacing between conserved residues.

[0127] Some alignment programs that both translate and align sequencescan make any number of frameshifts when translating the nucleotidesequence to produce the best alignment. The fewer frameshifts needed toproduce an alignment, the stronger the similarity or identity betweenthe query and profile or MSAs. For example, a weak similarity resultingfrom no frameshifts can be a better indication of activity or structureof a query sequence, than a strong similarity resulting from twoframeshifts. Preferably, three or fewer frameshifts are found in analignment; more preferably two or fewer frameshifts; even morepreferably, one or fewer frameshifts; even more preferably, noframeshifts are found in an alignment of query and profile or MSAs.

[0128] Conserved residues are those amino acids that are found at aparticular position in all or some of the family or motif members. Forexample, most known chemokines contain four conserved cysteines.Alternatively, a position is considered conserved if only a certainclass of amino acids is found in a particular position in all or some ofthe family members. For example, the N-terminal position may contain apositively charged amino acid, such as lysine, arginine, or histidine.

[0129] Typically, a residue of a polypeptide is conserved when a classof amino acids or a single amino acid is found at a particular positionin at least about 40% of all class members; more typically, at leastabout 50%; even more typically, at least about 60% of the members.Usually, a residue is conserved when a class or single amino acid isfound in at least about 70% of the members of a family or motif; moreusually, at least about 80%; even more usually, at least about 90%; evenmore usually, at least about 95%.

[0130] A residue is considered conserved when three unrelated aminoacids are found at a particular position in the some or all of themembers; more usually, two unrelated amino acids. These residues areconserved when the unrelated amino acids are found at particularpositions in at least about 40% of all class member; more typically, atleast about 50%; even more typically, at least about 60% of the members.Usually, a residue is conserved when a class or single amino acid isfound in at least about 70% of the members of a family or motif; moreusually, at least about 80%; even more usually, at least about 90%; evenmore usually, at least about 95%.

[0131] A query sequence has similarity to a profile or MSA when thequery sequence comprises at least about 25% of the conserved residues ofthe profile or MSA; more usually, at least about 30%; even more usually;at least about 40%. Typically, the query sequence has a strongersimilarity to a profile sequence or MSA when the query sequencecomprises at least about 45% of the conserved residues of the profile orMSA; more typically, at least about 50%; even more typically; at leastabout 55%.

[0132] V. Probes and Primers

[0133] The nucleotide sequences determined from the cloning of genesfrom tumor cells, especially colon cancer cell lines and tissues willfurther allow for the generation of probes and primers designed foridentifying and/or cloning homologs in other cell types, e.g., fromother tissues, as well as homologs from other mammalian organisms.Nucleotide sequences useful as probes/primers may include all or aportion of the sequences listed in SEQ ID Nos. 1-544 or sequencescomplementary thereto or sequences which hybridize under stringentconditions to all or a portion of SEQ ID Nos. 1-544. For instance, thepresent invention also provides a probe/primer comprising asubstantially purified oligonucleotide, which oligonucleotide comprisinga nucleotide sequence that hybridizes under stringent conditions to atleast approximately 12, preferably 25, more preferably 40, 50, or 75consecutive nucleotides up to the full length of the sense or anti-sensesequence selected from the group consisting of SEQ ID Nos. 1-544,preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, ora sequence complementary thereto, or naturally occurring mutantsthereof. For instance, primers based on a nucleic acid represented inSEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferablySEQ ID Nos. 1-35, or a sequence complementary thereto, can be used inPCR reactions to clone homologs of that sequence.

[0134] In yet another embodiment, the invention provides probes/primerscomprising a nucleotide sequence that hybridizes under moderatelystringent conditions to at least approximately 12, 16, 25, 40, 50 or 75consecutive nucleotides up to the full length of the sense or antisensesequence selected from the group consisting of SEQ ID Nos. 1-544,preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, ornaturally occurring mutants thereof.

[0135] In particular, these probes are useful because they provide amethod for detecting mutations in wild-type genes of the presentinvention. Nucleic acid probes which are complementary to a wild-typegene of the present invention and can form mismatches with mutant genesare provided, allowing for detection by enzymatic or chemical cleavageor by shifts in electrophoretic mobility.

[0136] Likewise, probes based on the subject sequences can be used todetect transcripts or genomic sequences encoding the same or homologousproteins, for use, for example, in prognostic or diagnostic assays. Inpreferred embodiments, the probe further comprises a label groupattached thereto and able to be detected, e.g., the label group isselected from radioisotopes, fluorescent compounds, chemiluminescentcompounds, enzymes, and enzyme co-factors.

[0137] Full-length cDNA molecules comprising the disclosed nucleic acidsare obtained as follows. A subject nucleic acid or a portion thereofcomprising at least about 12, 15, 18, or 20 nucleotides up to the fulllength of a sequence represented in SEQ ID Nos. 1-544, preferably SEQ IDNos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequencecomplementary thereto, may be used as a hybridization probe to detecthybridizing members of a cDNA library using probe design methods,cloning methods, and clone selection techniques as described in U.S.Pat. No. 5,654,173, “Secreted Proteins and Polynucleotides EncodingThem,” incorporated herein by reference. Libraries of cDNA may be madefrom selected tissues, such as normal or tumor tissue, or from tissuesof a mammal treated with, for example, a pharmaceutical agent.Preferably, the tissue is the same as that used to generate the nucleicacids, as both the nucleic acid and the cDNA represent expressed genes.Most preferably, the cDNA library is made from the biological materialdescribed herein in the Examples. Alternatively, many cDNA libraries areavailable commercially. (Sambrook et al., Molecular Cloning: ALaboratory Manual, 2nd Ed. (Cold Spring Harbor Press, Cold SpringHarbor, N.Y. 1989). The choice of cell type for library construction maybe made after the identity of the protein encoded by the nucleicacid-related gene is known. This will indicate which tissue and celltypes are likely to express the related gene, thereby containing themRNA for generating the cDNA.

[0138] Members of the library that are larger than the nucleic acid, andpreferably that contain the whole sequence of the native message, may beobtained. To confirm that the entire cDNA has been obtained, RNAprotection experiments may be performed as follows. Hybridization of afull-length cDNA to an mRNA may protect the RNA from RNase degradation.If the cDNA is not full length, then the portions of the mRNA that arenot hybridized may be subject to RNase degradation. This may be assayed,as is known in the art, by changes in electrophoretic mobility onpolyacrylamide gels, or by detection of released monoribonucleotides.Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed. (ColdSpring Harbor Press, Cold Spring Harbor, N.Y. 1989). In order to obtainadditional sequences 5′ to the end of a partial cDNA, 5′ RACE (PCRProtocols: A Guide to Methods and Applications (Academic Press, Inc.1990)) may be performed.

[0139] Genomic DNA may be isolated using nucleic acids in a mannersimilar to the isolation of full-length cDNAs. Briefly, the nucleicacids, or portions thereof, may be used as probes to libraries ofgenomic DNA. Preferably, the library is obtained from the cell type thatwas used to generate the nucleic acids. Most preferably, the genomic DNAis obtained from the biological material described herein in theExample. Such libraries may be in vectors suitable for carrying largesegments of a genome, such as P1 or YAC, as described in detail inSambrook et al., 9.4-9.30. In addition, genomic sequences can beisolated from human BAC libraries, which are commercially available fromResearch Genetics, Inc., Huntville, Ala., USA, for example. In order toobtain additional 5′ or 3′ sequences, chromosome walking may beperformed, as described in Sambrook et al., such that adjacent andoverlapping fragments of genomic DNA are isolated. These may be mappedand pieced together, as is known in the art, using restriction digestionenzymes and DNA ligase.

[0140] Using the nucleic acids of the invention, corresponding fulllength genes can be isolated using both classical and PCR methods toconstruct and probe cDNA libraries. Using either method, Northern blots,preferably, may be performed on a number of cell types to determinewhich cell lines express the gene of interest at the highest rate.

[0141] Classical methods of constructing cDNA libraries are taught inSambrook et al., supra. With these methods, cDNA can be produced frommRNA and inserted into viral or expression vectors. Typically, librariesof mRNA comprising poly(A) tails can be produced with poly(T) primers.Similarly, cDNA libraries can be produced using the instant sequences asprimers.

[0142] PCR methods may be used to amplify the members of a cDNA librarythat comprise the desired insert. In this case, the desired insert maycontain sequence from the full length cDNA that corresponds to theinstant nucleic acids. Such PCR methods include gene trapping and RACEmethods.

[0143] Gene trapping may entail inserting a member of a cDNA libraryinto a vector. The vector then may be denatured to produce singlestranded molecules. Next, a substrate-bound probe, such a biotinylatedoligo, may be used to trap cDNA inserts of interest. Biotinylated probescan be linked to an avidin-bound solid substrate. PCR methods can beused to amplify the trapped cDNA. To trap sequences corresponding to thefull length genes, the labeled probe sequence may be based on thenucleic acids of the invention, e.g., SEQ ID Nos. 1-168, preferably SEQID Nos. 1-35, or a sequence complementary thereto. Random primers orprimers specific to the library vector can be used to amplify thetrapped cDNA. Such gene trapping techniques are described in Gruber etal., PCT WO 95/04745 and Gruber et al., U.S. Pat. No. 5,500,356. Kitsare commercially available to perform gene trapping experiments from,for example, Life Technologies, Gaithersburg, Md., USA. “Rapidamplification of cDNA ends,” or RACE, is a PCR method of amplifyingcDNAs from a number of different RNAs. The cDNAs may be ligated to anoligonucleotide linker and amplified by PCR using two primers. Oneprimer may be based on sequence from the instant nucleic acids, forwhich full length sequence is desired, and a second primer may comprisea sequence that hybridizes to the oligonucleotide linker to amplify thecDNA. A description of this method is reported in PCT Pub. No. WO97/19110.

[0144] In preferred embodiments of RACE, a common primer may be designedto anneal to an arbitrary adaptor sequence ligated to cDNA ends (Apteand Siebert, Biotechniques 15:890-893, 1993; Edwards et al., Nuc. AcidsRes. 19:5227-5232, 1991). When a single gene-specific RACE primer ispaired with the common primer, preferential amplification of sequencesbetween the single gene specific primer and the common primer occurs.Commercial cDNA pools modified for use in RACE are available.

[0145] Another PCR-based method generates full-length cDNA library withanchored ends without specific knowledge of the cDNA sequence. Themethod uses lock-docking primers (I-VI), where one primer, poly TV(I-III) locks over the polyA tail of eukaryotic mRNA producing firststrand synthesis and a second primer, polyGH (IV-VI) locks onto thepolyC tail added by terminal deoxynucleotidyl transferase (TdT). Thismethod is described in PCT Pub. No. WO 96/40998.

[0146] The promoter region of a gene generally is located 5′ to theinitiation site for RNA polymerase II. Hundreds of promoter regionscontain the “TATA” box, a sequence such as TATTA or TATAA, which issensitive to mutations. The promoter region can be obtained byperforming 5′ RACE using a primer from the coding region of the gene.Alternatively, the cDNA can be used as a probe for the genomic sequence,and the region 5′ to the coding region is identified by “walking up.” Ifthe gene is highly expressed or differentially expressed, the promoterfrom the gene may be of use in a regulatory construct for a heterologousgene.

[0147] Once the full-length cDNA or gene is obtained, DNA encodingvariants can be prepared by site-directed mutagenesis, described indetail in Sambrook et al., 15.3-15.63. The choice of codon or nucleotideto be replaced can be based on the disclosure herein on optional changesin amino acids to achieve altered protein structure and/or function.

[0148] As an alternative method to obtaining DNA or RNA from abiological material, nucleic acid comprising nucleotides having thesequence of one or more nucleic acids of the invention can besynthesized. Thus, the invention encompasses nucleic acid moleculesranging in length from 12 nucleotides (corresponding to at least 12contiguous nucleotides which hybridize under stringent conditions to orare at least 80% identical to a nucleic acid represented by one of SEQID Nos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ IDNos. 1-35, or a sequence complementary thereto) up to a maximum lengthsuitable for one or more biological manipulations, including replicationand expression, of the nucleic acid molecule. The invention includes butis not limited to (a) nucleic acid having the size of a full gene, andcomprising at least one of SEQ ID Nos. 1-544, preferably SEQ ID Nos.1-168, even more preferably SEQ ID Nos. 1-35, or a sequencecomplementary thereto; (b) the nucleic acid of (a) also comprising atleast one additional gene, operably linked to permit expression of afusion protein; (c) an expression vector comprising (a) or (b); (d) aplasmid comprising (a) or (b); and (e) a recombinant viral particlecomprising (a) or (b). Construction of (a) can be accomplished asdescribed below in part IV.

[0149] The sequence of a nucleic acid of the present invention is notlimited and can be any sequence of A, T, G, and/or C (for DNA) and A, U,G, and/or C (for RNA) or modified bases thereof, including inosine andpseudouridine. The choice of sequence will depend on the desiredfunction and can be dictated by coding regions desired, the intron-likeregions desired, and the regulatory regions desired.

[0150] VI. Vectors Carrying Nucleic Acids of the Present Invention

[0151] The invention further provides plasmids and vectors, which can beused to express a gene in a host cell. The host cell may be anyprokaryotic or eukaryotic cell. Thus, a nucleotide sequence derived fromany one of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168, even morepreferably SEQ ID Nos. 1-35, or a sequence complementary thereto,encoding all or a selected portion of a protein, can be used to producea recombinant form of an polypeptide via microbial or eukaryoticcellular processes. Ligating the polynucleotide sequence into a geneconstruct, such as an expression vector, and transforming ortransfecting into hosts, either eukaryotic (yeast, avian, insect ormammalian) or prokaryotic (bacterial cells), are standard procedureswell known in the art.

[0152] Vectors that allow expression of a nucleic acid in a cell arereferred to as expression vectors. Typically, expression vectors containa nucleic acid operably linked to at least one transcriptionalregulatory sequence. Regulatory sequences are art-recognized and areselected to direct expression of the subject nucleic acids.Transcriptional regulatory sequences are described in Goeddel; GeneExpression Technology: Methods in Enzymology 185, Academic Press, SanDiego, Calif. (1990). In one embodiment, the expression vector includesa recombinant gene encoding a peptide having an agonistic activity of asubject polypeptide, or alternatively, encoding a peptide which is anantagonistic form of a subject polypeptide.

[0153] The choice of plasmid will depend on the type of cell in whichpropagation is desired and the purpose of propagation. Certain vectorsare useful for amplifying and making large amounts of the desired DNAsequence. Other vectors are suitable for expression in cells in culture.Still other vectors are suitable for transfer and expression in cells ina whole animal or person. The choice of appropriate vector is wellwithin the skill of the art. Many such vectors are availablecommercially. The nucleic acid or full-length gene is inserted into avector typically by means of DNA ligase attachment to a cleavedrestriction enzyme site in the vector. Alternatively, the desirednucleotide sequence may be inserted by homologous recombination in vivo.Typically this is accomplished by attaching regions of homology to thevector on the flanks of the desired nucleotide sequence. Regions ofhomology are added by ligation of oligonucleotides, or by polymerasechain reaction using primers comprising both the region of homology anda portion of the desired nucleotide sequence.

[0154] Nucleic acids or full-length genes are linked to regulatorysequences as appropriate to obtain the desired expression properties.These may include promoters (attached either at the 5′ end of the sensestrand or at the 3′ end of the antisense strand), enhancers,terminators, operators, repressors, and inducers. The promoters may beregulated or constitutive. In some situations it may be desirable to useconditionally active promoters, such as tissue-specific or developmentalstage-specific promoters. These are linked to the desired nucleotidesequence using the techniques described above for linkage to vectors.Any techniques known in the art may be used.

[0155] When any of the above host cells, or other appropriate host cellsor organisms, are used to replicate and/or express the polynucleotidesor nucleic acids of the invention, the resulting replicated nucleicacid, RNA, expressed protein or polypeptide, is within the scope of theinvention as a product of the host cell or organism. The product isrecovered by any appropriate means known in the art.

[0156] Once the gene corresponding to the nucleic acid is identified,its expression can be regulated in the cell to which the gene is native.For example, an endogenous gene of a cell can be regulated by anexogenous regulatory sequence as disclosed in U.S. Pat. No. 5,641,670,“Protein Production and Protein Delivery.”

[0157] A number of vectors exist for the expression of recombinantproteins in yeast (see, for example, Broach et al. (1983) inExperimental Manipulation of Gene Expression, ed. M. Inouye, AcademicPress, p. 83, incorporated by reference herein). In addition, drugresistance markers such as ampicillin can be used. In an illustrativeembodiment, a polypeptide is produced recombinantly utilizing anexpression vector generated by sub-cloning one of the nucleic acidsrepresented in one of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168,even more preferably SEQ ID Nos. 1-35, or a sequence complementarythereto.

[0158] The preferred mammalian expression vectors contain bothprokaryotic sequences, to facilitate the propagation of the vector inbacteria, and one or more eukaryotic transcription units that areexpressed in eukaryotic cells. The various methods employed in thepreparation of plasmids and transformation of host organisms are wellknown in the art. For other suitable expression systems for bothprokaryotic and eukaryotic cells, as well as general recombinantprocedures, see Molecular Cloning: A Laboratory Manual, 2^(nd) Ed., ed.by Sambrook, Fritsch and Maniatis (Cold Spring Harbor Laboratory Press:1989) Chapters 16 and 17. When it is desirable to express only a portionof a gene, e.g., a truncation mutant, it may be necessary to add a startcodon (ATG) to the oligonucleotide fragment containing the desiredsequence to be expressed. It is well known in the art that a methionineat the N-terminal position can be enzymatically cleaved by the use ofthe enzyme methionine aminopeptidase (MAP). MAP has been cloned from E.coli (Ben-Bassat et al (1987) J. Bacteriol. 169:751-757) and Salmonellatyphimurium and its in vitro activity has been demonstrated onrecombinant proteins (Miller et al. (1987) PNAS 84:2718-1722).Therefore, removal of an N-terminal methionine, if desired, can beachieved either in vivo by expressing polypeptides in a host whichproduces MAP (e.g., E. coli or CM89 or S. cerevisiae), or in vitro byuse of purified MAP (e.g., procedure of Miller et al., supra).

[0159] Moreover, the nucleic acid constructs of the present inventioncan also be used as part of a gene therapy protocol to deliver nucleicacids such as antisense nucleic acids. Thus, another aspect of theinvention features expression vectors for in vivo or in vitrotransfection with an antisense oligonucleotide.

[0160] In addition to viral transfer methods, non-viral methods can alsobe employed to introduce a subject nucleic acid, e.g., a sequencerepresented by one of SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168,even more preferably SEQ ID Nos. 1-35, or a sequence complementarythereto, into the tissue of an animal. Most nonviral methods of genetransfer rely on normal mechanisms used by mammalian cells for theuptake and intracellular transport of macromolecules. In preferredembodiments, non-viral targeting means of the present invention rely onendocytic pathways for the uptake of the subject nucleic acid by thetargeted cell. Exemplary targeting means of this type include liposomalderived systems, polylysine conjugates, and artificial viral envelopes.

[0161] A nucleic acid of any of SEQ ID Nos. 1-544, preferably SEQ IDNos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequencecomplementary thereto, the corresponding cDNA, or the full-length genemay be used to express the partial or complete gene product. Appropriatenucleic acid constructs are purified using standard recombinant DNAtechniques as described in, for example, Sambrook et al., (1989)Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring HarborPress, Cold Spring Harbor, N.Y.), and under current regulationsdescribed in United States Dept. of HHS, National Institute of Health(NIH) Guidelines for Recombinant DNA Research. The polypeptides encodedby the nucleic acid may be expressed in any expression system,including, for example, bacterial, yeast, insect, amphibian andmammalian systems. Suitable vectors and host cells are described in U.S.Pat. No. 5,654,173.

[0162] Bacteria. Expression systems in bacteria include those describedin Chang et al., Nature (1978) 275:615, Goeddel et al., Nature (1979)281:544, Goeddel et al., Nucleic Acids Res. (1980) 8:4057; EP 0 036,776,U.S. Pat. No. 4,551,433, DeBoer et al., Proc. Natl. Acad. Sci. (USA)(1983) 80:2125, and Siebenlist et al., Cell (1980) 20:269.

[0163] Yeast. Expression systems in yeast include those described inHinnen et al., Proc. Natl. Acad. Sci. (USA) (1978) 75:1929; Ito et al.,J. Bacteriol. (1983) 153:163; Kurtz et al., Mol. Cell. Biol. (1986)6:142; Kunze et al., J. Basic Microbiol. (1985) 25:141; Gleeson et al.,J. Gen. Microbiol. (1986) 132:3459, Roggenkamp et al., Mol. Gen. Genet.(1986) 202:302) Das et al., J. Bacteriol. (1984) 158:1165; DeLouvencourt et al., J. Bacteriol. (1983) 154:737, Van den Berg et al.,Bio/Technology (1990) 8:135; Kunze et al., J. Basic Microbiol. (1985)25:141; Cregg et al., Mol Cell. Biol. (1985) 5:3376, U.S. Pat. Nos.4,837,148 and 4,929,555; Beach and Nurse, Nature (1981) 300:706; Davidowet al., Curr. Genet. (1985) 10:380, Gaillardin et al., Curr. Genet.(1985) 10:49, Ballance et al., Biochem. Biophys. Res. Commun. (1983)112:284289; Tilburn et al., Gene (1983) 26:205221, Yelton et al., Proc.Natl. Acad. Sci. (USA) (1984) 81:14701474, Kelly and Hynes, EMBO J.(1985) 4:475479; EP 0 244,234, and WO 91/00357.

[0164] Insect Cells. Expression of heterologous genes in insects isaccomplished as described in U.S. Pat. No. 4,745,051, Friesen et al.(1986) “The Regulation of Baculovirus Gene Expression” in: The MolecularBiology Of Baculoviruses (W. Doerfler, ed.), EP 0 127,839, EP 0 155,476,and Vlak et al., J. Gen. Virol. (1988) 69:765776, Miller et al., Ann.Rev. Microbiol. (1988) 42:177, Carbonell et al., Gene (1988) 73:409,Maeda et al., Nature (1985) 315:592594, LebacqVerheyden et al., Mol.Cell. Biol. (1988) 8:3129; Smith et al., Proc. Natl. Acad. Sci. (USA)(1985) 82:8404, Miyajima et al., Gene (1987) 58:273; and Martin et al.,DNA (1988) 7:99. Numerous baculoviral strains and variants andcorresponding permissive insect host cells from hosts are described inLuckow et al., Bio/Technology (1988) 6:4755, Miller et al., GenericEngineering (Setlow, J. K. et al. eds.), Vol. 8 (Plenum Publishing,1986), pp. 277279, and Maeda et al., Nature, (1985) 315:592-594.

[0165] Mammalian Cells. Mammalian expression is accomplished asdescribed in Dijkema et al., EMBO J. (1985) 4:761, Gorman et al., Proc.Natl. Acad. Sci. (USA) (1982) 79:6777, Boshart et al., Cell (1985)41:521 and U.S. Pat. No. 4,399,216. Other features of mammalianexpression are facilitated as described in Ham and Wallace, Meth. Enz.(1979) 58:44, Barnes and Sato, Anal. Biochem. (1980) 102:255, U.S. Pat.Nos. 4,767,704, 4,657,866, 4,927,762, 4,560,655, WO 90/103430, WO87/00195, and U.S. RE 30,985.

[0166] VII. Therapeutic Nucleic Acid Constructs

[0167] One aspect of the invention relates to the use of the isolatednucleic acid, e.g., SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168,even more preferably SEQ ID Nos. 1-35, or a sequence complementarythereto, in antisense therapy. As used herein, antisense therapy refersto administration or in situ generation of oligonucleotide molecules ortheir derivatives which specifically hybridize (e.g., bind) undercellular conditions with the cellular mRNA and/or genomic DNA, therebyinhibiting transcription and/or translation of that gene. The bindingmay be by conventional base pair complementarity, or, for example, inthe case of binding to DNA duplexes, through specific interactions inthe major groove of the double helix. In general, antisense therapyrefers to the range of techniques generally employed in the art, andincludes any therapy which relies on specific binding to oligonucleotidesequences.

[0168] An antisense construct of the present invention can be delivered,for example, as an expression plasmid which, when transcribed in thecell, produces RNA which is complementary to at least a unique portionof the cellular mRNA. Alternatively, the antisense construct is anoligonucleotide probe which is generated ex vivo and which, whenintroduced into the cell, causes inhibition of expression by hybridizingwith the mRNA and/or genomic sequences of a subject nucleic acid. Sucholigonucleotide probes are preferably modified oligonucleotides whichare resistant to endogenous nucleases, e.g., exonucleases and/orendonucleases, and are therefore stable in vivo. Exemplary nucleic acidmolecules for use as antisense oligonucleotides are phosphoramidate,phosphorothioate and methylphosphonate analogs of DNA (see also U.S.Pat. Nos. 5,176,996; 5,264,564; and 5,256,775). Additionally, generalapproaches to constructing oligomers useful in antisense therapy havebeen reviewed, for example, by Van der Krol et al. (1988) BioTechniques6:958-976; and Stein et al. (1988) Cancer Res 48:2659-2668. With respectto antisense DNA, oligodeoxyribonucleotides derived from the translationinitiation site, e.g., between the −10 and +10 regions of the nucleotidesequence of interest, are preferred.

[0169] Antisense approaches involve the design of oligonucleotides(either DNA or RNA) that are complementary to mRNA. The antisenseoligonucleotides will bind to the mRNA transcripts and preventtranslation. Absolute complementarity, although preferred, is notrequired. In the case of double-stranded antisense nucleic acids, asingle strand of the duplex DNA may thus be tested, or triplex formationmay be assayed. The ability to hybridize will depend on both the degreeof complementarity and the length of the antisense nucleic acid.Generally, the longer the hybridizing nucleic acid, the more basemismatches with an RNA it may contain and still form a stable duplex (ortriplex, as the case may be). One skilled in the art can ascertain atolerable degree of mismatch by use of standard procedures to determinethe melting point of the hybridized complex.

[0170] Oligonucleotides that are complementary to the 5′ end of themRNA, e.g., the 5′ untranslated sequence up to and including the AUGinitiation codon, should work most efficiently at inhibitingtranslation. However, sequences complementary to the 3′ untranslatedsequences of mRNAs have recently been shown to be effective atinhibiting translation of mRNAs as well. (Wagner, R. 1994. Nature372:333). Therefore, oligonucleotides complementary to either the 5′ or3′ untranslated, non-coding regions of a gene could be used in anantisense approach to inhibit translation of endogenous mRNA.Oligonucleotides complementary to the 5′ untranslated region of the mRNAshould include the complement of the AUG start codon. Antisenseoligonucleotides complementary to mRNA coding regions are typically lessefficient inhibitors of translation but could also be used in accordancewith the invention. Whether designed to hybridize to the 5′, 3′, orcoding region of subject mRNA, antisense nucleic acids should be atleast six nucleotides in length, and are preferably less that about 100and more preferably less than about 50, 25, 17 or 10 nucleotides inlength.

[0171] Regardless of the choice of target sequence, it is preferred thatin vitro studies are first performed to quantitate the ability of theantisense oligonucleotide to quantitate the ability of the antisenseoligonucleotide to inhibit gene expression. It is preferred that thesestudies utilize controls that distinguish between antisense geneinhibition and nonspecific biological effects of oligonucleotides. It isalso preferred that these studies compare levels of the target RNA orprotein with that of an internal control RNA or protein. Additionally,it is envisioned that results obtained using the antisenseoligonucleotide are compared with those obtained using a controloligonucleotide. It is preferred that the control oligonucleotide is ofapproximately the same length as the test oligonucleotide and that thenucleotide sequence of the oligonucleotide differs from the antisensesequence no more than is necessary to prevent specific hybridization tothe target sequence.

[0172] The oligonucleotides can be DNA or RNA or chimeric mixtures orderivatives or modified versions thereof, single-stranded ordouble-stranded. The oligonucleotide can be modified at the base moiety,sugar moiety, or phosphate backbone, for example, to improve stabilityof the molecule, hybridization, etc. The oligonucleotide may includeother appended groups such as peptides (e.g., for targeting host cellreceptors), or agents facilitating transport across the cell membrane(see, e.g., Letsinger et al., 1989, Proc. Natl. Acad. Sci. U.S.A.86:6553-6556; Lemaitre et al., 1987, Proc. Natl. Acad. Sci. 84:648-652;PCT Publication No. WO 88/09810, published Dec. 15, 1988) or theblood-brain barrier (see, e.g., PCT Publication No. WO 89/10134,published Apr. 25, 1988), hybridization-triggered cleavage agents (See,e.g., Krol et al., 1988, BioTechniques 6:958-976), or intercalatingagents (See, e.g., Zon, 1988, Pharm. Res. 5:539-549). To this end, theoligonucleotide may be conjugated to another molecule, e.g., a peptide,hybridization triggered cross-linking agent, transport agent,hybridization-triggered cleavage agent, etc.

[0173] The antisense oligonucleotide may comprise at least one modifiedbase moiety which is selected from the group including but not limitedto 5-fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil,hypoxanthine, xantine, 4-acetylcytosine, 5-(carboxyhydroxytriethyl)uracil, 5-carboxymethylaminomethyl-2-thiouridine,5-carboxymethylaminomethyluracil, dihydrouracil,beta-D-galactosylqueosine, inosine, N6-isopentenyladenine,1-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine,2-methylguanine, 3-methylcytosine, 5-methylcytosine, N6-adenine,7-methylguanine, 5-methylaminomethyluracil,5-methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine,5-methoxycarboxymethyluracil, 5-methoxyuracil,2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid (v),wybutoxosine, pseudouracil, queosine, 2-thiocytosine,5-methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil,uracil-5-oxyacetic acid methylester, uracil-5-oxyacetic acid (v),5-methyl-2-thiouracil, 3-(3-amino-3-N-2-carboxypropyl) uracil, (acp3)w,and 2,6-diaminopurine.

[0174] The antisense oligonucleotide may also comprise at least onemodified sugar moiety selected from the group including but not limitedto arabinose, 2-fluoroarabinose, xylulose, and hexose.

[0175] The antisense oligonucleotide can also contain a neutralpeptide-like backbone. Such molecules are termed peptide nucleic acid(PNA)-oligomers and are described, e.g., in Perry-O'Keefe et al. (1996)Proc. Natl. Acad. Sci. U.S.A. 93:14670 and in Eglom et al. (1993) Nature365:566. One advantage of PNA oligomers is their capability to bind tocomplementary DNA essentially independently from the ionic strength ofthe medium due to the neutral backbone of the DNA. In yet anotherembodiment, the antisense oligonucleotide comprises at least onemodified phosphate backbone selected from the group consisting of aphosphorothioate, a phosphorodithioate, a phosphoramidothioate, aphosphoramidate, a phosphordiamidate, a methylphosphonate, an alkylphosphotriester, and a formacetal or analog thereof.

[0176] In yet a further embodiment, the antisense oligonucleotide is anα-anomeric oligonucleotide. An α-anomeric oligonucleotide forms specificdouble-stranded hybrids with complementary RNA in which, contrary to theusual β-units, the strands run parallel to each other (Gautier et al.,1987, Nucl. Acids Res. 15:6625-6641). The oligonucleotide is a2′-O-methylribonucleotide (Inoue et al., 1987, Nucl. Acids Res.15:6131-12148), or a chimeric RNA-DNA analogue (Inoue et al., 1987, FEBSLett. 215:327-330).

[0177] Oligonucleotides of the invention may be synthesized by standardmethods known in the art, e.g., by use of an automated DNA synthesizer(such as are commercially available from Biosearch, Applied Biosystems,etc.). As examples, phosphorothioate oligonucleotides may be synthesizedby the method of Stein et al. (1988, Nucl. Acids Res. 16:3209),methylphosphonate olgonucleotides can be prepared by use of controlledpore glass polymer supports (Sarin et al., 1988, Proc. Natl. Acad. Sci.U.S.A. 85:7448-7451), etc.

[0178] While antisense nucleotides complementary to a coding regionsequence can be used, those complementary to the transcribeduntranslated region and to the region comprising the initiatingmethionine are most preferred.

[0179] The antisense molecules can be delivered to cells which expressthe target nucleic acid in vivo. A number of methods have been developedfor delivering antisense DNA or RNA to cells; e.g., antisense moleculescan be injected directly into the tissue site, or modified antisensemolecules, designed to target the desired cells (e.g., antisense linkedto peptides or antibodies that specifically bind receptors or antigensexpressed on the target cell surface) can be administered systemically.

[0180] However, it is often difficult to achieve intracellularconcentrations of the antisense sufficient to suppress translation onendogenous mRNAs. Therefore, a preferred approach utilizes a recombinantDNA construct in which the antisense oligonucleotide is placed under thecontrol of a strong pol III or pol II promoter. The use of such aconstruct to transfect target cells in the patient will result in thetranscription of sufficient amounts of single stranded RNAs that willform complementary base pairs with the endogenous transcripts andthereby prevent translation of the target mRNA. For example, a vectorcan be introduced in vivo such that it is taken up by a cell and directsthe transcription of an antisense RNA. Such a vector can remain episomalor become chromosomally integrated, as long as it can be transcribed toproduce the desired antisense RNA. Such vectors can be constructed byrecombinant DNA technology methods standard in the art. Vectors can beplasmid, viral, or others known in the art for replication andexpression in mammalian cells. Expression of the sequence encoding theantisense RNA can be by any promoter known in the art to act inmammalian, preferably human cells. Such promoters can be inducible orconstitutive. Such promoters include but are not limited to: the SV40early promoter region (Bernoist and Chambon, 1981, Nature 290:304-310),the promoter contained in the 3′ long terminal repeat of Rous sarcomavirus (Yamamoto et al., 1980, Cell 22:787-797), the herpes thymidinekinase promoter (Wagner et al., 1981, Proc. Natl. Acad. Sci. U.S.A.78:1441-1445), the regulatory sequences of the metallothionein gene(Brinster et al, 1982, Nature 296:39-42), etc. Any type of plasmid,cosmid, YAC or viral vector can be used to prepare the recombinant DNAconstruct which can be introduced directly into the tissue site; e.g.,the choroid plexus or hypothalamus. Alternatively, viral vectors can beused which selectively infect the desired tissue (e.g., for brain,herpesvirus vectors may be used), in which case administration may beaccomplished by another route (e.g., systemically).

[0181] In another aspect of the invention, ribozyme molecules designedto catalytically cleave target mRNA transcripts can be used to preventtranslation of target mRNA and expression of a target protein (See,e.g., PCT International Publication WO90/11364, published Oct. 4, 1990;Sarver et al., 1990, Science 247:1222-1225 and U.S. Pat. No. 5,093,246).While ribozymes that cleave mRNA at site specific recognition sequencescan be used to destroy target mRNAs, the use of hammerhead ribozymes ispreferred. Hammerhead ribozymes cleave mRNAs at locations dictated byflanking regions that form complementary base pairs with the targetmRNA. The sole requirement is that the target mRNA have the followingsequence of two bases: 5′-UG-3′. The construction and production ofhammerhead ribozymes is well known in the art and is described morefully in Haseloff and Gerlach, 1988, Nature, 334:585-591. Preferably theribozyme is engineered so that the cleavage recognition site is locatednear the 5′ end of the target mRNA; i.e., to increase efficiency andminimize the intracellular accumulation of non-functional mRNAtranscripts.

[0182] The ribozymes of the present invention also include RNAendoribonucleases (hereinafter “Cech-type ribozymes”) such as the onewhich occurs naturally in Tetrahymena thermophila (known as the IVS, orL-19 IVS RNA) and which has been extensively described by Thomas Cechand collaborators (Zaug, et al., 1984, Science, 224:574-578; Zaug andCech, 1986, Science, 231:470-475; Zaug, et al., 1986, Nature,324:429-433; published International patent application No. WO88/04300by University Patents Inc.; Been and Cech, 1986, Cell, 47:207-216). TheCech-type ribozymes have an eight base pair active site which hybridizesto a target RNA sequence whereafter cleavage of the target RNA takesplace. The invention encompasses those Cech-type ribozymes which targeteight base-pair active site sequences that are present in a target gene.

[0183] As in the antisense approach, the ribozymes can be composed ofmodified oligonucleotides (e.g., for improved stability, targeting,etc.) and should be delivered to cells which express the target gene invivo. A preferred method of delivery involves using a DNA construct“encoding” the ribozyme under the control of a strong constitutive polIII or pol II promoter, so that transfected cells will producesufficient quantities of the ribozyme to destroy endogenous messages andinhibit translation. Because ribozymes, unlike antisense molecules, arecatalytic, a lower intracellular concentration is required forefficiency.

[0184] Antisense RNA, DNA, and ribozyme molecules of the invention maybe prepared by any method known in the art for the synthesis of DNA andRNA molecules. These include techniques for chemically synthesizingoligodeoxyribonucleotides and oligoribonucleotides well known in the artsuch as for example solid phase phosphoramidite chemical synthesis.Alternatively, RNA molecules may be generated by in vitro and in vivotranscription of DNA sequences encoding the antisense RNA molecule. SuchDNA sequences may be incorporated into a wide variety of vectors whichincorporate suitable RNA polymerase promoters such as the T7 or SP6polymerase promoters. Alternatively, antisense cDNA constructs thatsynthesize antisense RNA constitutively or inducibly, depending on thepromoter used, can be introduced stably into cell lines.

[0185] Moreover, various well-known modifications to nucleic acidmolecules may be introduced as a means of increasing intracellularstability and half-life. Possible modifications include but are notlimited to the addition of flanking sequences of ribonucleotides ordeoxyribonucleotides to the 5′ and/or 3′ ends of the molecule or the useof phosphorothioate or 2′ O-methyl rather than phosphodiesteraselinkages within the oligodeoxyribonucleotide backbone.

[0186] VIII. Polypeptides of the Present Invention

[0187] The present invention makes available isolated polypeptides whichare isolated from, or otherwise substantially free of other cellularproteins, especially other signal transduction factors and/ortranscription factors which may normally be associated with thepolypeptide. Subject polypeptides of the present invention includepolypeptides encoded by the nucleic acids of SEQ ID Nos. 1-544,preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, ora sequence complementary thereto, or polypeptides encoded by genes ofwhich a sequence in SEQ ID Nos. 1-544, preferably SEQ ID Nos. 1-168,even more preferably SEQ ID Nos. 1-35, or a sequence complementarythereto, is a fragment. Polypeptides of the present invention includethose proteins which are differentially regulated in tumor cells,especially colon cancer-derived cell lines (relative to normal cells,e.g., normal colon tissue and non-colon tissue). In preferredembodiments, the polypeptides are upregulated in tumor cells, especiallycolon cancer cancer-derived cell lines. In other embodiments, thepolypeptides are downregulated in tumor cells, especially coloncancer-derived cell lines. Proteins which are upregulated, such asoncogenes, or downregulated, such as tumor suppressors, in aberrantlyproliferating cells may be targets for diagnostic or therapeutictechniques. For example, upregulation of the cdc2 gene induces mitosis.Overexpression of the myt1 gene, a mitotic deactivator, negativelyregulates the activity of cdc2. Aberrant proliferation may thus beinduced either by upregulating cdc2 or by downregulating myt1.

[0188] The term “substantially free of other cellular proteins” (alsoreferred to herein as “contaminating proteins”) or “substantially pureor purified preparations” are defined as encompassing preparations ofpolypeptides having less than about 20% (by dry weight) contaminatingprotein, and preferably having less than about 5% contaminating protein.Functional forms of the subject polypeptides can be prepared, for thefirst time, as purified preparations by using a cloned nucleic acid asdescribed herein. Full length proteins or fragments corresponding to oneor more particular motifs and/or domains or to arbitrary sizes, forexample, at least about 5, 10, 25, 50, 75, or 100 amino acids in lengthare within the scope of the present invention.

[0189] For example, isolated polypeptides can be encoded by all or aportion of a nucleic acid sequence shown in any of SEQ ID Nos. 1-544,preferably SEQ ID Nos. 1-1 68, even more preferably SEQ ID Nos. 1-35, ora sequence complementary thereto. Isolated peptidyl portions of proteinscan be obtained by screening peptides recombinantly produced from thecorresponding fragment of the nucleic acid encoding such peptides. Inaddition, fragments can be chemically synthesized using techniques knownin the art such as conventional Merrifield solid phase f-Moc or t-Bocchemistry. For example, a polypeptide of the present invention may bearbitrarily divided into fragments of desired length with no overlap ofthe fragments, or preferably divided into overlapping fragments of adesired length. The fragments can be produced (recombinantly or bychemical synthesis) and tested to identify those peptidyl fragmentswhich can function as either agonists or antagonists of a wild-type(e.g., “authentic”) protein.

[0190] Another aspect of the present invention concerns recombinantforms of the subject proteins. Recombinant polypeptides preferred by thepresent invention, in addition to native proteins, as described aboveare encoded by a nucleic acid, which is at least 60%, more preferably atleast 80%, and more preferably 85%, and more preferably 90%, and morepreferably 95% identical to an amino acid sequence encoded by SEQ IDNos. 1-544. Polypeptides which are encoded by a nucleic acid that is atleast about 98-99% identical with the sequence of SEQ ID Nos. 1-544 arealso within the scope of the invention. Also included in the presentinvention are peptide fragments comprising at least a portion of such aprotein.

[0191] In a preferred embodiment, a polypeptide of the present inventionis a mammalian polypeptide and even more preferably a human polypeptide.In particularly preferred embodiment, the polypeptide retains wild-typebioactivity. It will be understood that certain post-translationalmodifications, e.g., phosphorylation and the like, can increase theapparent molecular weight of the polypeptide relative to the unmodifiedpolypeptide chain.

[0192] The present invention further pertains to recombinant forms ofone of the subject polypeptides. Such recombinant polypeptidespreferably are capable of functioning in one of either role of anagonist or antagonist of at least one biological activity of a wild-type(“authentic”) polypeptide of the appended sequence listing. The term“evolutionarily related to”, with respect to amino acid sequences ofproteins, refers to both polypeptides having amino acid sequences whichhave arisen naturally, and also to mutational variants of humanpolypeptides which are derived, for example, by combinatorialmutagenesis.

[0193] In general, polypeptides referred to herein as having an activity(e.g., are “bioactive”) of a protein are defined as polypeptides whichinclude an amino acid sequence encoded by all or a portion of thenucleic acid sequences shown in one of SEQ ID Nos. 1-544, preferably SEQID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequencecomplementary thereto, and which mimic or antagonize all or a portion ofthe biological/biochemical activities of a naturally occurring protein.According to the present invention, a polypeptide has biologicalactivity if it is a specific agonist or antagonist of a naturallyoccurring form of a protein.

[0194] Assays for determining whether a compound, e.g., a protein orvariant thereof, has one or more of the above biological activities arewell known in the art. In certain embodiments, the polypeptides of thepresent invention have activities such as those outlined above.

[0195] In another embodiment, the coding sequences for the polypeptidecan be incorporated as a part of a fusion gene including a nucleotidesequence encoding a different polypeptide. This type of expressionsystem can be useful under conditions where it is desirable to producean immunogenic fragment of a polypeptide (see, for example, EPPublication No: 0259149; and Evans et al. (1989) Nature 339:385; Huanget al. (1988) J. Virol. 62:3855; and Schlienger et al. (1992) J. Virol.66:2). In addition to utilizing fusion proteins to enhanceimmunogenicity, it is widely appreciated that fusion proteins can alsofacilitate the expression of proteins, and, accordingly, can be used inthe expression of the polypeptides of the present invention (see, forexample, Current Protocols in Molecular Biology, eds. Ausubel et al.(N.Y.: John Wiley & Sons, 1991)). In another embodiment, a fusion genecoding for a purification leader sequence, such as apoly-(His)/enterokinase cleavage site sequence at the N-terminus of thedesired portion of the recombinant protein, can allow purification ofthe expressed fusion protein by affinity chromatography using a Ni²⁺metal resin. The purification leader sequence can then be subsequentlyremoved by treatment with enterokinase to provide the purified protein(e.g., see Hochuli et al. (1987) J. Chromatography 411:177; andJanknecht et al PNAS 88:8972).

[0196] Techniques for making fusion genes are known to those skilled inthe art. Essentially, the joining of various DNA fragments coding fordifferent polypeptide sequences is performed in accordance withconventional techniques, employing blunt-ended or stagger-ended terminifor ligation, restriction enzyme digestion to provide for appropriatetermini, filling-in of cohesive ends as appropriate, alkalinephosphatase treatment to avoid undesirable joining, and enzymaticligation. In another embodiment, the fusion gene can be synthesized byconventional techniques including automated DNA synthesizers.Alternatively, PCR amplification of nucleic acid fragments can becarried out using anchor primers which give rise to complementaryoverhangs between two consecutive nucleic acid fragments which cansubsequently be annealed to generate a chimeric nucleic acid sequence(see, for example, Current Protocols in Molecular Biology, eds. Ausubelet al. John Wiley & Sons: 1992).

[0197] The present invention further pertains to methods of producingthe subject polypeptides. For example, a host cell transfected with anucleic acid vector directing expression of a nucleotide sequenceencoding the subject polypeptides can be cultured under appropriateconditions to allow expression of the peptide to occur. Suitable mediafor cell culture are well known in the art. The recombinant polypeptidecan be isolated from cell culture medium, host cells, or both usingtechniques known in the art for purifying proteins includingion-exchange chromatography, gel filtration chromatography,ultrafiltration, electrophoresis, and immunoaffinity purification withantibodies specific for such peptide. In a preferred embodiment, therecombinant polypeptide is a fusion protein containing a domain whichfacilitates its purification, such as GST fusion protein.

[0198] Moreover, it will be generally appreciated that, under certaincircumstances, it may be advantageous to provide homologs of one of thesubject polypeptides which function in a limited capacity as one ofeither an agonist (mimetic) or an antagonist, in order to promote orinhibit only a subset of the biological activities of the naturallyoccurring form of the protein. Thus, specific biological effects can beelicited by treatment with a homolog of limited function, and with fewerside effects relative to treatment with agonists or antagonists whichare directed to all of the biological activities of naturally occurringforms of subject proteins.

[0199] Homologs of each of the subject polypeptide can be generated bymutagenesis, such as by discrete point mutation(s), or by truncation.For instance, mutation can give rise to homologs which retainsubstantially the same, or merely a subset, of the biological activityof the polypeptide from which it was derived. Alternatively,antagonistic forms of the polypeptide can be generated which are able toinhibit the function of the naturally occurring form of the protein,such as by competitively binding to a receptor.

[0200] The recombinant polypeptides of the present invention alsoinclude homologs of the wild-type proteins, such as versions of thoseproteins which are resistant to proteolytic cleavage, for example, dueto mutations which alter ubiquitination or other enzymatic targetingassociated with the protein.

[0201] Polypeptides may also be chemically modified to createderivatives by forming covalent or aggregate conjugates with otherchemical moieties, such as glycosyl groups, lipids, phosphate, acetylgroups and the like. Covalent derivatives of proteins can be prepared bylinking the chemical moieties to functional groups on amino acidsidechains of the protein or at the N-terminus or at the C-terminus ofthe polypeptide.

[0202] Modification of the structure of the subject polypeptides can befor such purposes as enhancing therapeutic or prophylactic efficacy,stability (e.g., ex vivo shelf life and resistance to proteolyticdegradation), or post-translational modifications (e.g., to alterphosphorylation pattern of protein). Such modified peptides, whendesigned to retain at least one activity of the naturally occurring formof the protein, or to produce specific antagonists thereof, areconsidered functional equivalents of the polypeptides described in moredetail herein. Such modified peptides can be produced, for instance, byamino acid substitution, deletion, or addition. The substitutionalvariant may be a substituted conserved amino acid or a substitutednon-conserved amino acid.

[0203] For example, it is reasonable to expect that an isolatedreplacement of a leucine with an isoleucine or valine, an aspartate witha glutamate, a threonine with a serine, or a similar replacement of anamino acid with a structurally related amino acid (i.e., isostericand/or isoelectric mutations) will not have a major effect on thebiological activity of the resulting molecule. Conservative replacementsare those that take place within a family of amino acids that arerelated in their side chains. Genetically encoded amino acids can bedivided into four families: (1) acidic=aspartate, glutamate; (2)basic=lysine, arginine, histidine; (3) nonpolar=alanine, valine,leucine, isoleucine, proline, phenylalanine, methionine, tryptophan; and(4) uncharged polar=glycine, asparagine, glutamine, cysteine, serine,threonine, tyrosine. In similar fashion, the amino acid repertoire canbe grouped as (1) acidic=aspartate, glutamate; (2) basic=lysine,arginine histidine, (3) aliphatic=glycine, alanine, valine, leucine,isoleucine, serine, threonine, with serine and threonine optionally begrouped separately as aliphatic-hydroxyl; (4) aromatic=phenylalanine,tyrosine, tryptophan; (5) amide=asparagine, glutamine; and (6)sulfur-containing=cysteine and methionine. (see, for example,Biochemistry, 2^(nd) ed., Ed. by L. Stryer, W H Freeman and Co.: 1981).Whether a change in the amino acid sequence of a peptide results in afunctional homolog (e.g., functional in the sense that the resultingpolypeptide mimics or antagonizes the wild-type form) can be readilydetermined by assessing the ability of the variant peptide to produce aresponse in cells in a fashion similar to the wild-type protein, orcompetitively inhibit such a response. Polypeptides in which more thanone replacement has taken place can readily be tested in the samemanner. The variant may be designed so as to retain biological activityof a particular region of the protein. In a non-limiting example, Osawaet al., 1994, Biochemistry and Molecular International 34:1003-1009,discusses the actin binding region of a protein from several differentspecies. The actin binding regions of the these species are consideredhomologous based on the fact that they have amino acids that fall within“homologous residue groups.” Homologous residues are judged according tothe following groups (using single letter amino acid designations):STAG; ILVMF; HRK; DEQN; and FYW. For example, an S, a T, an A or a G canbe in a position and the function (in this case actin binding) isretained.

[0204] Additional guidance on amino acid substitution is available fromstudies of protein evolution. Go et al, 1980, Int. J. Peptide ProteinRes. 15:211-224, classified amino acid residue sites as interior orexterior depending on their accessibility. More frequent substitution onexterior sites was confirmed to be general in eight sets of homologousprotein families regardless of their biological functions and thepresence or absence of a prosthetic group. Virtually all types of aminoacid residues had higher mutabilities on the exterior than in theinterior. No correlation between mutability and polarity was observed ofamino acid residues in the interior and exterior, respectively. Aminoacid residues were classified into one of three groups depending ontheir polarity: polar (Arg, Lys, His, Gln, Asn, Asp, and Glu); weakpolar (Ala, Pro, Gly, Thr, and Ser), and nonpolar (Cys, Val, Met, Ile,Leu, Phe, Tyr, and Trp). Amino acid replacements during proteinevolution were very conservative: 88% and 76% of them in the interior orexterior, respectively, were within the same group of the three.Inter-group replacements are such that weak polar residues are replacedmore often by nonpolar residues in the interior and more often by polarresidues on the exterior.

[0205] Querol et al., 1996, Prot. Eng. 2:265-271, provides general rulesfor amino acid substitutions to enhance protein thermostability. Newglycosylation sites can be introduced as discussed in Olsen and Thomsen,1991, J. Gen. Microbiol. 137:579-585. An additional disulfide bridge canbe introduced, as discussed by Perry and Wetzel, 1984, Science226:555-557; Pantoliano et al., 1987, Biochemistry 26:2077-2082;Matsumura et al., 1989, Nature 342:291-293; Nishikawa et al., 1990,Protein Eng. 3:443-448; Takagi et al., 1990, J. Biol. Chem.265:6874-6878; Clarke et al., 1993, Biochemistry 32:4322-4329; andWakarchuk et al., 1994, Protein Eng. 7:1379-1386.

[0206] An additional metal binding site can be introduced, according toToma et al., 1991, Biochemistry 30:97-106, and Haezerbrouck et al.,1993, Protein Eng. 6:643-649. Substitutions with prolines in loops canbe made according to Masul et al., 1994, Appl. Env. Microbiol.60:3579-3584; and Hardy et al., FEBS Lett. 317:89-92.

[0207] Cysteine-depleted muteins are considered variants within thescope of the invention. These variants can be constructed according tomethods disclosed in U.S. Pat. No. 4,959,314, which discloses how tosubstitute other amino acids for cysteines, and how to determinebiological activity and effect of the substitution. Such methods aresuitable for proteins according to this invention that have cysteineresidues suitable for such substitutions, for example to eliminatedisulfide bond formation.

[0208] To learn the identity and function of the gene that correlateswith an nucleic acid, the nucleic acids or corresponding amino acidsequences can be screened against profiles of protein families. Suchprofiles focus on common structural motifs among proteins of eachfamily. Publicly available profiles are described above. Additional oralternative profiles are described below.

[0209] In comparing a new nucleic acid with known sequences, severalalignment tools are available. Examples include PileUp, which creates amultiple sequence alignment, and is described in Feng et al., J. Mol.Evol. (1987) 25:351-360. Another method, GAP, uses the alignment methodof Needleman et al., J. Mol. Biol. (1970) 48:443-453. GAP is best suitedfor global alignment of sequences. A third method, BestFit, functions byinserting gaps to maximize the number of matches using the localhomology algorithm of Smith and Waterman, Adv. Appl. Math. (1981)2:482-489.

[0210] Examples of such profiles are described below.

Chemokines

[0211] Chemokines are a family of proteins that have been implicated inlymphocyte trafficking, inflammatory diseases, angiogenesis,hematopoiesis, and viral infection. See, for example, Rollins, Blood(1997) 90(3):909-928, and Wells et al., J. Leuk. Biol. (1997)61:545-550. U.S. Pat. No. 5,605,817 discloses DNA encoding a chemokineexpressed in fetal spleen. U.S. Pat. No. 5,656,724 discloseschemokine-like proteins and methods of use. U.S. Pat. No. 5,602,008discloses DNA encoding a chemokine expressed by liver.

[0212] Mutants of the encoded chemokines are polypeptides having anamino acid sequence that possesses at least one amino acid substitution,addition, or deletion as compared to native chemokines. Fragmentspossess the same amino acid sequence of the native chemokines; mutantsmay lack the amino and/or carboxyl terminal sequences. Fusions aremutants, fragments, or the native chemokines that also include aminoand/or carboxyl terminal amino acid extensions.

[0213] The number or type of the amino acid changes is not critical, noris the length or number of the amino acid deletions, or amino acidextensions that are incorporated in the chemokines as compared to thenative chemokine amino acid sequences. A polynucleotide encoding one ofthese variant polypeptides will retain at least about 80% amino acididentity with at least one known chemokine. Preferably, thesepolypeptides will retain at least about 85% amino acid sequenceidentity, more preferably, at least about 90%; even more preferably, atleast about 95%. In addition, the variants will exhibit at least 80%;preferably about 90%; more preferably about 95% of at least one activityexhibited by a native chemokine. Chemokine activity includesimmunological, biological, receptor binding, and signal transductionfunctions of the native chemokine.

[0214] Chemotaxis. Assays for chemotaxis relating to neutrophils aredescribed in Walz et al., Biochem. Biophys. Res. Commun. (1987) 149:755,Yoshimura et al., Proc. Natl. Acad. Sci. (USA) (1987) 84:9233, andSchroder et al., J. Immunol. (1987) 139:3474; to lymphocytes, Larsen etal., Science (1989) 243:1464, Carr et al., Proc. Natl. Acad. Sci. (USA)(1994) 91:3652; to tumor-infiltrating lymphocytes, Liao et al., J. Exp.Med (1995). 182:1301; to hemopoietic progenitors, Aiuti et al., J. Exp.Med. (1997) 185:111; to monocytes, Valente et al., Biochem. (1988)27:4162; and to natural killer cells, Loetscher et al., J. Immunol.(1996) 156:322, and Allavena et al., Eur. J. Immunol. (1994) 24:3233.

[0215] Assays for determining the biological activity of attractingeosinophils are described in Dahinden et al., J. Exp. Med. (1994)179:751, Weber et al., J. Immunol. (1995) 154:4166, and Noso et al.,Biochem. Biophys. Res. Commun. (1994) 200:1470; for attracting dendriticcells, Sozzani et al., J. Immunol. (1995) 155:3292; for attractingbasophils, in Dahinden et al., J. Exp. Med. (1994) 179:751, Alam et al.,J. Immunol. (1994) 152:1298, Alam et al., J. Exp. Med. (1992) 176:781;and for activating neutrophils, Maghazaci et al., Eur. J Immunol. (1996)26:315, and Taub et al., J. Immunol. (1995) 155:3877. Native chemokinescan act as mitogens for fibroblasts, assayed as described in Mullenbachet al., J. Biol. Chem. (1986) 261:719.

[0216] Receptor Binding. Native chemokines exhibit binding activity witha number of receptors. Description of such receptors and assays todetect binding are described in, for example, Murphy et al., Science(1991) 253:1280; Combadiere et al., J. Biol. Chem. (1995) 270:29671;Daugherty et al., J. Exp. Med. (1996) 183:2349; Samson et al., Biochem.(1996) 35:3362; Raport et al., J. Biol. Chem. (1996) 271:17161;Combadiere et al., J. Leukoc. Biol. (1996) 60:147; Baba et al., J. Biol.Chem. (1997) 23:14893; Yosida et al., J. Biol. Chem. (1997) 272:13803;Arvannitakis et al., Nature (1997) 385:347, and many other assays areknown in the art.

[0217] Kinase Activiation. Assays for kinase activation are described byYen et al., J. Leukoc. Biol. (1997) 61:529; Dubois et al., J. Immunol.(1996) 156:1356; Turner et al., J. Immunol. (1995) 155:2437. Assays forinhibition of angiogenesis or cell proliferation are described in Maioneet al., Science (1990) 247:77. Glycosaminoglycan production can beinduced by native chemokines, assayed as described in Castor et al.,Proc. Natl. Acad. Sci. (USA) (1983) 80:765. Chemokine-mediated histaminerelease from basophils is assayed as described in Dahinden et al., J.Exp. Med. (1989) 170:1787; and White et al., Immunol. Lett. (1989)22:151. Heparin binding is described in Luster et al., J. Exp. Med.(1995) 182:219.

[0218] Dimerization Activity. Chemokines can possess dimerizationactivity, which can be assayed according to Burrows et al., Biochem.(1994) 33:12741; and Zhang et al., Mol. Cell. Biol. (1995) 15:4851.Native chemokines can play a role in the inflammatory response ofviruses. This activity can be assayed as described in Bleul et al.,Nature (1996) 382:829; and Oberlin et al., Nature (1996) 382:833.Exocytosis of monocytes can be promoted by native chemokines. The assayfor such activity is described in Uguccioni et al., Eur. J. Immunol.(1995) 25:64. Native chemokines also can inhibit hemapoietic stem cellproliferation. The method for testing for such activity is reported inGraham et al., Nature (1990) 344:442.

[0219] Death Domain Proteins Several protein families contain deathdomain motifs (Feinstein and Kimchi, TIBS Letters (1995) 20:242-244).Some death domain-containing proteins are implicated in cytotoxicintracellular signaling (Cleveland and Ihle, Cell (1995) 81:479-482, Panet al, Science (1997) 276:111-113, Duan and Dixit, Nature (1997)385:86-89, and Chinnaiyan et al, Science (1996) 274:990-992). U.S. Pat.No. 5,563,039 describes a protein homologous to TRADD (Tumor NecrosisFactor Receptor-1 Associated Death Domain containing protein), andmodifications of the active domain of TRADD that retain the functionalcharacteristics of the protein, as well as apoptosis assays for testingthe function of such death domain containing proteins. U.S. Pat. No.5,658,883 discloses biologically active TGF-B1 peptides. U.S. Pat. No.5,674,734 discloses protein RIP which contains a C-terminal death domainand an N-terminal kinase domain.

[0220] Leukemia Inhibitory Factor (LIF) An LIF profile is constructedfrom sequences of leukemia inhibitor factor, CT-1 (cardiotrophin-1),CNTF (ciliary neurotrophic factor), OSM (oncostatin M), and IL-6(interleukin-6). This profile encompasses a family of secreted cytokinesthat have pleiotropic effects on many cell types including hepatocytes,osteoclasts, neuronal cells and cardiac myocytes, and can be used todetect additional genes encoding such proteins. These molecules are allstructurally related and share a common co-receptor gp130 which mediatesintracellular signal transduction by cytoplasmic tyrosine kinases suchas src.

[0221] Novel proteins related to this family are also likely to besecreted, to activate gp130 and to function in the development of avariety of cell types. Thus new members of this family would becandidates to be developed as growth or survival factors for the celltypes that they stimulate. For more details on this family of cytokines,see Pennica et al, Cytokine and Growth Factor Reviews (1996) 7:81-91.U.S. Pat. No. 5,420,247 discloses LIF receptor and fusion proteins. U.S.Pat. No. 5,443,825 discloses human LIF.

[0222] Angiopoietin Angiopoietin-1 is a secreted ligand of the TIE-2tyrosine kinase; it functions as an angiogenic factor critical fornormal vascular development. Angiopoietin-2 is a natural antagonist ofangiopoietin-1 and thus functions as an anti-angiogenic factor. Thesetwo proteins are structurally similar and activate the same receptor.(Folkman and D'Amore, Cell (1996) 87:1153-1155, and Davis et al, Cell(1996) 87:1161-1169.)

[0223] The angiopoietin molecules are composed of two domains, acoiled-coil region and a region related to fibrinogen. The fibrinogendomain is found in many molecules including ficolin and tesascin, and iswell defined structurally with many members.

[0224] Receptor Protein-Tyro sine Kinases Receptor Protein-Tyro sineKinases or RPTKs are described in Lindberg, Annu. Rev. Cell Biol. (1994)10:251-337.

[0225] Growth Factors: Epidermal Growth Factor (EGF) and FibroblastGrowth Factor (FGF) For a discussion of growth factor superfamilies, seeGrowth Factors: A Practical Approach, Appendix A1 (Ed. McKay and Leigh,Oxford University Press, N.Y., 1993) pp. 237-243.

[0226] The alignments (pretty box) for EGF and FGF are shown in FIGS. 1and 2,

[0227] The alignments (pretty box) for EGF and FGF are shown in FIGS. 1and 2, factor, which is active in the promotion of cell division andwound healing. U.S. Pat. No. 5,439,818 discloses DNA encoding humanrecombinant basic fibroblast growth factor, which is active in woundhealing. U.S. Pat. No. 5,604,293 discloses recombinant human basicfibroblast growth factor, which is useful for wound healing. U.S. Pat.No. 5,410,832 discloses brain-derived and recombinant acidic fibroblastgrowth factor, which act as mitogens for mesoderm andneuroectoderm-derived cells in culture, and promote wound healing insoft tissue, cartilaginous tissue and musculo-skeletal tissue. U.S. Pat.No. 5,387,673 discloses biologically active fragments of FGF that retainactivity.

[0228] Proteins of the TNF Family A profile derived from the TNF familyis created by aligning sequences of the following TNF family members:nerve growth factor (NGF), lymphotoxin, Fas ligand, tumor necrosisfactor (TNF), CD40 ligand, TRAIL, ox40 ligand, 4-1BB ligand, CD27ligand, and CD30 ligand. The profile is designed to identify sequencesof proteins that constitute new members or homologues of this family ofproteins.

[0229] U.S. Pat. No. 5,606,023 discloses mutant TNF proteins; U.S. Pat.No. 5,597,899 and U.S. Pat. No. 5,486,463 disclose TNF muteins; and U.S.Pat. No. 5,652,353 discloses DNA encoding TNF-α muteins.

[0230] Members of the TNF family of proteins have been show in vitro tomultimerize, as described in Burrows et al., Biochem. (1994) 33:12741and Zhang et al., Mol. Cell. Biol. (1995) 154851 and bind receptors asdescribed in Browning et al, J. Immunol. (1994) 147:1230, Androlewicz etal., J. Biol. Chem. (1992) 267:2542, and Crowe et al., Science (1994)264:707.

[0231] In vivo, TNFs proteolytically cleave a target protein asdescribed in Kriegel et al., Cell (1988) 53:45 and Mohler et al., Nature(1994) 370:218 and demonstrate cell proliferation and differentiationactivity. T-cell or thymocyte proliferation is assayed as described inArmitage et al., Eur. J. Immunol. (1992) 22:447; Current Protocols inImmunology, ed. J. E. Coligan et al., 3.1-3.19; Takai et al., J.Immunol. (1986) 137:3494-3500, Bertagnoli et al., J. Immunol. (1990)145:1706-1712, Bertagnoli et al., J. Immunol. (1991) 133:327-340,Bertagnoli et al., J. Immunol. (1992) 149:3778-3783, and Bowman et al.,J. Immunol. (1994) 152:1756-1761. B cell proliferation and Ig secretionare assayed as described in Maliszewski, J. Immunol. (1990)144:3028-3033, and Assays for B Cell Function: In vitro antibodyproduction, Mond and Brunswick, Current Protocols in Immunol., ColiganEd vol 1 pp 3.8.1-3.8.16, John Wiley and Sons, Toronto 1994, Kehrl etal., Science (1987) 238:1144 and Boussiotis et al., PNAS USA (1994)91:7007.

[0232] Other in vivo activities include upregulation of cell surfaceantigens, upregulation of costimulatory molecules, and cellularaggregation/adhesion as described in Barrett et al., J. Immunol. (1991)146:1722; Bjorck et al., Eur. J. Immunol. (1993) 23:1771; Clark et al.,Annu Rev. Immunol. (1991) 9:97; Ranheim et al., J. Exp. Med. (1994)177:925; Yellin, J. Immunol. (1994) 153:666; and Gruss et al., Blood(1994) 84:2305.

[0233] Proliferation and differentiation of hematopoietic andlymphopoietic cells has also been shown in vivo for TNFs, using assaysfor embryonic differentiation and hematopoiesis as described inJohansson et al., Cellular Biology (1995) 15:141-151, Keller et al.,Mol. Cell. Biol. (1993) 13:473-486, McClanahan et al., Blood (1993)81:2903-2915 and using assays to detect stem cell survival anddifferentiation as described in Culture of Hematopoietic Cells, Freshneyet al. eds, pp 1-21, 23-29, 139-162, 163-179, and 265-268, Wiley-Liss,Inc., New York, N.Y., 1994, and Hirajama et al., PNAS USA (1992)89:5907-5911.

[0234] In vivo activities of TNFs also include lymphocyte survival andapoptosis, assayed as described in Darzynkewicz et al., Cytometry (1992)13:795-808; Gorczca et al., Leukemia (1993) 7:659-670; Itoh et al., Cell(1991) 66:233-243; Zacharduk, J. Immunol. (1990) 145:4037-4045; Zamai etal., Cytometry (1993) 14:891-897; and Gorczyca et al., Int'l J. Oncol.(1992) 1:639-648.

[0235] Some members of the TNF family are cleaved from the cell surface;others remain membrane bound. The three-dimensional structure of TNF isdiscussed in Sprang and Eck, Tumor Necrosis Factors; supra.

[0236] TNF proteins include a transmembrane domain. The protein iscleaved into a shorter soluble version, as described in Kriegler et al.,Cell (1988) 53:45-53, Perez et al., Cell (1990) 63:251-258, and Shaw etal., Cell (1986) 46:659-667. The transmembrane domain is between aminoacid 46 and 77 and the cytoplasmic domain is between position 1 and 45on the human form of TNFα. The 3-dimensional motifs of TNF include asandwich of two pleated β-sheets. Each sheet is composed ofanti-parallel α-strands. α-Strands facing each other on opposite sitesof the sandwich are connected by short polypeptide loops, as describedin Van Ostade et al., Protein Engineering (1994) 7(1):5-22, and Spranget al., Tumor Necrosis Factors; supra.

[0237] Residues of the TNF family proteins that are involved in theβ-sheet secondary structure have been identified as described in VanOstade et al., Protein Engineering (1994) 7(1):5-22, and Sprang et al.,Tumor Necrosis Factors; supra.

[0238] TNF receptors are disclosed in U.S. Pat. No. 5,395,760. A profilederived from the TNF receptor family is created by aligning sequences ofthe TNF receptor family, including Apo1/Fas, TNFR I and II, deathreceptor3 (DR3), CD40, ox40, CD27, and CD30. Thus, the profile isdesigned to identify, from the nucleic acids of the invention, sequencesof proteins that constitute new members or homologs of this family ofproteins.

[0239] Tumor necrosis factor receptors exist in two forms in humans: p55TNFR and p75 TNFR, both of which provide intracellular signals uponbinding with a ligand. The extracellular domains of these receptorproteins are cysteine rich. The receptors can remain membrane bound,although some forms of the receptors are cleaved forming solublereceptors. The regulation, diagnostic, prognostic, and therapeutic valueof soluble TNF receptors is discussed in Aderka, Cytokine and GrowthFactor Reviews, (1996) 7(3):231-240.

[0240] PDGF Family U.S. Pat. No. 5,326,695 discloses platelet derivedgrowth factor agonists; bioactive portions of PDGF-B are used asagonists. U.S. Pat. No. 4,845,075 discloses biologically active B-chainhomodimers, and also includes variants and derivatives of the PDGF-Bchain. U.S. Pat. No.5,128,321 discloses PDGF analogs and methods of use.Proteins having the same bioactivity as PDGF are disclosed, including Aand B chain proteins.

[0241] Kinase (Including MKK) Family U.S. Pat. No. 5,650,501 disclosesserine/threonine kinase, associated with mitotic and meiotic celldivision; the protein has a kinase domain in its N-terminal and 3 PESTregions in the C-terminus. U.S. Pat. No. 5,605,825 discloses humanPAK65, a serine protein kinase.

[0242] The foregoing discussion provides a few examples of the proteinprofiles that can be compared with the nucleic acids of the invention.One skilled in the art can use these and other protein profiles toidentify the genes that correlate with the nucleic acids.

[0243] IX. Determining the Function of the Encoded Expression Products

[0244] Ribozymes, antisense constructs, dominant negative mutants, andtriplex formation can be used to determine function of the expressionproduct of an nucleic acid-related gene.

[0245] A. Ribozymes

[0246] Trans-cleaving catalytic RNAs (ribozymes) are RNA moleculespossessing endoribonuclease activity. Ribozymes are specificallydesigned for a particular target, and the target message must contain aspecific nucleotide sequence. They are engineered to cleave any RNAspecies site-specifically in the background of cellular RNA. Thecleavage event renders the mRNA unstable and prevents proteinexpression. Importantly, ribozymes can be used to inhibit expression ofa gene of unknown function for the purpose of determining its functionin an in vitro or in vivo context, by detecting the phenotypic effect.

[0247] One commonly used ribozyme motif is the hammerhead, for which thesubstrate sequence requirements are minimal. Design of the hammerheadribozyme is disclosed in Usman et al., Current Opin. Struct. Biol.(1996) 6:527-533. Usman also discusses the therapeutic uses ofribozymes. Ribozymes can also be prepared and used as described in Longet al., FASEB J. (1993) 7:25; Symons, Ann. Rev. Biochem. (1992) 61:641;Perrotta et al., Biochem. (1992) 31:16-17; Ojwang et al., Proc. Natl.Acad. Sci. (USA) (1992) 89:10802-10806; and U.S. Pat. No. 5,254,678.Ribozyme cleavage of HIV-I RNA is described in U.S. Pat. No. 5,144,019;methods of cleaving RNA using ribozymes is described in U.S. Pat. No.5,116,742; and methods for increasing the specificity of ribozymes aredescribed in U.S. Pat. No. 5,225,337 and Koizumi et al., Nucleic AcidRes. (1989) 17:7059-7071. Preparation and use of ribozyme fragments in ahammerhead structure are also described by Koizumi et al., Nucleic AcidsRes. (1989) 17:7059-7071. Preparation and use of ribozyme fragments in ahairpin structure are described by Chowrira and Burke, Nucleic AcidsRes. (1992) 20:2835. Ribozymes can also be made by rolling transcriptionas described in Daubendiek and Kool, Nat. Biotechnol. (1997)15(3):273-277.

[0248] The hybridizing region of the ribozyme may be modified or may beprepared as a branched structure as described in Horn and Urdea, NucleicAcids Res. (1989) 17:6959-67. The basic structure of the ribozymes mayalso be chemically altered in ways familiar to those skilled in the art,and chemically synthesized ribozymes can be administered as syntheticoligonucleotide derivatives modified by monomeric units. In atherapeutic context, liposome mediated delivery of ribozymes improvescellular uptake, as described in Birikh et al., Eur. J. Biochem. (1997)245:1-16.

[0249] Using the nucleic acid sequences of the invention and methodsknown in the art, ribozymes are designed to specifically bind and cutthe corresponding mRNA species. Ribozymes thus provide a means toinhibit the expression of any of the proteins encoded by the disclosednucleic acids or their full-length genes. The full-length gene need notbe known in order to design and use specific inhibitory ribozymes. Inthe case of a nucleic acid or cDNA of unknown function, ribozymescorresponding to that nucleotide sequence can be tested in vitro forefficacy in cleaving the target transcript. Those ribozymes that effectcleavage in vitro are further tested in vivo. The ribozyme can also beused to generate an animal model for a disease, as described in Birikhet al., Eur. J. Biochem. (1997) 245:1-16. An effective ribozyme is usedto determine the function of the gene of interest by blocking itstranscription and detecting a change in the cell. Where the gene isfound to be a mediator in a disease, an effective ribozyme is designedand delivered in a gene therapy for blocking transcription andexpression of the gene.

[0250] Therapeutic and functional genomic applications of ribozymesproceed beginning with knowledge of a portion of the coding sequence ofthe gene to be inhibited. Thus, for many genes, a partial nucleic acidsequence provides adequate sequence for constructing an effectiveribozyme. A target cleavage site is selected in the target sequence, anda ribozyme is constructed based on the 5′ and 3′ nucleotide sequencesthat flank the cleavage site. Retroviral vectors are engineered toexpress monomeric and multimeric hammerhead ribozymes targeting the mRNAof the target coding sequence. These monomeric and multimeric ribozymesare tested in vitro for an ability to cleave the target mRNA. A cellline is stably transduced with the retroviral vectors expressing theribozymes, and the transduction is confirmed by Northern blot analysisand reverse-transcription polymerase chain reaction (RT-PCR). The cellsare screened for inactivation of the target mRNA by such indicators asreduction of expression of disease markers or reduction of the geneproduct of the target mRNA.

[0251] B. Antisense

[0252] Antisense nucleic acids are designed to specifically bind to RNA,resulting in the formation of RNA-DNA or RNA-RNA hybrids, with an arrestof DNA replication, reverse transcription or messenger RNA translation.Antisense polynucleotides based on a selected nucleic acid sequence caninterfere with expression of the corresponding gene. Antisensepolynucleotides are typically generated within the cell by expressionfrom antisense constructs that contain the antisense nucleic acid strandas the transcribed strand. Antisense nucleic acids will bind and/orinterfere with the translation of nucleic acid-related mRNA. Theexpression products of control cells and cells treated with theantisense construct are compared to detect the protein product of thegene corresponding to the nucleic acid. The protein is isolated andidentified using routine biochemical methods.

[0253] One rationale for using antisense methods to determine thefunction of the gene corresponding to a nucleic acid is the biologicalactivity of antisense therapeutics. Antisense therapy for a variety ofcancers is in clinical phase and has been discussed extensively in theliterature. Reed reviewed antisense therapy directed at the Bcl-2 genein tumors; gene transfer-mediated overexpression of Bcl-2 in tumor celllines conferred resistance to many types of cancer drugs. (Reed, J. C.,N.C.I (1997) 89:988-990). The potential for clinical development ofantisense inhibitors of ras is discussed by Cowsert, L. M., Anti-CancerDrug Design (1997) 12:359-371. Additional important antisense targetsinclude leukemia (Geurtz, A. M., Anti-Cancer Drug Design (1997)12:341-358); human C-ref kinase (Monia, B. P., Anti-Cancer Drug Design(1997) 12:327-339); and protein kinase C (McGraw et al, Anti-Cancer DrugDesign (1997) 12:315-326.

[0254] Given the extensive background literature and clinical experiencein antisense therapy, one skilled in the art can use selected nucleicacids of the invention as additional potential therapeutics. The choiceof nucleic acid can be narrowed by first testing them for binding to“hot spot” regions of the genome of cancerous cells. If a nucleic acidis identified as binding to a “hot spot”, testing the nucleic acid as anantisense compound in the corresponding cancer cells clearly iswarranted.

[0255] Ogunbiyi et al., Gastroenterology (1997) 113(3):761-766 describeprognostic use of allelic loss in colon cancer; Barks et al, Genes,Chromosomes, and Cancer (1997) 19(4):278-285 describe increasedchromosome copy number detected by FISH in malignant melanoma; Nishizakeet al., Genes, Chromosomes, and Cancer (1997) 19(4):267-272 describegenetic alterations in primary breast cancer and their metastases anddirect comparison using modified comparative genome hybridization; andElo et al., Cancer Research (1997) 57(16):3356-3359 disclose that lossof heterozygosity at 16z24.1-q24.2 is significantly associated withmetastatic and aggressive behavior of prostate cancer.

[0256] C. Dominant Negative Mutations

[0257] As an alternative method for identifying function of the nucleicacid-related gene, dominant negative mutations are readily generated forcorresponding proteins that are active as homomultimers. A mutantpolypeptide will interact with wild-type polypeptides (made from theother allele) and form a non-functional multimer. Thus, a mutation is ina substrate-binding domain, a catalytic domain, or a cellularlocalization domain. Preferably, the mutant polypeptide will beoverproduced. Point mutations are made that have such an effect. Inaddition, fusion of different polypeptides of various lengths to theterminus of a protein can yield dominant negative mutants. Generalstrategies are available for making dominant negative mutants. SeeHerskowitz, Nature (1987) 329:219-222. Such a technique can be used forcreating a loss-of-function mutation, which is useful for determiningthe function of a protein.

[0258] D. Triplex Formation

[0259] Endogenous gene expression can also be reduced by inactivating or“knocking out” the gene or its promoter using targeted homologousrecombination. (E.g., see Smithies et al., 1985, Nature 317:230-234;Thomas & Capecchi, 1987, Cell 51:503-512; Thompson et al., 1989 Cell5:313-321; each of which is incorporated by reference herein in itsentirety). For example, a mutant, non-functional gene (or a completelyunrelated DNA sequence) flanked by DNA homologous to the endogenous gene(either the coding regions or regulatory regions of the gene) can beused, with or without a selectable marker and/or a negative selectablemarker, to transfect cells that express that gene in vivo. Insertion ofthe DNA construct, via targeted homologous recombination, results ininactivation of the gene.

[0260] Alternatively, endogenous gene expression can be reduced bytargeting deoxyribonucleotide sequences complementary to the regulatoryregion of the target gene (i.e., the gene promoter and/or enhancers) toform triple helical structures that prevent transcription of the gene intarget cells in the body. (See generally, Helene, C. 1991, AnticancerDrug Des., 6(6):569-84; Helene, C., et al., 1992, Ann, N.Y. Accad. Sci.,660:27-36; and Maher, L. J., 1992, Bioassays 14(12):807-15).

[0261] Nucleic acid molecules to be used in triple helix formation forthe inhibition of transcription are preferably single stranded andcomposed of deoxyribonucleotides. The base composition of theseoligonucleotides should promote triple helix formation via Hoogsteenbase-pairing rules, which generally require sizable stretches of eitherpurines or pyrimidines to be present on one strand of a duplex.Nucleotide sequences may be pyrimidine-based, which will result in TATand CGC triplets across the three associated strands of the resultingtriple helix. The pyrimidine-rich molecules provide base complementarityto a purine-rich region of a single strand of the duplex in a parallelorientation to that strand. In addition, nucleic acid molecules may bechosen that are purine-rich, for example, containing a stretch of Gresidues. These molecules will form a triple helix with a DNA duplexthat is rich in GC pairs, in which the majority of the purine residuesare located on a single strand of the targeted duplex, resulting in CGCtriplets across the three strands in the triplex.

[0262] Alternatively, the potential sequences that can be targeted fortriple helix formation may be increased by creating a so called“switchback” nucleic acid molecule. Switchback molecules are synthesizedin an alternating 5′-3′, 3′-5′ manner, such that they base pair withfirst one strand of a duplex and then the other, eliminating thenecessity for a sizable stretch of either purines or pyrimidines to bepresent on one strand of a duplex.

[0263] Antisense RNA and DNA, ribozyme, and triple helix molecules ofthe invention may be prepared by any method known in the art for thesynthesis of DNA and RNA molecules. These include techniques forchemically synthesizing oligodeoxyribonucleotides andoligoribonucleotides well known in the art such as for example solidphase phosphoramidite chemical synthesis. Alternatively, RNA moleculesmay be generated by in vitro and in vivo transcription of DNA sequencesencoding the antisense RNA molecule. Such DNA sequences may beincorporated into a wide variety of vectors which incorporate suitableRNA polymerase promoters such as the T7 or SP6 polymerase promoters.Alternatively, antisense cDNA constructs that synthesize antisense RNAconstitutively or inducibly, depending on the promoter used, can beintroduced stably into cell lines.

[0264] Moreover, various well known modifications to nucleic acidmolecules may be introduced as a means of increasing intracellularstability and half-life. Possible modifications include but are notlimited to the addition of flanking sequences of ribonucleotides ordeoxyribonucleotides to the 5′ and/or 3′ ends of the molecule or the useof phosphorothioate or 2′ O-methyl rather than phosphodiesteraselinkages within the oligodeoxyribonucleotide backbone.

[0265] X. Diagnostic & Prognostic Assays and Drug Screening Methods

[0266] The present invention provides method for determining whether asubject is at risk for developing a disease or condition characterizedby unwanted cell proliferation by detecting the disclosed biomarkers,i.e., the disclosed nucleic acid markers (SEQ ID NOs:1-544) and/orpolypeptide markers for colon cancer encoded thereby.

[0267] In clinical applications, human tissue samples can be screenedfor the presence and/or absence of the biomarkers identified herein.Such samples could consist of needle biopsy cores, surgical resectionsamples, lymph node tissue, or serum. For example, these methods includeobtaining a biopsy, which is optionally fractionated by cryostatsectioning to enrich tumor cells to about 80% of the total cellpopulation. In certain embodiments, nucleic acids extracted from thesesamples may be amplified using techniques well known in the art. Thelevels of selected markers detected would be compared with statisticallyvalid groups of metastatic, non-metastatic malignant, benign, or normalcolon tissue samples.

[0268] In one embodiment, the diagnostic method comprises determiningwhether a subject has an abnormal mRNA and/or protein level of thedisclosed markers, such as by Northern blot analysis, reversetranscription-polymerase chain reaction (RT-PCR), in situ hybridization,immunoprecipitation, Western blot hybridization, orimmunohistochemistry. According to the method, cells are obtained from asubject and the levels of the disclosed biomarkers, protein or mRNAlevel, is determined and compared to the level of these markers in ahealthy subject. An abnormal level of the biomarker polypeptide or mRNAlevels is likely to be indicative of cancer such as colon cancer.

[0269] Accordingly, in one aspect, the invention provides probes andprimers that are specific to the unique nucleic acid markers disclosedherein. Accordingly, the nucleic acid probes comprise a nucleotidesequence at least 12 nucleotides in length, preferably at least 15nucleotides, more preferably, 25 nucleotides, and most preferably atleast 40 nucleotides, and up to all or nearly all of the coding sequencewhich is complementary to a portion of the coding sequence of a markernucleic acid sequence, which nucleic acid sequence is represented by SEQID Nos:1-544 or a sequence complementary thereto.

[0270] In one embodiment, the method comprises using a nucleic acidprobe to determine the presence of cancerous cells in a tissue from apatient. Specifically, the method comprises:

[0271] 1. providing a nucleic acid probe comprising a nucleotidesequence at least 12 nucleotides in length, preferably at least 15nucleotides, more preferably, 25 nucleotides, and most preferably atleast 40 nucleotides, and up to all or nearly all of the coding sequencewhich is complementary to a portion of the coding sequence of a nucleicacid sequence represented by SEQ ID Nos:1-544 or a sequencecomplementary thereto and is differentially expressed in tumors cells,such as colon cancer cells;

[0272] 2. obtaining a tissue sample from a patient potentiallycomprising cancerous cells;

[0273] 3. providing a second tissue sample containing cellssubstantially all of which are non-cancerous;

[0274] 4. contacting the nucleic acid probe under stringent conditionswith RNA of each of said first and second tissue samples (e.g., in aNorthern blot or in situ hybridization assay); and

[0275] 5. comparing (a) the amount of hybridization of the probe withRNA of the first tissue sample, with (b) the amount of hybridization ofthe probe with RNA of the second tissue sample; wherein a statisticallysignificant difference in the amount of hybridization with the RNA ofthe first tissue sample as compared to the amount of hybridization withthe RNA of the second tissue sample is indicative of the presence ofcancerous cells in the first tissue sample.

[0276] In one aspect, the method comprises in situ hybridization with aprobe derived from a given marker nucleic acid sequence, which nucleicacid sequence is represented by SEQ ID Nos:1-544 or a sequencecomplementary thereto. The method comprises contacting the labeledhybridization probe with a sample of a given type of tissue potentiallycontaining cancerous or pre-cancerous cells as well as normal cells, anddetermining whether the probe labels some cells of the given tissue typeto a degree significantly different (e.g., by at least a factor of two,or at least a factor of five, or at least a factor of twenty, or atleast a factor of fifty) than the degree to which it labels other cellsof the same tissue type.

[0277] Also within the invention is a method of determining thephenotype of a test cell from a given human tissue, e.g., whether thecell is (a) normal, or (b) cancerous or precancerous, by contacting themRNA of a test cell with a nucleic acid probe at least 12 nucleotides inlength, preferably at least 15 nucleotides, more preferably at least 25nucleotides, and most preferably at least 40 nucleotides, and up to allor nearly all of a sequence which is complementary to a portion of thecoding sequence of a nucleic acid sequence represented by SEQ IDNos:1-544 or a sequence complementary thereto, and which isdifferentially expressed in tumor cells as compared to normal cells ofthe given tissue type; and determining the approximate amount ofhybridization of the probe to the mRNA, an amount of hybridizationeither more or less than that seen with the mRNA of a normal cell ofthat tissue type being indicative that the test cell is cancerous orpre-cancerous.

[0278] Alternatively, the above diagnostic assays may be carried outusing antibodies to detect the protein product encoded by the markernucleic acid sequence, which nucleic acid sequence is represented by SEQID Nos:1-544 or a sequence complementary thereto. Accordingly, in oneembodiment, the assay would include contacting the proteins of the testcell with an antibody specific for the gene product of a nucleic acidrepresented by SEQ ID Nos:1-544 or a sequence complementary thereto, themarker nucleic acid being one which is expressed at a given controllevel in normal cells of the same tissue type as the test cell, anddetermining the approximate amount of immunocomplex formation by theantibody and the proteins of the test cell, wherein a statisticallysignificant difference in the amount of the immunocomplex formed withthe proteins of a test cell as compared to a normal cell of the sametissue type is an indication that the test cell is cancerous orpre-cancerous.

[0279] Another such method includes the steps of: providing an antibodyspecific for the gene product of a marker nucleic acid sequencerepresented by SEQ ID Nos 1-544, the gene product being present incancerous tissue of a given tissue type (e.g., colon tissue) at a levelmore or less than the level of the gene product in non-cancerous tissueof the same tissue type; obtaining from a patient a first sample oftissue of the given tissue type, which sample potentially includescancerous cells; providing a second sample of tissue of the same tissuetype (which may be from the same patient or from a normal control, e.g.another individual or cultured cells), this second sample containingnormal cells and essentially no cancerous cells; contacting the antibodywith protein (which may be partially purified, in lysed butunfractionated cells, or in situ) of the first and second samples underconditions permitting immunocomplex formation between the antibody andthe marker nucleic acid sequence product present in the samples; andcomparing (a) the amount of immunocomplex formation in the first sample,with (b) the amount of immunocomplex formation in the second sample,wherein a statistically significant difference in the amount ofimmunocomplex formation in the first sample less as compared to theamount of immunocomplex formation in the second sample is indicative ofthe presence of cancerous cells in the first sample of tissue.

[0280] The subject invention further provides a method of determiningwhether a cell sample obtained from a subject possesses an abnormalamount of marker polypeptide which comprises (a) obtaining a cell samplefrom the subject, (b) quantitatively determining the amount of themarker polypeptide in the sample so obtained, and (c) comparing theamount of the marker polypeptide so determined with a known standard, soas to thereby determine whether the cell sample obtained from thesubject possesses an abnormal amount of the marker polypeptide. Suchmarker polypeptides may be detected by immunohistochemical assays,dot-blot assays, ELISA and the like.

[0281] Immunoassays are commonly used to quantitate the levels ofproteins in cell samples, and many other immunoassay techniques areknown in the art. The invention is not limited to a particular assayprocedure, and therefore is intended to include both homogeneous andheterogeneous procedures. Exemplary immunoassays which can be conductedaccording to the invention include fluorescence polarization immunoassay(FPIA), fluorescence immunoassay (FIA), enzyme immunoassay (EIA),nephelometric inhibition immunoassay (NIA), enzyme linked immunosorbentassay (ELISA), and radioimmunoassay (RIA). An indicator moiety, or labelgroup, can be attached to the subject antibodies and is selected so asto meet the needs of various uses of the method which are often dictatedby the availability of assay equipment and compatible immunoassayprocedures. General techniques to be used in performing the variousimmunoassays noted above are known to those of ordinary skill in theart.

[0282] In another embodiment, the level of the encoded product, i.e.,the product encoded by SEQ ID Nos 1-544 or a sequence complementarythereto, in a biological fluid (e.g., blood or urine) of a patient maybe determined as a way of monitoring the level of expression of themarker nucleic acid sequence in cells of that patient. Such a methodwould include the steps of obtaining a sample of a biological fluid fromthe patient, contacting the sample (or proteins from the sample) with anantibody specific for a encoded marker polypeptide, and determining theamount of immune complex formation by the antibody, with the amount ofimmune complex formation being indicative of the level of the markerencoded product in the sample. This determination is particularlyinstructive when compared to the amount of immune complex formation bythe same antibody in a control sample taken from a normal individual orin one or more samples previously or subsequently obtained from the sameperson.

[0283] In another embodiment, the method can be used to determine theamount of marker polypeptide present in a cell, which in turn can becorrelated with progression of a hyperproliferative disorder, e.g.,colon cancer. The level of the marker polypeptide can be usedpredictively to evaluate whether a sample of cells contains cells whichare, or are predisposed towards becoming, transformed cells. Moreover,the subject method can be used to assess the phenotype of cells whichare known to be transformed, the phenotyping results being useful inplanning a particular therapeutic regimen. For instance, very highlevels of the marker polypeptide in sample cells is a powerfuldiagnostic and prognostic marker for a cancer, such as colon cancer. Theobservation of marker polypeptide level can be utilized in decisionsregarding, e.g., the use of more aggressive therapies.

[0284] As set out above, one aspect of the present invention relates todiagnostic assays for determining, in the context of cells isolated froma patient, if the level of a marker polypeptide is significantly reducedin the sample cells. The term “significantly reduced” refers to a cellphenotype wherein the cell possesses a reduced cellular amount of themarker polypeptide relative to a normal cell of similar tissue origin.For example, a cell may have less than about 50%, 25%, 10%, or 5% of themarker polypeptide that a normal control cell. In particular, the assayevaluates the level of marker polypeptide in the test cells, and,preferably, compares the measured level with marker polypeptide detectedin at least one control cell, e.g., a normal cell and/or a transformedcell of known phenotype.

[0285] Of particular importance to the subject invention is the abilityto quantitate the level of marker polypeptide as determined by thenumber of cells associated with a normal or abnormal marker polypeptidelevel. The number of cells with a particular marker polypeptidephenotype may then be correlated with patient prognosis. In oneembodiment of the invention, the marker polypeptide phenotype of thelesion is determined as a percentage of cells in a biopsy which arefound to have abnormally high/low levels of the marker polypeptide. Suchexpression may be detected by immunohistochemical assays, dot-blotassays, ELISA and the like.

[0286] Where tissue samples are employed, immunohistochemical stainingmay be used to determine the number of cells having the markerpolypeptide phenotype. For such staining, a multiblock of tissue istaken from the biopsy or other tissue sample and subjected toproteolytic hydrolysis, employing such agents as protease K or pepsin.In certain embodiments, it may be desirable to isolate a nuclearfraction from the sample cells and detect the level of the markerpolypeptide in the nuclear fraction.

[0287] The tissue samples are fixed by treatment with a reagent such asformalin, glutaraldehyde, methanol, or the like. The samples are thenincubated with an antibody, preferably a monoclonal antibody, withbinding specificity for the marker polypeptides. This antibody may beconjugated to a label for subsequent detection of binding. Samples areincubated for a time sufficient for formation of the immuno-complexes.Binding of the antibody is then detected by virtue of a label conjugatedto this antibody. Where the antibody is unlabeled, a second labeledantibody may be employed, e.g., which is specific for the isotype of theanti-marker polypeptide antibody. Examples of labels which may beemployed include radionuclides, fluorescers, chemiluminescers, enzymesand the like.

[0288] Where enzymes are employed, the substrate for the enzyme may beadded to the samples to provide a colored or fluorescent product.Examples of suitable enzymes for use in conjugates include horseradishperoxidase, alkaline phosphatase, malate dehydrogenase and the like.Where not commercially available, such antibody-enzyme conjugates arereadily produced by techniques known to those skilled in the art.

[0289] In one embodiment, the assay is performed as a dot blot assay.The dot blot assay finds particular application where tissue samples areemployed as it allows determination of the average amount of the markerpolypeptide associated with a single cell by correlating the amount ofmarker polypeptide in a cell-free extract produced from a predeterminednumber of cells.

[0290] It is well established in the cancer literature that tumor cellsof the same type (e.g., breast and/or colon tumor cells) may not showuniformly increased expression of individual oncogenes or uniformlydecreased expression of individual tumor suppressor genes. There mayalso be varying levels of expression of a given marker gene even betweencells of a given type of cancer, further emphasizing the need forreliance on a battery of tests rather than a single test. Accordingly,in one aspect, the invention provides for a battery of tests utilizing anumber of probes of the invention, in order to improve the reliabilityand/or accuracy of the diagnostic test.

[0291] In one embodiment, the present invention also provides a methodwherein nucleic acid probes are immobilized on a DNA chip in anorganized array. Oligonucleotides can be bound to a solid support by avariety of processes, including lithography. For example a chip can holdup to 250,000 oligonucleotides (GeneChip, Affymetrix). These nucleicacid probes comprise a nucleotide sequence at least about 12 nucleotidesin length, preferably at least about 15 nucleotides, more preferably atleast about 25 nucleotides, and most preferably at least about 40nucleotides, and up to all or nearly all of a sequence which iscomplementary to a portion of the coding sequence of a marker nucleicacid sequence represented by SEQ ID Nos:1-544 and is differentiallyexpressed in tumor cells, such as colon cancer cells. The presentinvention provides significant advantages over the available tests forvarious cancers, such as colon cancer, because it increases thereliability of the test by providing an array of nucleic acid markers ona single chip.

[0292] The method includes obtaining a biopsy, which is optionallyfractionated by cryostat sectioning to enrich tumor cells to about 80%of the total cell population. The DNA or RNA is then extracted,amplified, and analyzed with a DNA chip to determine the presence ofabsence of the marker nucleic acid sequences.

[0293] In one embodiment, the nucleic acid probes are spotted onto asubstrate in a two-dimensional matrix or array. Samples of nucleic acidscan be labeled and then hybridized to the probes. Double-strandednucleic acids, comprising the labeled sample nucleic acids bound toprobe nucleic acids, can be detected once the unbound portion of thesample is washed away.

[0294] The probe nucleic acids can be spotted on substrates includingglass, nitrocellulose, etc. The probes can be bound to the substrate byeither covalent bonds or by non-specific interactions, such ashydrophobic interactions. The sample nucleic acids can be labeled usingradioactive labels, fluorophores, chromophores, etc.

[0295] Techniques for constructing arrays and methods of using thesearrays are described in EP No. 0 799 897; PCT No. WO 97/29212; PCT No.WO 97/27317; EP No. 0 785 280; PCT No. WO 97/02357; U.S. Pat. No.5,593,839; U.S. Pat. No. 5,578,832; EP No. 0 728 520; U.S. Pat. No.5,599,695; EP No. 0 721 016; U.S. Pat. No. 5,556,752; PCT No. WO95/22058; and U.S. Pat. No. 5,631,734.

[0296] Further, arrays can be used to examine differential expression ofgenes and can be used to determine gene function. For example, arrays ofthe instant nucleic acid sequences can be used to determine if any ofthe nucleic acid sequences are differentially expressed between normalcells and cancer cells, for example. High expression of a particularmessage in a cancer cell, which is not observed in a correspondingnormal cell, can indicate a cancer specific protein.

[0297] In yet another embodiment, the invention contemplates using apanel of antibodies which are generated against the marker polypeptidesof this invention, which polypeptides are encoded by SEQ ID Nos:1-544.Such a panel of antibodies may be used as a reliable diagnostic probefor colon cancer. The assay of the present invention comprisescontacting a biopsy sample containing cells, e.g., colon cells, with apanel of antibodies to one or more of the encoded products to determinethe presence or absence of the marker polypeptides.

[0298] The diagnostic methods of the subject invention may also beemployed as follow-up to treatment, e.g., quantitation of the level ofmarker polypeptides may be indicative of the effectiveness of current orpreviously employed cancer therapies as well as the effect of thesetherapies upon patient prognosis.

[0299] Accordingly, the present invention makes available diagnosticassays and reagents for detecting gain and/or loss of markerpolypeptides from a cell in order to aid in the diagnosis andphenotyping of proliferative disorders arising from, for example,tumorigenic transformation of cells.

[0300] The diagnostic assays described above can be adapted to be usedas prognostic assays, as well. Such an application takes advantage ofthe sensitivity of the assays of the invention to events which takeplace at characteristic stages in the progression of a tumor. Forexample, a given marker gene may be up- or downregulated at a very earlyis stage, perhaps before the cell is irreversibly committed todeveloping into a malignancy, while another marker gene may becharacteristically up or down regulated only at a much later stage. Sucha method could involve the steps of contacting the mRNA of a test cellwith a nucleic acid probe derived from a given marker nucleic acid whichis expressed at different characteristic levels in cancerous orprecancerous cells at different stages of tumor progression, anddetermining the approximate amount of hybridization of the probe to themRNA of the cell, such amount being an indication of the level ofexpression of the gene in the cell, and thus an indication of the stageof tumor progression of the cell; alternatively, the assay can becarried out with an antibody specific for the gene product of the givenmarker nucleic acid, contacted with the proteins of the test cell. Abattery of such tests will disclose not only the existence and locationof a tumor, but also will allow the clinician to select the mode oftreatment most appropriate for the tumor, and to predict the likelihoodof success of that treatment.

[0301] The methods of the invention can also be used to follow theclinical course of a tumor. For example, the assay of the invention canbe applied to a tissue sample from a patient; following treatment of thepatient for the cancer, another tissue sample is taken and the testrepeated. Successful treatment will result in either removal of allcells which demonstrate differential expression characteristic of thecancerous or precancerous cells, or a substantial increase in expressionof the gene in those cells, perhaps approaching or even surpassingnormal levels.

[0302] In yet another embodiment, the invention provides methods fordetermining whether a subject is at risk for developing a disease, suchas a predisposition to develop cancer, for example colon cancer,associated with an aberrant activity of any one of the polypeptidesencoded by nucleic acids of SEQ ID Nos:1-544, wherein the aberrantactivity of the polypeptide is characterized by detecting the presenceor absence of a genetic lesion characterized by at least one of (i) analteration affecting the integrity of a gene encoding a markerpolypeptides, or (ii) the mis-expression of the encoding nucleic acid.To illustrate, such genetic lesions can be detected by ascertaining theexistence of at least one of (i) a deletion of one or more nucleotidesfrom the nucleic acid sequence, (ii) an addition of one or morenucleotides to the nucleic acid sequence, (iii) a substitution of one ormore nucleotides of the nucleic acid sequence, (iv) a gross chromosomalrearrangement of the nucleic acid sequence, (v) a gross alteration inthe level of a messenger RNA transcript of the nucleic acid sequence,(vii) aberrant modification of the nucleic acid sequence, such as of themethylation pattern of the genomic DNA, (vii) the presence of a non-wildtype splicing pattern of a messenger RNA transcript of the gene, (viii)a non-wild type level of the marker polypeptide, (ix) allelic loss ofthe gene, and/or (x) inappropriate post-translational modification ofthe marker polypeptide.

[0303] The present invention provides assay techniques for detectinglesions in the encoding nucleic acid sequence. These methods include,but are not limited to, methods involving sequence analysis, Southernblot hybridization, restriction enzyme site mapping, and methodsinvolving detection of absence of nucleotide pairing between the nucleicacid to be analyzed and a probe.

[0304] Specific diseases or disorders, e.g., genetic diseases ordisorders, are associated with specific allelic variants of polymorphicregions of certain genes, which do not necessarily encode a mutatedprotein. Thus, the presence of a specific allelic variant of apolymorphic region of a gene in a subject can render the subjectsusceptible to developing a specific disease or disorder. Polymorphicregions in genes, can be identified, by determining the nucleotidesequence of genes in populations of individuals. If a polymorphic regionis identified, then the link with a specific disease can be determinedby studying specific populations of individuals, e.g., individuals whichdeveloped a specific disease, such as colon cancer. A polymorphic regioncan be located in any region of a gene, e.g., exons, in coding or noncoding regions of exons, introns, and promoter region.

[0305] In an exemplary embodiment, there is provided a nucleic acidcomposition comprising a nucleic acid probe including a region ofnucleotide sequence which is capable of hybridizing to a sense orantisense sequence of a gene or naturally occurring mutants thereof, or5′ or 3′ flanking sequences or intronic sequences naturally associatedwith the subject genes or naturally occurring mutants thereof. Thenucleic acid of a cell is rendered accessible for hybridization, theprobe is contacted with the nucleic acid of the sample, and thehybridization of the probe to the sample nucleic acid is detected. Suchtechniques can be used to detect lesions or allelic variants at eitherthe genomic or mRNA level, including deletions, substitutions, etc., aswell as to determine mRNA transcript levels.

[0306] A preferred detection method is allele specific hybridizationusing probes overlapping the mutation or polymorphic site and havingabout 5, 10, 20, 25, or 30 nucleotides around the mutation orpolymorphic region. In a preferred embodiment of the invention, severalprobes capable of hybridizing specifically to allelic variants areattached to a solid phase support, e.g., a “chip”. Mutation detectionanalysis using these chips comprising oligonucleotides, also termed “DNAprobe arrays” is described e.g., in Cronin et al. (1996) Human Mutation7:244. In one embodiment, a chip comprises all the allelic variants ofat least one polymorphic region of a gene. The solid phase support isthen contacted with a test nucleic acid and hybridization to thespecific probes is detected. Accordingly, the identity of numerousallelic variants of one or more genes can be identified in a simplehybridization experiment.

[0307] In certain embodiments, detection of the lesion comprisesutilizing the probe/primer in a polymerase chain reaction (PCR) (see,e.g. U.S. Pat. Nos. 4,683,195 and 4,683,202), such as anchor PCR or RACEPCR, or, alternatively, in a ligase chain reaction (LCR) (see, e.g.,Landegran et al. (1988) Science 241:1077-1080; and Nakazawa et al.(1994) PNAS 91:360-364), the latter of which can be particularly usefulfor detecting point mutations in the gene (see Abravaya et al. (1995)Nuc Acid Res 23:675-682). In a merely illustrative embodiment, themethod includes the steps of (i) collecting a sample of cells from apatient, (ii) isolating nucleic acid (e.g., genomic, mRNA or both) fromthe cells of the sample, (iii) contacting the nucleic acid sample withone or more primers which specifically hybridize to a nucleic acidsequence under conditions such that hybridization and amplification ofthe nucleic acid (if present) occurs, and (iv) detecting the presence orabsence of an amplification product, or detecting the size of theamplification product and comparing the length to a control sample. Itis anticipated that PCR and/or LCR may be desirable to use as apreliminary amplification step in conjunction with any of the techniquesused for detecting mutations described herein.

[0308] Alternative amplification methods include: self sustainedsequence replication (Guatelli, J. C. et al., 1990, Proc. Natl. Acad.Sci. USA 87:1874-1878), transcriptional amplification system (Kwoh, D.Y. et al., 1989, Proc. Natl. Acad. Sci. USA 86:1173-1177), Q-BetaReplicase (Lizardi, P. M. et al., 1988, Bio/Technology 6:1197), or anyother nucleic acid amplification method, followed by the detection ofthe amplified molecules using techniques well known to those of skill inthe art. These detection schemes are especially useful for the detectionof nucleic acid molecules if such molecules are present in very lownumbers.

[0309] In a preferred embodiment of the subject assay, mutations in, orallelic variants, of a gene from a sample cell are identified byalterations in restriction enzyme cleavage patterns. For example, sampleand control DNA is isolated, amplified (optionally), digested with oneor more restriction endonucleases, and fragment length sizes aredetermined by gel electrophoresis. Moreover, the use of sequencespecific ribozymes (see, for example, U.S. Pat. No. 5,498,531) can beused to score for the presence of specific mutations by development orloss of a ribozyme cleavage site.

[0310] Another aspect of the invention is directed to the identificationof agents capable of modulating the differentiation and proliferation ofcells characterized by aberrant proliferation. In this regard, theinvention provides assays for determining compounds that modulate theexpression of the marker nucleic acids (SEQ ID Nos:1-544) and/or alterfor example, inhibit the bioactivity of the encoded polypeptide.

[0311] Several in vivo methods can be used to identify compounds thatmodulate expression of the marker nucleic acids (SEQ ID Nos:1-544)and/or alter for example, inhibit the bioactivity of the encodedpolypeptide.

[0312] Drug screening is performed by adding a test compound to a sampleof cells, and monitoring the effect. A parallel sample which does notreceive the test compound is also monitored as a control. The treatedand untreated cells are then compared by any suitable phenotypiccriteria, including but not limited to microscopic analysis, viabilitytesting, ability to replicate, histological examination, the level of aparticular RNA or polypeptide associated with the cells, the level ofenzymatic activity expressed by the cells or cell lysates, and theability of the cells to interact with other cells or compounds.Differences between treated and untreated cells indicates effectsattributable to the test compound.

[0313] Desirable effects of a test compound include an effect on anyphenotype that was conferred by the cancer-associated marker nucleicacid sequence. Examples include a test compound that limits theoverabundance of mRNA, limits production of the encoded protein, orlimits the functional effect of the protein. The effect of the testcompound would be apparent when comparing results between treated anduntreated cells.

[0314] The invention thus also encompasses methods of screening foragents which inhibit expression of the nucleic acid markers (SEQ IDNos:1-544) in vitro, comprising exposing a cell or tissue in which themarker nucleic acid mRNA is detectable in cultured cells to an agent inorder to determine whether the agent is capable of inhibiting productionof the mRNA; and determining the level of mRNA in the exposed cells ortissue, wherein a decrease in the level of the mRNA after exposure ofthe cell line to the agent is indicative of inhibition of the markernucleic acid mRNA production.

[0315] Alternatively, the screening method may include in vitroscreening of a cell or tissue in which marker protein is detectable incultured cells to an agent suspected of inhibiting production of themarker protein; and determining the level of the marker protein in thecells or tissue, wherein a decrease in the level of marker protein afterexposure of the cells or tissue to the agent is indicative of inhibitionof marker protein production.

[0316] The invention also encompasses in vivo methods of screening foragents which inhibit expression of the marker nucleic acids, comprisingexposing a mammal having tumor cells in which marker mRNA or protein isdetectable to an agent suspected of inhibiting production of marker mRNAor protein; and determining the level of marker mRNA or protein in tumorcells of the exposed mammal. A decrease in the level of marker mRNA orprotein after exposure of the mammal to the agent is indicative ofinhibition of marker nucleic acid expression.

[0317] Accordingly, the invention provides a method comprisingincubating a cell expressing the marker nucleic acids (SEQ ID Nos:1-544)with a test compound and measuring the mRNA or protein level. Theinvention further provides a method for quantitatively determining thelevel of expression of the marker nucleic acids in a cell population,and a method for determining whether an agent is capable of increasingor decreasing the level of expression of the marker nucleic acids in acell population. The method for determining whether an agent is capableof increasing or decreasing the level of expression of the markernucleic acids in a cell population comprises the steps of (a) preparingcell extracts from control and agent-treated cell populations, (b)isolating the marker polypeptides from the cell extracts, (c)quantifying (e.g., in parallel) the amount of an immunocomplex formedbetween the marker polypeptide and an antibody specific to saidpolypeptide. The marker polypeptides of this invention may also bequantified by assaying for its bioactivity. Agents that induce increasedthe marker nucleic acid expression may be identified by their ability toincrease the amount of immunocomplex formed in the treated cell ascompared with the amount of the immunocomplex formed in the controlcell. In a similar manner, agents that decrease expression of the markernucleic acid may be identified by their ability to decrease the amountof the immunocomplex formed in the treated cell extract as compared tothe control cell.

[0318] mRNA levels can be determined by Northern blot hybridization.mRNA levels can also be determined by methods involving PCR. Othersensitive methods for measuring mRNA, which can be used in highthroughput assays, e.g., a method using a DELFIA endpoint detection andquantification method, are described, e.g., in Webb and Hurskainen(1996) Journal of Biomolecular Screening 1:119. Marker protein levelscan be determined by immunoprecipitations or immunohistochemistry usingan antibody that specifically recognizes the protein product encoded bySEQ ID Nos:1-544.

[0319] Agents that are identified as active in the drug screening assayare candidates to be tested for their capacity to block cellproliferation activity. These agents would be useful for treating adisorder involving aberrant growth of cells, especially colon cells.

[0320] A variety of assay formats will suffice and, in light of thepresent disclosure, those not expressly described herein willnevertheless be comprehended by one of ordinary skill in the art. Forinstance, the assay can be generated in many different formats, andinclude assays based on cell-free systems, e.g., purified proteins orcell lysates, as well as cell-based assays which utilize intact cells.

[0321] In many drug screening programs which test libraries of compoundsand natural extracts, high throughput assays are desirable in order tomaximize the number of compounds surveyed in a given period of time.Assays of the present invention which are performed in cell-freesystems, such as may be derived with purified or semi-purified proteinsor with lysates, are often preferred as “primary” screens in that theycan be generated to permit rapid development and relatively easydetection of an alteration in a molecular target which is mediated by atest compound. Moreover, the effects of cellular toxicity and/orbioavailability of the test compound can be generally ignored in the invitro system, the assay instead being focused primarily on the effect ofthe drug on the molecular target as may be manifest in an alteration ofbinding affinity with other proteins or changes in enzymatic propertiesof the molecular target.

[0322] A. Use of Nucleic Acids as Probes in Mapping and in TissueProfiling Probes

[0323] Polynucleotide probes as described above, e.g., comprising atleast 12 contiguous nucleotides selected from the nucleotide sequence ofan nucleic acid as shown in SEQ ID Nos. 1-544, preferably SEQ ID Nos.1-168, even more preferably SEQ ID Nos. 1-35, or a sequencecomplementary thereto, are used for a variety of purposes, includingidentification of human chromosomes and determining transcriptionlevels. Additional disclosure about preferred regions of the nucleicacid sequences is found in the accompanying tables.

[0324] The nucleotide probes are labeled, for example, with aradioactive, fluorescent, biotinylated, or chemiluminescent label, anddetected by well known methods appropriate for the particular labelselected. Protocols for hybridizing nucleotide probes to preparations ofmetaphase chromosomes are also well known in the art. A nucleotide probewill hybridize specifically to nucleotide sequences in the chromosomepreparations which are complementary to the nucleotide sequence of theprobe. A probe that hybridizes specifically to a nucleic acid shouldprovide a detection signal at least 5-, 10-, or 20-fold higher than thebackground hybridization provided with other unrelated sequences.

[0325] In a non-limiting example, commercial programs are available foridentifying regions of chromosomes commonly associated with disease,such as cancer. Nucleic acids of the invention can be used to probethese regions. For example, if, through profile searching, a nucleicacid is identified as corresponding to a gene encoding a kinase, itsability to bind to a cancer-related chromosomal region will suggest itsrole as a kinase in one or more stages of tumor cell development/growth.Although some experimentation would be required to elucidate the role,the nucleic acid constitutes a new material for isolating a specificprotein that has potential for developing a cancer diagnostic ortherapeutic.

[0326] Nucleotide probes are used to detect expression of a genecorresponding to the nucleic acid. For example, in Northern blots, mRNAis separated electrophoretically and contacted with a probe. A probe isdetected as hybridizing to an mRNA species of a particular size. Theamount of hybridization is quantitated to determine relative amounts ofexpression, for example under a particular condition. Probes are alsoused to detect products of amplification by polymerase chain reaction.The products of the reaction are hybridized to the probe and hybrids aredetected. Probes are used for in situ hybridization to cells to detectexpression. Probes can also be used in vivo for diagnostic detection ofhybridizing sequences. Probes are typically labeled with a radioactiveisotope. Other types of detectable labels may be used such aschromophores, fluorophores, and enzymes.

[0327] Expression of specific mRNA can vary in different cell types andcan be tissue specific. This variation of mRNA levels in different celltypes can be exploited with nucleic acid probe assays to determinetissue types. For example, PCR, branched DNA probe assays, or blottingtechniques utilizing nucleic acid probes substantially identical orcomplementary to nucleic acids of SEQ ID Nos. 1-544, preferably SEQ IDNos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequencecomplementary thereto, can determine the presence or absence of targetcDNA or mRNA.

[0328] Examples of a nucleotide hybridization assay are described inUrdea et al., PCT WO92/02526 and Urdea et al., U.S. Pat. No. 5,124,246,both incorporated herein by reference. The references describe anexample of a sandwich nucleotide hybridization assay.

[0329] Alternatively, the Polymerase Chain Reaction (PCR) is anothermeans for detecting small amounts of target nucleic acids, as describedin Mullis et al., Meth. Enzymol. (1987) 155:335-350; U.S. Pat. No.4,683,195; and U.S. Pat. No. 4,683,202, all incorporated herein byreference. Two primer polynucleotides nucleotides hybridize with thetarget nucleic acids and are used to prime the reaction. The primers maybe composed of sequence within or 3′ and 5′ to the polynucleotides ofthe Sequence Listing. Alternatively, if the primers are 3′ and 5′ tothese polynucleotides, they need not hybridize to them or thecomplements. A thermostable polymerase creates copies of target nucleicacids from the primers using the original target nucleic acids as atemplate. After a large amount of target nucleic acids is generated bythe polymerase, it is detected by methods such as Southern blots. Whenusing the Southern blot method, the labeled probe will hybridize to apolynucleotide of the Sequence Listing or complement.

[0330] Furthermore, mRNA or cDNA can be detected by traditional blottingtechniques described in Sambrook et al., “Molecular Cloning: ALaboratory Manual” (New York, Cold Spring Harbor Laboratory, 1989). mRNAor cDNA generated from mRNA using a polymerase enzyme can be purifiedand separated using gel electrophoresis. The nucleic acids on the gelare then blotted onto a solid support, such as nitrocellulose. The solidsupport is exposed to a labeled probe and then washed to remove anyunhybridized probe. Next, the duplexes containing the labeled probe aredetected. Typically, the probe is labeled with radioactivity.

Mapping

[0331] Nucleic acids of the present invention are used to identify achromosome on which the corresponding gene resides. Using fluorescencein situ hybridization (FISH) on normal metaphase spreads, comparativegenomic hybridization allows total genome assessment of changes inrelative copy number of DNA sequences. See Schwartz and Samad, CurrentOpinions in Biotechnology (1994) 8:70-74; Kallioniemi et al., Seminarsin Cancer Biology (1993) 4:41-46; Valdes and Tagle, Methods in MolecularBiology (1997) 68:1, Boultwood, ed., Human Press, Totowa, N.J.

[0332] Preparations of human metaphase chromosomes are prepared usingstandard cytogenetic techniques from human primary tissues or celllines. Nucleotide probes comprising at least 12 contiguous nucleotidesselected from the nucleotide sequence of SEQ ID Nos. 1-544, preferablySEQ ID Nos. 1-168, even more preferably SEQ ID Nos. 1-35, or a sequencecomplementary thereto, are used to identify the correspondingchromosome. The nucleotide probes are labeled, for example, with aradioactive, fluorescent, biotinylated, or chemiluminescent label, anddetected by well known methods appropriate for the particular labelselected. Protocols for hybridizing nucleotide probes to preparations ofmetaphase chromosomes are also well known in the art. A nucleotide probewill hybridize specifically to nucleotide sequences in the chromosomepreparations that are complementary to the nucleotide sequence of theprobe. A probe that hybridizes specifically to a target gene provides adetection signal at least 5-, 10-, or 20-fold higher than the backgroundhybridization provided with unrelated coding sequences.

[0333] Nucleic acids are mapped to particular chromosomes using, forexample, radiation hybrids or chromosome-specific hybrid panels. SeeLeach et al., Advances in Genetics, (1995) 33:63-99; Walter et al.,Nature Genetics (1994) 7:22-28; Walter and Goodfellow, Trends inGenetics (1992) 9:352. Panels for radiation hybrid mapping are availablefrom Research Genentics, Inc., Huntsville, Ala., USA. Databases formarkers using various panels are available via the world wide web athttp:/F/shgc-www.stanford.edu; and other locations. The statisticalprogram RHMAP can be used to construct a map based on the data fromradiation hybridization with a measure of the relative likelihood of oneorder versus another. RHMAP is available via the world wide web athttp://www.sph.umich.edu/group/statgen/software.

[0334] Such mapping can be useful in identifying the function of thetarget gene by its proximity to other genes with known function.Function can also be assigned to the target gene when particularsyndromes or diseases map to the same chromosome.

Tissue Profiling

[0335] The nucleic acids of the present invention can be used todetermine the tissue type from which a given sample is derived. Forexample, a metastatic lesion is identified by its developmental organ ortissue source by identifying the expression of a particular marker ofthat organ or tissue. If a nucleic acid is expressed only in a specifictissue type, and a metastatic lesion is found to express that nucleicacid, then the developmental source of the lesion has been identified.Expression of a particular nucleic acid is assayed by detection ofeither the corresponding mRNA or the protein product. Immunologicalmethods, such as antibody staining, are used to detect a particularprotein product. Hybridization methods may be used to detect particularmRNA species, including but not limited to in situ hybridization andNorthern blotting.

Use of Polymorphisms

[0336] A nucleic acid will be useful in forensics, genetic analysis,mapping, and diagnostic applications if the corresponding region of agene is polymorphic in the human population. A particular polymorphicform of the nucleic acid may be used to either identify a sample asderiving from a suspect or rule out the possibility that the samplederives from the suspect. Any means for detecting a polymorphism in agene are used, including but not limited to electrophoresis of proteinpolymorphic variants, differential sensitivity to restriction enzymecleavage, and hybridization to an allele-specific probe.

[0337] B. Use of Nucleic Acids and Encoded Polypeptides to RaiseAntibodies

[0338] Expression products of a nucleic acid, the corresponding mRNA orcDNA, or the corresponding complete gene are prepared and used forraising antibodies for experimental, diagnostic, and therapeuticpurposes. For nucleic acids to which a corresponding gene has not beenassigned, this provides an additional method of identifying thecorresponding gene. The nucleic acid or related cDNA is expressed asdescribed above, and antibodies are prepared. These antibodies arespecific to an epitope on the encoded polypeptide, and can precipitateor bind to the corresponding native protein in a cell or tissuepreparation or in a cell-free extract of an in vitro expression system.

[0339] Immunogens for raising antibodies are prepared by mixing thepolypeptides encoded by the nucleic acids of the present invention withadjuvants. Alternatively, polypeptides are made as fusion proteins tolarger immunogenic proteins. Polypeptides are also covalently linked toother larger immunogenic proteins, such as keyhole limpet hemocyanin.Immunogens are typically administered intradermally, subcutaneously, orintramuscularly. Immunogens are administered to experimental animalssuch as rabbits, sheep, and mice, to generate antibodies. Optionally,the animal spleen cells are isolated and fused with myeloma cells toform hybridomas which secrete monoclonal antibodies. Such methods arewell known in the art. According to another method known in the art, thenucleic acid is administered directly, such as by intramuscularinjection, and expressed in vivo. The expressed protein generates avariety of protein-specific immune responses, including production ofantibodies, comparable to administration of the protein.

[0340] Preparations of polyclonal and monoclonal antibodies specific fornucleic acid-encoded proteins and polypeptides are made using standardmethods known in the art. The antibodies specifically bind to epitopespresent in the polypeptides encoded by a nucleic acid of SEQ ID Nos.1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ ID Nos.1-35, or a sequence complementary thereto. In another embodiment, theantibodies specifically bind to epitopes present in a polypeptideencoded by SEQ ID Nos. 1-544. Typically, at least about 6, 8, 10, or 12contiguous amino acids are required to form an epitope. However,epitopes which involve non-contiguous amino acids may require more, forexample, at least about 15, 25, or 50 amino acids. A short sequence of anucleic acid may then be unsuitable for use as an epitope to raiseantibodies for identifying the corresponding novel protein, because ofthe potential for cross-reactivity with a known protein. However, theantibodies may be useful for other purposes, particularly if theyidentify common structural features of a known protein and a novelpolypeptide encoded by a nucleic acid of the invention.

[0341] Antibodies that specifically bind to human nucleic acid-encodedpolypeptides should provide a detection signal at least about 5-, 10-,or 20-fold higher than a detection signal provided with other proteinswhen used in Western blots or other immunochemical assays. Preferably,antibodies that specifically bind nucleic acid T-encoded polypeptides donot detect other proteins in immunochemical assays and canimmunoprecipitate nucleic acid-encoded proteins from solution.

[0342] To test for the presence of serum antibodies to the nucleicacid-encoded polypeptide in a human population, human antibodies arepurified by methods well known in the art. Preferably, the antibodiesare affinity purified by passing antiserum over a column to which anucleic acid-encoded protein, polypeptide, or fusion protein is bound.The bound antibodies can then be eluted from the column, for exampleusing a buffer with a high salt concentration.

[0343] In addition to the antibodies discussed above, geneticallyengineered antibody derivatives are made, such as single chainantibodies.

[0344] Antibodies may be made by using standard protocols known in theart (See, for example, Antibodies: A Laboratory Manual ed. by Harlow andLane (Cold Spring Harbor Press: 1988)). A mammal, such as a mouse,hamster, or rabbit can be immunized with an immunogenic form of thepeptide (e.g., a mammalian polypeptide or an antigenic fragment which iscapable of eliciting an antibody response, or a fusion protein asdescribed above).

[0345] In one aspect, this invention includes monoclonal antibodies thatshow a subject polypeptide is highly expressed in colorectal tissue ortumor tissue, especially colon cancer tissue or colon cancer-derivedcell lines. Therefore, in one embodiment, this invention provides adiagnostic tool for the analysis of expression of a subject polypeptidein general, and in particular, as a diagnostic for colon cancer.

[0346] Techniques for conferring immunogenicity on a protein or peptideinclude conjugation to carriers or other techniques well known in theart. An immunogenic portion of a protein can be administered in thepresence of adjuvant. The progress of immunization can be monitored bydetection of antibody titers in plasma or serum. Standard ELISA or otherimmunoassays can be used with the immunogen as antigen to assess thelevels of antibodies. In a preferred embodiment, the subject antibodiesare immunospecific for antigenic determinants of a protein of a mammal,e.g., antigenic determinants of a protein encoded by one of SEQ ID Nos.1-544 or closely related homologs (e.g., at least 90% identical, andmore preferably at least 95% identical).

[0347] Following immunization of an animal with an antigenic preparationof a polypeptide, antisera can be obtained and, if desired, polyclonalantibodies isolated from the serum. To produce monoclonal antibodies,antibody-producing cells (lymphocytes) can be harvested from animmunized animal and fused by standard somatic cell fusion procedureswith immortalizing cells such as myeloma cells to yield hybridoma cells.Such techniques are well known in the art, and include, for example, thehybridoma technique (originally developed by Kohler and Milstein, (1975)Nature, 256: 495-497), the human B cell hybridoma technique (Kozbar etal., (1983) Immunology Today, 4: 72), and the EBV-hybridoma technique toproduce human monoclonal antibodies (Cole et al., (1985) MonoclonalAntibodies and Cancer Therapy, Alan R. Liss, Inc. pp. 77-96). Hybridomacells can be screened immunochemically for production of antibodiesspecifically reactive with a polypeptide of the present invention andmonoclonal antibodies isolated from a culture comprising such hybridomacells.

[0348] The term antibody as used herein is intended to include fragmentsthereof which are also specifically reactive with one of the subjectpolypeptides. Antibodies can be fragmented using conventional techniquesand the fragments screened for utility in the same manner as describedabove for whole antibodies. For example, F(ab)₂ fragments can begenerated by treating antibody with pepsin. The resulting F(ab)₂fragment can be treated to reduce disulfide bridges to produce Fabfragments. The antibody of the present invention is further intended toinclude bispecific, single-chain, and chimeric and humanized moleculeshaving affinity for a polypeptide conferred by at least one CDR regionof the antibody. In preferred embodiments, the antibodies, the antibodyfurther comprises a label attached thereto and able to be detected,(e.g., the label can be a radioisotope, fluorescent compound,chemiluminescent compound, enzyme, or enzyme co-factor).

[0349] Antibodies can be used, e.g., to monitor protein levels in anindividual for determining, e.g., whether a subject has a disease orcondition, such as colon cancer, associated with an aberrant proteinlevel, or allowing determination of the efficacy of a given treatmentregimen for an individual afflicted with such a disorder. The level ofpolypeptides may be measured from cells in bodily fluid, such as inblood samples.

[0350] Another application of antibodies of the present invention is inthe immunological screening of cDNA libraries constructed in expressionvectors such as gt11, gt18-23, ZAP, and ORF8. Messenger libraries ofthis type, having coding sequences inserted in the correct reading frameand orientation, can produce fusion proteins. For instance, gt11 willproduce fusion proteins whose amino termini consist of β-galactosidaseamino acid sequences and whose carboxyl termini consist of a foreignpolypeptide. Antigenic epitopes of a protein, e.g., other orthologs of aparticular protein or other paralogs from the same species, can then bedetected with antibodies, as, for example, reacting nitrocellulosefilters lifted from infected plates with antibodies. Positive phagedetected by this assay can then be isolated from the infected plate.Thus, the presence of homologs can be detected and cloned from otheranimals, as can alternate isoforms (including splicing variants) fromhumans.

[0351] In another embodiment, a panel of monoclonal antibodies may beused, wherein each of the epitope's involved functions are representedby a monoclonal antibody. Loss or perturbation of binding of amonoclonal antibody in the panel would be indicative of a mutationalattention of the protein and thus of the corresponding gene.

[0352] C. Differential Expression

[0353] The present invention also provides a method to identify abnormalor diseased tissue in a human. For nucleic acids corresponding toprofiles of protein families as described above, the choice of tissuemay be dictated by the putative biological function. The expression of agene corresponding to a specific nucleic acid is compared between afirst tissue that is suspected of being diseased and a second, normaltissue of the human. The normal tissue is any tissue of the human,especially those that express the target gene including, but not limitedto, brain, thymus, testis, heart, prostate, placenta, spleen, smallintestine, skeletal muscle, pancreas, and the mucosal lining of thecolon.

[0354] The tissue suspected of being abnormal or diseased can be derivedfrom a different tissue type of the human, but preferably it is derivedfrom the same tissue type; for example an intestinal polyp or otherabnormal growth should be compared with normal intestinal tissue. Adifference between the target gene, mRNA, or protein in the two tissueswhich are compared, for example in molecular weight, amino acid ornucleotide sequence, or relative abundance, indicates a change in thegene, or a gene which regulates it, in the tissue of the human that wassuspected of being diseased.

[0355] The target genes in the two tissues are compared by any meansknown in the art. For example, the two genes are sequenced, and thesequence of the gene in the tissue suspected of being diseased iscompared with the gene sequence in the normal tissue. The target genes,or portions thereof, in the two tissues are amplified, for example usingnucleotide primers based on the nucleotide sequence shown in theSequence Listing, using the polymerase chain reaction. The amplifiedgenes or portions of genes are hybridized to nucleotide probes selectedfrom a corresponding nucleotide sequence shown SEQ ID No. 1-544. Adifference in the nucleotide sequence of the target gene in the tissuesuspected of being diseased compared with the normal nucleotide sequencesuggests a role of the nucleic acid-encoded proteins in the disease, andprovides a lead for preparing a therapeutic agent. The nucleotide probesare labeled by a variety of methods, such as radiolabeling,biotinylation, or labeling with fluorescent or chemiluminescent tags,and detected by standard methods known in the art.

[0356] Alternatively, target mRNA in the two tissues is compared.PolyA⁺RNA is isolated from the two tissues as is known in the art. Forexample, one of skill in the art can readily determine differences inthe size or amount of target mRNA transcripts between the two tissuesusing Northern blots and nuclcotide probes selected from the nucleotidesequence shown in the Sequence Listing. Increased or decreasedexpression of a target mRNA in a tissue sample suspected of beingdiseased, compared with the expression of the same target mRNA in anormal tissue, suggests that the expressed protein has a role in thedisease, and also provides a lead for preparing a therapeutic agent.

[0357] Any method for analyzing proteins is used to compare two nucleicacid-encoded proteins from matched samples. The sizes of the proteins inthe two tissues are compared, for example, using antibodies of thepresent invention to detect nucleic acid-encoded proteins in Westernblots of protein extracts from the two tissues. Other changes, such asexpression levels and subcellular localization, can also be detectedimmunologically, using antibodies to the corresponding protein. A higheror lower level of nucleic acid-encoded protein expression in a tissuesuspected of being diseased, compared with the same nucleic acid-encodedprotein expression level in a normal tissue, is indicative that theexpressed protein has a role in the disease, and provides another leadfor preparing a therapeutic agent.

[0358] Similarly, comparison of gene sequences or of gene expressionproducts, e.g., mRNA and protein, between a human tissue that issuspected of being diseased and a normal tissue of a human, are used tofollow disease progression or remission in the human. Such comparisonsof genes, mRNA, or protein are made as described above.

[0359] For example, increased or decreased expression of the target genein the tissue suspected of being neoplastic can indicate the presence ofneoplastic cells in the tissue. The degree of increased expression ofthe target gene in the neoplastic tissue relative to expression of thegene in normal tissue, or differences in the amount of increasedexpression of the target gene in the neoplastic tissue over time, isused to assess the progression of the neoplasia in that tissue or tomonitor the response of the neoplastic tissue to a therapeutic protocolover time.

[0360] The expression pattern of any two cell types can be compared,such as low and high metastatic tumor cell lines, or cells from tissuewhich have and have not been exposed to a therapeutic agent. A geneticpredisposition to disease in a human is detected by comparing an targetgene, mRNA, or protein in a fetal tissue with a normal target gene,mRNA, or protein. Fetal tissues that are used for this purpose include,but are not limited to, amniotic fluid, chorionic villi, blood, and theblastomere of an in vitro-fertilized embryo. The comparable normaltarget gene is obtained from any tissue. The mRNA or protein is obtainedfrom a normal tissue of a human in which the target gene is expressed.Differences such as alterations in the nucleotide sequence or size ofthe fetal target gene or mRNA, or alterations in the molecular weight,amino acid sequence, or relative abundance of fetal target protein, canindicate a germline mutation in the target gene of the fetus, whichindicates a genetic predisposition to disease.

[0361] D. Use of Nucleic Acids and Encoded Polypeptides to Screen forPeptide Analogs and Antagonists

[0362] Polypeptides encoded by the instant nucleic acids, e.g., SEQ IDNos. 1-544, preferably SEQ ID Nos. 1-168, even more preferably SEQ IDNos. 1-35, or a sequence complementary thereto, and corresponding fulllength genes can be used to screen peptide libraries to identify bindingpartners, such as receptors, from among the encoded polypeptides.

[0363] A library of peptides may be synthesized following the methodsdisclosed in U.S. Pat. No. 5,010,175, and in PCT WO 91/17823. Asdescribed below in brief, one prepares a mixture of peptides, which isthen screened to identify the peptides exhibiting the desired signaltransduction and receptor binding activity. In the '175 method, asuitable peptide synthesis support (e.g., a resin) is coupled to amixture of appropriately protected, activated amino acids. Theconcentration of each amino acid in the reaction mixture is balanced oradjusted in inverse proportion to its coupling reaction rate so that theproduct is an equimolar mixture of amino acids coupled to the startingresin. The bound amino acids are then deprotected, and reacted withanother balanced amino acid mixture to form an equimolar mixture of allpossible dipeptides. This process is repeated until a mixture ofpeptides of the desired length (e.g., hexamers) is formed. Note that oneneed not include all amino acids in each step: one may include only oneor two amino acids in some steps (e.g., where it is known that aparticular amino acid is essential in a given position), thus reducingthe complexity of the mixture. After the synthesis of the peptidelibrary is completed, the mixture of peptides is screened for binding tothe selected polypeptide. The peptides are then tested for their abilityto inhibit or enhance activity. Peptides exhibiting the desired activityare then isolated and sequenced.

[0364] The method described in WO 91/17823 is similar. However, insteadof reacting the synthesis resin with a mixture of activated amino acids,the resin is divided into twenty equal portions (or into a number ofportions corresponding to the number of different amino acids to beadded in that step), and each amino acid is coupled individually to itsportion of resin. The resin portions are then combined, mixed, and againdivided into a number of equal portions for reaction with the secondamino acid. In this manner, each reaction may be easily driven tocompletion. Additionally, one may maintain separate “subpools” bytreating portions in parallel, rather than combining all resins at eachstep. This simplifies the process of determining which peptides areresponsible for any observed receptor binding or signal transductionactivity.

[0365] In such cases, the subpools containing, e.g., 1-2,000 candidateseach are exposed to one or more polypeptides of the invention. Eachsubpool that produces a positive result is then resynthesized as a groupof smaller subpools (sub-subpools) containing, e.g., 20-100 candidates,and reassayed. Positive sub-subpools may be resynthesized as individualcompounds, and assayed finally to determine the peptides that exhibit ahigh binding constant. These peptides can be tested for their ability toinhibit or enhance the native activity. The methods described in WO91/7823 and U.S. Pat. No. 5,194,392 (herein incorporated by reference)enable the preparation of such pools and subpools by automatedtechniques in parallel, such that all synthesis and resynthesis may beperformed in a matter of days.

[0366] Peptide agonists or antagonists are screened using any availablemethod, such as signal transduction, antibody binding, receptor binding,mitogenic assays, chemotaxis assays, etc. The methods described hereinare presently preferred. The assay conditions ideally should resemblethe conditions under which the native activity is exhibited in vivo,that is, under physiologic pH, temperature, and ionic strength. Suitableagonists or antagonists will exhibit strong inhibition or enhancement ofthe native activity at concentrations that do not cause toxic sideeffects in the subject. Agonists or antagonists that compete for bindingto the native polypeptide may require concentrations equal to or greaterthan the native concentration, while inhibitors capable of bindingirreversibly to the polypeptide may be added in concentrations on theorder of the native concentration.

[0367] The end results of such screening and experimentation will be atleast one novel polypeptide binding partner, such as a receptor, encodedby a nucleic acid of the invention, and at least one peptide agonist orantagonist of the novel binding partner. Such agonists and antagonistscan be used to modulate, enhance, or inhibit receptor function in cellsto which the receptor is native, or in cells that possess the receptoras a result of genetic engineering. Further, if the novel receptorshares biologically important characteristics with a known receptor,information about agonist/antagonist binding may help in developingimproved agonists/antagonists of the known receptor.

[0368] E. Pharmaceutical Compositions and Therapeutic Uses

[0369] Pharmaceutical compositions can comprise polypeptides,antibodies, or polynucleotides of the claimed invention. Thepharmaceutical compositions will comprise a therapeutically effectiveamount of either polypeptides, antibodies, or polynucleotides of theclaimed invention.

[0370] The term “therapeutically effective amount” as used herein refersto an amount of a therapeutic agent to treat, ameliorate, or prevent adesired disease or condition, or to exhibit a detectable therapeutic orpreventative effect. The effect can be detected by, for example,chemical markers or antigen levels. Therapeutic effects also includereduction in physical symptoms, such as decreased body temperature. Theprecise effective amount for a subject will depend upon the subject'ssize and health, the nature and extent of the condition, and thetherapeutics or combination of therapeutics selected for administration.Thus, it is not useful to specify an exact effective amount in advance.However, the effective amount for a given situation can be determined byroutine experimentation and is within the judgment of the clinician.

[0371] For purposes of the present invention, an effective dose will befrom about 0.01 mg/ kg to 50 mg/kg or 0.05 mg/kg to about 10 mg/kg ofthe DNA constructs in the individual to which it is administered.

[0372] A pharmaceutical composition can also contain a pharmaceuticallyacceptable carrier. The term “pharmaceutically acceptable carrier”refers to a carrier for administration of a therapeutic agent, such asantibodies or a polypeptide, genes, and other therapeutic agents. Theterm refers to any pharmaceutical carrier that does not itself inducethe production of antibodies harmful to the individual receiving thecomposition, and which may be administered without undue toxicity.Suitable carriers may be large, slowly metabolized macromolecules suchas proteins, polysaccharides, polylactic acids, polyglycolic acids,polymeric amino acids, amino acid copolymers, and inactive virusparticles. Such carriers are well known to those of ordinary skill inthe art.

[0373] Pharmaceutically acceptable salts can be used therein, forexample, mineral acid salts such as hydrochlorides, hydrobromides,phosphates, sulfates, and the like; and the salts of organic acids suchas acetates, propionates, malonates, benzoates, and the like. A thoroughdiscussion of pharmaceutically acceptable excipients is available inRemington's Pharmaceutical Sciences (Mack Pub. Co., N.J. 1991).

[0374] Pharmaceutically acceptable carriers in therapeutic compositionsmay contain liquids such as water, saline, glycerol and ethanol.Additionally, auxiliary substances, such as wetting or emulsifyingagents, pH buffering substances, and the like, may be present in suchvehicles. Typically, the therapeutic compositions are prepared asinjectables, either as liquid solutions or suspensions; solid formssuitable for solution in, or suspension in, liquid vehicles prior toinjection may also be prepared. Liposomes are included within thedefinition of a pharmaceutically acceptable carrier.

Delivery Methods

[0375] Once formulated, the nucleic acid compositions of the inventioncan be (1) administered directly to the subject; (2) delivered ex vivo,to cells derived from the subject; or (3) delivered in vitro forexpression of recombinant proteins.

[0376] Direct delivery of the compositions will generally beaccomplished by injection, either subcutaneously, intraperitoneally,intravenously or intramuscularly, or delivered to the interstitial spaceof a tissue. The compositions can also be administered into a tumor orlesion. Other modes of administration include oral and pulmonaryadministration, suppositories, and transdermal applications, needles,and gene guns or hyposprays. Dosage treatment may be a single doseschedule or a multiple dose schedule.

[0377] Methods for the ex vivo delivery and reimplantation oftransformed cells into a subject are known in the art and described ine.g., International Publication No. WO 93/14778. Examples of cellsuseful in ex vivo applications include, for example, stem cells,particularly hematopoetic, lymph cells, macrophages, dendritic cells, ortumor cells.

[0378] Generally, delivery of nucleic acids for both ex vivo and invitro applications can be accomplished by, for example, dextran-mediatedtransfection, calcium phosphate precipitation, polybrene mediatedtransfection, protoplast fusion, electroporation, encapsulation of thepolynucleotide(s) in liposomes, and direct microinjection of the DNAinto nuclei, all well known in the art.

[0379] Once a subject gene has been found to correlate with aproliferative disorder, such as neoplasia, dysplasia, and hyperplasia,the disorder may be amenable to treatment by administration of atherapeutic agent based on the nucleic acid or correspondingpolypeptide.

[0380] Preparation of antisense polypeptides is discussed above.Neoplasias that are treated with the antisense composition include, butare not limited to, cervical cancers, melanomas, colorectaladenocarcinomas, Wilms' tumor, retinoblastoma, sarcomas, myosarcomas,lung carcinomas, leukemias, such as chronic myelogenous leukemia,promyelocytic leukemia, monocytic leukemia, and myeloid leukemia, andlymphomas, such as histiocytic lymphoma. Proliferative disorders thatare treated with the therapeutic composition include disorders such asanhydric hereditary ectodermal dysplasia, congenital alveolar dysplasia,epithelial dysplasia of the cervix, fibrous dysplasia of bone, andmammary dysplasia. Hyperplasias, for example, endometrial, adrenal,breast, prostate; or thyroid hyperplasias or pseudoepitheliomatoushyperplasia of the skin, are treated with antisense therapeuticcompositions. Even in disorders in which mutations in the correspondinggene are not implicated, downregulation or inhibition of nucleicacid-related gene expression can have therapeutic application. Forexample, decreasing nucleic acid-related gene expression can help tosuppress tumors in which enhanced expression of the gene is implicated.

[0381] Both the dose of the antisense composition and the means ofadministration are determined based on the specific qualities of thetherapeutic composition, the condition, age, and weight of the patient,the progression of the disease, and other relevant factors.Administration of the therapeutic antisense agents of the inventionincludes local or systemic administration, including injection, oraladministration, particle gun or catheterized administration, and topicaladministration. Preferably, the therapeutic antisense compositioncontains an expression construct comprising a promoter and apolynucleotide segment of at least about 12, 22, 25, 30, or 35contiguous nucleotides of the antisense strand of a nucleic acid. Withinthe expression construct, the polynucleotide segment is locateddownstream from the promoter, and transcription of the polynucleotidesegment initiates at the promoter.

[0382] Various methods are used to administer the therapeuticcomposition directly to a specific site in the body. For example, asmall metastatic lesion is located and the therapeutic compositioninjected several times in several different locations within the body oftumor. Alternatively, arteries which serve a tumor are identified, andthe therapeutic composition injected into such an artery, in order todeliver the composition directly into the tumor. A tumor that has anecrotic center is aspirated and the composition injected directly intothe now empty center of the tumor. The antisense composition is directlyadministered to the surface of the tumor, for example, by topicalapplication of the composition. X-ray imaging is used to assist incertain of the above delivery methods.

[0383] Receptor-mediated targeted delivery of therapeutic compositionscontaining an antisense polynucleotide, subgenomic polynucleotides, orantibodies to specific tissues is also used. Receptor-mediated DNAdelivery techniques are described in, for example, Findeis et al.,Trends in Biotechnol. (1993) 11:202-205; Chiou et al., (1994) GeneTherapeutics: Methods And Applications Of Direct Gene Transfer (J. A.Wolff, ed.); Wu & Wu, J. Biol. Chem. (1988) 263:621-24; Wu et al., J.Biol. Chem. (1994) 269:542-46; Zenke et al., Proc. Natl. Acad. Sci.(USA) (1990) 87:3655-59; Wu et al., J. Biol. Chem. (1991) 266:338-42.Preferably, receptor-mediated targeted delivery of therapeuticcompositions containing antibodies of the invention is used to deliverthe antibodies to specific tissue.

[0384] Therapeutic compositions containing antisense subgenomicpolynucleotides are administered in a range of about 100 ng to about 200mg of DNA for local administration in a gene therapy protocol.Concentration ranges of about 500 ng to about 50 mg, about 1 mg to about2 mg, about 5 mg to about 500 mg, and about 20 mg to about 100 mg of DNAcan also be used during a gene therapy protocol. Factors such as methodof action and efficacy of transformation and expression areconsiderations which will affect the dosage required for ultimateefficacy of the antisense subgenomic nucleic acids. Where greaterexpression is desired over a larger area of tissue, larger amounts ofantisense subgenomic nucleic acids or the same amounts readministered ina successive protocol of administrations, or several administrations todifferent adjacent or close tissue portions of, for example, a tumorsite, may be required to effect a positive therapeutic outcome. In allcases, routine experimentation in clinical trials will determinespecific ranges for optimal therapeutic effect. A more completedescription of gene therapy vectors, especially retroviral vectors, iscontained in U.S. Ser. No. 08/869,309, which is expressly incorporatedherein, and in section F below.

[0385] For genes encoding polypeptides or proteins withanti-inflammatory activity, suitable use, doses, and administration aredescribed in U.S. Pat. No. 5,654,173, incorporated herein by reference.Therapeutic agents also include antibodies to proteins and polypeptidesencoded by the subject nucleic acids, as described in U.S. Pat. No.5,654,173.

[0386] F. Gene Therapy

[0387] The therapeutic nucleic acids of the present invention may beutilized in gene delivery vehicles. The gene delivery vehicle may be ofviral or non-viral origin (see generally, Jolly, Cancer Gene Therapy(1994) 1:51-64; Kimura, Human Gene Therpay (1994) 5:845-852; Connelly,Human Gene Therapy (1995) 1:185-193; and Kaplitt, Nature Genetics (1994)6:148-153). Gene therapy vehicles for delivery of constructs including acoding sequence of a therapeutic of the invention can be administeredeither locally or systemically. These constructs can utilize viral ornon-viral vector approaches. Expression of such coding sequences can beinduced using endogenous mammalian or heterologous promoters. Expressionof the coding sequence can be either constitutive or regulated.

[0388] The present invention can employ recombinant retroviruses whichare constructed to carry or express a selected nucleic acid molecule ofinterest. Retrovirus vectors that can be employed include thosedescribed in EP 0 415 731; WO 90/07936; WO 94/03622; WO 93/25698; WO93/25234; U.S. Pat. No. 5, 219,740; WO 93/11230; WO 93/10218; Vile andHart, Cancer Res. (1993) 53:3860-3864; Vile and Hart, Cancer Res. (1993)53:962-967; Ram et al., Cancer Res. (1993) 53:83-88; Takamiya et al., J.Neurosci. Res. (1992) 33:493-503; Baba et al., J. Neurosurg. (1993)79:729-735; U.S. Pat. No. 4,777,127; GB Patent No. 2,200,651; and EP 0345 242. Preferred recombinant retroviruses include those described inWO 91/02805.

[0389] Packaging cell lines suitable for use with the above-describedretroviral vector constructs may be readily prepared (see PCTpublications WO 95/30763 and WO 92/05266), and used to create producercell lines (also termed vector cell lines) for the production ofrecombinant vector particles. Within particularly preferred embodimentsof the invention, packaging cell lines are made from human (such asHT1080 cells) or mink parent cell lines, thereby allowing production ofrecombinant retroviruses that can survive inactivation in human serum.

[0390] The present invention also employs alphavirus-based vectors thatcan function as gene delivery vehicles. Such vectors can be constructedfrom a wide variety of alphaviruses, including, for example, Sindbisvirus vectors, Semliki forest virus (ATCC VR-67; ATCC VR-1247), RossRiver virus (ATCC VR-373; ATCC VR-1246) and Venezuelan equineencephalitis virus (ATCC VR-923; ATCC VR-1250; ATCC VR 1249; ATCCVR-532). Representative examples of such vector systems include thosedescribed in U.S. Pat. Nos. 5,091,309; 5,217,879; and 5,185,440; and PCTPublication Nos. WO 92/10578; WO 94/21792; WO 95/27069; WO 95/27044; andWO 95/07994.

[0391] Gene delivery vehicles of the present invention can also employparvovirus such as adeno-associated virus (AAV) vectors. Representativeexamples include the AAV vectors disclosed by Srivastava in WO 93/09239,Samulski et al., J. Vir. (1989) 63:3822-3828; Mendelson et al., Virol.(1988) 166:154-165; and Flotte et al., PNAS (1993) 90:10613-10617.

[0392] Representative examples of adenoviral vectors include thosedescribed by Berkner, Biotechniques (1988) 6:616-627; Rosenfeld et al.,Science (1991) 252:431-434; WO 93/19191; Kolls et al., PNAS (1994)91:215-219; Kass-Eisler et al., PNAS (1993) 90:11498-11502; Guzman etal., Circulation (1993) 88:2838-2848; Guzman et al., Cir. Res. (1993)73:1202-1207; Zabner et al., Cell (1993) 75:207-216; Li et al., Hum.Gene Ther. (1993) 4:403-409; Cailaud et al., Eur. J. Neurosci. (1993)5:1287-1291; Vincent et al., Nat. Genet. (1993) 5:130-134; Jaffe et al.,Nat. Genet. (1992) 1:372-378; and Levrero et al., Gene (1991)101:195-202. Exemplary adenoviral gene therapy vectors employable inthis invention also include those described in WO 94/12649, WO 93/03769;WO 93/19191; WO 94/28938; WO 95/11984 and WO 95/00655. Administration ofDNA linked to killed adenovirus as described in Curiel, Hum. Gene Ther.(1992) 3:147-154 may be employed.

[0393] Other gene delivery vehicles and methods may be employed,including polycationic condensed DNA linked or unlinked to killedadenovirus alone, for example Curiel, Hum. Gene Ther. (1992) 3:147-154;ligand linked DNA, for example see Wu, J. Biol. Chem. (1989)264:16985-16987; eukaryotic cell delivery vehicles cells, for examplesee U.S. Ser. No. 08/240,030, filed May 9, 1994, and U.S. Ser. No.08/404,796; deposition of photopolymerized hydrogel materials; hand-heldgene transfer particle gun, as described in U.S. Pat. No. 5,149,655;ionizing radiation as described in U.S. Pat. No. 5,206,152 and inWO92/11033; nucleic charge neutralization or fusion with cell membranes.Additional approaches are described in Philip, Mol. Cell Biol. (1994)14:2411-2418, and in Woffendin, Proc. Natl. Acad. Sci. (1994)91:1581-1585.

[0394] Naked DNA may also be employed. Exemplary naked DNA introductionmethods are described in WO 90/11092 and U.S. Pat. No. 5,580,859. Uptakeefficiency may be improved using biodegradable latex beads. DNA coatedlatex beads are efficiently transported into cells after endocytosisinitiation by the beads. The method may be improved further by treatmentof the beads to increase hydrophobicity and thereby facilitatedisruption of the endosome and release of the DNA into the cytoplasm.Liposomes that can act as gene delivery vehicles are described in U.S.Pat. No. 5,422,120, PCT Nos. WO 95/13796, WO 94/23697, and WO 91/14445,and EP No. 0 524 968.

[0395] Further non-viral delivery suitable for use includes mechanicaldelivery systems such as the approach described in Woffendin et al.,Proc. Natl. Acad. Sci. USA (1994) 91(24):11581-11585. Moreover, thecoding sequence and the product of expression of such can be deliveredthrough deposition of photopolymerized hydrogel materials. Otherconventional methods for gene delivery that can be used for delivery ofthe coding sequence include, for example, use of hand-held gene transferparticle gun, as described in U.S. Pat. No. 5,149,655; use of ionizingradiation for activating transferred gene, as described in U.S. Pat. No.5,206,152 and PCT No. WO 92/11033.

[0396] G. Transgenic Animals

[0397] One aspect of the present invention relates to transgenicnon-human animals having germline and/or somatic cells in which thebiological activity of one or more genes are altered by a chromosomallyincorporated transgene.

[0398] In a preferred embodiments, the transgene encodes a mutantprotein, such as dominant negative protein which antagonizes at least aportion of the biological function of a wild-type protein.

[0399] Yet another preferred transgenic animal includes a transgeneencoding an antisense transcript which, when transcribed from thetransgene, hybridizes with a gene or a mRNA transcript thereof, andinhibits expression of the gene.

[0400] In one embodiment, the present invention provides a desirednon-human animal or an animal (including human) cell which contains apredefined, specific and desired alteration rendering the non-humananimal or animal cell predisposed to cancer. Specifically, the inventionpertains to a genetically altered non-human animal (most preferably, amouse), or a cell (either non-human animal or human) in culture, that isdefective in at least one of two alleles of a tumor-suppressor gene. Theinactivation of at least one of these tumor suppressor alleles resultsin an animal with a higher susceptibility to tumor induction or otherproliferative or differentiative disorders, or disorders marked byaberrant signal transduction, e.g., from a cytokine or growth factor. Agenetically altered mouse of this type is able to serve as a usefulmodel for hereditary cancers and as a test animal for carcinogenstudies. The invention additionally pertains to the use of suchnon-human animals or animal cells, and their progeny in research andmedicine.

[0401] Furthermore, it is contemplated that cells of the transgenicanimals of the present invention can include other transgenes, e.g.,which alter the biological activity of a second tumor suppressor gene oran oncogene. For instance, the second transgene can functionally disruptthe biological activity of a second tumor suppressor gene, such as p53,p73, DCC, p21^(cip1), p27^(kip1), Rb, Mad or E2F. Alternatively, thesecond transgene can cause overexpression or loss of regulation of anoncogene, such as ras, myc, a cdc25 phosphatase, Bcl-2, Bcl-6, atransforming growth factor, neu, int-3, polyoma virus middle T antigen,SV40 large T antigen, a papillomaviral E6 protein, a papillomaviral E7protein, CDK4, or cyclin D1.

[0402] A preferred transgenic non-human animal of the present inventionhas germline and/or somatic cells in which one or more alleles of a geneare disrupted by a chromosomally incorporated transgene, wherein thetransgene includes a marker sequence providing a detectable signal foridentifying the presence of the transgene in cells of the transgenicanimal, and replaces at least a portion of the gene or is inserted intothe gene or disrupts expression of a wild-type protein.

[0403] Still another aspect of the present invention relates to methodsfor generating non-human animals and stem cells having a functionallydisrupted endogenous gene. In a preferred embodiment, the methodcomprises the steps of:

[0404] (i) constructing a transgene construct including (a) arecombination region having at least a portion of the gene, whichrecombination region directs recombination of the transgene with thegene, and (b) a marker sequence which provides a detectable signal foridentifying the presence of the transgene in a cell;

[0405] (ii) transferring the transgene into stem cells of a non-humananimal;

[0406] (iii) selecting stem cells having a correctly targeted homologousrecombination between the transgene and the gene;

[0407] (iv) transferring cells identified in step (iii) into a non-humanblastocyst and implanting the resulting chimeric blastocyst into anon-human female; and

[0408] (v) collecting offspring harboring an endogenous gene allelehaving the correctly targeted recombination.

[0409] Yet another aspect of the invention provides a method forevaluating the carcinogenic potential of an agent by (i) contacting atransgenic animal of the present invention with a test agent, and (ii)comparing the number of transformed cells in a sample from the treatedanimal with the number of transformed cells in a sample from anuntreated transgenic animal or transgenic animal treated with a controlagent. The difference in the number of transformed cells in the treatedanimal, relative to the number of transformed cells in the absence oftreatment with a control agent, indicates the carcinogenic potential ofthe test compound.

[0410] Another aspect of the invention provides a method of evaluatingan anti-proliferative activity of a test compound. In preferredembodiments, the method includes contacting a transgenic animal of thepresent invention, or a sample of cells from such animal, with a testagent, and determining the number of transformed cells in a specimenfrom the transgenic animal or in the sample of cells. A statisticallysignificant decrease in the number of transformed cells, relative to thenumber of transformed cells in the absence of the test agent, indicatesthe test compound is a potential anti-proliferative agent.

[0411] The practice of the present invention will employ, unlessotherwise indicated, conventional techniques of cell biology, cellculture, molecular biology, transgenic biology, microbiology,recombinant DNA, and immunology, which are within the skill of the art.Such techniques are explained fully in the literature. See, for example,Molecular Cloning A Laboratory Manual, 2nd Ed., ed. by Sambrook, Fritschand Maniatis (Cold Spring Harbor Laboratory Press:1989); DNA Cloning,Volumes I and II (D. N. Glover ed., 1985); Oligonucleotide Synthesis (M.J. Gait ed., 1984); Mullis et al. U.S. Pat. No. 4,683,195; Nucleic AcidHybridization (B. D. Hames & S. J. Higgins eds. 1984); Transcription AndTranslation (B. D. Hames & S. J. Higgins eds. 1984); Culture Of AnimalCells (R. I. Freshney, Alan R. Liss, Inc., 1987); Immobilized Cells AndEnzymes (IRL Press, 1986); B. Perbal, A Practical Guide To MolecularCloning (1984); the treatise, Methods In Enzymology (Academic Press,Inc., N.Y.); Gene Transfer Vectors For Mammalian Cells (J. H. Miller andM. P. Calos eds., 1987, Cold Spring Harbor Laboratory); Methods InEnzymology, Vols. 154 and 155 (Wu et al. eds.), Immunochemical MethodsIn Cell And Molecular Biology (Mayer and Walker, eds., Academic Press,London, 1987); Handbook Of Experimental Immunology, Volumes I-IV (D. M.Weir and C. C. Blackwell, eds., 1986); Manipulating the Mouse Embryo,(Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986).

[0412] As mentioned above, the sequences described herein are believedto have particular utility in regards to colon cancer. However, they mayalso be useful with other types of cancers and other disease states.

[0413] The present invention will now be illustrated by reference to thefollowing examples which set forth particularly advantageousembodiments. However, it should be noted that these embodiments areillustrative and are not to be construed as restricting the invention inany way.

XI. Examples

[0414] A. Identification of Differentially Expressed Sequences

Description of the Libraries

[0415] SEQ ID Nos:1-544 were derived from libraries designated as DE andPA as described below. The DE library is a normalized, colon cancerspecific, subtracted cDNA library. The DE library is specific forsequences expressed in colon cancer [proximal and distal Dukes' B,microsatellite instability negative (MSI-)] but not expressed in normaltissues, including normal colon tissue. The PA library is a normalized,colon specific, subtracted cDNA library. The PA library is specific forsequences expressed in normal colon tissue but not expressed in othernormal tissues.

Construction of a Colon Cancer Specific Library

[0416] A subtracted colon cancer specific library was made bysubtracting pooled proximal, stage B, MSI⁻ and distal, Stage B, MSI⁻tumor tissue cDNA against a combination of pooled driver normal cDNAmade from colon, peripheral blood leukocytes (PBL), liver, spleen, lung,kidney, heart, small intestine, skeletal muscle, and prostate tissuecDNAs. The following RNA samples were obtained from OrigeneTechnologies, Inc., Rockville, Md., and were used to synthesize thepooled driver cDNA: #HT-1015 normal colon total RNA, #HT-1005 livertotal RNA, #HT-1004 spleen total RNA, #HT-1009 lung total RNA, #HT-1003kidney total RNA, #HT-1006 peripheral blood leukocyte total RNA,#HT-prostate total RNA, #HM-1002 heart muscle poly A+ RNA, #HM-1007intestine poly A+ RNA, and #HM-1008 skeletal muscle poly A+ RNA.First-strand cDNA was prepared for each using 1 microgram of RNA. Abiased pool of first-strand cDNA was prepared containing 50% normalcolon first-strand cDNA reaction and 5.56% of each of the remainingtissue first-strand cDNA reactions by volume. Eight individualamplification reactions, each containing 1 microliter of the biasedfirst-strand cDNA reaction pool, were performed for 18 cycles. Thedouble stranded cDNA product from all eight amplification reactions werepooled and purified for subsequent use in subtractive hybridization. Thecolon cancer specific subtracted library was called DE and individualclones derived from this library were referred to with a number prefixedby DE.

[0417] Normalized subtracted DE colon cancer specific and pooled normalhuman tissue specific cDNA libraries (same as components of driver cDNAabove) were generated according published procedures (Daitchenko et al.,1996 PNAS 93:6025-6030, Gurskaya et al., 1996 Analytical Biochemistry240:90-97) using Clontech Laboratories, Inc., PCR-Select cDNAsubtraction kit, PT1117-1. A forty-five fold mass excess of driver cDNA(450 nanograms) was used for each subtraction experiment. Subtractivehybridization of tester with driver cDNAs was performed twice, each timefor about 8-12 hours. Subtracted cancer specific DE cDNA was ligatedinto the pCR2.1-TOPO plasmid vector (Invitrogen Corporation, CarlsbadCalif.) and chemically transformed into ultracompetent Epicurian E. coliXL10-Gold cells (Stratagene, La Jolla, Calif.). A reverse library wasalso constructed wherein the tester and driver samples were switched;this library was designated as MD.

Construction of a Normal Colon Specific Library

[0418] This normal colon tissue specific library was made using ClontechLaboratories Inc PCR-Select kit, K1804-1, following instructions fromthe users manual (PT1117-1).

[0419] Four, 100 μl, SMART PCR cDNA amplification reactions for eachnormal, non-cancerous, patient sample, were performed, starting with 1μl from their respective first strand cDNA reactions. Each sample wasamplified for only 18 cycles using the following PCR conditions; 95 C-10sec, 68 C 5 min. using a 9600 Perkin Elmer instrument. The following areBayer Diagnostic sample identification numbers for the cDNA samples thatwere amplified: NPB(−) 27347, NPB(−)27859, NPB(−)28147, NPB(−)28162,NDB(−)28800, NDB(−)29243, NDB(−)29244 and NDB(−)42472. These are normalcolon tissue samples obtained from the same patients providing theproximal stage B MSI—and distal stage B MSI-cancer samples, which wereused to prepare the DE library described above. Equal volumes of theeight normal colon cDNAs were pooled. A subtracted normal colon tissuespecific library was made by subtracting the normal colon cDNA poolagainst a combination of pooled driver normal cDNA made from peripheralblood leukocytes (PBL), liver, spleen, lung, kidney, heart, smallintestine, skeletal muscle, and prostate tissue cDNAs. The following arethe RNA samples that were used to synthesize the pooled driver cDNA:#HT-1005 liver total RNA, #HT-1004 spleen total RNA, #HT-1009 lung totalRNA, #HT-1003 kidney total RNA, #HT-1006 peripheral blood leukocytetotal RNA, #HT-prostate total RNA, #HM-1002 heart muscle poly A+ RNA,#HM-1007 intestine poly A+ RNA, and #HM-1008 skeletal muscle poly A+RNA. First-strand cDNA was prepared for each using 1 microgram of RNA. Apool of first strand cDNA reactions was then made consisting of equalvolumes of the nine driver tissue first-strand cDNA reactions. Eightindividual amplification reactions, each containing 1 microliter of thefirst-strand cDNA reaction pool, were performed for 18 cycles. Thedouble stranded cDNA product from all eight amplification reactions waspooled and purified for subsequent use in subtractive hybridization. Thenormal colon tissue specific subtracted library was called PA andindividual clones derived from this library were referred to with anumber prefixed by PA.

[0420] The normalized subtracted PA normal colon specific cDNA libraryand a subtracted normal human tissue specific cDNA library, consistingof the human tissues listed above were generated according publishedprocedures (Daitchenko et al., 1996 PNAS 93:6025-6030, Gurskaya et al.,1996 Analytical Biochemistry 240:90-97) using Clontech Laboratories,Inc., PCR-Select cDNA subtraction kit, PT1117-1. Library constructionand cloning were carried out as described above for the colon cancerspecific library. Out of the 1152 clones that were analyzed fordifferential expression, approximately 69% were differentiallyexpressed.

[0421] Each EST isolated from each of the above libraries represents asequence from a partial mRNA transcript, since the cDNA used for makingthe subtracted library was restricted with RsaI, a four base cutterrestriction endonuclease that generates fragments with an average sizeof about 600 base pairs.

Validation of Differential Expression in Colon Cancer

[0422] To validate that the differentially expressed sequences found inthis library were specific to colon cancer, the clones were screenedwith cDNAs prepared from a colon cancer specific library, Delaware (DE),and a normal tissue specific library Maryland (MD).

[0423] cDNA clones were analyzed for differential expression followingthe procedure developed by von Stein et al., 1997, Nucleic AcidsResearch 25(13):2598-2602 and using probes synthesized according to apublished method (Jin et al., 1997, Biotechniques 23:1083-1086). Out ofthe 1248 clones that were analyzed for differential expressionapproximately 83% were differentially expressed.

Sequencing and Analysis of Differentially Expressed Clones

[0424] The nucleotide sequence of the inserts from clones shown to bedifferentially expressed was determined by single-pass sequencing fromeither the T7 or M13 promoter sites using fluorescently labeleddideoxynucleotides via the Sanger sequencing method. Sequences wereanalyzed according to methods described in the text (XI., Examples; B.Results of Public Database Search).

[0425] Each nucleic acid represents sequence from at least a partialmRNA transcript. The nucleic acids of the invention were assigned asequence identification number (see attachments). The nucleic acidsequences are provided in the attached Sequence Listing.

[0426] An example of an experiment to identify differentially expressedclones is shown in the Figure, “Differential Expression Analysis”. Theinserts from subtracted clones were amplified, electrophoresed, andblotted on to membranes as described above. The gel was hybridized withRSA1 cut DE and MD cDNA probes as described above.

[0427] In the Figure, individual clones are designated by a number atthe top of each lane; the blots are aligned so that the same clone isrepresented in the same vertical lane in both the upper (“Cancer Probe”)and lower (“Normal Probe”) blot. Lanes labeled “O” indicate clones thatare overexpressed, i.e., show a darker, more prominent band in the upperblot (“Cancer Probe”) relative to that observed, in the same lane, inthe lower blot (“Normal Probe”). The Lane labeled “U” indicates a clonethat is underexpressed, i.e., shows a darker, more prominent band in thelower blot (“Normal Probe”) relative to that observed, in the same lane,in the upper blot (“Cancer Probe”). The lane labeled “M”, indicates aclone that is marginally overexpressed in cancer and normal cells.

[0428] B. Results of Public Databases Searches

[0429] The nucleotide sequence of SEQ ID Nos. 1-544 were aligned withindividual sequences that were publicly available. Genbank and divisionsof GenBank, such as dbEST, CGAP, and Unigene were the primary databasesused to perform the sequence similarity searches. The patent database,GENESEQ, was also utilized.

[0430] A total of 544 sequences were analyzed. The sequences were firstmasked to identify vector-derived sequences, which were subsequentlyremoved. The remaining sequence information was used to create theSequence Listing (SEQ ID Nos. 1-544). Each of these sequences was usedas the query sequence to perform a Blast 2 search against the databaseslisted above. The Blast 2 search differs from the traditional Blastsearch in that it allows for the introduction of gaps in order toproduce an optimal alignment of two sequences.

[0431] A proprietary algorithm was developed to utilize the output fromthe Blast 2 searches and categorize the sequences based upon highsimilarity (e value<1e-40) or identity to entries contained in theGenBank and dbEST databases. Three categories were created asfollows: 1) matches to known human genes, 2) matches to human ESTsequences, and 3) no significant match to either 1 or 2, and therefore apotentially novel human sequence.

[0432] Those skilled in the art will recognize, or be able to ascertain,using not more than routine experimentation, many equivalents to thespecific embodiments of the invention described herein. Such specificembodiments and equivalents are intended to be encompassed by thefollowing claims.

[0433] All patents, published patent applications, and publicationscited herein are incorporated by reference as if set forth fully herein.TABLE 1 SEQ ID NO clone name Tissue Probe  1 de0020t7 U  2 de0041t7 N  3de0056t7 U  4 de0064t7 N  5 de0092t7 U  6 de0142t7 N  7 de0153t7 M  8de0163t7 U  9 de0188t7 N  10 de0190t7 U  11 de0201t7 M  12 de0225t7 U 13 de0246t7 U  14 de0257t7 N  15 de0285t7 O  16 de0529t7 U  17 de0629t7U  18 de0727t7 O  19 de0787t7 U  20 de0810t7 N  21 de0833t7 N  22pa0107t7 U  23 pa0130t7 U  24 pa0149t7 U  25 pa0185t7 U  26 pa0203t7 U 27 pa0277t7 U  28 pa0287t7 U  29 pa0293t7* U  30 pa0341t7 U  31pa0357t7 N  32 pa0361t7 U  33 pa0404t7 U  34 pa0408t7 U  35 pa0425t7 N 36 de0001t7 N  37 de0002t7 N  38 de0036t7 N  39 de0038t7 M  40 de0040t7N  41 de0043t7 O  42 de0044t7 N  43 de0045t7 N  44 de0050t7 N  45de0052t7 N  46 de0054t7 N  47 de0055t7 N  48 de0059t7 O  49 de0060t7 N 50 de0063t7 U  51 de0066t7 O  52 de0067t7 O  53 de0079t7 N  54 de0085t7N  55 de0089t7 N  56 de0095t7 N  57 de0099t7 N  58 de0105t7 N  59de0112t7 N  60 de0114t7 N  61 de0121t7 N  62 de0122t7 N  63 de0124t7 N 64 de0139t7 M  65 de0143t7 N  66 de0166t7 U  67 de0168t7 N  68 de0171t7N  69 de0178t7 N  70 de0180t7 O  71 de0181t7 N  72 de0199t7 N  73de0200t7 N  74 de0202t7 N  75 de0205t7 N  76 de0207t7 U  77 de0212t7 N 78 de0217t7 N  79 de0220t7 U  80 de0228t7 N  81 de0236t7 O  82 de0243t7N  83 de0253t7 O  84 de0258t7 N  85 de0259t7 N  86 de0262t7 N  87de0270t7 N  88 de0275t7 N  89 de0287t7 N  90 de0288t7 N  91 de0306t7 N 92 de0490t7 N  93 de0501t7 M  94 de0516t7 N  95 de0589t7 N  96 de0596t7U  97 de0600t7 N  98 de0609t7 U  99 de0611t7 N 100 de0617t7 U 101de0633t7 N 102 de0643t7 N 103 de0647t7 M 104 de0652t7 N 105 de0666t7 N106 de0695t7 U 107 de0705t7 N 108 de0706t7 M 109 de0708t7 N 110 de0724t7N 111 de0735t7 N 112 de0740t7 N 113 de0742t7 N 114 de0747t7 N 115de0764t7 N 116 de0777t7 O 117 de0781t7 N 118 de0793t7 U 119 de0794t7 N120 de0798t7 N 121 de0800t7 O 122 de0816t7 N 123 de0818t7 N 124 de0835t7N 125 pa0078t7 U 126 pa0080t7 N 127 pa0088t7 U 128 pa0089t7 U 129pa0095t7 U 130 pa0158t7 U 131 pa0159t7 U 132 pa0187t7 N 133 pa0190t7 U134 pa0192t7 U 135 pa0209t7 U 136 pa0215t7 N 137 pa0218t7 N 138 pa0220t7N 139 pa0238t7 N 140 pa0249t7 U 141 pa0256t7 N 142 pa0258t7 U 143pa0272t7 N 144 pa0283t7 N 145 pa0295t7 N 146 pa0309t7 U 147 pa0314t7 N148 pa0317t7 N 149 pa0319t7 N 150 pa0323t7 N 151 pa0333t7 N 152 pa0336t7N 153 pa0353t7 N 154 pa0363t7 N 155 pa0364t7 N 156 pa0366t7 U 157pa0382t7 N 158 pa0383t7 N 159 pa0388t7 N 160 pa0389t7 N 161 pa0405t7 N162 pa0406t7 N 163 pa0409t7 U 164 pa0411t7 N 165 pa0417t7 N 166 pa0421t7U 167 pa0429t7 U 168 pa0432t7 U 169 de0004t7 U 170 de0008t7 ND 171de0009t7 ND 172 de0010t7 ND 173 de0011t7 ND 174 de0012t7 ND 175 de0013t7ND 176 de0014t7 ND 177 de0016t7 ND 178 de0017t7 ND 179 de0018t7 M 180de0019t7 ND 181 de0023t7 O 182 de0024t7 N 183 de0029t7 ND 184 de0030t7ND 185 de0032t7 ND 186 de0033t7 O 187 de0034t7 ND 188 de0035t7 ND 189de0042t7 ND 190 de0047t7 ND 191 de0048t7 N 192 de0049t7 ND 193 de0051t7O 194 de0053t7 ND 195 de0065t7 ND 196 de0068t7 N 197 de0069t7 ND 198de0071t7 N 199 de0072t7 ND 200 de0076t7 U 201 de0077t7 ND 202 de0078t7ND 203 de0080t7 ND 204 de0082t7 ND 205 de0086t7 ND 206 de0087t7 ND 207de0088t7 ND 208 de0093t7 N 209 de0094t7 ND 210 de0097t7 O 211 de0098t7ND 212 de0100t7 ND 213 de0101t7 ND 214 de0102t7 ND 215 de0106t7 ND 216de0109t7 U 217 de0110t7 N 218 de0111t7 N 219 de0113t7 ND 220 de0115t7 O221 de0117t7 ND 222 de0118t7 U 223 de0119t7 ND 224 de0123t7 ND 225de0125t7 ND 226 de0126t7 ND 227 de0129t7 ND 228 de0130t7 U 229 de0131t7O 230 de0132t7 ND 231 de0134t7 O 232 de0135t7 ND 233 de0137t7 M 234de0138t7 ND 235 de0140t7 ND 236 de0141t7 ND 237 de0145t7 ND 238 de0146t7O 239 de0148t7 ND 240 de0149t7 ND 241 de0151t7 O 242 de0152t7 ND 243de0154t7 ND 244 de0156t7 ND 245 de0157t7 U 246 de0158t7 ND 247 de0159t7N 248 de0162t7 ND 249 de0169t7 U 250 de0170t7 O 251 de0174t7 ND 252de0176t7 ND 253 de0177t7 O 254 de0182t7 ND 255 de0183t7 ND 256 de0184t7ND 257 de0186t7 ND 258 de0187t7 M 259 de0189t7 ND 260 de0191t7 M 261de0192t7 ND 262 de0193t7 ND 263 de0195t7 N 264 de0196t7 N 265 de0197t7 N266 de0198t7 ND 267 de0203t7 ND 268 de0208t7 ND 269 de0209t7 N 270de0210t7 N 271 de0211t7 ND 272 de0213t7 ND 273 de0214t7 ND 274 de0215t7ND 275 de0218t7 ND 276 de0221t7 ND 277 de0223t7 O 278 de0227t7 ND 279de0229t7 O 280 de0230t7 ND 281 de0232t7 ND 282 de0234t7 ND 283 de0235t7ND 284 de0237t7 ND 285 de0238t7 ND 286 de0239t7 N 287 de0241t7 N 288de0242t7 O 289 de0244t7 N 290 de0247t7 O 291 de0252t7 ND 292 de0255t7 N293 de0256t7 ND 294 de0260t7 N 295 de0261t7 N 296 de0263t7 N 297de0264t7 ND 298 de0265t7 ND 299 de0266t7 O 300 de0267t7 N 301 de0268t7ND 302 de0272t7 ND 303 de0273t7 ND 304 de0274t7 N 305 de0276t7 O 306de0277t7 M 307 de0279t7 N 308 de0280t7 ND 309 de0281t7 N 310 de0282t7 ND311 de0284t7 ND 312 de0286t7 ND 313 de0339t7 ND 314 de0483t7 ND 315de0484t7 M 316 de0491t7 ND 317 de0499t7 ND 318 de0507t7 M 319 de0511t7 O320 de0519t7 ND 321 de0520t7 N 322 de0522t7 ND 323 de0524t7 M 324de0530t7 ND 325 de0531t7 ND 326 de0532t7 M 327 de0534t7 N 328 de0542t7ND 329 de0556t7 M 330 de0557t7 ND 331 de0559t7 U 332 de0562t7 ND 333de0566t7 U 334 de0567t7 N 335 de0568t7 ND 336 de0570t7 ND 337 de0571t7ND 338 de0574t7 ND 339 de0581t7 ND 340 de0583t7 U 341 de0587t7 ND 342de0588t7 ND 343 de0591t7 ND 344 de0592t7 ND 345 de0597t7 U 346 de0598t7ND 347 de0599t7 ND 348 de0602t7 N 349 de0605t7 ND 350 de0608t7 ND 351de0610t7 ND 352 de0616t7 O 353 de0619t7 U 354 de0620t7 ND 355 de0622t7ND 356 de0623t7 ND 357 de0624t7 O 358 de0625t7 ND 359 de0628t7 ND 360de0630t7 ND 361 de0631t7 ND 362 de0632t7 N 363 de0634t7 ND 364 de0639t7ND 365 de0642t7 ND 366 de0649t7 ND 367 de0650t7 N 368 de0656t7 N 369de0657t7 ND 370 de0660t7 ND 371 de0661t7 O 372 de0662t7 O 373 de0664t7ND 374 de0665t7 ND 375 de0667t7 ND 376 de0669t7 ND 377 de0676t7 ND 378de0686t7 N 379 de0687t7 ND 380 de0689t7 N 381 de0691t7 M 382 de0693t7 ND383 de0703t7 ND 384 de0704t7 M 385 de0707t7 O 386 de0709t7 O 387de0710t7 ND 388 de0712t7 N 389 de0715t7 ND 390 de0719t7 N 391 de0722t7ND 392 de0723t7 ND 393 de0725t7 N 394 de0728t7 ND 395 de0729t7 ND 396de0731t7 ND 397 de0732t7 ND 398 de0737t7 ND 399 de0739t7 M 400 de0741t7ND 401 de0744t7 N 402 de0746t7 ND 403 de0749t7 N 404 de0750t7 ND 405de0756t7 ND 406 de0759t7 ND 407 de0761t7 O 408 de0762t7 ND 409 de0766t7ND 410 de0768t7 U 411 de0769t7 ND 412 de0772t7 ND 413 de0776t7 ND 414de0779t7 ND 415 de0785t7 ND 416 de0786t7 ND 417 de0788t7 ND 418 de0789t7ND 419 de0792t7 ND 420 de0796t7 ND 421 de0797t7 ND 422 de0801t7 O 423de0804t7 ND 424 de0805t7 ND 425 de0806t7 ND 426 de0807t7 N 427 de0811t7O 428 de0812t7 ND 429 de0817t7 N 430 de0820t7 ND 431 de0821t7 ND 432de0822t7 ND 433 de0823t7 N 434 de0824t7 N 435 de0825t7 ND 436 de0826t7ND 437 de0827t7 ND 438 de0829t7 ND 439 de0830t7 ND 440 de0837t7 N 441de0840t7 ND 442 de0848t7 ND 443 pa0079t7 N 444 pa0081t7 ND 445 pa0082t7ND 446 pa0083t7 ND 447 pa0084t7 ND 448 pa0085t7 ND 449 pa0086t7 M 450pa0090t7 N 451 pa0091t7 ND 452 pa0092t7 N 453 pa0096t7 ND 454 pa0100t7ND 455 pa010It7 U 456 pa0103t7 ND 457 pa0104t7 ND 458 pa0114t7 ND 459pa0115t7 ND 460 pa0I18t7 ND 461 pa0120t7 ND 462 pa0129t7 ND 463 pa0131t7U 464 pa0133t7 ND 465 pa0135t7 N 466 pa0140t7 O 467 pa0142t7 ND 468pa0143t7 ND 469 pa0146t7 ND 470 pa0147t7 ND 471 pa0148t7 ND 472 pa0151t7ND 473 pa0157t7 ND 474 pa0164t7 ND 475 pa0167t7 N 476 pa0171t7 U 477pa0174t7 ND 478 pa0175t7 ND 479 pa0179t7 N 480 pa0182t7 ND 481 pa0184t7ND 482 pa0186t7 U 483 pa0189t7 ND 484 pa0207t7 ND 485 pa0210t7 ND 486pa0212t7 ND 487 pa0214t7 ND 488 pa0216t7 ND 489 pa0217t7 M 490 pa0219t7N 491 pa0223t7 ND 492 pa0224t7 ND 493 pa0228t7 ND 494 pa0229t7 U 495pa0231t7 ND 496 pa0232t7 ND 497 pa0240t7 ND 498 pa0252t7 ND 499 pa0260t7U 500 pa0261t7 N 501 pa0262t7 ND 502 pa0264t7 N 503 pa0265t7 N 504pa0268t7 ND 505 pa0276t7 ND 506 pa0279t7 ND 507 pa0280t7 ND 508 pa0282t7ND 509 pa0285t7 ND 510 pa0299t7 ND 511 pa0300t7 U 512 pa0301t7 ND 513pa0302t7 ND 514 pa0305t7 N 515 pa0306t7 ND 516 pa0307t7 ND 517 pa031It7ND 518 pa0316t7 ND 519 pa0318t7 ND 520 pa0321t7 M 521 pa0325t7 N 522pa0326t7 ND 523 pa0332t7 ND 524 pa0339t7 ND 525 pa0346t7 O 526 pa0349t7ND 527 pa0351t7 U 528 pa0355t7 ND 529 pa0358t7 ND 530 pa0360t7 N 531pa0362t7 ND 532 pa0368t7 U 533 pa0369t7 ND 534 pa0373t7 ND 535 pa0380t7ND 536 pa0393t7 ND 537 pa0395t7 ND 538 pa0396t7 ND 539 pa0397t7 ND 540pa0410t7 N 541 pa0415t7 ND 542 pa0416t7 ND 543 pa0424t7 ND 544 pa0430t7ND

[0434] TABLE 2 “Novel” Region 1 “Novel” Region 2 SEQ ID NO Clone nameStart/Stop Start/Stop GenBank Identifier for top 5 matching ESTsequences 36.00 de0001t7 439-607 g835668 g857149 g1321047 g1968601g1476832 40.00 de0040t7  1-201 g2166831 g4136486 91747976 g1180529g2265195 41.00 de0043t7 467-615 g5129477 g1801229 g1845053 g154468391694347 43.00 de0045t7  1-228 g2322205 g1139955 g4267203 g2165927g3039227 45.00 de0052t7 455-628 g1523492 g1548890 g1523465 g1809433g5132985 50.00 de0063t7  1-114 452-624 g2197338 g5754794 g269444892070840 g3419233 51.00 de0066t7 301-631 g2162184 g749398 g1239250g839454 91966148 52.00 de0067t7 391-623 g1521548 g848102 g134941991196287 g771178 54.00 de0085t7 415-565 91367045 g1367136 g2337716g841637 g795336 63.00 de0124t7 411-605 g1809451 g1757444 g3181138g2905518 g1157799 64.00 de0139t7 424-612 g3899105 g3431615 g3246439g1312989 g1182375 65.00 de0143t7 479-598 g1239204 g1067288 g1080541g4876470 g1188553 68.00 de0171t7 443-611 g867521 g1636718 g2162333g2342197 g1466482 69.00 de0178t7 485-603 g1371240 g2055704 g2208007g1686872 g1740908 71.00 de0181t7  1-153 g1188057 g1018287 g1447796g1025264 g1069169 73.00 de0200t7  1-218 384-581 g1972267 g1989383g964966 g2883986 g483738 74.00 de0202t7 448-599 g2115372 g1959491g1329334 g1198642 g1957432 75.00 de0205t7 1 to 75 g779809 g2167738g2537620 g2656428 77.00 de0212t7  1-185 g4265939 g1548503 g1687914g1716864 g877386 80.00 de0228t7 411-594 g3446139 g3745043 g1126367g2163321 g1195781 82.00 de0243t7 253-604 g2001999 g1071313 g966668g26974 83.00 de0253t7  1-133 g2111781 g1663818 g574791 g1406232 g166381285.00 de0259t7 241-602 g2216159 g5177204 g1969363 g1388290 g138946486.00 de0262t7 351-583 g1025700 g2019225 g2080424 g1547366 g728148 88.00de0275t7 455-592 g5665082 g5553136 g5552975 g389141 g1665092 89.00de0287t7 364-630 g2026446 g4622337 g2021046 g2056125 g5037418 92.00de0490t7  1-264 482-653 g1812285 g2816130 g2818085 g2819140 g119426096.00 de0596t7 362-655 g1155862 g1991972 g1996949 g1149020 g330733197.00 de0600t7 1 to 71 g883470 g1880085 g3162627 g3162628 g918039 98.00de0609t7 434-582 g5037002 g1404408 g2816378 g759987 g2969638 102.00de0643t7 433-605 g1382697 g5236495 g5235876 g5177792 g4453929 105.00de0666t7 385-586 g2932996 g1010052 g2616680 g3277252 g2252166 106.00de0695t7 401-644 g4897106 g1741190 g1501550 g3932286 g781364 107.00de0705t7 431-599 g5176918 g1551249 g4739742 g2540062 g2583382 108.00de0706t7 407-620 g826820 g870098 g1685556 g1687983 g685342 110.00de0724t7 127-192 423-603 g2036407 g1969842 g616903 g1218717 g1745366113.00 de0742t7 1 to 49 g2596046 g3277962 g4665052 g895453 g1521473116.00 de0777t7 270-610 g2358992 g1692100 g1979572 g1720777 g1547963117.00 de0781t7 457-608 g1982258 g1379170 g1496197 g1981084 g1982434118.00 de0793t7 435-577 g1860678 g1991856 g946392 g4703537 g5741112119.00 de0794t7 342-584 g4394682 g3418148 g3424422 g1398292 g4985474120.00 de0798t7 1 to 65 384-585 g1570282 g4817443 g3891031 g2028723g2357411 121.00 de0800t7 270-568 g2358992 g1692100 g1979572 g1720777g1692006 122.00 de0816t7 485-550 g2014274 g2189652 g1087845 g2013302g643722 123.00 de0818t7 387-573 g4190027 g4900502 g3601481 g4070577g4112474 124.00 de0835t7 330-570 g2029304 g2029457 g1544689 g1947895g2986865 129.00 pa0095t7  1-370 g5340876 g771049 g791906 g677786 132.00pa0187t7 388-593 g2029457 g2029304 g1544689 g1947895 g2986865 134.00pa0192t7 444-618 g4897608 g1815096 g2051120 g3426889 g4690585 139.00pa0238t7 364-586 g4728995 g4971678 g1615267 g1501282 g5447095 140.00pa0249t7 124-588 g2061363 g2060961 g2060863 g1135265 g2060372 142.00pa0258t7 254-595 g2784639 g2276958 g968701 g1670101 g2329615 143.00pa0272t7 1 to 98 g2198976 g1961065 g2188645 g1965162 g1852287 148.00pa0317t7 457-612 g5113829 g2080750 g3739118 g3753615 g2933157 153.00pa0353t7 357-620 g2029457 g2029304 g2986865 g1544689 g1947895 156.00pa0366t7 354-760 g5339118 g775873 g610250 g3886660 g2052048 157.00pa0382t7 395-668 g3886319 g968094 g2216376 g4126052 g1747689 159.00pa0388t7 492-739 g1636721 g2006249 g5035641 g3430502 g778670 160.00pa0389t7  1-177 g1321159 g1320039 g1371307 g2070781 g1350314 161.00pa0405t7 119-214 g4824527 g4852801 g1368093 g1392201 g1350038 164.00pa0411t7 289-345 g4810371 g2369264 g3163382 g3839554 g1950020 166.00pa0421t7 233-745 g5747013 g4150749 g1482715 g1137706 g3900569

[0435] TABLE 3 The following list of clones indicates those found ineither the DE or PA libraries and the SW480 library SEQ ID NO clone name185 de0032t7 186 de0033t7 193 de0051t7 196 de0068t7 240 de0149t7 241de0151t7 247 de0159t7  72 de0199t7 279 de0229t7 281 de0232t7 283de0235t7 306 de0277t7 310 de0282t7 318 de0507t7 328 de0542t7 331de0559t7 342 de0588t7 359 de0628t7 375 de0667t7 379 de0687t7 407de0761t7 410 de0768t7 427 de0811t7 466 pa0140t7 470 pa0147t7 481pa0184t7 493 pa0228t7 494 pa0229t7 140 pa0249t7 506 pa0279t7 510pa0299t7 515 pa0306t7 517 pa0311t7 518 pa0316t7 536 pa0393t7 539pa0397t7 544 pa0430t7

[0436]

1 544 1 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C or G 1gcgtggtcng gccgaggtac agtctcggct cactgcaatc tctgcctgcc cagntcaagc 60gattctccat catgttggcc aggctggtct caaattcctg aggtgatctg cccacctctg 120cctgccaaag tgctgggatt acaggtgttg agcgatagtg ctcggcctat tatttctttt 180taaatctttg gtagaattaa tcactgaaac tatntgtgct ttttttgnng gaaaaattat 240ttattttaaa gacagggtct tgntctgttg cctgtgctgg antgcagtgg tgcaatctca 300gtttactgca accttgtgcc aacctactgn caagtgatcc tactgnctca cctccnagta 360ncttggatta caggcacgcg ccaccatgcc cngntaggtn ttgnattttt aggagaaacn 420gggtttcatn atattggnca gcnnggcttg agcttctgaa ctcaantgat ccnccncctc 480ggcctnccaa acactgggat tacaggcgtg agccctcccc tgntgatacg nagnggtttt 540aanaagattn tcttcaantt ngtttaaaaa ttctaatttn ngaccatttt tnctgcccgc 600ggcgnnaaag gcnaatcn 618 2 640 DNA Homo sapiens misc_feature (1)...(640)n = A,T,C or G 2 actttttttt tttttttttt tttttgagac agggtcttgc tttgtcacctgggctgaagt 60 gcagtggcat gatcatggct cactgcagcc tcaacctcct aggctcaagtgattctctca 120 cctcctcctc ttgagtagat gggacttaca ggcgcatgcc accacatgcagataattttt 180 gtattttttg tanaaacagg gttttgccat gttacccaaa ctggtcccaagctcctgggc 240 tcaagagatc tgcctgcccc aacctcccaa agtgctggga attacaggcattgagccacc 300 acacccagcc tgattgtttc ttctcataac tcaactctac tgntgatcctctttaatgaa 360 ttttantttc aagtcattct acttttccac tccaaaattt tgatttgggtcttttaaata 420 aattttattt attggaattc tttatttggg gagaaggtat catatattcctttanttctt 480 ttggcgngct ttcttttaac tctttgatat ttataatagc tgntttgaaagctttttntg 540 gtaagtccaa cattngggnc ctcaangctt ttttaatgct gctttttttcccctattatg 600 gnaaacttcc agttatttta tgctaataag gttcggaaaa 640 3 635 DNAHomo sapiens misc_feature (1)...(635) n = A,T,C or G 3 acactagcataaatgaagat taagaaataa gtctttccag attattttta ctcaagaatt 60 tgtttcagtgctaggcaagg atgatcaatt ttagtttgca tatgaagact caaagggaga 120 tgattaaaagcacgtaactc tttgactcac ccctagaagg tctttgatga ggcccagcaa 180 tctggaaaattatgatataa tattacacaa tgattattta acaatatttt agaagtaact 240 gccatttgggggtcacagaa caatactaat ctcaattatg ttacccatca acaaattgaa 300 tataattaaattattttcaa aatatatggg ttgagattat tttccaatta aaattgccag 360 gtgaggaacagcacttttcc attcgctgct gaatgtgatg aaatactgga tagtcataga 420 gggtctacccagatgtccct ttgggagaag tgttgtgggg gaaaatgggc tggttgtgtg 480 cacccaaactaccctttaag aacttggtgc tggagccatt aaaaataatt gngctggtct 540 tataaatatgaaaaactttg ggaaatcctt gtgacatcga tgcanttggg ttgggaagtt 600 cctgataaaaatatctaaaa atacacccat tgaaa 635 4 627 DNA Homo sapiens misc_feature(1)...(627) n = A,T,C or G 4 acgcggggac taagacctca aaagcacagg cgacaaaaataaaaatagac aaatgggaat 60 taactaagaa gtttctacac aacaaaagaa ataatcaacagagtaaacag ataatttcca 120 gaacggaaga aaatatgtgc aaactattca tccagcagtggacaaatacc cagtatatac 180 aagaaactca aacaacaaca ataaaagaca aatcatccccttaaaaggag ggcaaaagac 240 aagaacagac atttttcaaa agaagatata caaatgactaacaggtatat taaaaatgca 300 caacatcact aatcatcaga gaaatgcaaa ttaaaaccacaatgagatat catcttaccc 360 cagtcaaaat ggctactatt aaagagtcaa aaaataatagatcttggcca ggacatggat 420 aaaagagaac tcttacatac tggtggtagg aatgcaaattaacacagcct ctatagaaaa 480 cagtatngag attgctcaag aactaaaaat agagctatcatttgcccanc atnccctgnt 540 gggttctacc caangaaaag aaatcatggt caaaaaaaaaaaaaaaaaaa aaagtncttg 600 gcgggaccct aaggggattc acccctn 627 5 411 DNAHomo sapiens misc_feature (1)...(411) n = A,T,C or G 5 cgaggtacgcggcctggcca acatggtgaa accccgtctc taccaaagat acaaaaaatt 60 agccaggtgtggtggcatgt gcctgtagtc ccagctactc aggaggatga ggcaggggaa 120 tcacttaaacctgggaggcg gagattgcag tgagccaaga tcgcgctatt gcactctagc 180 ctgggtgacagagcaagact ccgtctcaaa aaataataat aataaaatga aaataatcag 240 ctgggtgtggtggcatgtgc ctgtagtccc agctactcag gaggatgagg caggggaatc 300 acttaaacctgggaggcgga gattgcagtg agccaagatc gcgctattgc actccagcct 360 gatgacagacctagactccg tctccaaaaa aaaaaaanaa aaaaaaaaag t 411 6 606 DNA Homo sapiensmisc_feature (1)...(606) n = A,T,C or G 6 accaagtcac cagacacaagtaaatatggg ccttggggct tcttttcttg ctgacgcata 60 ttcacacagg cagcgtgtgctgtgtgtgtt tacaactgtg tttcttagtc ttctattcag 120 agtaataaca gcatgacttccctaagatct gattcagaga attgaaatat gccctgagaa 180 aacataagag gtttttctggagaagtgtcc caagggtaat attaattgtt caaggatgtt 240 tcggaaaaag ttgcaatcatcactgtggca aatgaatcta gggagaggaa gcatgagtta 300 tttaatgtca gttactcctttccgtaggtt tttgcctttt tttggacttt acacacagcc 360 catttgctat gaaactatcagctcaaatag cangctttca ngcaggccaa caatggcaga 420 ctgcattctt nctactttntccaatcatat ttatcaagtc ccattgggag aatactttca 480 gtagngctca aantacccgcntncaattgg aactgcangg aaccnttcag aaataacnct 540 tnaagaaaga aataacccttcanggaanac cctttnggnt tcactctann tggggttnac 600 aagaaa 606 7 620 DNAHomo sapiens misc_feature (1)...(620) n = A,T,C or G 7 ccgtggccccggccgaaggt ccnctgganc cccgggtggt aattggctgg aggtaaatgg 60 tggtaangtattaaccattt ctatggaaat gnccctttgg ggccctcctg gattttaaaa 120 tggtcccctggtttggacnt ttctattaaa gaaatggnca ttttacctaa aatgccnggt 180 ctaccttattaaagancaaa tngnntattn gaccttaaaa taggcatttt tcctaatcat 240 aatctggccggcttaacccc aatcaagata attgggtgcc cnttatgaat ttgaagttag 300 tgatagcctccttgtaaggt gctaccctna tggggataga gaccccagct actantaatt 360 ngggaaaatggttaaggtat ttgggaaaag tactctttta aaaacatatt ggccacagaa 420 ancctaggctgaattacnng gattgataat tttgnaanta atttcntana atgggcnngc 480 tggatgaaaaaatggcctcc tcnttttccc tggaaccagc ngctttttgc ctaaacntta 540 ncctttttaagttgaaccta gggaccacct aatnggcntc acaattccct ttttcctttc 600 ctttttttttgcccaagggn 620 8 263 DNA Homo sapiens 8 gcgtgggtcg cggccgaggt accacttttcttattgcaac tcaacaagtg gcaattggtg 60 atgaaaagtc aagtggggaa cccagtctgtggggaacaaa tggaataact tacctgtcac 120 cttgtctaac cgggatgcaa atcctcaagtggtattaaaa agcatacagt gttttataac 180 tgtagttgtg tggaaagtaa ctggtctccaagaacagaaa ttactcagcg cacttgggtg 240 aatgcccaag aaataatact tgt 263 9 590DNA Homo sapiens misc_feature (1)...(590) n = A,T,C or G 9 acaacagggttcttgcatca agcttcatgc tttcccagac atttactcaa gggaacgtgg 60 gagagggaggaggaggaggg gagctgggag tgataagcag atgttacaca tgtttttcct 120 ggaaagatcaccccactttt tctaatttcc cagaattaaa agaatgtatt ttatctgtat 180 taccatggaaattactagta acactggatt tttttccctc ttttctaaag tttccaaaaa 240 ctttcaaaagtgttcaaaga aattttcttg aacaatttta atatgtttga tttctcattt 300 ggggctggaatatttgtatt ctttttaatt tttttacttc atttattaga agaagtttct 360 aatatgtgtaggaatacaat tttaaatgta agattatata gatgtagata tagatagata 420 gatatatgtagatatatnga tttatgtcnc aatatcactn taaggcattc ttcttccatc 480 cttttatatctncccaaact ggtntnatgg gacctgtcct gcctgtaggt aaaanccttn 540 taatttccctgaaaggctac cnctttctan ggggncaacc aattgggagn 590 10 609 DNA Homo sapiensmisc_feature (1)...(609) n = A,T,C or G 10 cgaggtacgt ttttcaatgttttaaaaaat tgaaagggag tataatgttt cataacacat 60 gggaaattat gtgcagctcaaatttcaagt atccataaat aaagttttat tggaacacag 120 ctacgctcac tcattagatattgtctatgg ctgtttttgt gcaaaatggc aganttgggt 180 tcagagttag caacagagagcttgtagcct gcaagcctag agtatttact atctggattt 240 ctacagaaaa aaaaaattattgccccctgc catacagtct gactgatagc ctgagaaagt 300 atgcattaaa agaaagttacctaccctgac cccatgagaa tgaatttgaa aagaaccnag 360 atgtggtaga agcagataggctatgaaagt ttcagaaggg tancatcact gtgggcnagg 420 atattcaaga aaagacttcanggaaaatgt nggggtttga actggncttg agtaggagtt 480 naacttangg gaactggntttaggtngcca ctttaaggct gtcaaanatc atggcccaac 540 attcantttg gcccaaattccccangngcc ttaaaaattt ggacatggct tgggttgggg 600 gncaccctt 609 11 578 DNAHomo sapiens misc_feature (1)...(578) n = A,T,C or G 11 acgcgggatgtagtcagagg aggccctgac atctgcaggg cagcatgggt caaaccaaaa 60 agacttttctgaggttgggc gcagtggctc acgcctgtaa tcccaacact ttggaaggcc 120 agtaggggcggatcacctga ggtcaggaga ttcgagacca tcctggctaa cacggtgaaa 180 ccccatctctactaaaaaaa atacgaaaaa aattagccag gcgtggtgac gggtgcctgt 240 agtcccagctactagggagg ctgaggcagg agaatggtgt gaacccggga ggcagagctt 300 gcagtgagccgagatcaggc cactgcactc cagcctgggc cacaagagcg agactctgtc 360 ttaaaaaaaacaaacaaaca aacacacaca cacacacaan aagacaaaaa taattagcag 420 ggaatgctggtgcatgcctg tatcccaact ctcaggaggt tgaagcagga gaatcacctt 480 gacccatnagcaatgttcat gaacttagnc cngccntgga cttcancaag gcaccgagta 540 aganttcntttnaaaaaaaa aannnaaaaa aaagtcct 578 12 581 DNA Homo sapiens misc_feature(1)...(581) n = A,T,C or G 12 actttttttt tttttttttt ttttttttttgggacggagt cttgctctgt tgcccaggct 60 ggagtgcagt ggcgccatct tggctcactacaagctccgc ctcccgggtt cacaccattc 120 tcctgtctta gcctcccagc gcccgccaccgcacccggct aattttttgt atttttagta 180 gagacagggt ttcaccatgt tagccaggatggtctcgatc tcctgacctc gtggcccacc 240 tgccttggcc tccaaaagtg ctggaattacagtcgtgagc caccacgccc ggcctaaacc 300 atttctcttg acaacactct ggattttatttctggccaga taccatttat caattttacc 360 atcaagaata agataatcaa aataataatcaagttttata ttagacttat gaagattctt 420 gcacctttga aattacagct atctcactagttnattctcc tctctcatat tttattacng 480 acntccagga agacaaccaa cacctttaaaagttggctga gcatttttta nggagaccct 540 taggtaanag ggncctnggc gggaaccccttaggggnaat n 581 13 607 DNA Homo sapiens misc_feature (1)...(607) n =A,T,C or G 13 ggtactggaa caactataag acccctgttc agattaagga atttggtgcagtttcaaaag 60 tagacttttc tcctcagcct ccatataatt atgctgtcac agcttcctcaagaattcaca 120 tttatggccg atactcccaa gaacctataa aaaccttttc tcgatttaaagacacagcat 180 actgtgctac ttttcgacaa gatggtagat tgcttgtggc tggcagtgaagatggtggag 240 ttcaactttt tgatataagt gggagggctc ccctcaggca gtttgaaggccatacaaaag 300 cagttcatac agtagatttt acagctgaca aatatcacgt ggtctctggggctgatgatt 360 atacagttaa attatgggat attccaaact ccaaagaaat ttttgacatttaaaggaaca 420 ctctgattat gtgangtgtg gatgtgctag caaactttaa tccggatctctttataacca 480 gggacatatg atcatactgn gaagatgttg gatgcncgaa ccnattgaaaagtggtcttt 540 ccgttgagca tggccnncag tngaaantgn cctacttttc cccttggaaggctttggggt 600 annangg 607 14 599 DNA Homo sapiens misc_feature(1)...(599) n = A,T,C or G 14 ggtactttca aaatataaca attttcgttctcccatataa cagggggcta acaagaaaac 60 caaaaataaa taaaaagaga aaatttaaaaataagtaaaa aataaaaaaa tatttttaaa 120 aagcagcctg ggcaagagaa gtgggtgggtttaggagaat ccctttcgaa aaattcagag 180 cattattatt aatcgttctt aaattaaatgcagggccaag catgctgcac gtggaatctg 240 gacaattttt tgataaactt taaggctgctaaataattta cagaaactgt gaatgcattt 300 tcattttacg aggcaaaaga gaaaatattcaagattgcat agcaatttta ttttttgaaa 360 tggttatcct aaagaatttc cttaaattcagattttgcaa aattcctact ctncaagtca 420 tcaagtgaac actaaaagca actttctcgtgaatcagtgg acttttacga ggcatgcatt 480 tttcataaat ctaggccaag tgacctaattgngattaaat cttaatcatc ctgngattct 540 ggctattaan atgggtttaa ancngtaaaaatnctttnaa aaagccgtta cttnccgan 599 15 457 DNA Homo sapiens misc_feature(1)...(457) n = A,T,C or G 15 ggtacttttt tttttttttt tttttttttcgaaatgaaca aatatttatt tatcttttat 60 aacaagtaag gcaatgttgc ttaaaggaagacaaacaaac ataaaagatt ccgttgacaa 120 tgcatttttt catntgttcg gcacaatgcttttgtcataa tggagatgtg acagcaaact 180 ttccaggaca ttcagtcttc ggnggcagcacttagggcan atgactggcc gctcaaattc 240 tctatnttgt ttcaggacag tggaaaagcttatanatgag gccaaagcac caggtaggtg 300 gaaggttctt gtatcggttc gaaccccgacagcgcgccaa cagacaacac naggcagtgg 360 ggagcaacat gctgttttaa tgancgcctgggtgcangcg tgctgaggct gaaaatggca 420 taacccccgc gtcctgccng gcgggcgttcaaanggn 457 16 643 DNA Homo sapiens misc_feature (1)...(643) n = A,T,Cor G 16 ggtacaatct agctgaaatc atatacaagt aagtaggtgt ggacttttactgctgagcta 60 aagtttatgt ttatatatgt tttattcttt aagctaaaca aacattcagataacattcta 120 tgcatttttt gaagcatagg gttagtaatg aggacttaga ttttttaattaaacaattca 180 gtaactatat aaaaagaaaa ggagtccctt atgaataaat attaaaattaaaagaaatag 240 gcaactataa aagtaagtat ttttaataat ggcattgatt ttagtaagaaatcaattagg 300 ctgggctgga aagaaaaact ggcttaatat aaagtagttt taatatggcaaatattcttc 360 ttaaaattgn ggccctggaa tatcatttct gcctattgct gatgctaaggnatcaactgn 420 gccaagtatt gggctgntcc acaggtggga angagtagca acattttgnggatttttttt 480 tttttttaaa accggagaat acccggccag gggntcaagn ctgnatccacantttgggag 540 nttagccgga naanccttgg anccggagna aaggttnaan gagncaaaatgngccatggn 600 ttccanctgg ggacccgggg gnaactcttt taaaccnaaa aat 643 17336 DNA Homo sapiens misc_feature (1)...(336) n = A,T,C or G 17ggtactttga taaatgtaga aagattattt aattctggct tggtaccgtg gctcatgcct 60ataatcccag cacttcagga ggctgaggtg ggtggatcac ttgagctcag gagtttgaga 120ccaggcgaaa ccctgtctcc acaaaaaatg caaaaattgc tggacatggt ggcacatgcc 180tgtagtccca gctacttgga aggctgaggc aggaggatag cttgagccca ggaggtcaag 240gttgcagtga gccgagattg tgccactgca ctccagcctg ggcaacagag caagaccctg 300cctcaaattt aaaaaaaaaa aannaaaaaa aaaagt 336 18 614 DNA Homo sapiensmisc_feature (1)...(614) n = A,T,C or G 18 ggtacactct tcttcgcctttgagtgccgc tacctggctg ttcagctgtc tcctgccatc 60 cctgtatttg ctgccatgctcttccttttc tccatggcta cactgttgag gaccagtttc 120 agtgaccctg gagtgattcctcgggcgcta ccagatgaag cagctttcat agaaatggag 180 atagaagcta ccaatggtgcggtgccccag ggccagcgac caccgcctcg tatcaagaat 240 ttccagataa acaaccagattgtgaaactg aaatactgtt acacatgcaa gatcttccgg 300 cctcccgggc ctccattgcagcatctgtga caactgtgtg gagcgcttcg accatcactg 360 cccctgggta gggaaatgtgttggaaaaga ggaactaccg ntacttctac ctcttcatcc 420 tttctctttt ccctccttacaaactaaggc tttngctttc aacatcgcta tgtgggccct 480 aaaatctttg aaaattggctttttggaana cattgaaaga aactcctgga aactggtcta 540 gaaagnccta attgcttctttacactttgg nccnncnggg actgatggga tttcanactt 600 tcttgggact ttna 614 19296 DNA Homo sapiens misc_feature (1)...(296) n = A,T,C or G 19actttttttt tttttttttt ttttttttgg gatggagtct cactntgttg ccaaggctgg 60agtgcagtgg cataatttcg gctcacttca acctctgcct cccgggttca agcaattctg 120cgtcagcctc cggaggagct aggactacag gcatgcacca ccatgcccaa ctaatttttg 180natttttagt agagatggag tttcaccata ttgaccaggc taggctggtc ttgaactcct 240agcctnaggt gatctgccca cctnagcccc ccaaagtacc tcggccgtga ccacgc 296 20 565DNA Homo sapiens misc_feature (1)...(565) n = A,T,C or G 20 accaattataatgcattatt atgaaatatt taaaatgggg aatccaagat gacatagttt 60 ttaactcatccacatactgg aagtttagag aaactcagaa tttcttattt ctttttcttt 120 ttcctccatagcataaaagc tttgctaata agaataaata tatatattgg agttttagtg 180 tttgatcctgtgatcagttg taaccatgtg tcataaaact ctctcacaga ttccatcttt 240 cccaaatcttctgatcataa cacagattgc catatagact tcccttgtaa ggagaatatg 300 ctggccataaggcaagcana agtgaacttg cagtttcact tcttggaaat taatgcattt 360 gcattgacttctataannta atctctcctg aatttttttg cttagtcaac ttactgtgtg 420 caaagncaacagnaaattgt ctttggttna acttttaaca ggncaattta taaattggtt 480 tgaagaagcntcccnaaatt ttttattgaa ggctgaattc aagcctccnt taaaatggnc 540 atngnataangggaatttat tgtng 565 21 582 DNA Homo sapiens misc_feature (1)...(582) n= A,T,C or G 21 ggtactggaa caactataag acccctgttc agattaagga atttggcgcagtttcaaaag 60 tagacttttc tcctcagcct ccatataatt atgctgtcac agcttcctcaagaattcaca 120 tttatggccg atactcccaa gaacctataa aaaccttttc tcgatttaaagacacagcat 180 actgtgctac ttttcgacaa gatggtagat tgcttgtggc tggcagtgaagatggtggag 240 ttcaactttt tgatataagt gggagggctc ccctcaggca gtttgaaggccatcaaaagc 300 agttcataca gtagatttta cagctgacaa atatcacgtg gtctctggggctgatgatta 360 tacnagttaa atttatgggg atattncaaa cttccaaaga aaattttgnccatttaaaag 420 aacactctng antatggnga aggtgnggnt tgtgcctaac caaacttaattccgggatct 480 tttttatnta ccnggattcn tttggatctt ncnggtaaaa aanggttggatnccccnaac 540 nnattgaaaa nngttctntc cnnttgacct nggccanccn ng 582 22 349DNA Homo sapiens 22 actttttttt tttttttttt ttttttgaga tggagtcttgctcttgttgc ccaggctgga 60 gcaacctccg cctcctgggt tcaagtgatt ctcctgcctcaacctcccga gtagctggga 120 ttacaggtgc ccgccaccat gccgagctaa tttttgtatccctagtaaag acggagtttt 180 gccatgttgg ccaggctggt ctcgaactcc taacttcatgatctgctcac catggcctcc 240 caaagtgctg ggattacagg cgtgagccac tgtgcccaaccctcttttcc tttttcaaat 300 gtcaatggaa agttgattgg aaaggacaat ttggctaccttttggtacc 349 23 576 DNA Homo sapiens misc_feature (1)...(576) n = A,T,Cor G 23 acctgttctt ggagccaatg tgactgcttt cattgaatca cagaatggacatacagaagt 60 tttggaactt ttggataatg gtgcaggcgc tgattctttc aagaatgatggagtctactc 120 caggtatttt acagcatata cagaaaatgg cagatatagc ttaaaagttcgggctcatgg 180 aggagcaaac actgccaggc taaaattacg gcctccactg aatagagccgcgtacatacc 240 aggctgggta gtgaacgggg aaattgaagc aaacccgcca agacctgaaattgatgagga 300 tactcagacc accttggagg atttcagccg aacagcatcc ggaggtgcatttgtggtatc 360 acaagtccca agccttcctt gcctgaccaa tacccaccaa gtcaaatcacagaccttgat 420 gccacagttc attaggataa gattattctt acatggacag caccaggagataattttgat 480 gttggaaaag ttcaacgtta tatcataaga ataatgccag tattcttgactaagagacag 540 ttttgatgat ctcttaagta aatactctga ntgccn 576 24 618 DNAHomo sapiens misc_feature (1)...(618) n = A,T,C or G 24 acttaaaataaagttaacaa ttacaacaga cccaatcaca gacaatacca gcgtagaaat 60 attaactccagaattatgac ttttatcagg agtaggagta ggagtaggag taggtgtagg 120 atcaatgtcatcaggatttg cttgagggat aaacaaagtt acttgtgcaa tgttggatac 180 ttttgatgtcaaattgcttt tatctatact tttaatggca ataaatatgt gggttgcatt 240 ttcttctgagatattttctg gtttaaatgc aaagctttcc ttggagttgg cctcctttgg 300 tgacagatcagtagtattta cttgaagagc atcatcaaaa ctgtctctta gatcaagaat 360 acttgcacttattcttatga tataacgttg aacttttcca acatcaaaat tatctcctgg 420 tgctgtccatgtaagaataa tcttatcctc atgaactgtg gcatcaaggt ctgtgatttg 480 acttggtgggtattggtcag caagggaagg cttgggactt gtgatccaca aatgccctcc 540 ggatgctgtcggctgaaatc ctccangtgg ctgagtatcc tcatcaattc aggtcttggc 600 nggttgcttcaattnccc 618 25 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,Cor G 25 acataccacg ctgggtagtg aacggggaaa ttgaagcaaa cccgccaagacctgaaattg 60 atgaggatac tcagaccacc ttggaggatt tcagccgaac agcatccggaggtgcatttg 120 tggtntcaca agtcccaagc cttcccttgc ctgaccaata cccaccaagtcaaatcacag 180 accttgatgc cacagntcat gaggataana ttattcttac atggacagcaccaggagata 240 attttgatgt tggaaaagtt caacgntata tcataagaat aagtgcaagtattcttgatc 300 taagagacag ttntgatgat gctcttcaag taaatactac tgatctgtcaccaaaggagg 360 ccaactccaa ngaaagcttt gcntttaaac cagaaaatat ctcagaagaaaatgcaaccc 420 acatatttat tgccnttnaa agtatagata nagcaatttg acatcnaagtntccacattg 480 nacaagtnac tttggttatc cctcagcaaa tctgatgaca ttggatctactctactctac 540 ttctanttct gaaaaaggat aatccggngt aaattttccc tggattgctgggatg 595 26 361 DNA Homo sapiens misc_feature (1)...(361) n = A,T,C orG 26 actttttttt tttttttttt ttttttctga gcatattata tctaattttt gaaggttgta60 ttttctccct tgttttaatt ttctgcanat acttttttct tttttacttt ccccaattag 120tttgtttctg actttcttcc tcaatctctc ctgaaccatt gtttnttttt aagatcagag 180cagattctta ggaactttta aaactgtatg tgggtgggat tgtcacctan agtgcttttt 240tggagagtaa ttggatggng tgataattaa ttttatgtgt caatttgaca gggtcttggg 300gtgtccagtt atttggttaa acattatttc tgggtgtgcc taaaagggtg tcccgcgtac 360 c361 27 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 27acctgttctt ggagccaatg tgactgcttt cattgaatca cagaatggga catacagaag 60ttttggaact tttggataat ggtgcaggcg ctgattcttt caagaatgat ggagtctact 120ccaggtattt tacagcatat acagaaaatg gcagatatag cttaaaagtt cgggctcatg 180gaggagcaaa cactgccagg ctaaaattac ggcctccact gaatagagcc gcgtacatac 240caagctgggt agtgaacggg gaaattgaag caaacccgcc aagacctgaa attgatgagg 300atactcagac caccttggag gatttcagcc gaacagcatc ccgaggtgca tttgtggtat 360cacaaagtcc caaacctttc cttgcctgac caatacccac caagtcaaat cacagacctt 420gatgccacaa gtcattagga taaaatattc ttacatggan gcccangaaa taattttgat 480gttngnaaag ntcaccgtnt ntataanaat aaggccagtt ttttgactaa aaaaagtttg 540aagagctttc aagaaancta tgatttgncc caaggggccc tccaggaagn ttgttttacc 600caaaattttn a 611 28 443 DNA Homo sapiens misc_feature (1)...(443) n =A,T,C or G 28 cgtgcccaaa gcttggcaag ttttcggctt taaccacgca caccaccaccaccaccatnc 60 taaataactt actgcatcct caaagcctgt tttatgggga ttgcatggttttatttgaaa 120 tcacgcctgt aatcccanca ctttgggagg ccaaggcagg cagatcacaaggtcaggaga 180 tcgagaccaa tctggctaca cggtgaaacc ctgtctctat taaaaaaaatacaaaacaat 240 tagccaggca tggtggcagg cgcctgtagt cccanctact cgggaggctgangcaagana 300 atggcgtgaa acttggaggc ggagcttgca atgagccgag atcgcacttgctgcacttna 360 acctgggcaa caaaacgaga cttcatntct nttttnnaaa nnnaannnnnnnnnnnnnng 420 tcctttggcc cgaccacnct tan 443 29 403 DNA Homo sapiensmisc_feature (1)...(403) n = A,T,C or G 29 ggtacttttt tttttttttttttttttttg gagtgcatat catcacccca acttcggttt 60 tttacatttt aatttgtattgnttttaatt tattttgagg caatgtctca ctatgttgcc 120 caggctggtc tcaaatgaaaacaatgctat caatcacatt cttgcatagg atatgtgtca 180 gtaatcctcc aaaatgaacatganaaatgg aattgtcaag tcatagatta agtgcatata 240 acttttgaat agatagtataaattttttcc ccaaatgaga attttatatt ctcactggca 300 acatgaaaat agccatctctctataatctt atcaaccctc gatagtgtca ttttttaatt 360 tataattatg agtgaaaatggtcctgcccn ggcgggcgct cga 403 30 615 DNA Homo sapiens misc_feature(1)...(615) n = A,T,C or G 30 ggtacagtgg tagcatccaa atgggcaaacgtagtagcag gggcagggtc agtcaagtca 60 tcagcaggca catagatagc ctgtactttgtaatattctt cccacccttg agaatggact 120 ttgtaagatc cgccccctgc ccacaaaaaaatttctccta actccactgc ctatcccaaa 180 cctataagaa ctaatgataa tcccaccaccctttgctgac tctcttttca aactcagcct 240 gcctgcgccc aggtgattaa aaagctttattgctcaccca aagcctgttt ggtggtctct 300 tcacacagac gcgcgtgaca gaaaccacttgaagcccggg cgcggtggct caggcctgta 360 atcccagcac tttgggaggc tgaggtgggtggattacctg aggtcangag ttcgagacca 420 gcctgaccaa catggtaaaa ccctgtctctactaaaaatc aaaaaaanta accnngggtg 480 gtggnnggca cctgtaattc agttcttgggaccttangca ngaaaatcct tgaacttgga 540 ggcggaggtg catanttgaa acaaaccttgnctcaacctg gnaacaaaat aaaaatccgn 600 tnaaaaaana aaaaa 615 31 485 DNAHomo sapiens misc_feature (1)...(485) n = A,T,C or G 31 acgcggggataagctacaac ataaacacat ctaggttctt gttcttagaa tacagcatga 60 agaatttgctttcttctttc ttcctaacat tttcatgtga gatccagaaa ggacacattg 120 tctctggccattcgaagaaa gaaagaaaga aagaaaaaaa aggtatttag agacagagag 180 agaaaaaggctgaaatgggt tcgctgggtt ctaaaaatcc gcaaaccaaa caagcccaag 240 ttcttcttttgggacttgac tcagctggga agtctactct cctttataaa ttaaagcttg 300 ctaaggatattaccaccatc cctacaatag gtttcaatgt ggaaatgatc gagttggaaa 360 ggaatctttcactcacagtc tgggatgttg gaggacagga aaaaatgaga actgtttggg 420 gctgttctgtgagaacccna tnggctngtg tatgtgtgga cagtccttcg gcccgaaccc 480 cttan 485 32780 DNA Homo sapiens misc_feature (1)...(780) n = A,T,C or G 32cgaggtacgc gggtgtctag accttatgtc aaaataagcc caattgtatt aaagagtatt 60aaattgtatt aagaataaaa acacatggcc gggcacggtg gctcacgcct gtaatcccag 120cactttggga ggacgagatg ggcggattac aaggtcagga gattgagacc atcctggcta 180acatggtgaa accccgtctc tactaaaaat acaaaaaaaa aattgtccag ccgtggtggc 240aggtgcctct agtcccacta ctccagagct gaggcaggag aatgatgtga acccgggagg 300canagcttgn agtgagccng agatctcgcc actgcactcc ggcctaggcg acagagcgag 360actctgtctc anaaaaaaat aatgantaaa aaaanaagtc ctgcccggcc ggcgntcnaa 420nggcgaattt cancacatgg cngcngttac tatggatccn actcggtcca anctggcgta 480atcatggcat agnttttnct gtggnaaatg gtatccgtnc aantcnccna attcaaccgg 540agcttaannn ntaacctggg gcnatnnnnn nctacttcat tattgcntnc ntatggcgct 600tncattggaa ctnttgcnct gnntatnatc gcccnccngg aaagnnttnn ntgggncctt 660ctctgttann atctnnggct tngttgggag gntnctntna gnggntngtt tnatnggtcc 720ngnaaatttc agcctangnc antnagcctn ttgnttaatc tccnactnna aaaaataang 780 33742 DNA Homo sapiens misc_feature (1)...(742) n = A,T,C or G 33acataccagg ctgggtagtg aacggggaaa ttgaagcaaa cccgccaaga cctgaaattg 60atgaggatac tcagaccacc ttggaggatt tcagccgaac agcatccgga ggtgcatttg 120tggtatcaca agtcccaagc cttcccttgc ctgaccaata cccaccaagt caaatcacag 180accttgatgc cacagttcat gaggataaga ttattcttac atggacagca ccaggagata 240attttgatgt tggaaaagtt caacgttata tcataagaat aagtgcaagt attcttgatc 300taagagacag ttttgatgat gctcttcaag taaatctact gatctgcacc aaaggaggcc 360aacttcaagg aaagctttgc atttaaccan aaaatattta taagaaaatg cacccacata 420ttataccatt aaaagttnga taaaacantt tgcctcaaaa gtttccacca tggacaagta 480acttggttat cctnagcaat cttgtgcctt gattactcnn ctctattcta tcctgtnaaa 540gcntaatctg agtaaaattt nccctggntt gtggattggc tngtnatgta atttnttaag 600nctggcngac cnctaggnaa tnnccttggg cgttangncc gtngccantt gtattngtaa 660tttctngaat gtnntcnncn nnntaccngt aagnatgggn tnggnnatnn atnttttncn 720tnttnatnnn cntnnannnn tg 742 34 763 DNA Homo sapiens misc_feature(1)...(763) n = A,T,C or G 34 ggtcaaatga ggaataatga ggaaacaaaaccatacatac aagagggatg gcacagacct 60 tgtgacaaag tggtcctgaa atttctggaggggaaatgaa taagaataac cgagatagtt 120 atgcttggag gaagaggaag atcaaggtgtcctaacctac cagaaactaa gacttatgaa 180 accttagtca ttaaaatatg tagtattagttcagaaatag taaataaatc aatgtaactg 240 aatggaacct gggaacaaat atagctacatgtaagatctg ggtatatgct ggaggtgaca 300 taacaaatga agagaaacaa tggactattcaaagctgtgt tgctatcttt attggcaaca 360 aatatgggaa aaaatnaaat gagatcctattcacatgaat gacaaaaata aatgccatat 420 tgattaaacc taaatatgac aaggaaggcctcaaatttta gaaaaaaatg ccaaattnta 480 cncattggga gataattcat taacaagaccaanaaccnta aggaaagatg ntaatttnga 540 tatattaaga tttactatgt ttataaatcaaggatagtcc cgcttaagan actttctttt 600 atttttaatt aatattatta atatttganacttgcttgnt tnggtgaacc ggtaatttgg 660 tattnacctt ctccggttan gattnnctaanccntgtgnt nngttgnncc ncncnatttt 720 tntacagttn ttgcgcgnta ttncnggnngcccccnnngn ngg 763 35 767 DNA Homo sapiens misc_feature (1)...(767) n =A,T,C or G 35 acaggggaat ggaatggaat ggaatgcaat ggaatggaat catccgtaatggaattgaaa 60 ggaatggaat ggaatggaat ggaatggaat ggaatggaat ggaatcaactcgattgcaat 120 cgaatggaat ggaattgaac taacccgaat agaatcgaat ggaatggaatggaacggaac 180 ggaatggaat ggaatggaat ggaatggaat ggaatggaat ggaacggaacggaatggaat 240 ggaatggaat ggaatggaat ggaatcaacg cgagtgcagg ggaatggaatggaatggaat 300 gcaatggaat ggaatcttcc ggaatggaat ggaatggaat ggaatggaatggaatgaaat 360 gcaatggatt caactcgatt gcaatggaat ggaatanaat ggaatggaatggaatggagt 420 ggaataattc naatagaatg gaatggaatg gaatggaacg gaatggaccggatggaacca 480 attgtaatgg aatggaattg atggaatgga atggaatcac cctagtcaanggaatgtatg 540 gaccggattc aatgaatgga tattccgnat ggatggatgg gaatgaattgatgattggat 600 ggatggatca ccatccatga agattgatga tggatgatgc cacccatgatgattatgnat 660 tagngtnata tctncatnna ggatgntncn attatgngnt gatgacatgantannccnnc 720 nctttnancn tatttttttg ggnccccctc ccagttgntt taaannn 76736 608 DNA Homo sapiens misc_feature (1)...(608) n = A,T,C or G 36acatatagtc aacgaaatat tcaaagaata actttatata ctcttgttct ttaaattcta 60tcctctcttt cagaattctt ccatttaagt ttgggtattt tcctagtttc aacagatgaa 120cagaagactt cattgaacat tttgacagta agctactaga gaccaattat caactggtgc 180tacacatgct gtgttatctc ccttactatt aaactataac cctctcttgc tattttgttt 240catgcatcac caaccaaact tcattttttc taataaaaaa taaatatata aagaagacac 300tgacaggcat atattcacaa gatctcaact tcttaaaaca taagtatggg tatatttatt 360tctctcaaat gcatacnaga caataattac ncagcaacca atcttttgtt caacaatgat 420ttgantcata agcatttgga aattacataa tttcatatca atanccctgt tttttnaata 480cagaagtaaa aaanccccaa taaccaatct taaatttcna ttatcccctt acctccaacc 540tttnaaaggt cccaccgggc cttttccnac attaatttgg tnaaactggg gttnaaaacc 600gcctnccn 608 37 245 DNA Homo sapiens 37 acagacatgg cggcggcttt tcggaaggcggctaagtccc ggcagcggga acacagagag 60 cgaagccagc ctggctttcg aaaacatctgggcctgctgg agaaaaagaa agattacaaa 120 cttcgtgcag atgactaccg taaaaaacaagaatacctca aagctcttcg gaagaaggct 180 cttgaaaaaa atccagatga attctactacaaaatgactc gggttaaact ccaggatgga 240 gtacc 245 38 630 DNA Homo sapiensmisc_feature (1)...(630) n = A,T,C or G 38 actacactga attcacccccactgaaaaag atgagtatgc ctgccgtgtg aaccatgtga 60 ctttgtcaca gcccaagatagttaagtggg atcgagacat gtaagcagca tcatggaggt 120 ttgaagatgc cgcatttggattggatgaat tccaaattct gcttgcttgc tttttaatat 180 tgatatgctt atacacttacactttatgca caaaatgtag ggttataata atgttaacat 240 ggacatgatc ttctttataattctactttg agtgctgtct ccatgtttga tgtatctgag 300 caggttgctc cacaggtagctctaggaggg ctggcaactt anaggtgggg agcagagaat 360 tctcttatcc aacatcaacatcttggtcag atttgaactc ttcaatctct ttgcactcaa 420 agcttgttna gatagtttaagccgtgcata aattnacttc caaatttaca tactctgctt 480 anaaatttgg ggggaaaaattaaaaaatnt aattggccag gatnttggna atttgttata 540 atgaatgaaa cattttngnattaaaaatca nattacttnt aanctttgat aaantaaggc 600 atggntgggg gtaattgggttttttgttcc 630 39 626 DNA Homo sapiens misc_feature (1)...(626) n =A,T,C or G 39 acagtggtcc ttttcagagt tggacttcta gactcacctg ttctcactccctgttttaat 60 tcaacccagc catgcaatgc caaataatag aattgctccc taccagctgaacagggagga 120 gtctgtgcag tttctgacac ttgttgttga acatggctaa atacaatgggtatcgctgag 180 actaagttgt agaaattaac aaatgtgctg cttggttaaa atggctacactcatctgact 240 cattctttat tctattttag ttggtttgta tcttgcctaa ggtgcgtagtccaactcttg 300 gtattaccct cctaatagtc atactagtag tcatactccc tggtgtagtgtattctctaa 360 aagctttaaa tgtctgcatg cagccagcca tcaaatagtg aatggtctctctttggctgg 420 aattacaaaa ctcaaagaaa tgtgtcatca ggagaacatc ataacccatgaaggataaaa 480 gccccaaatg gnggtactga taataacact aatgcnttaa gatttggtcaccctctcnct 540 aagggagccc attgagccna nggngctaaa gcctcatact ccacctgaatggttaggaga 600 aaatttatcc caaaaaaaaa aaaaan 626 40 645 DNA Homo sapiensmisc_feature (1)...(645) n = A,T,C or G 40 cgaggtacgc gggcaggacatttaaaaggt ttcagcagaa atcttatgat tatgtctgac 60 ttgcagtatt ttatttgcctctttgacggc tttttttttt tttttttttg agacagagtc 120 tcacactgca ctccagcctgggtgacagag tgagagactc cgtctcaaaa atgaatgaat 180 gaatgaatga atgaatgaacaaacgaacaa ggtggtttaa tgtcagaaaa cttcctaagc 240 atttgctccc caaacctttcatgtttttca agaagccttt attacataaa ggggaataga 300 attaaaatgt ttctttataagaaaaatata catatttgtg ttcttggccc cattaaaact 360 aatcagtagt cctttggccaaaaaatagtc aacaaganaa ctgggtatga ntccnggcnt 420 tactcctgnt cataagtgnggatgcntgtg tctganccna actgnctcaa ctngagctct 480 tggggtataa caanaaacccgngttttcat gaaacccctg ggccnttata aaaggtttcc 540 cttggggggc ccaatgcttattntngattn gggttccaaa anntngcaat tggnataggt 600 gcttgaaata acccccttttagtnnaattc cnaccaaaac cntgn 645 41 616 DNA Homo sapiens misc_feature(1)...(616) n = A,T,C or G 41 acgcggggct cttcacgagg tggaaacaagatggaggatt cggcctcggc ctcgctgtct 60 tctgcagccg ctactggaac ctccacctcgactccagcgg ccccgacagc acggaagcag 120 ctggataaag aacaggttag aaaggcagtggacgctctct tgacgcattg caagtccagg 180 aaaaacaatt atgggttgct tttgaatgagaatgaaagtt tatttttaat ggtggtatta 240 tggaaaattc caagtaaaga actgagggtcagattgacct tgcctcatag tattcgatca 300 gattcagaag atatctgttt atttacgaaggatgaaccca attcaactcc tgaaaagaca 360 gaacaagttt tatagaaagc ttttaaacaagcatggaatt aaaaccggtt ctnaagatat 420 ctcctccaac tctaaanaan gaatataaatcctatgaacc aagctcgcct tttaacagtt 480 tgattcttcn tactgatcca aaataagcggttttacctcc ttattgggag acattnttta 540 aaaaagaaag tccatntntg naacctttttccaaaatttn tcagananac atgctgnttg 600 gngacggctt aaaatt 616 42 259 DNAHomo sapiens misc_feature (1)...(259) n = A,T,C or G 42 ngtacggtcggtggcagtgc tattctgaga tctgtagatg cttagaatat cagtattttg 60 gatgttgctgcattttacaa tttatttgga gtcttccttn attttcctcc agatatatga 120 aaatatgcaatacctgctta tatcatgtag aaaagcttag caattattaa tttttctnta 180 tttcattttatttgaccaaa gtcggtgctt cacttgactc antgtgtttt aggtgttngt 240 ntttntacctttccggtca 259 43 509 DNA Homo sapiens misc_feature (1)...(509) n = A,T,Cor G 43 acgagtgtat ttttgatggg aaggccatgc taaatctata aaacagatgtttcctctccc 60 aacagtggtc accagtagtt tcaacttttt ccccccagta gcatcaaccaaacttagcat 120 agtgattttt aactctttgc tcccacacgc actcatccca acttccccgcttgccccact 180 ccctgggggg aaataaccct gcctttaaaa taaatagcaa ccaagtgctcagttctatgg 240 aaagtatgaa tatttatttc aggctttcga tcccaatcga tttcaaaaaacaaagtctga 300 tttctctcct cagagcagct gaggcctcca tgttacgatg gtttcatggagattgaagga 360 gcacatttca tcaggcttag cacaaagtcc ctgatgccca ccatgtcccagccttagnaa 420 aggaaagaaa cagaattcac caccatgggg ctgaacgaat gccacacctaatgtaaatga 480 ncagctaacc ttggccaaat tgtggtttt 509 44 544 DNA Homosapiens misc_feature (1)...(544) n = A,T,C or G 44 ttttttaaaa gtgtcactnantctttaann anatncatta ccattttttt tncaaantaa 60 attacggttt taaanggaanacacatggna atntananaa ncaccgnnga annttaanta 120 cctngggngc gancanactnanggcgaatt cgaaccaatg ggggcngnaa cnaggggatc 180 ccagctnggt accaaaattggcgtnatgat cgcaatagcg gtacctgtgn naaanggtta 240 ttcnntngta aaancaganntcntnnaagn nngaccaaaa aangtaaatc ctggggtgcc 300 taatgannga tntaaancnattaattgggn tgcccacctg cnantttatc gttcaaaaac 360 ccgttaaacn ngtgnaaaaatgaatngcca acccntngga aaagccgnat cntttgggng 420 cttttccttt ttggtcctnacncttcctan nngnnngttt gggnncggnt nagttcntaa 480 aggcgnaaaa catttacaaaaataggggaa ancccgaaaa acatttaccc nagccacctt 540 ntcn 544 45 630 DNA Homosapiens misc_feature (1)...(630) n = A,T,C or G 45 ggtactctct atcactgacaaatgcaggct ggattcttat tatatacaga gatggctcaa 60 aaatggggtt tcagatctttgtgacgaaat agaatactgt ttcatatttg aatcagaggg 120 cttcttgttc tgagaaataggttcaaaatc attggaacca ggaacaagaa tagcttattg 180 ttatctgtga taacactgttttctaaacac aaggattttc ttttttatta atatgcaaca 240 tagacattgc cataacagaataataaacca catgtggggt tttaaaaatg aaatttggct 300 aataggagca attcagctatttttctatca agaaattggg tggggtggga tagaaagaaa 360 aaccgggttc aaccccacttctgcccccta accagctata tggcctggat ggagcattca 420 acctttaata agggtcaatttcntctgttn aaaagacccc aaacctggaa atcacnttng 480 cctctccctg aaaataanaaggctngattt ttggaataan aaacataatg nangctnggc 540 ccaatggctc gccccgtaatccaccctttg gaggccangc ggncggacac ttgaggtagg 600 agttgaacca cccgccacctgggaacccnn 630 46 622 DNA Homo sapiens misc_feature (1)...(622) n =A,T,C or G 46 tttttgactc ccaaagtcat tttatttaac aaaggggtca aggcagaggaaagtttccct 60 taatatcccc acaactgctc cacatgtctt ctgtggaaac acttcaccaggaactagctc 120 aacactcttg ctaacaattt agtgtctata caggaaggct ggtgtctctgttacaggtgg 180 cccgttcctt aaagccttta gggttaatcg cagctgcact gagtggccaagcagaccctg 240 ttgggatgtg aaagcagttt gttaacaggg cccctggccg ggcccagaggctgtcagact 300 cancaagtaa cactgaatgt ccaaaaatac ggctgtgtta aactaacaagccaatccttc 360 tgctcagatc tctggataga aatgattttt cttttatcta tgggggaatgcaatttcatc 420 acaacccctt acataaacgc tcctgaaacc ctttcagtag acagcatttcaattcaaaaa 480 ccaaaagtga aactatcttt gaaaacangg acctggctgg gaaaccatgcacacctcggc 540 gaacactttt cccccccacg aacttggact ttntgggaag gtggcgggtttttggcnaaa 600 acattcttga agcntaggaa gg 622 47 253 DNA Homo sapiens 47ggtactttgg tttgaaaaca acacttagag cctccagata acttttaaga cttatttagc 60tttgtgggtg gtattttcat gcaaataagt aagggtgggt tttatatttt gtagaagttt 120tcggtcctat tttaatgctc tttgtatggc agtatgtata tattgtgtta agttcctcaa 180gaatctcctt aaaaactttg aagttaatac ttttgtgcaa ctgtgttttg aataaagcca 240tgacagtgtt aaa 253 48 607 DNA Homo sapiens misc_feature (1)...(607) n =A,T,C or G 48 acttacatat cctacatttg actacattat ttccaaacca agtattccatccaaaggaac 60 atactgctat catagagacc aaggagggac tgtttaaagt tgccaaggtgaagcgagctg 120 agaggctttg tcctcgtgcc agtaactctg aaatttctct taattcctgctgtccaggca 180 gcagaatgcc atggtttccc caagtaggta gctgctttag cagttaaagcccaaatgtct 240 gttctgttga tcaagaggtc tctgaatttc tgaagtggtg tttcgtttctggtgactgag 300 ttaatccttt acaatncctc ttgtaaagtg tgctaataga aagaatccacctttcaaagc 360 tgcagaacca naccgtgccc taaattgacc aaccgtanct gatgtgcctnangaagtctt 420 ttgccaactg ccctgtgaan acccctnctt ccccccagct ngtggcttgcacactgaaca 480 tttaaactgn gcaaagccgt gtagttataa nacagtaaat cccaaggcttggttaantgc 540 tgggnnaaaa ctggttggat anacttaact taaaacccct tacataaacntnggaactcn 600 aagaaaa 607 49 421 DNA Homo sapiens 49 ggtaccactggatgaggggc cgggacatac tgactgcccc tttgacccca caagaatcta 60 tgatacagccttggctctct ggatcccttc tttgctcatg tctgcagggg aggctgctct 120 atctggttactgctgtgtgg ctgcactcac tctacgtgga gttgggccct gcaggaagga 180 cggacttcaggggcagctag aggaaatgac agagcttgaa tctcctaaat gtaaaaggca 240 ggaaaatgagcagctactgg atcaaaatca agaaatccgg gcatcacaga gaagttgggt 300 ttaggacaggtgctgttccc gagactcagt cctaaagggt ttttttccca ctaagcaagg 360 ggccctgacctcgggatgag ataacaaatt gtaataaaag taacttctct tttctttcaa 420 a 421 50 624DNA Homo sapiens misc_feature (1)...(624) n = A,T,C or G 50 ggtacttcagtattgcattc tattcctctt aatgttttta tgggatctcc agggaaagag 60 gaaaatgaaaaccgtgatct aacagctgag tctaagaaaa tatatatggg aaaacaggaa 120 tctaaagactccttcaaaca gttagcaaag ttggtcacat ctggtgctga aagtggaaat 180 ctaaatacctctccatcatc taaccaaaca agaaattctg agaaatttga aaagccagag 240 aatgaaattgaagcccagtt gatatgtgaa cccccaatca atggatcctc aactccaaat 300 ccaaagatagcatcttctgt cactgctgga gttgccagtt cactctcaga aaaaatagcc 360 gacagcattggaaataaccg gcaaaatgca ccattgactt ccattcaaat tcgtttattc 420 aaacatgatcaagaaacgtt ggatgacttt aaaaaanatg ccntaaggac anttgtgatt 480 tgcaggtgggaagatnaaca gttcatatcc actgaatgaa atgcatcttg tggaaganct 540 catgnatnaaggttaatggc tgaaatgaaa actccaaaag aaaccaaaaa ataccggccc 600 ctttgaaattcaggganncc tatg 624 51 632 DNA Homo sapiens misc_feature (1)...(632) n =A,T,C or G 51 ggtacgcggg ggaaacggaa gtgagcggcg gggtcgactg acggtaacggggcagagagg 60 ctgttcgcag agctgcggaa gatgaatgcc agaggacttg gatctgagctaaaggacagt 120 attccagtta ctgaactttc agcaagtgga ccttttgaaa gtcatgatcttcttcggaaa 180 ggtttttctt gtgtgaaaaa tgaacttttg cctagtcatc cccttgaattatcaagaaaa 240 aaatttccag ctcaaccnaa gataaaatga attttttccc cctgaagaaacattcagggc 300 tattttgctt cccttaaaat accagaatgg gattcaaggg cagtgccaccaggtcaaccg 360 ctttcatttc tttcaagcct caaatctttc acttgaatgt ttgaagggtaatggatgaag 420 acctattgga attgagggat atctttaatg atccgcccca aaccgaatccttggaaaagc 480 cacccttgat ggtggaatat aaccttggtt actgaatatg tgcctgtcatggaaccgagg 540 ccgcatctgg ttatagcatc tttgacctgc cggccgcccc aaaggcgaatccacncctgc 600 ggccgttcta tggaccaact cggnccaact gn 632 52 623 DNA Homosapiens misc_feature (1)...(623) n = A,T,C or G 52 acttttaatg gtgggaatttacagtagaag catcctttgc tgagttatac attcctttat 60 caatctcttt tgatacaacatttaaaacaa gtagcttcaa gaaaccactg gtgttttgag 120 gatagtattt ctaaatagcattcaggaaca gagtattatt gcacagatct gaagatcaaa 180 aaaaagctca aggaaatacagatcggaagt gctgatgagt tatatttatt gaaaacccaa 240 cttttaagga agtgctaagatcagtcaccc atgtgaataa gaagccagga aaggaaagat 300 ggggaaagcc canatcaccaggcttctatt aaggaggaaa gcaacagang aaacagtgaa 360 agggaacaga aaggggtagccaagtgttac aaaaaanccg actggataac caaactncaa 420 aaagngtatg ttggggagaactgaaangga aaacaaaata cttgactaat cntaagtaga 480 aaaaagcagn tagagaaaaccaaatatttc tggncctgtc acatacaact tcaaataccc 540 ttatanaatc caaaaatgatgtgtgtaagg naaaatttat tgccntccga aaaataantt 600 tntccaatnt gaaacaaatcaac 623 53 627 DNA Homo sapiens misc_feature (1)...(627) n = A,T,C or G53 ggtacgcggg gtcgcatgcg ctgtggctaa tgccgtaggc tctttcaggg ctgagccatc 60ctgcgtgtct tgcgctcggt ggaaatgccc agccgaggga cgcgaccaga ggacagctct 120gtgctgatcc ccaccgacaa ttcgacccca cacaaggagg atctaagcag caagattaaa 180gaacaaaaaa ttgtggtgga tgaactttct aaccttaaga agaataggaa agtatatagg 240caacaacaga acagcaatat attctttctt gcagaccgaa cagaaatgct gtctgagagc 300aagaatatat tggatgaact gaaaaaagaa taccaagaaa tagaaaactt agacaagacc 360aaaatcaaga aatagtcaac ctgatttcac ataacaatgt gtggcatttg ttgttctgta 420aacttttctg ctgagcattt cagtcaagat ttaaaagagg acttactata taatcttaaa 480cagcggggac ccaatagtag taaacaattg gtaaagtctg atgttaacta ccagtgntta 540ttttctgntc acgtnctaca cttgangggg gtttgactac ccancctgtg gaagaagaaa 600gaagcaatgn ggttctatgg atggaga 627 54 565 DNA Homo sapiens misc_feature(1)...(565) n = A,T,C or G 54 ttttccttga gtgctccctt ttatgtcattttattttctt ttatgcagac cagtgggggg 60 aaaatcccat agattcttct ggaaactgtcaagatgctgg gaagatgaat gcaaaactta 120 catagattgg gatgtccaca gtttggattttcaaggtatg gcttttgcag gatgacgtga 180 tcaacccaaa cttctgcttg atctggtttgtcctgaactc ctgccacttg ccgccaacca 240 gggcctctgc tctgatctca tacttcaccaggcgtgccgn tcgcaggctg acgtggttgt 300 gctcgtagac cgcagaggga gattccaggtctgtgtgctt tattctctgc atgtaaaaac 360 tataagaggt agtatcatgt ttgagtccctttatcttaaa gaagaatcca tatagagcaa 420 tcgttttcga ataagttgna ttctctgngtctggcactgt gtccagtgct ctcanaggat 480 gcangggaga anaccaaaaa gtntctgagcagtctcacat gggaaataaa atgtgtcccc 540 ggtaccttgg ccgngaacac nctaa 565 55451 DNA Homo sapiens 55 acagagatga caagagaaag gcacaaatga ccggagtcagggattgtggt gagggctcca 60 catgaagaca gcatgttgga ggagaccaag ttgggaagggtgacatgtca tacatcaaaa 120 gttgccccaa gatagcaggt tataatgggc tagagagaaattagagggaa catctcttcc 180 ttcacttgaa caacaccaaa aatagaagac cagagaatagaaggatggtg acaaatccca 240 aaaaggaaat ggaggaggag ttcgtggaag ggcagaaacactttaatcct agagggaggg 300 tgaggcactg ttgaaaagag aagcaaactt tggcaggggtggccattctg ccttgctgag 360 tcatgggctg agatacggaa gtcactttca atcattttctacttctccca gggcactcag 420 acaaaatcag tgcaaggtat atggaagtac c 451 56 623DNA Homo sapiens misc_feature (1)...(623) n = A,T,C or G 56 ggtacgcggggcttccgaga cgcactgggg gccggatgta gaatcctgct tatctgtgaa 60 atgcagttaacacatcagct ggacctattt cccgaatgca gggtaaccct tctgttattt 120 aaagatgtaaaaaatgcggg agacttgaga agaaaggcca tggaaggcac catcgatgga 180 tcactgataaatcctacagt gtttcactct tgttgcccag gctggagtgc aatggcgcga 240 tcttggctcacggcaacctc tgcctcccgg gttcaagcaa ttgtcctgcc tcagcctcct 300 gagttgctgggattacagat tgttgatcca tttcagatac ttgtggcagc aaacaaagca 360 gttcacctctacaaactggg aaaaatgaag acaagaactc tatctactga aattattttc 420 aacctttccccaaataacaa tatttcagag ctttgaaaaa atttggtatc tcaacaaatg 480 acacttcaattctaantgnt tacattgaan aagggagaaa acnataaatc angaatacct 540 aatatcttcagtngaanggc atcaaggttc tcttgaaaac ttnccggaat aatgaatntn 600 ccnaagtccaaaanattttt aac 623 57 622 DNA Homo sapiens misc_feature (1)...(622) n =A,T,C or G 57 cgaggtactt tttttttttg tttttttttt tttggtttct gtcctttaattttttaacag 60 aatatacaga gccacacaat acgatttcaa tttcaaatta tgggagatcatattcaaata 120 tgcttaggtt tgacaagttg ctgttacaat actgagaact ttcatgaaaacggtatttaa 180 caatttttaa gataatcaaa tatctttttg ctacgtgggc caacgcattaatactaactt 240 gtttaaaaat gcagtctttt agacttcaaa ttattataaa acaatatcaagatcatatag 300 atatacttcc tgattactca aaactcgttc cattctgatg gaggctgaaggtaaatgtta 360 ttatacatta gaacatttca tgaaaccact tctcctttgc acttacctgtaaaagtcaaa 420 aattaaacca caatttccta agacataact atttctagaa tacattggtgtaatcataaa 480 agactacnag taaattatca tttttatcta acacttttta ccacacacatctttcctaaa 540 aggaccnaaa aaaattggga atttggattc cttacataac aggactcatacttctgattt 600 aataaattnc actcttttca ag 622 58 471 DNA Homo sapiensmisc_feature (1)...(471) n = A,T,C or G 58 ggtacttttt ttttgtttgttttctagact taataaaagc ttaggattaa ttagaagaag 60 caatctagtt aaatttcccatttgtatttt attttcttga atactttttt catagttatt 120 tgtttaaaaa gatttaaaaatcattgcact ttggtcagaa aaataataaa tatatcttat 180 aaatgtttga ttcccttccttgctattttt attcagtaga tttttgtttg gcatcatgtt 240 gaagcacccg aaagataaatgatttttaaa aggctataga gtccaaagga atattctttt 300 acaccaattc ttcctttaaaaatctctgag gaatttgttt tcgccttact tttttttctt 360 ctgtcacaat gctaagtggtatccgaggtt cttaatatga gatttaaaat cttaaaatgn 420 ttcttatttt cagcacttacatcatttggt acctgccngg cggccgntcg a 471 59 618 DNA Homo sapiensmisc_feature (1)...(618) n = A,T,C or G 59 ggtacataca caatcactcaactggaacaa tcaaaaccat ctatgagtgt ggttattaaa 60 aaataaaatt acgttcatacaatggtagaa aatgaaatgt ttttattaat ttgattatta 120 atacaaaacc acacatatatgaattatata acctagtgtt atatatttaa aaatctttat 180 gcttgcaact gaaatgtctctactccaagg gaagtttctg atttttaatt ttcttatttt 240 aaggaatcta ttatattcacaatgattaaa atgccttaca cataggcaaa aagcagaccc 300 aatcccagca aacagaaaaaccataagtct atcatatcac catatgtttc accatatagt 360 tttgaaaaat aatcctatttgcagtttggt atgtcttcat atttatactt attatcaaag 420 tgattgcata ttgaggcacagagcttaaag aggaaatata tattacttat aggggaacca 480 gacactgaaa caaggaatatcaatcaatgg cttcaaacna aaaaaaaann nnnnnnnnnn 540 nnnnnnggaa aaggaaaagtcctgncccgg cggncgttca aagggcnaat tcaaccactg 600 ggggccgtac ttatggac 61860 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 60actttttaaa ccctcccaac cagccctttc tcaatattca tcaaatctaa aacatttagg 60gggcaaaatt ctaacatgtt catggtatct tgcaaatagt aaaagcttta ttctgaagga 120ttataaacta gttttctcca ttttaactag cactattttg tggaaattag aaacctcttt 180tatttctctt cccaaaagta atacttatta taaggctgta gtatcaggtt aaggatacag 240ataaataaag ttcacttata tcttcttaca aatgtctggg ttttaatatg gttaatcact 300tatatacaaa tattacaact ttttagtgca agtttttgga agaaaacttt ttgataaaac 360actgtgattg atgtgacttt atttttaatt taaacgatga ggtggccaga agaaagatgg 420gtctaaaatt tctcccatga aagatgtaaa actatggctt ttttaaaatc aaaatttcat 480ctttaaaata atgggttgaa atctggatng gatctgaaca gaataatcac atttaggatc 540tatataaatc tcaactggag tntaactgaa ggaaataccn ngattttaag aaatatnttc 600aaaaan 606 61 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C orG 61 ggtacattct ggtatgaaaa catctcaaaa tgtaacaaca caagagtttg ggtcaagacg60 acccacccag gaggctgtaa aaactggttt gaactagaac tgtggaatgg aactagttta 120aaatatgaag cagctctaaa caccaagctt agagacattt gccctattag aaaacaaaaa 180tcattaaagc tacaaaataa caagtgcaaa catgctgaac ctgtttccag ggagtgacat 240tcccttctgc caacaggtcc caaactcaca cccacaaggt gtaactctct ttcctgttcc 300actagatttc ctttctctca tctcaaaggt cctcagaaat gacaatggaa aacgtatgaa 360ttgttgaaat ttaccctgtg gaccaattcc tgaagagata acagccacaa ctctgagatg 420attaagacat gcagtgttta cttgatgact ttctgnattt ctagaaaccc tcaaagcatt 480aaactgncta tttcaaaatc taaacttnct agcactttta ttatttggag taagcnnacc 540gaagacaatt tactggccca caggaataac cacgcttact tgtcaccata agtttacggn 600atggacattc actggaaaac 620 62 614 DNA Homo sapiens misc_feature(1)...(614) n = A,T,C or G 62 gccgaggtac ataaatctgt gatcccatttcttattgcac cattcaggaa cactttatat 60 aaatgagtgg ctttttattt catattattagtagtatcat ggttccatta caggcctatt 120 aacatcatac attgtcatta gtctttgaagaaaaaatatg taaatatata tgtgtaacat 180 gagaatttct ctctaaagca gggcttaaaattttttggaa aagtttgaca aagcatacca 240 catgaattca gatttacctc aatgctaagaattatgttta gttaggaaaa aggaaagtca 300 ttttgacctc aggtagaaaa atagattgctttgagtttta tgtagcttta gactttaaaa 360 agttagaatt tattctgtaa ctaaaaattatttgaaaaaa ttatgcctct ggtttaatta 420 ttggtgatta cacactcttt ctcttacccttgngtattga actatgtcca taatcaagtt 480 gatgtggatc ctgaaaaatg gtatgaacatctgatgggat tggcacatta ttttaaaant 540 agcatctgac acttcaaaac tgtcantgngatgggttcac cataccacgg ntgaccntac 600 attaaatttt nacn 614 63 616 DNA Homosapiens misc_feature (1)...(616) n = A,T,C or G 63 ggtacatata agagtaattagttttattct ctctttttta taaaatcggg tttcagatga 60 gatgtttatc ttagactattttagggaaaa attttacatg tttgagatgg tggagtaaaa 120 agactgttaa acatttcttttaaaaaatta tttttacatt acaacaatat atttatgatg 180 tgttcagatc aaaaatttaacttctgtgtc ccagatctac tttcaaagtg agattttcac 240 ttgtcagctt aaatttctgactagaactaa catttgtgta tttttgtgct tagtcggaat 300 acaaatttca cagtggatttttgaagtttg tccttaaatt ggataaaatc aagtgattaa 360 agttactaaa gagataaaaatggtaatttc catttttaaa agtaatttgg ttgtgtttat 420 agttatttgt acttcgagtctcccttcacc atttccgacg gcatctacng ctcaacattt 480 tttggtaccc cangctttcacggacttcac gtcattattg gctcaacttt cctcactatc 540 tacttcatcc gccactaatatttcctttac atccaacatc ctttgacttt naagccgccg 600 ctgatnctgc attttn 616 64612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,C or G 64ggtacagata tcattncttg tgtatgccat gacttgaaaa agtttgggaa gctctttanc 60aatatcagct aanaggatat gaaatcacag gtgatagcag ttgtcattca gtaatttcct 120acaagcagca ccccaaagga aatatagtcc taatctttac tatccacttc taaatttaat 180gtgaatttca tacatgttat tagttgtttc ctttataatt ttataaaaat tattcatcgg 240gagtttaact tccacttcca tgctatcgga tgtgttgggc tccatgcaag aacttggaag 300aaaaacaggc aggaatgcat ttgcataatg acccagatca tcattttctg caactgagaa 360ttatatttca tcattgcttc tagaagtctg caattcttta cttttctttg gtgcattatt 420atctangtgc ccatcactgg ataatgtgga gtgactagag aagtcatnta tcactggaag 480gncctgcccn ggcggccgtt caaaaggnca antccagcan nctggcggcc gttctaatgg 540gntccaactt ngggnccaan cttggngnan tcatggcnta acnngttccn ggggggaaat 600gntntccctc ac 612 65 599 DNA Homo sapiens misc_feature (1)...(599) n =A,T,C or G 65 acaagctaca aaatagcatc tctttcatgg tatgtttgag tgtgtaattttagtttcttt 60 tctggttgta tttgtggtag tcagatgtgt tggattgatt ccaactggacagagtaagga 120 attccagcat cctcttcctg cttgctcgtg ttaccccaca gatcaaaccctcaattctag 180 ttggggatgc tgtctagccc cacaccatga ctgaagcctt aagcactgttgcgcctccat 240 gtgctttggg tcagcaaccc cagtggtatt ctaccagagc attgtgggaaggcagatgta 300 tagtcaggtc ccaacagcaa attgttgggt gtgagagttc taaagtataggggtgaaggg 360 aaagagaang atatgaactc ctctgacctt aaccacattc atttaacttttatgcctact 420 taacaagaga acctggagaa aactatcgna ttcaagagat taatcaaaatcagggtttan 480 ccagccatga ccgaaancnc cttccttaac ctcatcttgn anggctgnaanaattcannc 540 ctaggatggt taanccagaa ccccngatga ttaantgtcc aaccttnatttncatantn 599 66 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,Cor G 66 ncatgacctt tagtggaaga ttatttggtc atcaaatacc catatccaagtttccatggg 60 gcctgggaat ttcctttcac ttggatagaa agtatatatt aggaaagtccagttaataag 120 tatttttatt taaaaaaaaa aaaaaaggaa aaaagaatca gcagaagtcaagttgtctta 180 agtcttaagg ctttctggat ttcttccttg gaggaggtca ggatcttcccaaggcctggg 240 tcctcgaata ttcttccagt catcaaactt ggagtctttg attttctcatattccgactc 300 taaagatatt ttattctctt tcagtttttt ttcaagctca ggatccattttactcttcac 360 agcatcatat cggatttgag aaaactcacg aagaccaaaa gaaccttcaacaatcagcaa 420 caacatgggg actccatacc cagagtcttg gtcttgcgaa aagcacgcntnaaccgcggg 480 tgccaacatg agtgaactct ttcatcggtt naaactccaa cnggcctacgcaaactccca 540 atttacaggt tangctttta ccaaacaagt ncctnggcgg gacnccctaggggaattcgc 600 cactgggggg t 611 67 639 DNA Homo sapiens misc_feature(1)...(639) n = A,T,C or G 67 nagaattcgn gcttncnagc ggtcgnccgggcaggtacac tttacttaaa aactattaac 60 agtttttcat gttgcactgg tggtaattttgaacttggaa ttactgggtg ggaattccag 120 gaaccacaga gtattgattt ttgctgccaaaatgctcttg aagcagatgt ccctgtgctc 180 ccctggctgc ttctggctga aggggggaggtgtagactga agcttgggca ctcatgtgtg 240 tcccctccca gtccccatcc tagtggggccagtctcatta ggcagccata gataagcctg 300 gaacttggct gcattagtga cttgatcctggtatgaaatg catactgggt ataaagntgc 360 tcaagnattt tatttccttg gccacaacttccatagatgc caatggtttg atagcctcag 420 tttctnaacg atgtcttttg gttacagtgctcacttantg ngagtcaaga aatgcttgag 480 ttaccagaaa cttcttantc aggttgagtaacnttttacn ttcatgngta nctnnggcgc 540 gaacaccctt anggggaatt ccacacacttggnggccgta ctaanggatc caacttgggn 600 ccaacttggg ggaaaaangg cnaantggttccttgngaa 639 68 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,Cor G 68 tcgaccggcc gcccggccng gnccttcccc atcactnnac tggnacnatcaaaaccntct 60 atgantgngg gtattaaaaa ataaaattac gttcatacna tggtagaaaatgaaatgntt 120 ttattaattt gattattaat acaaaaccac acatatatga attatataacctagtgntat 180 atatttaaaa atctttatgc ttgcaactga aatgtctcta ctccaagggaagtttctgat 240 ttttaatttt cttattttaa ggaatctatt atattcacaa tgattaaaatgccttacaca 300 taggcnaaaa gcagacccaa tcccagcaaa cagaaaaacc ntaagtctatcatatcacca 360 tatgtttcac cntatagttt tgaaaaataa tcctatttgc agtttggnatgncttcatat 420 ttatacttat tatccaagtg atgcntattg angnccnaag ctttaaganggaattttntt 480 cctatngggg acccnaccct tgacccgaat tcatcaangg ntttaacccaaaaaaaaann 540 aaaaaaaaat ggnaangggg ctcccttnaa anccccccca acctntttntttaacnagnc 600 tnagcctttc a 611 69 606 DNA Homo sapiens misc_feature(1)...(606) n = A,T,C or G 69 accaaagcat tacccgcatg gtagagaacacactcgatta aaaatgttaa gctatctgaa 60 aaataaaatg tgcaagtctt caggatggcacaaaacaaag gtcaatgctt cttggggcac 120 atttcttaga gggcttgctg agtgtgtaaatataatcgac ttttgtttgt gttacatgac 180 ttctgtgact tcattgaaaa tctgcacaattcagtttcag ctctggatta cttcagttga 240 cctttgtgaa ggtttttatc tgtgtagaatgggtgtttga cttgttttaa cctattaaat 300 ttttattttc tttcactctg tattaaaagtaaaacttact aaaagaaaag aagtttgtgt 360 tcacattaaa tgggtttggt ttggcttcttttaatcaggc tttctgaaca ttgagatatc 420 ctgaacttag agctcttcaa tcctaagaatttcatgaaaa gnctntnact ttgaacccaa 480 accanaatac ctcggccgga caccctaaggcgaattccag ccactggcng gccgtactaa 540 nggatccanc ttggtnccaa cttggggnaacatggcnaac tggttccggg gaaatggatc 600 cccncn 606 70 611 DNA Homo sapiensmisc_feature (1)...(611) n = A,T,C or G 70 ncgtggncgc ggccgaggtncttttttttt tttttttttt ttttttttnn aaaangggta 60 accttaaagg tttantggccccccaaangn aacctggggt taatggcttc nnattttaaa 120 tttttggaaa ttaaaaaaattacnagtttt aaatagccna tggctggnta tgttttcana 180 aaacatgatt agactaattcattaatgggg gcttcaagct tttccttatt ggctccanaa 240 aattcacccn ccttttgncccttcttaaaa aactggaatg ttggcatgca tttgacttca 300 cactctgaag caacatcctgacagtcatcc ncatntactt caaggaatat ccgttggaat 360 acttttcana aagggaatgaaagaaaggct tgatcatttt gcaagggccc caccacgtgg 420 gcgganaaat cacttctacaggttattacc tgganngtca aagntttctg naaaacanct 480 tgctctcaac tggtttaccatttggtgctg gagctnacaa ccggtttaag gcccttggna 540 anggtccaag ncccaanaaactttcccggt ccttccggng gccttnaagg gaatccnccc 600 tgggggcgtt t 611 71 588DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G 71 nctgggaacnccgaaggtgg aaggccnttt cataacattt cttgtggatc aaaccaccgg 60 gacaccttttttnccatcaa caggactagc gtcttgtcag tcttggtgac agtgacattg 120 aangtgggggcccaccggtg ctcttggtac tttcccaaga ggtcctcatc ctgagacggt 180 ctctacccatgtttaaccca aagagtgcag gccaggttcc ttatccttct gatgaaggat 240 gagagaactcatttagaagt cagagcaaac tagggtctca gtattgagaa acgcacctgc 300 canggaatcacagagacatc ggggtgcccg cgatggcctc atgaaccatg cctngacggn 360 attcaggaaccctgcaaacg tgctttttga ctcattggnc agtgtgaatt ttacacaagg 420 naaacctggtcnaaggcatt ngggaattgc tccaacnnat acttcctntt aggaacccaa 480 ggaancaggttcncgaattt tgaaaactgg gtntgaagtt ctttcttcct ttgggnacaa 540 ggccttaacaaanancttgn ggnttccaaa tggncctggc cccacacc 588 72 591 DNA Homo sapiensmisc_feature (1)...(591) n = A,T,C or G 72 ggtacaaact tagaagaaaattggaagata gaaacaagat agaaaatgaa aatattgtca 60 agagtttcag atagaaaatgaaaaacaagc taagacaagt attggagaag tatagaagat 120 agaaaaatat aaagccaaaaattggataaa atagcactga aaaaatgagg aaattattgg 180 taaccaattt attttaaaagcccatcaatt taatttctgg tggtgcagaa gttagaaggt 240 aaagcttgag aagatgagggtgtttacgta gaccagaacc aatttagaag aatacttgaa 300 gctagaaggg gaagttggttaaaaatcaca tcaaaaagct actaaaagga ctggtgtaat 360 ttaaaaaaaa ctaaggcagaaggcttttgg aagagttaga agaatttgga aggccttaaa 420 tatagtagct tagtttgaaaaatgtgaagg actttcgtaa cggaagtaat tcaagatcaa 480 gagtaattac ccacttaatggttttgcctt ngacttttgg gttaagaata tttttaaatc 540 ctgnggctnc cttaattggccgnttgncca ngggttccnn aaatgggttc n 591 73 581 DNA Homo sapiensmisc_feature (1)...(581) n = A,T,C or G 73 acgcgggtat ctgtaatttttataattcat caattctgga atgctatata taatatttaa 60 aagacttttt aaatgtgtttaatttcatca tcgtaaaaag ggatcatctc agagagaaca 120 gcagtattct gcgtatttttaaaaatgctc tagagtaaca tttgaagtaa ttcactgtag 180 tgtatgccag tcctagaaataattttttta atttctggtg tctgtttcta atacactaac 240 caagttttca aaatatatttacaaagatgc atctttaccc attattttaa aatgattaag 300 gaggatagtt gcttcaggtaacaagctaat ttttcaaata ttaggccctt acagaactat 360 ttagtcaaaa agtaagatattcctttaaaa tatataaccc aaagctttca gttaaaccat 420 gatatatcac aaatactattaaaatggtaa agagaaaatg caattgcant taatgatgcc 480 caaatngtaa aatatngagattcaaaagct gggnctttat ttaggnggga tnccaatgnn 540 aatgatactg gcctggntttacctttacct tttaaaaaan a 581 74 599 DNA Homo sapiens misc_feature(1)...(599) n = A,T,C or G 74 cgaggtactt tttccgcaca tgccttgtgcctatctgagt attgatgcca tggatgtggc 60 cggagaacag cagctggatg tggaacacaacctgttcaag caacgactag ataaagatgg 120 catccccgtg agctcagagg ctgagcggcatgagcttggg aaagtcgagg tgacggtgtt 180 tgaccctgac tccctggacc ctgatcgctgtgagagctgc tatggtgctg aggcagaaga 240 tatcaagtgc tgtaacacct gtgaagatgtgcgggaggca tatcgcccgt anaagctggg 300 ccttcaagaa cccagatact attgagcagtgccggcgaag agggcttcag ccagaagatg 360 caggaaccag aagaatgaag ctgccangtgtatggctttc ttggaaagtc aaataaggtg 420 gcccgaaact ttcactttgc ccttggggaaganctttcca gcantcccat gtcacntcat 480 tgacttggca aactttggnc ttgacaaccntnaccatgac ccactacatc ancacctgtc 540 atttngggga ggactttcna gccttgggaaacccctngac cccccaatgg taattggcc 599 75 594 DNA Homo sapiens misc_feature(1)...(594) n = A,T,C or G 75 acatcaaatt ataaatgcaa aacaggttcagatttcatct tttgtgattt cttttaaata 60 ctattcattt ttatttaaat gcacagtatttcccctatat tttagtcctt ccattcctag 120 agacaaacca gttatttggt ggtgggaagtagctgaagca aagaaggaaa agtaatacct 180 ttaacctcac tagcttcaag agtagacattcttactagct caatttaaat aattgatttt 240 aaataggaag aaaagaggat atatttaagatacatagaaa ttatgatgtg aagtattcat 300 gagaatctgt agattccatc aaaataagtaggaactcatc taaaattgtt ggatttaaag 360 aggcactttt ggttatgatt caaatatggggaatttgaga aatattcatt ttgnccactg 420 gatggtcact attttactaa aanggnagctttttatgggg ggactgngac tgaggtctta 480 aagactgaaa gaagttgggg ggttcattttcngtaccacc ttcnnggacc atttggacct 540 ttggccggga acacccctaa ggngnaattncngnccctgg gggccgtcta atgg 594 76 585 DNA Homo sapiens misc_feature(1)...(585) n = A,T,C or G 76 acgcgggggg cggagtagca agtggccatggggagcctca gcggtctgcg cctggtagca 60 ggaagctgtt ttaggttatg tgaaagagatgtttcctcat ctctaaggct taccagaagc 120 tctgatttga agagaataaa tggattttgcacaaaaccac aggaaagtcc cggagctcca 180 tcccgcactt acaacagagt gcctttacacaaacctacgg attggcagaa aaagatcctc 240 atatggtcag gtcgcttcaa aaaggaagatgaaatcccag agactgtctc gttggagatg 300 cttgatgctg caaagaacaa gatgcgagtgaagatcagct atctaatgat tgccctgacg 360 gtggtaggat gcatcttcat ggttattgagggcaagaagg ctgcccaaga cacgagactt 420 ttaccaagct tgaacttana aaagaaagctcgtcttgaaa gangaagcnc tntgaaggcc 480 aaaacagagt acanaagttt ccnngttggcttggattttg aaaattcnng aattntntat 540 aacgggcttn tttaaaaagg atnggnttangnacctttnt taaat 585 77 591 DNA Homo sapiens misc_feature (1)...(591) n= A,T,C or G 77 ggtacgcggg agtcatattt atgaaaaaag gtttgtgttt tactcttgctagtgagaaag 60 tgggacaaaa tatacttttg aaataaaatg ctatatggca cctaattattttttctttta 120 aaatgcctta agttgcagtc tcattttgat aatcatttgc ttccagtgtttaaaaattaa 180 aaaaagaatg gggagaaggt tatgagaaga gcattattaa gtttccaaatttaatttgaa 240 ttccaaattc acctagcaat aaaatctaat ttttaaaaag tatataaatataaaatgtat 300 aaatgatgga tagatttttg tattgatttg caaaatgcag attatatttgataggctata 360 gtatgtagat attcctttta ggaatattac agctgtaaat tatatgagacttgccagtca 420 aatgctattt ggtttaaaaa aattattgca atctcaagtt aatggaatatttttaaatcc 480 cacattcaga gttaaaacct ngttttcaat gggtttttan tgtggcacttgnttatagat 540 taatttttaa taacctgttn ggaancnggg ccttttaact ggtccttggg g591 78 252 DNA Homo sapiens 78 actgagaagt attttcagtg attcgacccagaccagattt caacacatgg ttcccataca 60 ggaaggactg ctctgcacca ggctttatccaaactttata cttggcataa ggtgcaaggt 120 aatccagagc tgtgacgtgc aaccgaaacttgtgggtttt agtgaatttt ccaaagcagg 180 tccccagcga caccagcttg tccccggaaatattggcggc cagcttcata atcttctcac 240 tcacatagta cc 252 79 571 DNA Homosapiens misc_feature (1)...(571) n = A,T,C or G 79 gctcgggcaa gcactttaaccttttaagcc caaccagatg agttgcctgc agttttggag 60 gccttcagag catttcactagacctctgtc tgtgtcggtc cagtgtcttt agccaagctt 120 tgattaaaga tgacttccttgtttgctcaa gaaattcgcc tttctaaaag acatgaagaa 180 atagtatcac aaagattaatgttacttcaa caaatggaga ataaattggg tgatcaacac 240 acagaaaagg catctcaactccaaactgtt gagactgctt ttaaaaggaa ccttagtctt 300 ttaaaggata tagaagcagcagaaaagtca ctacagacca ggattcaccc acttccacgg 360 cctgaggtgg tttctcttgaactcgttact gggcatcagt agaagaatat attcccaaat 420 ngggacaagt tcttttaggaagacccctta tccttttgct ggtgaaaatc aaaatgaagc 480 nnaaaatccc ttcaaaatgaggccaacgan taactttttt aaatggcttt tcaaaaagcc 540 ntgttaatta ancttnantgtaaaggnttt t 571 80 595 DNA Homo sapiens misc_feature (1)...(595) n =A,T,C or G 80 acctcttcct gttcgaatgg gttatccagt aaaaaagggc gtgcccatggcaaaggaggg 60 aaatctagaa cttttaaaga ttcccaattt tctgcatttg actcctgtagcaattaaaaa 120 gcactgtgaa gcccttaaag atttttgcac tgagtggcca gccgcactggacagtgacga 180 gaaatgtgag aagcattttc caattgaaat tgacagcact gattatgtttcatcaggacc 240 atctgttcgg aaccccagag cacgagtagt agtctcaaga gtaaagctttccagtttgaa 300 tttagatgat cacgcaaaga agaaattaat taaacttgta ggagagcgatactgcaagac 360 cacagatgtg cttaccatca aaacagatag gtgcccttta aggaggcagaattaccatta 420 tgccagtgta tctactaaca gtgttatatc atgagtcttg gaatactgaagaatgggaaa 480 aaagttagac tgaagccgac ttggagaatn tatatgggaa aatactatcagaaagaaata 540 tctggnaacc cttttccgat gaaagtgctg anaaaatntg gaattaataagaagn 595 81 601 DNA Homo sapiens misc_feature (1)...(601) n = A,T,C orG 81 acgcggggga aaacaagatg gaggattcgg cctcggcctc gctgtcttct gcagccgcta60 ctggaacctc cacctcgact ccagcggccc cgacagcacg gaagcagctg gataaagaac 120aggttagaaa ggcagtggac gctctcttga cgcattgcaa gtccaggaaa aacaattatg 180ggttgctttt gaatgagaat gaaagtttat ttttaatggt ggtattatgg aaaattccaa 240gtaaagaact gagggtcaga ttgaccttgc ctcatagtat tcgatcagat tcagaagata 300tctgtttatt tacgaaggat gaacccaatt caactcctga aaagacagaa cagttttata 360gaaagctttt aaacaagcat ggaattaaaa ccgtttctca gattatctnc cttcaaactc 420taaagaanga atataaatcc tatgaagccc aacttccgnc ttctgagcag ttttgaattc 480ttncttactg atgccagaat tangcngntc ttacccttac tcattgggag acatttctat 540caaagaaaga aagttcagta tctgtaaacc ntttgtccaa gaatttttca ggagagatca 600 a601 82 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 82cgaggtactt tgaatatgga gtagtttaca gctatttttt tttcttactg gtaatcttaa 60ctaatatgat tcccttatta gagagcctct cactccccca cccccaaaaa tgtctactat 120tcatgacagt aaccaattat tctggacaaa ttgcttcttt ttaatttgag ctatctgcca 180tggactttct aaaatggaaa cacagcctga gtgtatctta gggagagttt gattgaaaaa 240atccaaatca ctatccatat agatcatgga tataaagaga tacctgattt ttattaaaaa 300gatacttttt caaatttaag agttaatctt ggaaatttgg aacaagtaaa ggggcaagta 360aaccttttga tgaaatataa aaggactcat tgcatgaagt gactatcaaa ttctgngatg 420tgnggcttct taaaaatatt ctcagggctt tgggggcctg ccanatggta cctgcccggc 480ggccgtcaaa agggcgaatt ccncacactg ggggccgtac tagggggtcc caacttggac 540ccaacctggn gnaaataang gcataantgg tccnggggga aatggtnncc gttccattnc 600cccann 606 83 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C orG 83 gcgtggtcgg gccgaggtac acgttcgtca tggcggctgg ccctggacct gggtaggggg60 tccgggttca gtggtaatag cggcggagat gggggagcct ccgcttggct tctttcacac 120gggttgcttc ggaggaatcc gccgtgcaaa tctgtccgcc cccttggcca ctgatccccc 180gaagagcttc tgtcgccgct ctaggaatac agacattgaa gtttgggaca agatatttat 240ctaacttctg tgtcaaaatt agcgacctgc tatggcaatg aagaaagaaa ctgaatttgt 300cattttcacc tgaagaaaaa tgatagacaa aaatcaaacc tgtggtgtag gacaggattc 360tgtgccctat atgatttgct gattcacata ctcgaagaat ggtttggtgt ggaacanttg 420gaggactatt tgaattttgc aaactatctc ttgngggttt tacaccacta atacttttaa 480tacttcctta ctttactatc tttcttctct accttactaa taatttctta cacattatta 540agaagaaaga tgttttgaaa gaagcctact ntcataatta tnggatggtn caagggaaac 600anggcactnt ntg 613 84 605 DNA Homo sapiens misc_feature (1)...(605) n =A,T,C or G 84 ggtactatct gctgctggca aatggggttg ctctgggtga cagggatctgctgacccaat 60 gctatggttt gttccagtca atgagttgag aaggctaaag ccttggttcctatcattctt 120 catcactaca ttggaccaca cattggcatt cagggcttgg acaattcgctttactcctgt 180 agattctggg aagtcatcat cctcctcagg caactcctct ggactaagttctaccaattc 240 aaagccatgt ttgaggcacc attcttgagc tttttgtcgg tttataccatcttcagacac 300 tctatcgcag accaagatca tcacctcagg taaccatgct tttgccagtggaagccatga 360 ggagacacta tcaaggcccg atttttgtgt gctgtcaaag taaaccacaaatgcttggac 420 agattctgca atctctgcag taaccagaaa tttgttgggc accccacatagattgagtct 480 gctgaaaagt atttattatc aatggncccn ggataaaact acacattatttggaagtact 540 ttcncaataa gaacttntgg tccaaggtat ttttggaccn aanggnctcttgaaaaaacg 600 gagga 605 85 603 DNA Homo sapiens misc_feature(1)...(603) n = A,T,C or G 85 acagggaatg aagactcgaa gaggagatgtcactttcctg gaagatgttt taaatgagat 60 tcaattaagg atgctacaga acatggcttcaattaagaca actaaagaac tcaagaaccc 120 acaagagact gcagagaggg tcgggctcgcagcactcatt attcaggact tcaaaggttt 180 actcttatct gactacaagt tcagctgggatcgtgttttc cagagtcgcg gggacacagg 240 tagagtaaac tgcanagctg cctgtctgtgacttccaagg ctaggtcata aaaggagata 300 aagcttcttc tggctgggtg ggctgcttgctcttgaacct tcagtctatg cacgcaacat 360 gcctttccag ccttctgtgg ttgtagagtgnatagaaagc aattggatca ctatngacag 420 cggggtaaaa cttgaggaag caacctccgccaggnggtac atggagganc cctgaannaa 480 aggaanaaaa gggcacangg gcttaatcctgtcttggaat gcttncctnt gcaatggnnc 540 atttcaatgg ccnagccaat tatgccatccctgcnttaan accatgggcc ttcnttgnca 600 ttn 603 86 583 DNA Homo sapiensmisc_feature (1)...(583) n = A,T,C or G 86 actgtaggta tttattaataatagcaatga agatgaaaga gtgatgtatc agagaggtgg 60 agataaaatc agtaaaacttagacactaaa tgatagggga aggtggagga gaggaatgag 120 cctagaaaac ttagaatataatggttctaa aattaaccaa agtaagggac acaggcatta 180 gagtaggttt tgcagagaatgaatgtttta agacacacac aggtgtctct gggacaacca 240 agaaaagtgc aacaggcagatggattgagg agtctggcta aagataagga tttaggaact 300 gctgaattaa aattacccaagcgtgagaag tggtgttgtg attaagagag aaaaaaaaaa 360 tggaggtctg aggaatacctttaanggatt aatgaanang cccaaaggtg ggggggtggt 420 caggagtgac ccaaatgtagaagtcaggga ataaacttta aagtnggggt gtcaaaatgc 480 naatccgaaa aaaagtnagtnccttggccg gaccccctag gcgaatccac ccctggngcc 540 gtctanggat ccacttgnccaacttgggaa nntggctnct ttt 583 87 332 DNA Homo sapiens 87 acgcgggggcattgctagaa gccggcagga gtgactctcg gcatggagga cccatctcct 60 agcacacgtgcccactgaag tggcaccaac agaagtttgg cttgaactaa aggacatttt 120 attttttttactttagcaca taatttgtat atttgaaaat aatatatatt attttaccta 180 ttagattctgatttgatata caaaggacta agatattttc ttcttgaaga gacttttcga 240 ttagtcctcatatatttatc tactaaaata gagtgtttac catgaacagt gtgttgcttc 300 agactattacaaagacaact ggggcaggta cc 332 88 592 DNA Homo sapiens misc_feature(1)...(592) n = A,T,C or G 88 cgaggtacgc ggggacaacc agctgactcccgtagaggaa gacactgtgg aggccagttc 60 tggagctatt gcagcctcgg ttgcccggcccgggacccga acccgaaaaa gttatcgtca 120 gaatgtcggg caaagaccga attgaaatctttccctcgcg aatggcacag accatcatga 180 aggctcgttt aaagggagca cagacaggtcgaaacctcct gaagaaaaaa tctgatgcct 240 taactcttcg atttcgacag atcctaaagaagatnataga gactaaaatg ttgatgggcc 300 aagtgatgag agaagctgcc ttttcactagctgaagccaa gttcacagca ggtgacttca 360 gcactacagg tattccaaat gtcaataaagccccagtgaa gattcnagcn aagaaagata 420 tgtacnagtg gtactttgnc ngtatttgaacattccntga aggactgcng gttttnactg 480 cttgggttaa cccaagtggg gacnnnttgcttaaattaaa gaggaatttt gcccaancnt 540 gggacttctg gnggaattac ttttttggaaactttttggn accttggagn aa 592 89 630 DNA Homo sapiens misc_feature(1)...(630) n = A,T,C or G 89 acgcgggggt ctttgggccg gcgcgaaccatggccggcat ggtggacttc caggatgagg 60 agcaggtcaa gtcctttttg gagaacatggaggtggagtg caactaccac tgctaccacg 120 agaaggaccc ggacggttgc tatcggctggtggactattt ggaagggatc cggaagaatt 180 ttgatgaggc tgccaaggtg ttgaagtttaactgtgaaga gaaccagcac agtgatagct 240 gctacaaact gggggcctac tatgtgactggaaaaggtgg tctgacccaa gacctgaaag 300 ctgccccagg tgctttttga tggcgtgtgagaaacctgga aagaaatcaa tagcancatg 360 tcacaacgtt ggccttctgg cacatgatggacagggtaat gaagatggcn acctgacttt 420 ggaaaaggca aggactacta ccaaaggcctgngatggngg ntatctttca gtgcttnaaa 480 cctaatgcat ttttcttcag ggggcccaagctttccaagg acatggcctt gcctgtnaat 540 cttcattaaa gccttgacct ggtcatatttggccttgcca tgcaatccat ttacttggcc 600 ggacacctan gggaatcacc actggggcgt630 90 653 DNA Homo sapiens misc_feature (1)...(653) n = A,T,C or G 90ggtaccactt cactccagcc tggcgacaga gtggaactcc gtctcaaaaa ataaaataaa 60ataaaataaa gcaaaaatat aaaatgttaa aaaaaaaaca aaaaaaggga aaaaggaagc 120tgattgcctt ggtgagtcaa cactgggtat tttctgacca ctatttgaaa caaaaaagga 180aaccactgat attctatgca aagatctgtt cctggaaggc actctgcgga gacaccagga 240gaacttttat caatccttca ttgatttgaa gtaaaagtgc taaagcaatg gttggtgggt 300ggcaacccat tagcagatca caaaatcact gtagtgggta actaaacaag aggaaacaca 360agacggcatc ctgtgtaact ggggttaagc attactctct gaaactcatg gcatcagttt 420cctcttaggc tcttcccaca aagtataatc atgttcattt cagtttacaa tcccttgcag 480tcccatcgat ttgtgagaat atcccaagtc atncacagng gagnctggaa atggtcntan 540ttgtcctgcc cggcngccgt tcnaanggcg aattcaacac actggcngcc gttctaatgg 600atccaactcg naccaacctg gnggaacatg gctactggtt ctggngnaaa tgn 653 91 657DNA Homo sapiens misc_feature (1)...(657) n = A,T,C or G 91 actttttttttttttttttt tttttttttg ggagaaaagc ctnactccgt tgcccacgtt 60 ggagtgcagtggcgtggnca tagcttattg catgcagcct naacctccca ggctnaagca 120 atnctccnacctnnncgtgc tgnnttnntg gaactacnca tncacncnat tatgcccanc 180 tngtngttgtnaatttaaag tganaccatg cncncaggnn gnatggcntt nnntancnan 240 catgcatgctcagctgtgta gtgcacgcac aggataaatg gaagggggat ttgatcaggg 300 tttttgtcacatnagcattn naaatccgna ngactgccnt gtgtctgcct ttgnaagggc 360 ctgggagtattctgtgtagc ctttgnaaat aagggnaaaa tgngcncctg ccaaagaagt 420 cnttgctactntgggtgngt caaaatntcc ctgtaacttg tcaatggnca caagcttggn 480 ggngtntttgggntcttggn tgtcnttttn acgtctattg nccatgtggt tcctatatga 540 cacantcctcntnataatcc ntganaattg ctaanntgcn cttttttttt ttttnanatt 600 nattttgctnttaaantagc ttaanncttt ntttatcctn gggcanccna anncaat 657 92 653 DNA Homosapiens misc_feature (1)...(653) n = A,T,C or G 92 accataaaac cattaaaagcaataaataac tagagtcatg tgagatgttt caaagactgc 60 tggaggtttc tgtaaaccagggtaatcaga aatattaccc ttgtagatag ccctctcata 120 ccagtaaata caaagagttaaaattccaat gccacagtgt aacagttaac aatctatttt 180 gtaattttaa atattactacattaattcac cctgagaata cagaggaaac atttaataca 240 agacattctg atatgnttttttttcccatt gnatttgctt tcttctggnt ttcatcagcc 300 ctttaagggc acagatattttaatttaaag ggtgatttgg atatgctttt ttggtaactg 360 agatttatgc cacagtcagatactggtgat agaaaagccc aaaaaggntt gnagaaaaga 420 ggcaagcagc aatccccaggcagaaaagac ngaaagtctt gaaaaagaag aggagtaaaa 480 atttttttaa gctgntcaatgccctgtatt tgggnacaag tacctttatt ttcctttagc 540 tganggnant cagagtaaccgaattggnag nnnactattt tcnctggnaa ggaaaataga 600 atttggnaat cccnggaanggtnctngaaa tnnagcccca tccatttgnn gng 653 93 640 DNA Homo sapiensmisc_feature (1)...(640) n = A,T,C or G 93 acagagaaac cacaggttgccctttccaca gctggataga cttatccaaa acggcaggat 60 ggttctgtat taatctttttggaaagcatg tctgtattaa gattgcaaaa catacagata 120 gctaccacaa attaggtcaaacgactgatc aagttgtaac atctgtgagg tcaaattcca 180 aagtgcctag atacacatttatacaacaga ccataagagc tgaattcttt acaaatgtct 240 ttatgggcat gtaaaattgactctgcattt ctgcatgtgt gcattccata agagagacca 300 gtctgcactg agtcatatatactccaactt gaaaaagtaa gtgnaacaac tggntaatca 360 tgcaagtctg gttgnaatataacaatgact ggnaaaacat gaattcttcg cacagtagta 420 ataggngcac tnatttaaaaccctnccgaa aaacctgnat ttggtgcaan atctganttt 480 aagnggtagt aacttgacntttaaaaatag tttgaacnat ttanaaaggn aagccaactt 540 ttacttaaaa gaatcccaagtggnaaaanc tggntttcaa tggaatgaac tnggtgngac 600 ctnccctaat nngaccttgagcctatnagc taatntangg 640 94 658 DNA Homo sapiens misc_feature(1)...(658) n = A,T,C or G 94 acgcgggcca agcttttttt ttaatttggtgttttctccc atcctttccc tttaaccctc 60 agtatcaagc acaaaaattg atggactgataaaagaacta tcttagaact cagaagaaga 120 aagaatcaaa ttcataggat aagtcaataccttaatggtg gtagagcctt tacctgtagc 180 ttgaaagggg aaagattgga ggtaagagagaaaatgaaag aacacctctg ggtccttctg 240 tccagttttc aagcactagt cttactcagctatccattat agttttgccc ttaagaaagt 300 catgattaac ttatgaaaaa attatttggggacaggaatg tgataccttc cttggntttt 360 ttttgcaanc ctcaaatcct aacttcctgccccacaatgg tgagcaggtt cccctgatac 420 ttcttttctt taatgattta actatnaacttgnataaata acttataggg gatagggaaa 480 attcctgaat tccagaatgc catctgntaaaaaagaatnn aaatgggaag tnggactnaa 540 aaggagccaa cagcatgctg cggtggnngncacttctttg cnctatccca ggaaggaagg 600 tccccatttg gaaaggggtt cttnctcactggnaccggtt tgacntnatt ggnacncc 658 95 392 DNA Homo sapiens misc_feature(1)...(392) n = A,T,C or G 95 actcagactt gatcgattaa tgaagtggttattttggcct ttgcttgata ttatcaactc 60 actggtaaca acagtattca tgctcatcgtatctgtgttg gcactgatac cagaaaccac 120 aacattgaca gttggtggag gggtgtttgcacttgtgaca gcagtatgct gtcttgccga 180 cggggccctt atttaccgga agcttctgttcaatcccagc ggtccttacc agaaaaagcc 240 tgtgcatgaa aaaaaaagaa gttttgtaattttatattac ttnttaagtt tgatactaag 300 tattaaacat atttctgnat tcttccaaaaaaaanaaant aatnaantta naanctttta 360 aanatanaaa taaaataata angaccattg ag392 96 655 DNA Homo sapiens misc_feature (1)...(655) n = A,T,C or G 96ggtacaggtt tttatgtgaa catacatttt cattttctgg gataaatgct caaaagggca 60actgttgggt tgtatggtaa acacatatat ttttgtaaga aactacccta ctctttttcc 120agagtggctc tactttttac atacagccac tcatacaatt cagacagcaa tgtatgattg 180atccagtttc ttcacatcct caccagcatt tggtattact actatttttt atcttaacca 240ttcacataga tgtgtgtaat gataccacat gtggttttaa tttgcatttc caatggctaa 300tgatgttgag tatctttttg tgtgctaatt tgccatctat gtatcctctt cggtgaaatg 360tcttcatgtc ttttgnctat tttctattta agncatttgg tctttttact attgagtttg 420agagggtttt tatatatcct agataaaaat cctctggtan anatgtgggt gcctggaatt 480ttaacataac ttctacccan ggaaaataag taaaatttcc acccttgctg gcnagcctta 540cttaatnccg gccttaangg tccttctaga gaattaagaa gatttgaggt ttaaatanaa 600tcagggcntt aaaaagtaat cctaaaatcn ggtttaagca agccatatcc tgggg 655 97 224DNA Homo sapiens 97 acaagtttaa ggtaggacgc agcattttat agtgttacgtccttcctccc cacattcctg 60 tgaggcggaa caagaacaat tacttgaccc tggaggaagacgacgccttg tggtcaggga 120 gagaacagca gttcatgctg gctgcctcgt ctttccaggcctgctgctgc ccaggcttct 180 actgaccttg ttaggtctga ttctagaaaa tgaaggcaggtacc 224 98 582 DNA Homo sapiens misc_feature (1)...(582) n = A,T,C or G98 ggtaccacca tgcctggttt attgttttat ttttttggca gagatgggtc tcactgtgtt 60gcccaggctg atctcaaact cctggcctca agcgatcctc ccatctcagc ctcccaaagt 120gctgggatta cagacctgag ccaccacacc tgggcaacag agtgaaacct gtccctgttt 180tcctgctctt actctcacct ctgaggcctc ctctgcctgg aagagattac agggaaattc 240caggcagccc ttgtcaattg tttttatgaa ttctttacct gttcctttta aagacaagga 300aactgaggcc caaagttcta agttgtttgg caaatggagt ctcctaccct cagctcctgc 360aaggacctgg gggaccccca ggtccagcag ccacatgatt ctgcacagac agggacctag 420agcacatctg gatttaagcc caccctggca actggctgct agagactncc aagatgccga 480taataggatc tgccnttaaa aaatctggat tctggcctgc ntaantgcta cttcatttgg 540ctacaaagnt ttaaggngga accnttaaaa ccttccccaa aa 582 99 619 DNA Homosapiens misc_feature (1)...(619) n = A,T,C or G 99 ggtacagtgg tccttttcagagttggactt ctagactcac ctgttctcac tccctgtttt 60 aattcaaccc agccatgcaatgccaaataa tagaattgct ccctaccagc tgaacaggga 120 ggagtctgtg cagtttctgacacttgttgt tgaacatggc taaatacaat gggtatcgct 180 gagactaagt tgtagaaattaacaaatgtg ctgcttggtt aaaatggcta cactcatctg 240 actcattctt tattctattttagttggttt gtatcttgcc taaggtgcgt agtccaactc 300 ttggtattac cctcctaatagtcatactag tagtcatact ccctggtgta gtgtattctc 360 taaaagcttt aaatgtctgcatgcagccag ccatcaaata gtgaatggtc tctctttggc 420 tggaattaca aaactcagagaaaatgtgcc catcangaga acatcataac ccatggaagg 480 atnaaagccc caaatggnggnaactgataa tagccctaat ggctttaaga atttgggcac 540 actnttacct aggngaacccatttganccn anggggctta aaggcttntt acttcaactg 600 aaagttnagg gaaaaaaan 619100 614 DNA Homo sapiens misc_feature (1)...(614) n = A,T,C or G 100acgcggggga agcaaaggag agggaagctg gaagcacctt tggcccggga cagaaatctg 60gagagcttgg ctacctccat cctcctcagg ccggagcagg cttcctgaga gagtccaggt 120cgtaggagtt ttacgactta gaaaagcggg ctgcagattc cttcctgggt gtttggttca 180agccctggct ccagcctcac tctcagtctt cccgggagtt cgtgggattt ggaccttaga 240ttattagtat tattttgagg gcctcctgtg tgtaagcact ggttgtgcgc agatggctgt 300gcagagggcc atgaggtaga ggctggggaa atgagggctt ggaggtgctt gaggtatggt 360ctttacctac gtgaaatgtt ggaggttgag atgaaaactc ttgctttgaa atcttcatgg 420aggactacat catttcaatc ctgaatctgg ctcaattcta ttaatcactt aatacctgga 480ttaaaaaacg nttaantggg ccaggcncaa tgggtcacgc ctgnaatccc agccntttgg 540gaggccaagg cangccggat acnttagggc ngnanttnaa accancttgg caaattggga 600aacccgcntt tntn 614 101 625 DNA Homo sapiens misc_feature (1)...(625) n= A,T,C or G 101 ggtactttgc ctacggcagc aacctgctga cagagaggat ccacctccgaaacccctcgg 60 cggcgttctt ctgtgtggcc cgcctgcagg caagaagggg ttaaaagtggaatgtatgtt 120 gtaatagaag ttaaagttgc gactcaagaa ggaaaagaaa taacctgtcgaagttatctg 180 atgacaaatt acgaaagtgc tcccnnatcc ccacagtata aaaagattatttgcatgggt 240 gcaaaagaaa atggtttgcc gntggagtat caagagaagt taaaagcaatagaaccaaat 300 gactatacag gaaaggtctc agaagaaatt gaaagacatc atcaaaaaggggnaaacaca 360 aactctttag aaccatancn gaatatatct taagggtatt cctatgtgcctaatataata 420 tatttttaac acttgagaac cagggatttt gggggattct ccaacgtttgttcaatttta 480 agaantggtt tgaaggagtt ttttacttgg gtnattcntg gttttaggattttnnanngn 540 aanntggntt nggngtttgn nnttttaann gggntntttt ngggtcttnaaatttttcca 600 anaaanngtn gnttccttcc cggnn 625 102 605 DNA Homo sapiensmisc_feature (1)...(605) n = A,T,C or G 102 ggtacaagaa agaaaaaatataaaaacaag tctgctgagt gtcgggagtt ggtgagggat 60 atcctaccat attgtgacggagtccaaata gaaaacatgc agcaacagtt ctcctgcttt 120 atcagctccc tggaaaataaaccagtaacc ctggtagtgc agtaaccatt tggttaacag 180 gacaaacttc ctgatggacacagatagtaa ttcactgcat ttcccttctc taacttctct 240 cttcacacca attccttttctttcctttaa gatgggtttc atcctgttga caaaagattt 300 ggttttattt gtaaagtaaagcagataata tcctgattga agtattcaat gatttaattg 360 aggatgcttg gggatcaaactttgtaaaaa ggtcaattaa gctagttagc agagactatc 420 agtggcttgc agaaaaaaaantcngatata tggtttggta aaangcccaa aggataaccg 480 ngaaaaatcc tanggataccgggacctaat taatcaaagc canaggggga ccttggttaa 540 anccnttact tnggggangggctnaanggn ggntccaaac naaattggtt cccaacgggc 600 ccggg 605 103 251 DNAHomo sapiens 103 acgcgggatt ttacattcca tcttttctga agattgtcct acaatttggattttgatcat 60 gacaaagaag attaaaattt cattagcatg aatgcaattt gttaaagcagactgatttgt 120 ttctaagata tttttggttt ttttaaaact gataataatg ctgaattatcttaagtgaga 180 tgttaagccc actttgttct tttaatgtaa tggagcttat gggtagaagaccatgtctac 240 taattacaaa a 251 104 293 DNA Homo sapiens misc_feature(1)...(293) n = A,T,C or G 104 ttaatcttgc acaaatggca ttttattaaagaaaatctaa tttacaaagc tttgtaaatt 60 ttaagaaaaa cattcataga tcataaacaaaaatttcaat atgcaatatt caaatttaca 120 agaaaataag cacaaacttt tagacagtgcagttattgct gcactccttt aattccttat 180 ccagagccca aaaaatgtag acaaaccctaaaaatgtagc agaagcattt ccgcacactg 240 gtgtccagaa tctagtttgt gcanaaatgtttccactaga tttatagagt acc 293 105 586 DNA Homo sapiens misc_feature(1)...(586) n = A,T,C or G 105 acatcatttc tgccatgtgg gacattttcttgggaatata caagtaatac tccatgtagc 60 ctgacaggtc ctcaatggtc acatcatccacgaagactcg agcttgctca gaacaggatc 120 ggggagagcc agacagagtt ctggcgtgcagcgactgaga gtagtcctca agtgtggatc 180 ttcgttctgg agccaaggga gggacactctgcgggcctga aaaggaatac acttccatat 240 catgccatct cttacactgg cattccttgcctatgcatgt gcatggcttg ccctggttta 300 gcttggaaac tgattgaaag tcagagagatcactggcttt gagacttgct tgggggactt 360 gggtagccgt cagaggagtc ttccttcttactctctgatg ggagccttgg aacagaaagt 420 tctcaaangc tnaacgactg gccctggggtgaatagcatc gagagaagta naccttcttc 480 ctgnactgaa ctnttaaggg gatgaaattcccagccaatg gtggccttan gnnangcaan 540 ntggcttttg gcttgaatta ctggntggaaaaaacctttg gccntt 586 106 644 DNA Homo sapiens misc_feature (1)...(644)n = A,T,C or G 106 ggtacnttga ttgctcanat ataangaaat ggcccaatgaacgtggntgn gggaggggaa 60 anangaaaca gagctagnca tatgtgaatt gntctgtgnnataaacatgt taaaacanac 120 aaanatggnt atttttcttt ncctccggac agtgcacattatcatntgaa ctacctgggg 180 attctntatc anaactggtc ttgttgaata tttatacttaattgaaataa ttccttanng 240 gaggcntgtt taaaacgtat taacaggana ttgtgtntnanacatttaat gaaanacgaa 300 attccacnag aatganntaa gtcactttcc aagtgggtgtcattttgtta aaccctngtt 360 tacctgtttt gctattntta ccntttcatt tggaangatgntttgagntc gtanttacca 420 gggnaaagac gggttncttc ctngctgnnn cttnagccnntgctaaaaag cnttaatttt 480 ntgcnattng gnncttcctg ctggtaatcn tggaaaaantgggnnaantc cagctttntt 540 tnttggcngc ccaaaaangg attcnnantn gnnannnaacctttggttcc ntaannaana 600 aaangtatnc anaangaacc ttgncatgcc ngccnntntaaang 644 107 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,C orG 107 ggtacagact tgccctttga aatctatacc tctggataca ttagaggcat tttattaaca60 aaggcccttc taaatgtgct atttatttga caataactat cagatttgcc ttaattttgt 120gtttatagca tttatcaaaa cgtatcctca tagactttat gcagattaat atggtcaatt 180gatttggata aaagaaagta atttcagggt ttgtttttaa gccaggacaa gaagtgcaaa 240tgcctctttg aagcaattta ggctaaactg attttgaaat ttcaaaatgt tttattttac 300tttgttttat taagccagga caagaagtgc aaatgccctc ttttgaagca attcaggcta 360ggtaaacccg attttggcca tttcaaaacc gtttaattta ctttggttta atatcagagt 420cttataaaac tgntgncaaa aatttctgaa ggctttngaa aagggttggt agtggaccct 480gcccgggcgg ccgntcnaag gcgaattcag ccactggcgg ncgtactagg gatnccactc 540ggacccanct tggcggaatc atgggcataa ctggttcctg ngtgaaatgg gatccgttac 600aattcccaca acatanng 618 108 620 DNA Homo sapiens misc_feature(1)...(620) n = A,T,C or G 108 ggtaccaaag gagaatttgg agagctggctaaattatttg aagaaagaat tgccaacagt 60 ggtgttcaga gcctcaacaa aaccaaaggataaagggaag ataaccaagc gtgtgaaggc 120 aaagaagaat gctgctccat tcagaagtgaagtctgcttt gggaaagagg gcctttggaa 180 acttcttgga ggttttcagg aaacttgcagcaaagccatt cgggttggag taattggttt 240 cccaaatgtg gggaaaagca gcattatcaatagcttaaaa caagaacaga tgtgtaatgt 300 tggtgtatcc atggggctta caaggagcatgcaagttgtc ccctttggac aaacagatca 360 caatcataga tagccccgac cttcatcgaatctncactta attccttctt tgngccttgn 420 ttttgcnaag ttcanccaag gttttgaagtantaaaancc gatggaagct tgccantgcc 480 atcctttcca agcttgatgc ttgacaggtagtancttgnc cgggccggcc gttcnaaagg 540 gcgaattcaa cacactggcn gccgtactatggatccgagc ttggnccaaa cttgcgtaat 600 catggcatnc tggttcctgg 620 109 317DNA Homo sapiens misc_feature (1)...(317) n = A,T,C or G 109 tttgtatttttagtagaggc agtgtttcac cgtgttagcc aggatggtct cgatctcctg 60 acctcgtgatccacccacct cgacctccca aagtgctggg attacaggcg tgagccacca 120 cgcccggcctcttttttttt tagctgccaa tctttttgaa ggaatattct tacctctact 180 ttgtcaccttctactggctc cttaactaaa atctgccatt tggctctctg gttaacagtc 240 ccttcctgtaaagtctaaaa tcttaattct aaatccacag tttaattcac aagctagtac 300 cttggccgngaccacgc 317 110 603 DNA Homo sapiens misc_feature (1)...(603) n = A,T,Cor G 110 ggtacattca ggatccctcg gccaaggact ggaccagaag aacacttgggaatcttgggt 60 ccacttatca aaggtgaagt tggtgatatc ctgactgtgg tattcaagaataatgccagc 120 cgcccctact ctgtgcatgc tcatggagtg ctagaatcta ctactgtctggccactggct 180 gctgagcctg gtgaggtggt cacttatcag tggaacatcc cagagaggtctggccctggg 240 cccaatgact ctgcttgtgt ttcctggatc tattattctg cagtggatcccatcaaggac 300 atgtatagtg gcctggtggg gcccttggct atctgccaaa agggcatcctggaaccccat 360 ggaagaccga gtgacctgga tcnggaattt gcattggtgg tcctgaattttgatgaaaat 420 aancctggna tttggaagga aatgtgcaac catgggtcca agaatccagccnnattaacc 480 taccggatga acctttnttg gaaaccataa aatgcctgca atcaatgggaactttttcca 540 accttanggg cttaccatga ccttgcccgg ccggccnttt aaanggccaattccaccccc 600 tgg 603 111 595 DNA Homo sapiens misc_feature (1)...(595)n = A,T,C or G 111 acatttaagt tcccatgtta cagaatccca tattgtgactatttcctcaa aactaactgc 60 tagtaaagaa ccatcttcgg agaaacaaca gttagttgcttgatacttgt gataactacc 120 aacaaagtca caggtccagc caacagcttt tttgtatatgtcagagtcat ctgttaatat 180 ccatactttg aagtaaccat ctttgctagc tgtaaccaaggtgggctgtt cagatttttc 240 tgcattacag aaacagagag ctgtaatgca gtcttcgtgtggcatgttaa ttttagtgtt 300 aagaataaac ccttgtgttt tcttattata catccacagtttcatttgca attcaagctc 360 aagtttcctt ttcttgccgc tggtccactg gtgcaagccagttaccaaag cagccaatgc 420 aagccttggt aagtcaattt ggatcaganc ataatcantaatatatcctg ctggataata 480 ctaaattgga tactggntat cactntggag agaataaactgcaggtggcn ggntttcatt 540 caaaccaagc tttagtcttg gacaatcatn aaccagngaaatactcctat ntttn 595 112 523 DNA Homo sapiens misc_feature (1)...(523) n= A,T,C or G 112 acaagagcta ttagagatgc tgccatatgg atgggcaaaa ctgagccaatcccacttagg 60 aatggaaggc ttggacatgg aagggaggat ataaacgagg agttggagaaaaacgcaagc 120 ccagtttttg ctagagtgga aatgaaagtg ggaatgaggg tcttgtttttagtcctctaa 180 ggaccaggaa gcaattttaa aacttccttg gtttttctga aagcagcatattcaaaatgc 240 cagcaaaaac tcctaacaac tgcaaaacca aaagaggatc aaagctcaccaacatccctt 300 cttattgctg aaaggctcta aaattcagga tgccctgttc ccttgtaaaagggaaaataa 360 ttaaagtctg atttatggta atcataccac atcacacttc taaaaaaatatttcaagtgt 420 gtgaccaggg gaccgtttga ccnccatttt attaaccttc actttantgggaaaaataaa 480 accttttcca gggccatttn atnccaggac ttttagtagg ggg 523 113578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 113acagtgtaaa taactaagtt gttaactgtc aagtccagtt atgtattctg taagttgtgt 60tctagtcttt gactaaaatt tatcatctct tataatggga cttaatcttt ctctaaaagc 120atataagagc ttgtcaatag agcaatcaat caaaaagatt ttgtgattca taacattgaa 180gttagtctgg ttaagagttt tggtttagac ttcatttata ttttccttac taatatctaa 240tatttaatga ataatgatca attttttata aagttattaa tatgatcagg gaaacctttg 300ggacttctga caggcatctg gtgaagagac aattcaagcc ttagtgacta tttagaatag 360ccagtgatca ctagctaatt ctcatatcca tgcctttttt gccctggtta cagtcttaaa 420agaggtaaaa cagcaaatat tttttttaag ggaactataa ccctangaat tcctgaaaag 480aatttcaaaa aaaataagac cctgtggcca tggngnccaa acntaagacc tactatggct 540atattggtcc attaaaaata aattactact aatccaaa 578 114 613 DNA Homo sapiensmisc_feature (1)...(613) n = A,T,C or G 114 acggtagtaa gaaacctttgagatctttct gacttttcaa aattagagaa agcaaatggg 60 atggatagat tttttttttcttttcaaggg gggcaggaag gtaatggttt gagtagcctt 120 tgtttaaaaa aaaactaaatatatttaaaa ggccacattt atattttttt cacaagaacc 180 acataataaa ttccacttcttgacctgaat ttggaaatcc gaaattacta atccaggcca 240 ggtgtggtgg ctcatgcctgtaatcccagc actttgagag gccgaggtgg gcagatcact 300 tgaggcctgg agttcaagaccaccttggcg aacacggtga aaccccgtct ctacgaaaaa 360 aaaaanatat aaaaaaagtactggttatta accaaccagc ttagaaaaat aatcatggtn 420 gacacnttan ttcattcttctaaaagcctg ttgatctggg ccttcctgtt gccagcattt 480 cccctttttc aaaaatggggggccttttct ttaattnnac ctcgtggngn aananaattt 540 gaagggcccc aggaagttntttgggcnctt tgaagcgttt tncacncgtn tagattctnt 600 gattaaatcc tcc 613 115190 DNA Homo sapiens 115 ggtacattgc cactgagtaa agagtggcac cagccacggtggtaggtgga agaaacatag 60 atcccaatga ggacacaaag acgagaccca ggcccactcccaggggtgca cccatgttca 120 gaaacttttc actgggcgca cacatggcca cagtggagaggcctcccaca atgccagctg 180 tgtacttttt 190 116 610 DNA Homo sapiensmisc_feature (1)...(610) n = A,T,C or G 116 ggtactcttg gtttatcaatgggacgttcc agcaatccac acaagagctc tttatcccca 60 acatcactgt gaataatagcggatcctata tgtgccaagc ccataactca gccactggcc 120 tcaataggac cacagtcacgatgatcacag tctctggaag tgctcctgtc ctctcagctg 180 tggccaccgt cggcatcacgattggagtgc tggccagggt ggctctgata tagcagccct 240 ggtgtatttt cgatatttcaggaagactgg cagattggac cagaccctga attcttctag 300 ctcctncaat cccattttatcccatggaac cactaaaaac aaggtctgct ctgctcctga 360 gccctatatg ctggagatggacaactcaat gaaaatttaa agggaaaacc cttangcctg 420 aaggtgtgtg ccacttcagagactttacct taacttgaga cngntcaaac ttgcaaacca 480 tggngnggaa atttgccgaactttacactt tgggcaggtt ttttcccaga agtcanaaca 540 agaactcctn ntcttganaagggttttanc ccctttnaat ggccttgctt atgctgcctt 600 tttcgtttgg 610 117 608DNA Homo sapiens misc_feature (1)...(608) n = A,T,C or G 117 ggtacgcggggggtattatt tgtgccaacc aatgatgctt ttaagggaat gactagtgaa 60 gaaaaagaaattctgatacg ggacaaaaat gctcttcaaa acatcattct ttatcacctg 120 acaccaggagttttcattgg aaaaggattt gaacctggtg ttactaacat tttaaagacc 180 acacaaggaagcaaaatctt tctgaaagaa gtaaatgata cacttctggt gaatgaattg 240 aaatcaaaagaatctgacat catgacaaca aatggtgtaa ttcatgttgt agataaactc 300 ctctatccagcagacacacc tgttggaaat gatcaactgc tggaaatact taataaatta 360 atcaaatcatccaaattaag tttgttcgtg gtagcacctt caaagaaaat ccccgtgact 420 gctatagacccacactaacc aaaggtcaaa attgaaaggt gacctgaatt cagactggat 480 taaagaaaggtgaaaccatt actgaaagtg gatncatggg gaagccattt tttaaaaaat 540 nccccaaancattgatggga attccttnng gaaatacttg aaaggaaccn nnnnagacca 600 atcnttcc 608118 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 118actccactta gcaaatgccc tgccagcaaa gtcacagatg acttttttac ccaatcttag 60gtaaatctgg attatctgcc caaccgtgca agtcaataag ccacccttga aaactgtgtc 120aagatttgag gaaacaggtc ttaagaacct atccaacaca tgattccata accaatacat 180cttangttgt tttaggcaaa taggtgtatc tcttgaatca ctgatggatt caatatcaag 240atctataatt ttcacgttta aaatttactc tgccgaggac attttattgg taaagcataa 300accagttagt ttgacagaca cnaaaaagaa aacnaaatgt tcacagtcct atcttcgtag 360ggattcttgg ctataaaaat tggcttcagg ttcaaggtct tagaccactc ttctaaggct 420nctactggat atantantta ccacttgggg nccaaactta aaacctcntg gactttttcc 480ccttanggac nangaaaaac caaggggttg tggtttgaac tccntacact tggngnnaaa 540ncttttcttg gnngnatnta aanattaagg ggcttttn 578 119 584 DNA Homo sapiensmisc_feature (1)...(584) n = A,T,C or G 119 actgtcttag aatattatttatttttttgt atttgtaaat ctgtggacaa aagagggttt 60 cctcactcct tttactcactgggctcatga cagtgaagga gatgctccat ctgcttctcc 120 ccctttctct tgctgtagtccaatgtgcta tgagcatcag cttactttgc cacttagagc 180 aagcaaaacc cagtgcaagagtctcgttca gctctaaata ggtttgcttt cttttagtta 240 cagtgcccat tttgaaattgcctatacagt cttagtgacc atttaaaccg gacgaactan 300 gcgtttaatt ttcacttcttcatgttnaat tngcagttca anatttatag naagatggnt 360 atttcgaaaa nacaaaaaantggnttttta anaaaanaag tncnttggtc ggcgaancan 420 gcntaagggg cgaatttccagcncaactgg gcnggcccgt nncntagngg atccccaacc 480 tttggtaccc angcttnggcnntaancaat tggnccanag nttgtttccc tggggtgaaa 540 antngtnatc ccgttcccaattcccnnaca ncnnaccnng cccg 584 120 587 DNA Homo sapiens misc_feature(1)...(587) n = A,T,C or G 120 acgcgggggc cgtagcagcc gccgcccatccctctttgtg tgctttggaa agccgcggag 60 ctggtggtgg ctacagttgg tgttgggggcttaggcgagg gacgttaccg ggaagttgca 120 ggcgggagga ctcttcccca tccagtcacctgacaggtca caaacatgtc agacaaaagt 180 gaattaaagg ctgagttgga acgtaagaagcagcgactgg cccaaatcag agaggaaaag 240 aagagaaaag aagangaagg gaaaaaaaaagaaacagacc anaataagga agctgttgct 300 cctgtgcaag aagaatcaga tctttgaaaaaaaaaggaga gaagctnaaa gcatttgctt 360 caaagcatgg ggctaacttc agaaatccccccattggncc ttcctnctaa tncttncatn 420 ccttcaaaat ctgtggagcc ctttccaaggtgaaacttgn aannccaaga antntggaaa 480 atggcnccct tggggaatct agaccnagggncctttttna accttggaat ngnttaaaaa 540 tcacnccaag nttgactttt ccttccttcganaaaattgg gtcccnn 587 121 570 DNA Homo sapiens misc_feature (1)...(570)n = A,T,C or G 121 ggtactcttg gtttatcaat gggacgttcc agcaatccacacaagagctc tttatcccca 60 acatcactgt gaataatagc ggatcctata tgtgccaagcccataactca gccactggcc 120 tcaataggac cacagtcacg atgatcacag tctctggaagtgctcctgtc ctctcagctg 180 tggccaccgt cggcatcacg attggagtgc tggccagggtggctctgata tagcagccct 240 ggtgtatttt cgatatttca ggaagactgg cagattggaccagaccctga attcttctag 300 ctcctncaat cccattttat cccatggaac cactaanaacaaggtctgct ctgcttctga 360 agncctatat gctggagatg gacaacttaa tgaaanatttaaanggggaa aacccttaag 420 ccttgaggtg tgtgnccact tcanaggact ttaaccttaactttgagacc aggtcaacct 480 ggnaanccct tggtggagaa attggccgaa cttcccnactttggccaggn ttttcccang 540 antgtcaaan caagacttcc ttatcatgnn 570 122 551DNA Homo sapiens misc_feature (1)...(551) n = A,T,C or G 122 actatctctattcaggatta tgaagttttt cgatgcgaag attcactgga tgaaagaaag 60 ataaaaggggtcattgagct caggaagagc ttactgtctg ccttgagaac ttatgaacca 120 tatggatccctggttcaaca aatacgaatt ctgctgctgg gtccaattgg agctgggaag 180 tccagctttttcaactcagt gaggtctgtt ttccaagggc atgtaacgca tcaggctttg 240 gtgggcactaatacaactgg gatatctgag aagtatagga catactctat tagagacggn 300 aaagatggcaaatacctgcc cgtttattct gtgtgactca ctggggctga gtgagaaaga 360 aggcggnctgtgcagggatg acatattcta tatctttgac ggtaaccatt cgtgatagat 420 nccagtttaattcccatgga atcaaatcaa attaaatcat catgactacc ttggttcccc 480 atcggttgaagggacngnat tcattggggn ggcattggat ttgatnncna gntttattca 540 atactttctc n551 123 575 DNA Homo sapiens misc_feature (1)...(575) n = A,T,C or G 123acttaataca tattttcaaa cctgtttgca tttcaaacaa agttagcgtt tttgtaaatc 60aaatttgata acccgactaa aaatattttc cagctttatt atttaaggag ctgcacagcc 120tttaaagtgg ggaccaggag gcaggcagag gcagagagac tgaatgcacc caggactgcg 180cagcagtcta cagcaacatg tcccacaact ttggtgctgg aaacacaagt aatgcacaag 240acagctgccc tccagtgtca ggatcctgtg aaacagcata tcaaaagatc gccagcttct 300tataatttac acactttcat ttaggattgc ttttttgaag aaaaatcttt aagaatgcca 360tttttaattt aatatccaga accctggaat ttaaaaaaac ctaatngaaa ggaaattaac 420tggtaccatc aaaaatgggg ntgntggttg gancntgtgt gaagttaggg aattctatgg 480ctttttttaa gatgccccgg aaaatttaac cccttaatng cangtttaat ttngaattcn 540cnccaggtan tgtatgtnng gctcanatta gtanc 575 124 570 DNA Homo sapiensmisc_feature (1)...(570) n = A,T,C or G 124 actgagacaa tggttagggttgttttctta attcttttcc tggtagggca acaagaacca 60 tttccaatct agaggaaagctccccagcat tgcttgctcc tgggcaaaca ttgctcttga 120 gttaagtgac ctaattcccctgggagacat acgcatcaac tgtggaggtc cgaggggatg 180 agaagggata cccaccacctttcaagggtc acaagctcac tctctgacaa gtcataatag 240 ggacactgct tctatccctccaatggagag attctggnaa cctttgaaca gcccagagct 300 tgcaanctag ccttacccaaaangactgga aangagacat atctntcaag cttttttcag 360 gaangcgtnc ctgggaatccaaggaacttt ttgatgctaa ttanaaangc ttgggactta 420 aaaatgtccn ctanggngtggcacttttac angtttttgg aangcttnga aggcaganng 480 gggtcnaana ntnaaaanacnnttgacntg ntaatanngg aatantangg cnaatggaaa 540 ctgngttggg ggaggatcaatttaaagagg 570 125 593 DNA Homo sapiens misc_feature (1)...(593) n =A,T,C or G 125 ggtacagaga tttaaatgaa atcttcgaaa gaataaattt gcttttcagtccactgtatt 60 ttcaaaattg attatcacca agcttggatg aaagctgtga accacaaaccatttgtttat 120 ttaatagaaa aaagaatgtg tagattatta gcaaagtaat gccttaaaatgtatcttcac 180 acagttgaaa ttttagtata aacttgtata tcaagttgct ttccattatttattctactt 240 taaaaatata tacaactatg atgttcaaat atgtattctg agccattatgttcaaacata 300 aatatctggg aaattcaaac tgctgcaaca agttaggaaa ggattaaggaaaaatgatga 360 gctacaaatt atgtagttgg aggaagaaaa aaatgttact tagcatttatgtctggatag 420 gtatgtattt tctaatttac atacacatat ccagttgagt atagaccaccatcaaaatgt 480 accagttaca cagagactag actaaaccac cctatttcta tacaggtaccatagtggatt 540 caaaaattta atatctcata gttcccaaaa ttattgnggn aatatgctnattt 593 126 592 DNA Homo sapiens misc_feature (1)...(592) n = A,T,C or G126 acgcgggggg gccttccggg acgagggcgc gtgggtgagg aaggtcaggt ctaggaactc 60taactccttg ccactcaaga aatgtcctcc ctttcagaat atgccttccg catgtctcgt 120ctcagtgccc ggctatttgg tgaagtcacc aggcctacta attccaagtc tatgaaagtg 180gtgaaactgt ttagtgaact gcccttggcc aagaagaagg agacttatga ttggtatcca 240aatcaccaca cttacgctga actcatgcag acgctccgat ttcttggact ctacagagat 300gagcatcagg attttatgga tgagcaaaaa cgactaaaga agcttcgtgg aaaggagaaa 360ccaaagaaag gagaagggaa aagagcagca aaaaggaaat agtgttggtc ccttcaagag 420ggagactttc ttcctaatgg ccggaaagaa gaaagtgcat ttattggctt tccacatatt 480ggaggaatgt catcttccta aatgaagttt atttggagga acacagtcat ttccttggtg 540aaactaatcc ggtacattgn ggttgggttt ttgaacacat ctactgggca aa 592 127 600DNA Homo sapiens misc_feature (1)...(600) n = A,T,C or G 127 acagtggtccttttcagagt tggacttcta gactcacctg ttctcactcc ctgttttaat 60 tcaacccagccatgcaatgc caaataatag aattgctccc taccagctga acagggagga 120 gtctgtgcagtttctgacac ttgttgttga acatggctaa atacaatggg tatcgctgag 180 actaagttgtagaaattaac aaatgtgctg cttggttaaa atggctacac tcatctgact 240 cattctttattctattttag ttggtttgta tcttgcctaa ggtgcgtagt ccaactcttg 300 gtattaccctcctaatagtc atactagtag tcatactccc tggtgtagtg tattctctaa 360 aaagctttaaatgtctgcat tgcanccagc catcaaatag tgaatgggct ctcttttggc 420 ntggaattccaaaacntcag agaaatggtg tcatcaagga gaaccttcat aaccccntga 480 anggattaaaaagccccaaa tggggggaac tgataatagc acttaaggct ttaagaattg 540 gncacantttcaccttgtga acccattnna cnatngngcc taanngctnc ctnctncaan 600 128 588 DNAHomo sapiens misc_feature (1)...(588) n = A,T,C or G 128 ggtacttttttttttttttt tttttttttt tttttttgag acggagtctc actctgtcac 60 ccaggctggagtgcagtggc atgatcttgg ctcactgcaa gctctgcctc ctgggttcac 120 gccattctcctgcctcagcc tcctgagtag ctgggactac aggcgtccgc caccacgccc 180 agctaattttttgtattttt ggtananaca gggtttcacc gngttagcca ggatggnctc 240 catctcctgacctcgtgatc tgcccacctn ggccttccaa agtgctggga ttacaggcat 300 gagccacggcgcctggccag gatggtatat ttttaactcc ttcactgggc cccacccctg 360 actttctgctttangaggtc tggggtgagg ctgaanatct gggggccaca cttcgagagc 420 aaccaagactgtaagtgggg ccttccanag cccaatgaag ggaatactta ggtacaggan 480 gtgtctgcatggncncangt gtggggtttn cttctcggcc ttaaccagaa agtatctctg 540 gttttaattttaaaatgaaa attttaaagg gtgnctgaaa cnaattgg 588 129 588 DNA Homo sapiensmisc_feature (1)...(588) n = A,T,C or G 129 ggtactgccc tctccagatcagcagttcag gagagcacag gaggcaaaac acagattgct 60 gggcttattg gtgccatcatcgtgctgatg gtcgttctag ccattggatt tctcctggcg 120 cctctacaaa agtccgtcctggcagcttta gcattgggaa acttaaaggg aatgctgatg 180 cagtttgctg aaataggcagattgtggcga aaggacaaat atgattgttt aatttggatc 240 atgaccttca tcttcaccattgtcctggga ctcgggttag gcctggcagc tagtgtggca 300 tttcaactgc taaccatcgtgttcaggacc caatttccaa aatgcagcac gctggctaat 360 attggaagaa ccaacatctataagaataaa aaagattatt atgatatgta tgagccagaa 420 ggagtgaaaa ttttcagatgtccatctcct atctactttg caaacattgg tttctttagg 480 cggaacttat cgatgctgntggctttagtc ccttcgaatt tacgcaagcg cacaaacttt 540 gaggaaaatc cgaaactgcnaagcaagntt gntacaagtg acccaaan 588 130 190 DNA Homo sapiens 130ggtacaaaaa aaaccttaca taaattaaga atgaatacat ttacaggcgt aaatgcaaac 60cgcttccaat tcaaagcaag taacagccca cggtgttctg gccaaagaca tcagctaaga 120aaggaaactg ggtcctacgg cttggacttt ccaaccctga cagacccgca agaccccgcg 180tacttttttt 190 131 386 DNA Homo sapiens 131 ggtacagaac tcagaggaaaaaagaaatta aattttagct ttctggagag cagcccctct 60 ctggcaccat caaacacttctttgtttccc ttcaacttgg aactcttcaa acatcagggg 120 ttgtgagggt ttggccattcttttatcttg ggtccatgtg agtgacagaa atggtgcggc 180 ctgggaaaga tctccctcctttacattttc tcttctccct cctcctcctt attctaaaac 240 tgtgcctcca acagaggggcaggggctctt gtagagagat ccctggccca ggacaggaga 300 tgccaaatct aatttatctcactgagggcc tttgagaaaa acgcttcagg gccaggctca 360 gtggctcatg cctatataatcccagt 386 132 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,Cor G 132 actgagacaa tggttagggt tgttttctta attcttttcc tggtagggcaacaagaacca 60 tttccaatct agaggaaagc tccccagcat tgcttgctcc tgggcaaacattgctcttga 120 gttaagtgac ctaattcccc tgggagacat acgcatcaac tgtggaggtccgaggggatg 180 agaagggata cccaccacct ttcaagggtc acaagctcac tctctgacaagtcagaatag 240 ggacactgct tctatccctc caatggagag attctggcaa cctttgaacagcccagagct 300 tgcaacctag cctcacccaa gaagactgga aagagacata tctctcagctttttcaggag 360 gcgtgcctgg gaatccagga actttttgat gctaattaga aggcctggactaaaaatgtc 420 actatngggt gcactctaca gtttttgaaa tgctaggang cagaagggcaaaaataaaaa 480 acatgacctg gttgaaggaa naaaagcaaa gaaacttggg ngggaggacaattaaaaaga 540 gnncctggga tcccctnttc ttaggtccct ctcttacnaa ggacnctntttat 593 133 588 DNA Homo sapiens misc_feature (1)...(588) n = A,T,C or G133 acagancatt nnnagcnctn gcacaggnta cagaacctna cagacccaaa ggaacatcgg 60ataggcnaag cgactacagg aggcgtgtgt gcgcttgggc naggtaaaca gggtcagtat 120tggtcnngtg acaagagnca cgaantctgg ccngacantg angtnaanaa ggttnatnnt 180ttnacantta tnnnanatat nnnnnaannt attaanctgc ancanntgat tttnacacct 240anttactaga aaactaanga aagcactnat tagctctgaa tnaantnaca tggnaagcct 300tttactaatc tncaaanaaa ccttctctgc antatnnnaa agattttatn atacaangng 360gnnnatcnct cnatcatann gggttctatt ananaaccct gctaantntg cgacttacag 420aacanccagc ntananatga ntttcatgcc catttgggaa gcatngcccg ggtatcacaa 480aggaaaccta ctaaagnttt ctgttatacc agccttcntt cntatcantg catgngnana 540nanaaccntt gaaggttntc cnggggactt tnttctnttn ctttgccc 588 134 618 DNAHomo sapiens misc_feature (1)...(618) n = A,T,C or G 134 tcnagcggccnnccnggcag gtacantcac annttnnang anctnaacac anactanctg 60 nngtcaaatattnaacaaaa gcantagatg aanctgctta acattcacgg aaaaacaacc 120 aaaagaagggaggggtgata aaccanaaaa atgantgacn aaaactaaga gacctcatan 180 gngtctttacaatcnggaat tcagatgcaa ggaacagacn caaanctgtc taaaatgtna 240 cctatgaggcnacanaaagt gacttaaagt ctggtntnan taaaaaatga caacccttat 300 cctagagagtcttacnttat ttaatccana cnttatntaa cgccncngat ttttgnttgg 360 ngctatggngttnattttnt atcagaanga antgtgggac anatgcatta ctgnttgttn 420 aaagngcttnacagctaatt cacncccnng ggcatggtca aaaaggnaan aaccnggnca 480 tatattgntganatgaaaaa accacntgtt aaaaaaataa ntgnagccna ntgngttttn 540 natgataaccaaatnttnac nttcagtann ngccttttan aagttggtga actccgaaat 600 ctncttttttaaaccngg 618 135 374 DNA Homo sapiens misc_feature (1)...(374) n = A,T,Cor G 135 actttttttt tttttttttt tttttttttg gggatggagt ctcactctgttgtccaggtt 60 ggagtgcagt ggtgtgatct cggctcactg caacctntgc ctcccaagtgattctcctgg 120 ctcancctcc tgagtagctg ggactacagg catgcactac catgcccggctaatttttgt 180 atttttagta nanacagggt ttcaccatgt tggccaggct ggtcttgatctcctaatctc 240 aggtgatccg cctgcctcan cctcctaaag tgctgggatt acaggcatgagccactgtgt 300 ntggccaana ncactcgtaa gaaggatggc agtatcacaa aatcaagccagagatacaga 360 gattacccgc gtcc 374 136 581 DNA Homo sapiens misc_feature(1)...(581) n = A,T,C or G 136 actccagcct tgctgaagct gcctcaaaggctgatggttt ggcagttatt ggtgttttga 60 tgaaggttgg tgaggccaac ccaaagctgcagaaagtact tgatgccctc caagcaatta 120 aaaccaaggg caaacgagcc ccattcacaaattttgaccc ctctactctc cttccttcat 180 ccctggattt ctggacctac cctggctctctgactcatcc tcctctttat gagagtgtaa 240 cttggatcat ctgtaaggag agcatcagtgtcagctcaga gcagctggca caattccgca 300 gccttctatc aaatgttgaa ggtgataacgctgtccccat gcagcacaac aaccgccaac 360 ccaacctctg aagggcagaa caagtgagagcttcattttg atgattctga gaagaaactt 420 gtncttctca agaacacaac cctgcttctgacataatnca ataaaataat aattttaaaa 480 aataaattat ttcaatatta ncaagacacatgccttnaat natctgtaaa ctaaaaacta 540 aaatttantc tactgnttaa tcnaanataataatagcttc a 581 137 504 DNA Homo sapiens misc_feature (1)...(504) n =A,T,C or G 137 ttttncaaan nnaagttttt tacttccnaa aantnatggc taaggggngggnggngggng 60 aaaaaagnaa aacaaaaaaa ccccaaaaaa atggggnggn naaaaggggggganaaaaaa 120 ccnntntttt ntaaantntn acaaggcaag ngcnnangga aaaaaaaaanncctgnaaaa 180 tcccccncgg nnggggnaaa natnnnggtt tccttttgnt ttnaaacccnntnangnaag 240 gntntccccc ntncccctna atnaaaaatt tntntnccng ggccnnaaccnccntanggg 300 naaattccac cncnctgggg gccgttanta agggatccna gctnggcccaancttggnga 360 aacatggcaa aactgttcct nnggnaaaat gtttcccctc anaattcccanaaaataaaa 420 ccggaacata aagngaaaac cngggggcct aagngggncn cacnccatttattggggtgg 480 cccncgnccc tttcaaangg aaac 504 138 386 DNA Homo sapiens138 acaacaaata acactgtgac tccaacctca caacctgtgc gaaagtctac ctttgatgca 60gccagtttca ttggaggaat tgtcctggtc ttgggtgtgc aggctgtaat tttctttctt 120tataaattct gcaaatctaa agaacgaaat taccacactc tgtaaacaga cccattgaat 180taataaggac tggtgattca tttgtgtaac tcactgaagc caaaatacta tcttttaaga 240tgtcccacat ggaagacgct attccaggat ctttaaattt ccatggatgc atataggatg 300tttgggagca tcatccgtga agaaaaaatc aattaaatca ttgtgttcaa caggaatatt 360taaaataaaa aaaaaaaaaa agtacc 386 139 586 DNA Homo sapiens misc_feature(1)...(586) n = A,T,C or G 139 ggtactcaag tttataatgt ccccaaaccttaagactaga aaatcatccc aagaaaaagg 60 cctatagttg gtttaatttc accctgagaatactgtgata aaaatcaata tatttcagag 120 ctagtaagta tttaaaaatt agtgtctcaaaaaggggaca tcataaggga aatacagggt 180 ttagaggtct gagctcaagt ggtgtaagacagttctttct tcttcctcct ttaaactctt 240 cactttgctc taacacggaa gatgggggacagtgatcccg aaggtattac taaaatattg 300 cagctttcag taattatgag aagcacagatatcaccagaa aagaaagcaa tcatttggag 360 tactaagaaa cgaaacaatg ttatttggtggtgtataatt ctacttttct agtagattac 420 tgngtggaat tctgtgaaaa atatttgagaaaangcctgt attgcataaa taaatctttg 480 tatgttgcaa aaaaaaaaaa aaaaaaaagtacctgccggc cgncccaang gcgaattcca 540 cacctgcggc cgtctagngg tccacccggtccacttgggt atatgg 586 140 591 DNA Homo sapiens misc_feature (1)...(591)n = A,T,C or G 140 acagggagga atttgaagta gatagaaacc gacctggattactccggtct gaactcagat 60 cacgtaggac tttaatcgtt gaacaaacga acctttaatagcggctgcac catcgggatg 120 tccctgnacc aaccttcaag gccnaaaccc nnntggtgnntttggnctnt aaatnaggat 180 ggccctgtnt tccntaggta acttgttccg ttggtcaagttattggatca attgagtata 240 gtagttcgct ttgactggtg aagtcttnac cnngtccntttngngtgggg tttttttagg 300 naaaagnctt ttggtncatt nntggggggg gnaggggactgaacctttat tntttccaaa 360 tncaccttaa antcagggac aanaaacatt ccaanaaccacaatctttta aaaaattaac 420 tngccagtgg gaatgtttaa aaanntnaan ggtcttttttgccttggttt ttgtgggggt 480 ctctcttccc ccccctgggg ttaatttttn aagccgggacctcncnaana cccctttttt 540 caaagggccc naaacccccc cccccnaaaa aaaaaaaaaaaaaaaaaanc n 591 141 592 DNA Homo sapiens misc_feature (1)...(592) n =A,T,C or G 141 ggtacacaaa ccaagacaat atcagggtga caggtgaatg aacttaaattctcagtcttg 60 tctattcacc aaaaaagtat actgcctgtt ttttctttaa ttattcaaggttgatgactt 120 ttaggaacat gttttatact gtatttttta attaaagcaa gtgccttgatgtaattccat 180 gtaaatcatt gcttaaccct cttatgggat gaggatgagt tattaatgtattgcagccta 240 ctggaaagga gggggagttg gttaatagca gatacttttc ttctagaagcttatgtttta 300 tgctgtttat tatgtaagat cctgtatgtg tgttgagatt tagaggtttcatttgttttg 360 tctgctaata aattgttact ctaataataa ccnngnnaaa naaannnnnnnnnnnnnnnn 420 nnnannnggt ncctgcccng gcggccgctc gaaagggcga attccanccactggcnggcg 480 gtactaaggg gatccgnctc gggncccaac ttggcgtaat atnggcatactggttcccgg 540 gngaaatggt atncgtcaaa ttccccaaat acnaccggaa ncttaagggt aa592 142 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 142acaacacctt cattcttaat gcttcttagg gcatcacagg ttttagaaat taatgtattt 60ttagcattcc acagtaatga tcactttcaa aaactgcaat atacatctgc atgttacact 120gacatacaac acataagtat tttgtcacac atcaactttt agcctcaaat aatagaatac 180aaaaagctac actggacata acaccaccga acttttgaat atcccctttt cccaattgtt 240aacaggtagt actgggatta caggcgtgag cctctgcgcc tggccaagtg gaggttatta 300ttaaccctat ttaacagata taaaaagaag agattagaga attttatcaa tgttcccact 360gtcaaataga atataagcaa tgatacaaaa tgttgagtct tcatcctcta actccagatc 420ctggtatatt gccctacatt tctatacatt aatactaact tatacactga atacaagagt 480naaaccaact gtcngggctt aatanggnga aaatgctctt gncctaaanc accagggtgg 540ctnggtttat tcctacatgt ggactaaaan gnaatcatct ttatggcngg aaana 595 143 620DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G 143 actactcgattgtcaacgtc aaggagtcgc aggtcgcctg gttctaggaa taatggggga 60 agtatgtaggagttgaagat tagtccgccg tagtcggtgt actcgtgtga agttggcagg 120 gacggttcctgtcatcttct tgggcttatt tggtgtgctg ttgaaggggg gagactagag 180 aaatggcagggaacctctta tccggggcag gtaggcgcct gtgggactgg gtgcctctgg 240 cgtgcagaagcttctctctt ggtgtgccta gattgatcgg tataaggctc actctcccgc 300 cccccaaagtggttgatcgt tggaacgaaa aaagggccat gttcggagtg tatgacaaca 360 tcgggatcctgggaaacttt gaaaagcacc ccaaagaact gatcangggg cccatatgct 420 tcgaggntggaaanggaatg aattgcaacg ttgtattccn aaagaagaaa atggttggaa 480 gtaaaatgttccttatgacc tcncaacctt ataaacncat ccgtttnttt acaaccntta 540 accacatgggaagttcattn aaaaaaactg aaaactttgn aaagnttttt ttnnccttga 600 aaagggaacttacctcgccc 620 144 613 DNA Homo sapiens misc_feature (1)...(613) n =A,T,C or G 144 cgaggtactt tttttttttt tttttttttt ggggtcagtg gtgatatccccctaatcaat 60 tctgattgng ttccttttaa tcttctctca tttctttttt attagactagatagtgattt 120 atctatttta ttaatttttt caaaaaatca cctcctanat ttgttgttttttaaggggtt 180 ttatgtctct atctccttca gttcaactct gatcttggnt atttcttgncttctgctaga 240 tttggggttt gntttctgnt ggntctctaa gttctttttg ntgngacattagattgncaa 300 cttaaaatct ttctagctat ttgacgtggg catttaatgc tataaatttcctggtaacac 360 tgctttcgct gtatnccana naatctggga tggtggggcc ttggtttcaataanttccaa 420 tacctcttaa gggggnggag ccaanaagan ctaatagggg cagcactgctctgggctncc 480 atcaanaagg acaaaaactg ggagngaccc tgcttnttca ctgaggnaccggcccggccg 540 gccgtccnaa ggcgaatcca cncnctggcg gccgtctatg gatccacccggnccaactgg 600 ggaatatggc aaa 613 145 345 DNA Homo sapiens 145acactgatct acaaaaattt taaaatgagc cgggcgcggt gactcacgcc tgtaatccca 60gcactttggg aggccaaagc aggcggatca tgaggtcagg agatcaagac catcctggct 120aacacggtga aaccccgtct ctactaaaaa tacaaaaaat tagccgggtg tggtggcggg 180cacctgtagt cccagctact cgggaggctg aggcaggaga atggcgtgaa gccgggaggt 240ggagcttgca gtgagccgag atcacaccac tgcactccag cctgggcaac aaagcaagac 300tctcaaaaaa gaaaaaaatt tttttttaaa tgagctgggt gtacc 345 146 475 DNA Homosapiens misc_feature (1)...(475) n = A,T,C or G 146 actacaaggtttagcatttg ctctgctggt cgacattccc ccagtctatg ggttgtatgc 60 atcctttttcccagccataa tctacctttt cttcggcact tccagacaca tatccgtggg 120 tccgtttccgattctgagta tgatggtggg actagcagtt tcaggagcag tttcaaaagc 180 agtcccagatcgcaatgcaa ctactttggg attgcctaac aactcgaata attcttcact 240 actggatgacgagagggtga gggtggcggc ggcggcatca gtcacagtgc tttctggaat 300 catccagttggcttttggga ttctgcggat tggatttgta gtgatatacc tgtctgagtt 360 cctcatcagtggcttcacta ctgctgctgc tgncatgttt tggtttccca actcaaattc 420 atttttcaattgacagtccc gtcacacact gatccagttt caattttaaa agacc 475 147 629 DNA Homosapiens misc_feature (1)...(629) n = A,T,C or G 147 cgaggtacgcgggatttgaa tcttaaactg tatttttctc ttagtattgc taatgagtaa 60 agaaaagtctcataaggtag ccaaatgaaa aagaatgaaa gggaaagtga aaaattaagg 120 ggacaaaagatgggatgtga aaagaagaat tctagtttga tggtgactca tattcacgat 180 aggatacaaagtgtgatttg ttggaaacat gtcccaaatt tctaaaattc tgcttctctg 240 ccaaaagcaatgtctttctt ggttgatatt tgagttttaa aagggtcaaa tctttctaat 300 tttttgtatctttagagggc agcactagaa gaaatcagca ggtctaatcc caccagtaag 360 aaaactaccacttcttgatt tttacagatt taaaaaaatc ttttcagtgc ctttcttttt 420 aatgtaaatacaaatttaaa cctangctta atatagcgtt tccctttccc caagtgatgt 480 cnaggtcgatgccaaatcaa tgatccnaaa tgatcgnggt naaaataact caaagggttc 540 ttaaggngagtngcatgcca aaaaatacct tgattccggg ggtttggacc tggctttgtt 600 ggggcctntgaaatgccaan ttancccan 629 148 614 DNA Homo sapiens misc_feature(1)...(614) n = A,T,C or G 148 acaaaagagc ctgattcttt ttaattccacaaatacctag catctcaaag taacatgtaa 60 acaaacttct atgctgctca atgaatccttccaatttcga taataaacta aatagtattg 120 gatctagtat atgactttca tgtgtaagttatggttctat ccattacttt aacaatatta 180 ctgatgtaac agagaaaaat tttcaactattgtatttatt taaaacaaac tgacaagttc 240 aagcacctgt cttcagaaaa gccagcagcatttttttttt ttaacatact caaagtaaga 300 tttggcctaa gcccttaata cctttctgaacagccatgca actaaacacc ctcagggaga 360 tgttacataa gggagagaag aacatggagcaatttgcact ttttccctag ataatattaa 420 caaggnaaag caaatncaga tctttatgaatgaatggntg gcatggttaa tcacttggac 480 tttttaaact agagncncta tcatattggtaaatagaaan aaaggatttt aataaagctc 540 tncctgcttc aaaattaagg ggacnttttctgggaggctt tcagggacca taataaggta 600 aaaggggacg gttg 614 149 628 DNAHomo sapiens misc_feature (1)...(628) n = A,T,C or G 149 nccgaggnactttnnttttt tttttttttt ttttnaacag cgncttttca tttttattac 60 tcaaaaaagtttcatttttt tatttaagct ttctgactct gngcttgggc cttcaacact 120 ttcacaacgattttctgctc ctcgataagg aaagcccgct tgatcctana aaggaaaata 180 ccaaattaatcatttcttta aaatgaactt cattttttat ttagcccaaa aaaggnaaac 240 atggtaaagaaccaagcnaa gcaatcaggg aacccaggaa actacnggat acccaaatac 300 ngagtaaaacttaaaagggg aaattcattt aaagcaggga aatccctcaa tttcatgccn 360 gtagttatctgncctcctct gagcaagaat aactatgaag catcccccag gagaccacnt 420 atgagacttaattattggta ggatccagga atagnggnat ttnttgattt gcaaaangtn 480 taaaaaattttaaccctntt ttgaaaattc ccagnaaaaa caccncataa ggggctntgt 540 gttaaaactaaaattaaagg gaagggtttt tccagaaacc ccccccanac cagggtttna 600 accggttanggcanntcncc aaaccnan 628 150 509 DNA Homo sapiens misc_feature(1)...(509) n = A,T,C or G 150 ttggggaann aaaaaaaaac ttttttttttnggggnnngg ggntgcnanc natncaaaaa 60 tcaaaancnt ntttgggttt taacttttttttttttgntt gncaaannaa aantaaantt 120 tntttttana tttgctaang ggccngancngcnnaaaaaa nccttttttn ggggaanctt 180 nggggcaaat tnnttnancn accctttggganaacttttn ttaggggggn nnnaaccgnc 240 atttttgccc acttttttcc cttttgnttaanggggncct tgggcnggac cncccttagg 300 ggnaattcac ccnctggggg gcgttatnttggatccactc ggnccaactt gggggaaaaa 360 gggaaaacnt tttctggggn aaattttttcccncnaaatt cccaanaana aaaccggaac 420 nnaaanttaa acccgggggc ccaaggngggccnncccntt nttgggtggg ccctgcccnt 480 ttaangggaa attttggccc tttttaaaa 509151 622 DNA Homo sapiens misc_feature (1)...(622) n = A,T,C or G 151ggtacttttt tttttttttt ttttttttgc tttggacaaa tttattgaaa catacaggcg 60gctgttagca gagaaatcat tccatgattg atgtgttaca tttggccact accttgaatg 120tataatttaa aaattatatt tttcacaact aagcctttgg ccaaaaaagt catttagcac 180atctttaaag atcaataaga aatggatttt ggacattaaa aagatcaagt cactgaatta 240aacagtagca acccccatta atctagaatc ccatagtgct gaaggtagag gtgtctgtgc 300aaagctagtc atttgttaac agcaatcana aaanatgggg gcaggcacac ctgtcaaaag 360tggcaacana nctggcagga caggacggct gggctggtct ggtcaggtga gcatgtncca 420aaaacagcag caacagaaaa cccgtccacc angcttgtga agcangtgga tggtcctagc 480tcatctnttn ttttggnctt ntancacata cactgngggt ttangangnt tctgaggncc 540accttgccnc cctacctgcc cgggnggccg ttnaaagggg aattccacca ctgggggccg 600tctaatggga cccacctggg cc 622 152 313 DNA Homo sapiens misc_feature(1)...(313) n = A,T,C or G 152 acggtggatt agttcttttc agcatgttccttctgtatga tacccagaaa gtaatcaagc 60 gtgcagaagt atcaccaatg tatggagttcaaaaatatga tcccattaac tcgatgctga 120 gtatctacat ggatacatta aatatatttatgcgagttgc aactatgctg gcaactggag 180 gcaacagaaa gaaatgaagt gactcagcttctggcttctc tgctacatca aatatcttgt 240 ttaatggggc agatatgcat taaatagtttgtacgcgggg aaaaaaaaan aaaaaaaaaa 300 aaaaaaaagt acc 313 153 620 DNA Homosapiens misc_feature (1)...(620) n = A,T,C or G 153 cgaggtacgcgggagggcaa caagaaccat ttccaatcta gaggaaagct ccccagcatt 60 gcttgctcctgggcaaacat tgctcttgag ttaagtgacc taattcccct gggagacata 120 cgcatcaactgtggaggtcc gaggggatga gaagggatac ccaccacctt tcaagggtca 180 caagctcactctctgacaag tcagaatagg gacactgctt ctatccctcc aatggagaga 240 ttctggcaacctttgaacag cccagagctt gcaacctagc ctcacccaag aagactggaa 300 agagacatatctctcagctt tttcaggagg cgtgcctggg aatccaggaa ctttttgatg 360 ctaattagaaggcctggact aaaaatgtcc actatggggt gcactctaca gtttttgaaa 420 tgctaggaggcaaaaggggc agagagtaaa aaacatgacc tggtagaagg aanaaagcaa 480 aggaaactggtggggaggat caattagaga ngaggccctg ggatccncnt nttcntaggn 540 ccctctcatacnaaggacac tttttatatg ccttcccaaa ctgntnggga agggtnaaac 600 caaaatccggggtanaacct 620 154 339 DNA Homo sapiens 154 ggtacctgga ggatatagacctgaaaacac tggagaagga accaaggact ttcaaagcaa 60 aggagctatg ggaaaaaaatggagctgtga ttatggccgt gcggaggcca ggctgtttcc 120 tctgtcgaga ggaagctgcggatctgtcct ccctgaaaag catgttggac cagctgggcc 180 gtccccctct atgcagtggtaaaggagcac atcaggactg aagtgaagga tttccagcct 240 tatttcaaag gagaaatcttctggatgaaa agaaaaagtt ctatggtcca caaaggcgga 300 agatgatgtt tatgggatttatccgtctgg gagtgtggt 339 155 450 DNA Homo sapiens misc_feature(1)...(450) n = A,T,C or G 155 cgaggtactt tttttttttt tttttttttttttttcntat ttttgtttaa tttatttaan 60 accacctnct tacaacttnc anagagaaaatacaaaacaa gaaacanact tggtttnaaa 120 tgcataacca gntgctggan tttaaagcattactgataac attgttacan aanaatggca 180 nnttactcna gggcacttna gtattcctnaggaataaaca ttgatttctc ttgtcctccc 240 nntgggatgt tctcangtna agtcactgcncctgcnctta gacatatttt ccatgtnnca 300 naananggag cctgnaaant atgctnacagtnggaataag ccattnctaa ttccatgcca 360 naaccnangg ctaatggnnc attcttttttaataaggtat gtggaaaana ttcntatccc 420 aaanaaaant tgcccggncg gtctntntaa450 156 760 DNA Homo sapiens misc_feature (1)...(760) n = A,T,C or G 156cgaggtactg ccccagtgaa aatggaactg aaagagcctg tagctgtcag agaaaggacc 60acctttcagc actgatcggt tatcgttgtc ctcaaaattt acatggaagg aatgccccac 120attgataatt tctttggctg tggctgggtt gtaggagaca ctaataggtt tcagagaggt 180gtcatgtttg gtttcactgg ttttaatatc aacaggggac tggttatttc cattggcaat 240gggatacagc ttgctccatt gttcaggacc atttttgtca tcatatcccc agtctggact 300tgccattatc ttctactgag ttttcttttt ctgaaaacaa aaataatacc tggaataact 360aactgccccc gcgtcctgcc cgggcggcca aaggggcaat tccaccactg gcggccgtac 420ttatggatcc aactcgtccc ancttggcgt aatatggcat aactgttctg nggnaaatgt 480atcccttaca attcccncac atcnacccga acctaantgt aancctnggn gcnnataagg 540actactnctt aatgggtggc tctgncnttt caannggaac cttngcnctn gntatgattg 600ccaccccgga naggggtggt ttggccttcc ntcttgtann aatcttcncg gnttgttgga 660anggtnntct taggggatng ttccaatggg gaccgnaanc ttccagccna ggcaccaaan 720cnttggttta ncccccacnn aaaantanag gggncngggt 760 157 668 DNA Homo sapiensmisc_feature (1)...(668) n = A,T,C or G 157 ggtacccagt agtcattcaggaacaggttg ttcagtttcc atgtagttga gcggttttga 60 gtgagtttct taaacctgagttgtcgtttg attgcactgt ggtctgagag acagtttgtt 120 ataatttctg ttcttttacatttgctgagg agtgctttac ttccacctat gtggtcaatt 180 ttggaataag tgagatgtggtgctaaaaag aatatatatt ctgttgattt gaggtggaga 240 gttctgtaga tgtctattaggtctgcttgg tgcanagctg agtcaattcc tggatatcct 300 tggtaacttt ctgcttgttgntctgtctaa tattgacagt ggggcgttaa agtctcccat 360 attattgtgt gggagtctaatctctttgta ggtctctaag gacttgcttt ataaactggg 420 tgctcttgat tgggtgcaatatatttagga tagttagctc ttcttggtga atggancctt 480 taccaatatg aatggcctccttccttttga ccttgtgggt taaagctggt tatngaaact 540 ggatggancc ctgctttttttggttcattt cttgnagggt cctcagcctt attttancnn 600 gnggctttgn cccncntccgcggcnttaag ggaaccacnc tgngcgtcta ngancactgg 660 caactggg 668 158 737 DNAHomo sapiens misc_feature (1)...(737) n = A,T,C or G 158 tttttttaagggtcaatgtt tacatttttt tcatataaat atcaagttgt cagcaccatc 60 tgttgaaaaaaatctttgta atggctaatc ttttatgtca ttagatttga taatagttta 120 agaatttttgttcctatatt catgagggtt gctttccttt aacttttttg ttttgtaatg 180 tctgtgtcaggntttactat tagaacaata ctagtctagt aaaaaaaaaa anaaacaaaa 240 aactancaagtgtntctccc cttctattta taanaanggn gttacttctt ccttaaatgg 300 nnaaattatgagngaaactt ggagtatcnt tgcnggantg gaagtttcct tgtggaaaga 360 attttatnatnattacattt caatagtncc gcntccctgc ncgggcggnn ntcaaaggcg 420 aatncagcaaattgntggcc gntactnngg accaacntcg gnccatnntg gggnancang 480 tcaanctgttctngnnaatt gtncccttcc aatncccaca nanaaccgaa cctaaatgga 540 acccnggggctantaangnc taccnntatt gngnggctnn gcccttnnnt ggaaactgnt 600 cnaccnttataatggccccc cnggaaggnt tntttggcct tctnntncaa anctggcngg 660 nttntgtgnaggttatctna ntggatgttc cacgggaacn gaanatntan ncagtggacn 720 aaanntnntnttttnct 737 159 739 DNA Homo sapiens misc_feature (1)...(739) n = A,T,Cor G 159 cgagggtaca ctgtgagaga ataacatgga cttgatatgg catcacacttgttttaaagc 60 aaaaaaaaag aaaaaaagaa aaaaaagaaa gtacagttaa aaagtaagcattgtagtaaa 120 tagtggattc tctggtgtgt attttttatc tcagtgttga aaattggaaaagaatgggct 180 gaagtctaaa aactggaata atgaaggaca ctaaatgcct ttattgtagatactatgttt 240 gtaagtctat agctaagcaa cttaagccaa aaaggtcttt caactgaagctttaatcaac 300 ttattttgga gatgttctct tccttatctc atgcgtcatc cctaaaataataagatacat 360 gggatcaaat aacccttgcc ttttcaacac aaatcagttg gaaaattatgggttgagtcc 420 tgttgctgcc atggttctgt tctcaaaatg agtgtgtatg acatcccatctatgtaatag 480 gctacctttt tggctcttgg aactttgtcc tgccggccgg ccnttaaggcnantcnacca 540 ctggcggccg tactatgggn tccagctcgt ccaaccttgc tatcntggctacttttctgg 600 ngaatgtatc cgtncatccc cacttcancg gagctaangg aancntgggcctatggggct 660 actccatatg ctngccnctg cnttcnangg aacncgcntc ttaanatgcacccnggaagg 720 gtngtngcct tcnttcttt 739 160 802 DNA Homo sapiensmisc_feature (1)...(802) n = A,T,C or G 160 cgaggtacag cagagaccttcctgcttttt actggggact ccagattttc cccaaacttg 60 cttctgttga gatttttccctcaccttgcc tctcaggcac aataaatata gttataccac 120 taaaaaaaaa aaaaaaaaagtacgcggggg cccattgttt ttgtaatctc tgaggagaag 180 cagcagcaaa catttgctagtcagacaagt gacagggaat ggattccaaa caccagtgtg 240 taaagctaaa tgatggccacttcatgcctg tattgggatt tggcacctat gcacctccag 300 aggttccgag aagtaaagctttggaggtca caaaattagc aatagaagct gggttccgcc 360 atatagattc tgctcatttatncaatatga ggagcaggtt gactggccat ncgaagcaag 420 aatgcagatg gcagtgtgaagaaagaaaca tatttacctt taaagcttgg tcccttttna 480 tcgaccnaag tggtccgacaagcttggaaa attactngan aaagctcaat nggactatgt 540 gactcttttt aataatttccanggntttaa acccgtgagg acttttcccc cgntaaatgg 600 aaagtatttt gcnannggacttgacttccc ggngccntaa gngaattcac cactgggggg 660 gnttagggtc cnnntggncaanttggnaaa ngggtaatnn cntgnaatgt tcctcatccc 720 aantngccgn ataantaaccgggcaaaggg cccaaatggn gccctccttn nngaatnanc 780 cctntannna ancggggggg gg802 161 214 DNA Homo sapiens misc_feature (1)...(214) n = A,T,C or G 161acttttnntt tattcnttat ttttgggacc tgctctcact gtccacccag actggagtgc 60antggcacca ttatagctna ctgcagcctt gacctnntgg gctcaagtga tcctnctgtc 120tacacccccc aagnatgntg tgacattatg cttggataat acttgtatnt tangtaaaga 180cagggtcttt ccnatnnacc nggnagatct naaa 214 162 304 DNA Homo sapiensmisc_feature (1)...(304) n = A,T,C or G 162 acttaggaat acaactatatacatatgatt ttatttttaa gaccatatta tatttgggta 60 tctactaata ttttgtataaagcaattttt tgttccatta cgtgactttt tgttttattg 120 tatatgtaat ttaacacacaataaagggta aagttgcttc cccaaaccac acttttaatc 180 aaaacctaga atcatctgcagtccttgtta aaaatgcagg tttctagaac cctctgaagt 240 tctgattaaa taaatttattgcaaatcaaa naaaanaaaa aaaaaaaaaa agnccccggg 300 gnta 304 163 461 DNAHomo sapiens 163 actagagcca gtcatcctta acaaatcttt tcacatttta tttctttcacatgtagtcat 60 cttcaaaaag gaaagatttg gaattttaga aaaggggcaa ctcttctttttagcattctc 120 atcagaaagt cacaaaaatc gatggaatca tttccactgg gaagattgaccttttgtatt 180 tatttgtggg gtaaattaat aagcattcca gatgcttgca gcttcctgcatccaggagat 240 gctgtgttcc ccgtgatgca gctggaaccc aagctgcagc aggagatgcaagtttcagga 300 tgttccccac tgagctggag gaatatctac agcagtgatg cttgaaattttgtatgaatt 360 attttgtcgc ctaccctttt cctccaaaca aaaattagag gattatttaatccttgggat 420 cttccccttt ttgagaaata aagtttttat caaaaaaaaa a 461 164 345DNA Homo sapiens misc_feature (1)...(345) n = A,T,C or G 164 tttttttgagacaaggtctt actctgtcac ccaggctgga gtgcagtggc atgatcttgg 60 ctcactgcaccctctgcatc ccaggttcaa gtgattctcc tgtctcagcc tcccttgtag 120 ctgggattacagccacttgc cactgcaacc ggctaatttt tgtattctta gtagagatgg 180 ggttttaccatgttggccag gctggtcttg aactcctgac ctcaagtgat ccacctgcct 240 ccatgtccaaagtgctggga ttacaggcat gagccaccac ccctggccta agtcattaat 300 ttaaaaaatgttattaggat gancgacctg ccgggcggcc gntaa 345 165 385 DNA Homo sapiensmisc_feature (1)...(385) n = A,T,C or G 165 actgaaacag aaactntacccaattgcagt ccatatgttt tctgggatcc cggagttccc 60 tttcaacaat gtaaaatacanacttaggtc aaaagttccc atgtctgaga aaactcaagc 120 caaatcagtt ctcctccaaagttgacagga tttatgcttt aaaaatagag atacagaatt 180 ctctttggaa agatctaccaaattcctgta agaaacagtc tacccaaagt aggggaaagg 240 ctatatgana agttcaaggcacttcttaaa aatatatctt aggttttagg gaaaggaaac 300 agacaagttt ccagacccgtgggtggaatg gatgtagcag atcactgaga ggttacaagc 360 gccgacctng gccgngacacgctan 385 166 745 DNA Homo sapiens misc_feature (1)...(745) n = A,T,C orG 166 tttttgacga tgtctctcaa caatacctga agtttctcat actcatcatc ccaagtctga60 aaaacttcaa agcatgctac cataactttt tcaaattctt cataagcaac atgcatcaat 120ttcctagtgc ccaatacttt gagtaattga gaactcaagt ctcttgaaat tgcctccacc 180aaacgcagtg ccctctgaat aggatatttt gtgtttcgga tctttctcaa atcccgcgta 240ctttgagaag ctgaggcggc agatcacttg aggccaggag ttcgagacca gtctcgtcaa 300catggcgaaa ccctgctcta caaaaaaaaa aaaanaanaa aaattagcca gacatggngg 360cccacatctg tagtcccagc tacttganan gctgaggcat gagaatagct tgacctggaa 420nggcaaaggt ttantgancc caaactgngc ctggattcca atnnggngga cccagtgana 480tttgtctcaa aaaaangaaa ggaaaaaaga gcccgncgga aggaaggatg gattgangga 540aaattgtggc ctccnnnnaa aggnccaang gccctnangt ttctttgaat agtttccctn 600gccnttctta ngggcctnng ccttttttcn nnctggcgaa cctaggnatt cacatggggg 660ttangacncc gccnctggga naggaaagtn ctggaagnnc ncntcccaat ancgnntang 720aacgggcngn ggannaattt tttnc 745 167 623 DNA Homo sapiens misc_feature(1)...(623) n = A,T,C or G 167 accagccact gcaaaaacat gccaaattgtaaagaccatc gaggctggga agaaactgca 60 tcaactaacg agcaaaataa ccagctaacatcataatgac aggatcaaat tcacacgtaa 120 cactattaac ctgaaatgta aatggactaaattctccaat taaaagacac agactggcaa 180 attggataaa gagtcaagac ccatcagtgtgctgtattca ggagacccat ctcatgtgca 240 gagacataca taggctcaaa ataaaggaatggaggaagat ctaccaagca aatggaaaac 300 aaaaaaaggc aagggttgca atcctagtctctgataaaac agattttaaa ccacaaagat 360 caaaagagac aaagaaggcc attacataatggtaaaggga tcaattcaca agaagggcta 420 ctattctaaa tatatatgca cccaatacaggacccccaga ttcatgaagc aaatccttga 480 gattnccaaa ggattaactc cncccngtattatggagact tncacccact ntnacctttc 540 ccgatcttgn cccaaagtac cnggtttcccgaattgactn gtttgncann gggctattaa 600 tttngaattt cncccaaaaa aaa 623 168703 DNA Homo sapiens misc_feature (1)...(703) n = A,T,C or G 168ggtactccct gtttgctgca gaatgtcaga tattttggat gttgcataag agtcctattt 60gccccagtta attcaacttt tgtctgcctg ttttgtggac tggctggctc tgttagaact 120ctgtccaaaa agtgcatgga atataacttg taaagcttcc cacaattgac aatatatatg 180catgtgttta aaccaaatcc agaaagctta aacaatagag ctgcataata gtatttatta 240aagaatcaca actgtaaaca tgagaataac ttaaggattc tagtttagtt ttttgtaatt 300gcaaattata tttttgctgc tgatatatta gaataatttt taaatgtcat cttgaaatag 360aaatatgtat tttaagcact cacgcaaagg taaatgagca cgttttaaat gtgtgtgtgc 420taattttttc cataagaatt gtaaacattg actgaacaaa tacctatatg gattggtaat 480gacttatgag caanctgctt ggccagacag ttacccaaac tttatatatn tnngaaggta 540tacactgnga aatctctggc taancgaatg cntccagggg taanngggtn tggntggant 600aaanaatgcc ctgcaaaaaa aaaaaaaaaa aagccttccg nggccttnaa nggaatcnnc 660angggnntnn ggccactggc cactggnaaa ngnaacgtct gga 703 169 609 DNA Homosapiens misc_feature (1)...(609) n = A,T,C or G 169 acgtccatcttccagctgct tgccagcaaa gatcagtctc tgctgatcag gaggaattcc 60 ttccttatcctggatcttgg cctttacatt ttctatcgta tccgagggtt caacctcgag 120 ggtgatggtcttaccagtca gggtcttcac gaagatttgc atcccacctc tgagacggag 180 caccaggtgcagggtggact ctttctggat gttgtagtca gacagggtgc gtccatcttc 240 cagctgtttcccagcaaaga tcaacctctg ctggtcagga gggatgcctt ccttgtcttg 300 gatctttgccttgacattct caatggtgtc actcggctcc acttcgagag tgatggtctt 360 accaagtcagggtcttcacg aagatctgca tcccacctct aagacggagc accaggtgca 420 gggtggactctttctggatg ttgtaatcag acanggtgcg ttcatctttc actgnttcca 480 caaaaaacaacctctgctgg canganggat ccttccttnc ttggactttg cctgacattc 540 tnatggngtactccgctccc ttcaaaggga tgncttacan tcanggnctt acnaaaattt 600 cntccnctt 609170 617 DNA Homo sapiens misc_feature (1)...(617) n = A,T,C or G 170acaaagaaca tgtagctata ggaaataata gtgtaaatag cagtatataa actggcccat 60gtaaaataca aaaatattca ctgaagtcag gttttctata aaacagtgtt tattagaggt 120attttactat gaatcaggca tataatctga atgtagaaac ttttagaaat attaacagca 180ttcagtcagt gccatgcact tgtgcttcca attatttttt taaagctgct ttgttttgac 240tcatgtgaaa tagttaaggc ctacattctt atacacatta tccatcttac aaggttaaca 300attttacact aaaacacagt ttaaattaaa aacgattttg aaaaattaca tctatattta 360atccctaaga agtgttttaa gctggtaatg cagctcgctg tagctctaag agaggggtta 420gtcaggaatc tgatcttgag ccataaangg tttcaggcta aacaaagaac aaatttaagt 480gacagaaaat attataattn caatatactc agttttttgg tataaaatac cctgctagca 540tgccactggc tatattgngg gcataatata aaatgncggg gggggggatg gancctccaa 600gncaaanttt ggaccca 617 171 621 DNA Homo sapiens misc_feature (1)...(621)n = A,T,C or G 171 acagtatggg ggttgtaaat tggcatggaa atttaaagcaggttcttgtt ggtgcacagc 60 acaaattagt tatatatggg gatggtagtt ttttcatcttcagttgtctc tgatgcagct 120 tatacgaaat aattgttgtt ctgttaactg aataccactctgtaattgca aaaaaaaaaa 180 aagttgcagc tgttttgttg acattctgaa tgcttctaagtaaatacaat tttttttatt 240 agtattgttg tccttttcat aggtctgaaa tttttcttcttgaggggaag ctagtctttt 300 gcttttgccc attttgaatc acatgaatta ttacagtgtttatcctttca tatagttagc 360 taataaaaag cttttgtcta cacaccctgc atatcataatgggggtaaag ttaagttgag 420 atagttttca tccataactg aacatccaaa atcttgatcagttaaaaaat ttcacataac 480 ccacttacat ttaccaactg gaagaataat caatctctcaagcatgggat tattagaatc 540 aacantttga aagctgtcct tgaaggctaa taaaaaagnttgtctaacct ttcatgaggn 600 cttnttntta ctnccttacn g 621 172 399 DNA Homosapiens 172 actcaaaatt acacatttgt ttaaataaat atccacacaa attctcagttacatcaagta 60 gctggtttat atttagatta tctcaagtag gggggaataa ccatgtgtaggaattcatag 120 aaaaataaac aatcagctga agaggtctaa gaaaatgctg acttttaaaatttcacttat 180 tttccttgaa gttttctacc cttcccatcg atgataaacc aagatcatgtaatggaaaat 240 ttcaaaccag ggctaaattc taaagtaaag cttcaattca agcccttcccccaagagaat 300 taattttcct gatttctctt tctctcacat ctaaggagaa cattttaggcagttaaattt 360 cagaacttca aggtttcatc agggtcacct ttatgtacc 399 173 616DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 173 actttgtggataagaaaatg gaggaacaca tctgatggag agtgggcatt tgacaacaat 60 ggaacaggtaacctgcatgt aaaatcaaaa tataagtgtc tttttaagag ctgaaagctg 120 ctgctggtcattcattaatg tgtcagacat ttaatcagga tgctggacct tcaaaataac 180 tgaaaaaagaaccaagaaaa ggcgtttttg ttttcaacaa actttactaa ataaccctgg 240 aaaggcaatgaacgatctga caatttaagc tctaatgatt taaagctcag ctagaagaaa 300 gtgaggcatgacatatactg tcaacggagg gtgaaggagg canatttctg gaaatgcaat 360 gatcccaccatttgcttcaa ngagaaacct gcanacatat tttcangtct tgntaagtna 420 caactgtntatttgtaatca atcatttngg aaaagtctgc tatgtaactt angncactgt 480 gcccccnaccaccgatgaaa aggaaaaacc cctgacacca ggaaaatcct tccatcctca 540 aanaaattaagngaccaacn tttaaagaaa aaaaatnanc ccncctctnt ttacaaatnt 600 ttcntccaaatnttcn 616 174 631 DNA Homo sapiens misc_feature (1)...(631) n = A,T,Cor G 174 ggtacgcggg gacacgcacg ccgggcgtgc cagtttataa agggagagagcaagcagcga 60 gtcttgaagc tctgtttggt gctttggatc catttccatc ggtccttacagccgctcgtc 120 agactccagc agccaagatg gtgaagcaga tcgagagcaa gactgcttttcaggaagcct 180 tggacgctgc aggtgataaa cttgtagtag ttgacttctc agccacgtggtgtgggcctt 240 gcaaaatgat caagcctttc tttcattccc tctctgaaaa gtattccaacgtgatattcc 300 ttgaagtaga tgtggatgac tgtcaggatg ttgcttcaaa agtgtgaagtcaaatgcatg 360 ccaacattcc agttttttaa gaaagggaca aaaggtgggt gaattttctggagccaataa 420 ggaaaagctt gaagccacca ttaatgaatt aatctaatca tgttttctgaaaacataacc 480 accattggct atttaaaact tgtaattttt ttaattttcc aaaatttaaatttgaanact 540 taaccccant tgccatntgn gtgacaataa aacattatgc tacccnttttaaaaaaaaaa 600 aaaaaaaaaa agtcctgccc ggcggccctc a 631 175 261 DNA Homosapiens 175 acgaacctac agttttaact gtggatattg ttacgtagcc taaggctcctgttttgcaca 60 gccaaattta aaactgttgg aatggatttt tctttaactg ccgtaatttaactttctggg 120 ttgcctttgt ttttggcgtg gctgacttac atcatgtgtt ggggaagggcctgcccagtt 180 gcactcaggt gacatcctcc agatagtgta gctgaggagg cacctacactcacctgcact 240 aacagagtgg ccgtcctaac c 261 176 616 DNA Homo sapiensmisc_feature (1)...(616) n = A,T,C or G 176 cgaggtactc tgccttttaggagatgaggt aagacatata catagatggc ttttactagc 60 caaggcaatg taaatggactaagattctca tgtgacttga ggttatctga tgaatttatt 120 ctcttcaaaa ccacctacctttagagggca tgtttaaccc ctctctttat ttaaggaggg 180 agagaaaaac acatgtaaccagaattcaga gtgggttact caacctaaga gaacatacgg 240 agttctcttt gggaaaacaacaagactaca gtgttcactt cgcaccatga agtggcactc 300 ctgttatggc tgtcagagtcctctcacttc ttatgaaagg atgcatctga ttctgaaatt 360 actgatatat tcgatcagttanggatgttt taaaaagtga aaacaaatgc cacacataca 420 ctttctagct ttcttgaaatcacccgacac attccaaaaa tagagaattc cctattactt 480 ttagagaaat ttccatatantcttggtnaa gaanccagtt gngcntattc caatttcagg 540 gtcttggttt ttgcccaaacccaagtgttt ccntntttta nggcttttca tggccgattt 600 naaaccttnt ttgtgg 616177 632 DNA Homo sapiens misc_feature (1)...(632) n = A,T,C or G 177cgaggtacag gtcagagtct tcttttcttt tctttttgag atggagtctt gctctgttgc 60cagactggag tgcagtggtg cgatctgggc tcactgcaat ctccacctcc cgggttcaag 120cgattctcct gcctcagcct cccgagtaac tgggactaca ggtgtgcgcc accaagccca 180gctcattttt gtatttttag tanagatggg gtttcacggt gttggctagg atggtctcga 240tctctggtca gaagtctttt ctgtaaatat ccttggtaaa gaagcaattt tagactgtag 300ctgttgcaaa tgctttaagg aagaagcaaa acaactgtca gtcttcctga aatgaaaaaa 360ctacaccagg gctgctatat caaagcaacc ccaaccagca cttcaatcat gatgcccaca 420gtggccccac tgagaaacca agaaaagttn cagatacaaa actgngatgc tcttgctatg 480gnaatattgc nggcngtanc caagttagaa accaaacaag cntanggccc cgttnttttt 540tggcgtgatt ttggcaanaa aaaaaactgg gngngtggtg ngggttccca ttgtaccccc 600aaaaaacttn gggatgggtt aaagcccnng gc 632 178 611 DNA Homo sapiensmisc_feature (1)...(611) n = A,T,C or G 178 actttntttt tttttttttttttttttttg ggatttagtt tttatttcat aatcataaac 60 ttaactctgc aatccagctaggcatgggag ggaacaagga aaacatggaa cccaaaggga 120 actgcagcga gagcacaaagattctaggat actgcgagca aatggggtgg aggggtgctc 180 tcctgagcta canaaggaatgatctggtgg ttaagataaa aaacaagtca aacttattcg 240 agttgtccac agtcagcaatggtgatcttc ttgctggtct tgccattcct ggacccaaag 300 cgctccatgg cctccacaatattcatgcct tctttcactt tgccaaacac cacatgcttg 360 ccatccaacc actcaatcttggcagtgcag atgaaaaact gggaaccatt tgtgttgggt 420 ccaacatttg ccatggacaaaatccangac ccgtatgctt taagatgaaa ttctcatttc 480 aaatttcttc ccataaatggacttgccnca tgccatnttg ggtgtgaagt nccnccttgc 540 ncataaccct ggaatatttttgaaacagaa ccttttacca atcntttttt catgttaaaa 600 acnaaaattt t 611 179 611DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 179 acctcaattttatcatttta gagtatttgt tagaatagga tctctccaaa atcaaacagg 60 atcaatctggtcacgtctaa tcctaagaca aaacactatg taaaattttc ctgtatctaa 120 atgttgccctctaggtaaat ctgtgatatt ttagagactt tcttttgtgg aaaaggtaat 180 ctgataaatgggaagagatc atcagacaag ttcacaaata accattattt ctgcagaatt 240 cagttgaagttggttttttg taaatgctta ttgggaattt ctaaagcact gacttggaga 300 ggccaagagcctccatcaat ccctgcttgg atagccactc ccgttactac tgctaggtca 360 gggtctacagatgtgttggg atcttttcca aagaactctt gaatgacttg acggatccga 420 ggaataccaatggagccccc aactaaaacc acctcatcaa tctcagtctt ttncaggtgg 480 ncttcttcaatctcctgaat gggacctcgg ccgcancacn ctanggcgaa ttccacacct 540 ggcggccgtactaatggatc caactcgnac caacttgggg aacatggcta gtnttcnngg 600 ggaaatgttt c611 180 621 DNA Homo sapiens misc_feature (1)...(621) n = A,T,C or G 180acccttaaac tggcaggaca tttttgaaat cacaaatttg cacataaaga atgtcacgaa 60cagccatgta tccatataca gcaatcaaat aaggaactta tgacctaaag caaaggtaaa 120ctttcttgaa acttaacatt ctataccaac taggcaacct ctgcccagga tgagagttgg 180atttttcaaa aacctctaat ttaatagtgc agcatttcgt tttccctgat ggcctgtgtt 240tcacagcagt ttttaaaaac tgcttgttca actatagctg cagcctatat cccagctatg 300gaaaaaaaag taaatcttag ttcaattttt gccagttgtt tctgtattta aatttaaaaa 360aaaacacact tccgctgggc aggtttagag ggttattatc aagtctgtgc ataactaaaa 420gttcaaagca aattcaattt tgcttaangg aacattgnna aagnacaatt cttggnanta 480catgcctcgt tgatccattt naancatana aaattcaccc ttgtgtactg gttcaagaaa 540aaaaccgatt tgacagttaa acatnttaaa anccccaacc tntgaagttc aaccaaactg 600ganttttgtt cctcgcccga c 621 181 606 DNA Homo sapiens misc_feature(1)...(606) n = A,T,C or G 181 cgaggtacag accagagaca aagcaagagaagaagcagag actgttggcc cgggccgaga 60 agaaggctgc tggcaaaggg gacgtcccaacgaanagacc acctgtcctt cgagcaggag 120 ttaacaccgt caccaccttg gtggagaacaagaaagctca nctggtggtg attgcacacg 180 acgtggatcc catcgagctg gttgtcttcttgcctgccct gtgtcgtaaa atgggggccc 240 cttactgcat tatcaangga aaggcaagactgggacgtct agtccacaag gaagacctgc 300 accactgtcg ccttcacaca ggtgaactcggaagacaaag gcgctttggc taaactggtg 360 gaagctatca ggaccaatta caatgacngatacnatgaga tccccctcct ggggtggcaa 420 tgtcctgggt ctaaatctgt ggcttgtatngccaacttcn aaangcaaag cttaaaaact 480 tgcncttaac tngggtnaat gtactncccggcggccgttg aanggcaatt caacacattg 540 cggccgtcta atggntcanc ttggnccaacttgggnaana tggnaaannn ttcttgggna 600 atttnn 606 182 610 DNA Homo sapiensmisc_feature (1)...(610) n = A,T,C or G 182 ggtactcata aaaaaagtcttaccccaaaa ttgcaaacaa atacattaaa agattagaag 60 aggtgataga aagcaccagacattaaacaa aataaaaata ataaaataaa ttcaactcaa 120 aaggtcccca ttcagcaaatactttgtaaa gtatggcctg tatgtaaata gtgctaaatc 180 aaggactttt tagcagaaaattgctcggtt cttttatcta aggcttgaat ttgtaaagtg 240 aaggcataaa agttaccaaacattaagtaa ctcttaaaat ggcacacagg ttttaaagct 300 attggttttt ccttcctaactctctgaatt tttcccatgg cctttgtaga tcaactattt 360 caaacgtatt ttacaccagcaactctcaac atacttgtct ttcagatatg tcatcagtca 420 tgtctaacag gccaatagccaaataacnga tttaaaacaa tncttaacta gctagcagga 480 cattactttg gatctgcttactgcaactga ctatttgtaa gcttaaaatc antttaatcc 540 tgatacagaa acctcatctgcncatacntt actttggcct tcaaccttta aaaatactta 600 atcccccgnc 610 183 608DNA Homo sapiens misc_feature (1)...(608) n = A,T,C or G 183 cgaggtactttttttttttt tttttttttt tttttatttt tttttttttt tttttttttt 60 tttttttgggagncagctnt ttaattaggn tcttaaaaca tttaaaacnc caatttgnga 120 ggataaattccattcgtcan ancaaacnca aatcgcaggt anccctggan ctgaggaata 180 nctttgatttttggnaaaat ttgngagtcc acagctttnt gatcaatntt gcnctgctcc 240 gnaatctcatatttctnttt ttctgngncg aaaatctcac cttcctggng tntgggcttc 300 cgcagcttnttntttttgaa gtaagcatca ataaaangtt ttgggatttt tacattgctg 360 aaatccattttgggtgaagg ggcaatgaca aatttntngn gtnttctttt taaaagaacc 420 tcattgggggccnaaggncc cncccaaatt ataaacccct ttccccctgg tttangnaaa 480 cccccctttgccctgngggg nccangagga taaanaaagg ccccggggaa gctggcccca 540 ntttttcccgccgncgaagg gttttgccgg ctaaaanttt tngggcattt nnngggnaat 600 tttggctt 608184 622 DNA Homo sapiens misc_feature (1)...(622) n = A,T,C or G 184acagccctga tgcaaagttt cagagcatga ccagcaagtg gccagctgtg tgggtcaaga 60tcagctccag ctgggtctgc ctcctgcttt acgtctggac ccttgtggct ccacttgtcc 120tcaccagtcg ggacttcagc tgaacctctg agtgccaagg acaccactgg aactcacaaa 180ggtctccttc accgaaaacc catatacctt ttaagtttgt ttcaactaaa atattaagtg 240aatgctttgc aagtttgact gtatgcaggt ttatatcaag aaggtgagat tgaataatgc 300ttgatgcaga atcgaaactt ctcatttatc tgnatattat gtttacttct aaggatatag 360cacaaaggga acattttttg tttaaagtga actacagctg tgctgtgaag agagttcttt 420ataaagcctg taggtctttt aactttggtt aaaatgtaag ataggaaaat gttggatatt 480tgaggcntgc ctaatatatt tatattggag natcctttna aagccaaaaa aaaaaaaaaa 540aaaaaaaagt nccttggccg gaccncccta aggggaattc cacncactgg gggccgtntt 600atggatccaa ctcgnaccaa ct 622 185 614 DNA Homo sapiens misc_feature(1)...(614) n = A,T,C or G 185 acgcggggac agtcccaccc tcacacgattctttaccttt cacttcatct tgcccttcat 60 tattgcagcc ctagcagcac tccacctcctattcttgcac gaaacgggat caaacaaccc 120 cctaggaatc acctcccatt ccgataaaatcaccttccac ccttactaca caatcaaaga 180 cgccctcggc ttacttctct tccttctctccttaatgaca ttaacactat tctcaccaga 240 cctcctaggc gacccagaca attataccctagccaacccc ttaaacaccc ctccccacat 300 caagcccgaa tgatatttcc tattcgcctacacaattctt cgatccgtcc taacaaacta 360 agaggcgtcc ttgccctatt actatccatcctcatcctag caataatccc atccttcata 420 tatcccaaca acaaagcata atatttcgnccactaagcca atactttatt gattctagcc 480 ggagacctct nantntaacc tggatcggaggaaaccagta gctacccttt accaatantg 540 ganaagaaga tcgnaccttg gcgggacaccttangggaat tcaaccactg gnggcggtat 600 atgggacccn ccng 614 186 627 DNAHomo sapiens misc_feature (1)...(627) n = A,T,C or G 186 ggtactgattttaaaaacta ataacttaaa actgccacac gcaaaaaaga aaaccaaagt 60 ggtccacaaaacattctcct ttccttctga aggttttacg atgcattgtt atcattaacc 120 agtcttttactactaaactt aaatggccaa ttgaaacaaa cagttctgag accgttcttc 180 caccactgattaagagtggg gtggcaggta ttagggataa cattcattta gccttctgag 240 ctttctgggcagacttggtg accttgccag ctccagcagc cttcttgtcc actgctttga 300 tgacacccaccgcaactgtc tgtctcatat cacgaacagc aaagcgaccc aaaggnggat 360 agtctgagaagctctnaaca cacatgggct tgccaggaac catatnaaca atggcagcat 420 caccagacttnaagaattta agggcatctt ccacttttta ccaaaacngn gaacaatctt 480 tttcnttacttaacnaacnt gcttccatgg gagccgggng naatccaatc aagggcataa 540 cccgggccttatttggcnng atgggtcang gnaatancct gaccaggaaa cccctgnttc 600 cttggggggaantttgttgn nccccac 627 187 256 DNA Homo sapiens misc_feature (1)...(256)n = A,T,C or G 187 ggaccttttt tttttttttt tttttttttt ggaaaagaaaggccttacat atttattact 60 gaatccagcc aaccaacgtg ttcataacag attcagagaggaaaacacgt cgaaatctcc 120 anatagtggt gacattttca gcttgatatg gtaacatgatcgtgaccttc anacagcata 180 aatatgtgtg ccatctcatg tgcaattcct tatanacccagcttggttct tctccaatgt 240 ctccttttgg agttgt 256 188 523 DNA Homo sapiensmisc_feature (1)...(523) n = A,T,C or G 188 ggtaccacct acacccaacaagtcaatgag ggacttcttt ttaatttggt aggattttga 60 ctggttttgc aacaataggtctattattag agtcacctat gacaaaaaat aggggttacc 120 tagataatgc caaagtcagcatttgtcctg ggttcccttg tgtgatctgt ttggactatg 180 ttttcttttc ttctcccacttgctcagcag cttgggcttc cattctagct cttttaccaa 240 gatttttgtg tgaccatgttgacttcattt ggattgccct ctttcaattt ccttgtgaaa 300 acacccttaa ctttctctttacccttagct gaaatgttta cataacttct ggtgatatct 360 tttcatgatt ttatatctcttaaaatggtg atggatgtga cacctcataa aagtgagctt 420 tgaactgtag ataactcttaaagaaaatgt cattttanac aattaaaata tttgtgctca 480 aaaaaaaaaa aaaaaaaaaagtcctgcccg gcggccgtcn aan 523 189 622 DNA Homo sapiens misc_feature(1)...(622) n = A,T,C or G 189 acaatttaat ttttctgctt gcccaagaaacaaagcttct gtggaaccat ggaagaagat 60 gaaaatgaga ctggcaaaga acaaatgctgaatctgaaga agaggacaac tttgggcaaa 120 taatctgcat acttttaatt gggaataagatggaaaatat gaatgctaaa tcaaattttt 180 taaaaaatac accacacgat acaactcaatacaggagtat ttcttctcaa attcttctag 240 caccatcaac attcttcaag tatctgaaatactattaatt aagcaccttt gtattatgaa 300 caaaacaaaa caaggacctc agttcatctctgtctaggtc agcacctaac aatgtggatc 360 acactcatgg gaaagtgttt tgaggtagtttaaacctttt ggaaggttgg gttttaaact 420 tccctctgtg gaagatatca aaagccccaagtggtgccaa atggttatgg ttttattttt 480 caattttaat ttgggtttct tccaaaggtgacatttccat acaaggggaa gggggtggaa 540 aaaaaatcaa attttggggg accagggaggataatnaact gtttgcaatg cttgacaacc 600 tttttttttt gnccaantaa ca 622 190628 DNA Homo sapiens misc_feature (1)...(628) n = A,T,C or G 190accactaata gggtgtatct cagaaactga attgaaataa gggaaaatag gattttctgt 60cctggttttt gaagattgtt cttgattccc ttgattccca ggagagattc tctgacattc 120acgtgtcagc cactttggca cggaagcctt acagtgtggg gaaccaaaac ttcgtgtctc 180ctctttcccc gatgccatca gcatagactt gacttcctta aaccgagagt tttgatgtgg 240ccttggcaac cctaaaatca gctgtgttag gtaacaaaac tcaggctttc tgttgatgac 300atcgagatgg tgtcacttaa aagagccaag attcctgttt tcagtttgtg gattcatcct 360gctggtttta ctttagtccc tccatgtcaa agtgggcctg agaaaagctc atacatgcct 420catgtgaagt gtccaccccc tctgaaaatc tttcttgttc aaaacancna cgacatatct 480tggtaacttt tacggtgact tttggangag gggagtttgg aaattgtaaa atgttatana 540tggtgcctat ttcctgctga angaaatgtt ttaaaaagnn tntntaancn taatcnaatg 600gttggggggn gaccttctac cnaanntn 628 191 474 DNA Homo sapiens misc_feature(1)...(474) n = A,T,C or G 191 ggtacagccc tcaatctgtt cttcaagctcaagaacttca agacagctgc cacctttgct 60 cggcgcctac tagaactcgg gcccaagcctgaggtggccc aacagacccg aaaaatcctg 120 tctgcctgtg agaagaatcc cacagatgcctaccagctca attatgacat gcacaacccc 180 tttgacattt gtgctgcatc atatcggcccatctaccgtg gaaagccagt agaaaagtgt 240 ccactcagtg gggcctgcta ttcccctgagttcaaaggtc aaatctgcag ggtcaccaca 300 gtgacagaga ttggcaaaga tgtgattggtttaaggatca agtcctctgc agtttcgcta 360 aagccccctt tgtgtgcatg ggtcaagtcaccatatgttc cccccaaaaa atgtgtctat 420 atctccttct aacaacacct tcccctgcactactcttcaa atctngctct ntgt 474 192 234 DNA Homo sapiens 192 acgcgggggttggtgagtgg gctcctaccg accgaggttt aggcagcgcg gggagctttg 60 cgggttgccatttgtaactc cggatcctaa aattcctgtc ctgttctctg tctcttctag 120 gttgggggccgtcccgctcc taaggcagga agatggtggc cgcaaagaag acgaaaaagt 180 cgctggagtcgatcaactct aggctccaac tcgttatgaa aagtgggaag tacc 234 193 367 DNA Homosapiens 193 ggtaccaata ccaccaattt tgtagacatc ctggagaggc aggcgcaagggcttgtcagt 60 tggacgagtt ggtggtagga tgcagtccag agcctcaagc agcgtggttccactggcatt 120 gccatcctta cgggtgactt tccatccctt gaaccaaggc atgttagcacttggctccag 180 catgttgtca ccattccaac cagaaattgg cacaaatgct actgtgtcggggttgtagcc 240 aattttctta atgtaagtgc tgacttcctt aacaatttcc tcatatctcttctggctgta 300 gggtggctca gtggaatcca ttttgttaac accgacaatt agttgtttcacacccagtgt 360 cccgcgt 367 194 613 DNA Homo sapiens misc_feature(1)...(613) n = A,T,C or G 194 ggtactcttg gtttgtcaat gggactttccagcaatccac ccaagagctc tttatcccca 60 acatcactgt gaataatagt ggatcctatacgtgccaagc ccataactca gacactggcc 120 tcaataggac cacagtcacg acgatcacagtctatgcaga gccacccaaa cccttcatca 180 ccagcaacaa ctccaacccc gtggaggatgaggatgctgt agccttaacc tgtgaacctg 240 agattcagaa cacaacctac ctgtggtgggtaaataatca gagcctccgg tcagtcccag 300 gctgcagctg tccaatgaca acaggaccctcactctactc antgtcacaa ggaatgatgt 360 aggaccctat gagtgtggaa tccanaacgaattaagtgtt gccacagcga cccagtcatt 420 ctgaatgtcc tctatgncca gacgaacccccatttcccct cataccctan taccgtcaag 480 ggtgaacctt agctttctgc atgcagctttaaccactgcc agtttcttgn tgatgatgga 540 catcacacca cacaagactn ttatttcacatactgagaan aaagcgactt ntactgcagg 600 cataactanc ngg 613 195 613 DNA Homosapiens misc_feature (1)...(613) n = A,T,C or G 195 acgcgggcgccagagtccct gaactctcgc tttcttttta atcccctgca tcggatcacc 60 ggcgtgccccaccatgtcag acgcagccgt agacaccagc tccgaaatca ccaccaagga 120 cttaaaggagaagaaggtga tggtgaggaa gaggatggag atgaagatga ggaagctgag 180 tcagctacgggcaagcgggc agctgaagat gatgaggatg acgatgtcga taccaagaag 240 cagaagaccgacgaggatga ctagacagca aaaaaggaaa agttaaacta aaaaaaaaaa 300 aggccgccgtgacctattca cccttcactt tccgtctnaa aatctaaacg tggtcacctt 360 caataaaaaggccccccgcc cccngggcag tgccccccca aaataaacgc gctttcacca 420 ccaaccaaacatgaaaattt tccacaaggg anggaaaaaa aaccaaacnt ccaaggcctn 480 tttttttttaaaatactngg ccgcgaccac cctanggcga attccanacc tggcggccgt 540 nttatggatccnactcggac caacttgggn aatatggcat antggttctt ggngaaatgt 600 atccctccattcn 613 196 296 DNA Homo sapiens misc_feature (1)...(296) n = A,T,C or G196 gcggnggcnn ggccgacgnn ctcatcaatg ttgttcggtc agcccttccc taattacacc 60tatccnctac acatacatgc acatagacac acncntgaac ncactgaana tatttccttc 120aggtgtgtgt aaaatatgct gcttggattg aaattcannt gggattgatt agncaagtan 180cttganacct cacagtaatc ttcacacttn nccttacaca cctatgcagg catgttggga 240gcangttaca atgttacttc agcccacagt ttatttctat acttgagttc ttaagt 296 197222 DNA Homo sapiens 197 acatggagga gaatgaccag ctcaagaagg gagctgctgttgacggaggc aagttggatg 60 tcgggaatgc tgaggtgaag ttggaggaag agaacaggagcctgaaggct gacctgcaga 120 agctaaagga cgagctggcc agcactaagc aaaaactagagaaagctgaa aaccaggttc 180 tggccatgcg gaagcagtct gagggcctca ccaaggagta cc222 198 539 DNA Homo sapiens 198 cgaggtacta catatttcag cactaaggcggttgcttcac tttatatcta tataaaaaaa 60 gtggtaaaaa tcttttcctt ttgtgcagttgaacccatcc tacattcaga ttctctcaag 120 cactaataaa atacttattt ggttgaggaagatttaaggc aagttcgggc ccttccaaag 180 gcactgtgag actccccccc cactccccgttattgctaca tgtctttata ctcgagtatg 240 tcacagtaga actggtggaa taagcaaacacttttttgct agtttataaa gttggaatta 300 gaaaagcatg ccacatttca gcctgattgcaaagtatgtg gtcatttttt tctttgaagt 360 tggatgggct acaaccttta tacattctaagaaaactcat aggatgttcc tcaaactact 420 tccacagcat caagatcgat ttctgtcaagaaatcatgca atctttcaaa atttacgtaa 480 acaaggaaag aaattaatga aataaatattacatacaatc tcttaaatta agaatttgt 539 199 626 DNA Homo sapiensmisc_feature (1)...(626) n = A,T,C or G 199 cgaggtacaa gatgtccaaatattgcgaag atctatttgg ggatctcctg ttgaaacaag 60 cacttgaatc acatccacttgaaccaggca gggctttgcc atcccccaat gacctcaaaa 120 gaaaaatact cataaaaaacaagcggctga aacctgaagt tgaaaaaaaa cagctggaag 180 ctttgagaag catgatggaagctggagaat ctgcctcccc agcaaacatc ttagaggacg 240 ataatgaaga ggagatcgaaagtgctgacc aagaggagga agctcacccc gaattcaaat 300 ttggaaatga actttctgctgatgacttgg gtcacaagga agctgttgca aatagcgtca 360 agaaggcttc agatgaccttgaacatgaaa acaacaaaaa gggcctggtc actgtagaag 420 atgagcaggc gtggatggcatcttataaat atgtaggtgc tccactaata tccatncata 480 tttgtccaca atgatcaactacgcccacct gtaaaggttc aaggttncat gtggcagaag 540 aaccncatat tcattatacatggcttcttt tatgaatant cggccttggt tcttgaancc 600 cttgcaatga atttgnaattntacca 626 200 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,Cor G 200 actcataaaa aaagtcttac cccaaaattg caaacaaata cattaaaagattagaagagg 60 tgacagaaag caccagacat taaacaaaat aaaaataata aaataaattcaactcaaaag 120 gtccccattc agcaaatact ttgtaaagta tggcctgtat gtaaatagtgctaaatcaag 180 gactttttag cagaaaattg ctcggttctt ttatctaagg cttgaatttgtaaagtgaag 240 gcataaaagt taccaaacat taagtaactc ttaaaatggc acacaggttttaaagctatt 300 ggtttttcct tcctaactct ctgaattttt cccatggcct ttgtagatcaactatttcaa 360 acgtatttta caccagcaac tctcaacata cttgtctttc agatatgtcatcagtcatgt 420 ctaacaggca aatagcanaa taacagattt aaaacaatcc ttaactanctagcaggacat 480 ttactttgga ttctgcataa ctgcaaactg acatatttgt aaagctaaaaatcagtttaa 540 tcntgattac agaaactcta tcatgctcat tacttaacta ttgnccttcaatcgctattn 600 aaattcactt aatccaat 618 201 627 DNA Homo sapiensmisc_feature (1)...(627) n = A,T,C or G 201 ggtactaggc acaatagaacatacagaaaa cattgtccct gctcttgagg agcttacatt 60 ctaaaagaaa aaatacaccttttttaaaat ggcatttttg tttggtgttt tctgcaaagt 120 acgcggggct ttttctttttgaggaagacg cggtcgtaag ggctgaggat ttttggtccg 180 cacgctcctg ctcctgactcaccgctgttc gctctcgccg aggaacaagt cggtcaggaa 240 gcccgcncgc aacagccatggcttttaagg ataccggaaa aacacccgtg gagtcggagg 300 tggcaattca ccgaattcgaatcaccctaa caagccgcan cgtaaaatcc ttggaaaagg 360 tgtgtgctga cttgataagaggcncanaag aaaagaatct canagtgaaa ggaccaagtt 420 ngaatgccta ccaagactttgagaatnact acgaganaaa ctccttgtgg tgaaggtcta 480 agacgtgggn tngnttccagatgagaattc acaagcgact tattgacttc acaagtcctt 540 ntgagattgt tangctgattacttccttna ntatganccn ngaatttaag ngggangtna 600 ccntncagan gnttagttnactatttt 627 202 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,Cor G 202 actgcttaac gaaacactat cagcttgttt taaatggatc ttttaaatatcaactgtagc 60 ctggttggct aattctttct aatcttcccc attactttcg cctagatttcccatagatca 120 acaggcatag taaaatgcct catcagaaca cacttctcca cacaattcaaaaagggagct 180 cctgtgggct caaagcaacc atcagtccag caatgcccat gatttatctgaaactgcttc 240 ccaagagaca ggagtgcaga tctgagtagc tgtgctgcca atacagataggtttagcact 300 agatatttag tgattgtggc aaggaagaat cggtgatgat gggggtggtgggtgaaggaa 360 gggccagggg atctgaagga tcttcagttg ccttctcctg cttcttcatcctgctggtcg 420 ctcgtccana gggtgaggtt gtctcgcagc aactgcatga tcagcgtggagtccttatag 480 gaatcctcgt ttagtgtgtc cagctcagct atggcatcat cgaaggcttgtttggctaaa 540 agcangcttg ctcangtgca ttctggatct catagtagaa caccggagaantganggcca 600 ggcccaaccg gatnggatgc 620 203 577 DNA Homo sapiensmisc_feature (1)...(577) n = A,T,C or G 203 ggtacttttt tttttttttttttttttttt tttttttttt tttttttttt tgaaaaagtc 60 atggaggcca tggggttggcttgaaaccag ctttgggggg ttcgattcct tccttttttg 120 tctaaatttt atgtatacgggttcttcnaa tgtgtggtag ggtggggggc atccatatag 180 tcactccagg tttatggagggttcttctac tattaggact tttcgcttcn aagcgaaggc 240 ttctcaaatc atgaaaattattaatattac tgctgttaga naaatgaatg ancctacaga 300 tgataggatg tttcatgtggggtatgcatc ggggtantcc gagtaacgtc ggggcattcc 360 ggataggccn agaaagtgttntgggaanaa agttagattt accccgatga atatgatagt 420 gaaatggatt ttggcgtaggtttggtctag ggtgtancct gagaataggg gaaatccgtg 480 aatgaaacct cctatgatggcaaatacact cctattgnta ggacataatg ngaagtgagc 540 tacaaccgta atacctgcccnggcnggccc ttannan 577 204 629 DNA Homo sapiens misc_feature (1)...(629)n = A,T,C or G 204 cgaggtactt gttttttttt ttttttttga gacggagtctcagtctgtca cccaggctag 60 agtgcagtgg cacgacatcg gctcactgca acctccgcctcccgggttca agtgattctc 120 ctgcctcaac ctcccgagta gctgggacta caggcatgtgccaccacgcc tgactaattt 180 ttgtattttt agtanagatg ggatttcatt atgttggccagctggtcttg aacttctgag 240 ctcaggtgat ccacccgcct tagcctncca gagtgctaggataacaggca tgagccgtcg 300 cgcctggcca aaatagcata atgttttaag aaagtttacgaatttgtctt gggccacatt 360 naaaaccatc atgggccaag ggttggacaa gctagccttaggtcatgtca gaatgcaatt 420 taacaggaat ttcaagcnaa acttacaaaa aattaaatccacaaaaaaaa tatcatttgg 480 taaatgcact gnctacacac tttactncta agtccattcaaccatgacga ccctttacat 540 aaaaattagg gcattctccc aagttctaaa gatgatttctaaaacattac caangnctaa 600 agtctaattc ccacaaanca ttttttttn 629 205 424DNA Homo sapiens misc_feature (1)...(424) n = A,T,C or G 205 ggtacaaatgcttttatatt cagcccctgt aaagccatca gatgtttgaa agtttttaaa 60 cacgaaccaaagggtttaat tttaagaact tagctaggaa tgggtgaaat cctacccaat 120 taatagagttctgcaaatta gtaacaaagt gtaaaatgaa aggaagggtc ccttggagat 180 gtgaaattcttctattgaga gtcctgtctt ctttattcaa gaagtttgta gccattttca 240 gaattcactcaagaaccaac ttcttaattt agatatcagc gaacaagtca tggcaaaaaa 300 tacacaaagagaaacaccac cacatcgaaa aggatgaaaa gccagaggtc caaccagtan 360 gagtgtttgggaagcccatt tgccccagac tgaggcctca catcgaagtt ctgcctcccc 420 gcgt 424 206633 DNA Homo sapiens misc_feature (1)...(633) n = A,T,C or G 206ggtaccaatg gtgcctcctg gaatcaagta tctttacctt aggaataacc agattgacca 60tattgatgaa aaggcctttg agaatgtaac tgatctgcag tggctcattc tagatcacaa 120ccttctagaa aactccaaga taaaagggag agttttctct aaattgaaac aactgaagaa 180gctgcatata aaccacaaca acctgacaga gtctgtgggc ccacttccca aatctctgga 240ggatctgcag cttactcata acaagatcac aaagctgggc tcttttgaag gattggtaaa 300cctgaccttc atccatctcc agcacaatcg gctgaaagag gatgctgttt cagctgcttt 360taaaggtctt aaatcactcg aataccttga cttgagcttc aatcagatag ccagactgcc 420ttctggtctc cctgtctctc ttctaactct ctacttagac aacaataaga tcagcaacat 480ccctgatgaa gtatttcaag cgtttaatgc tttgcagtat ctgcgtttat ctcacaacga 540actggctgat agtggaatac ctggaaattc tttcaatggn gccatcctgg gtgaacctgg 600acttgcctat accagcntaa aacataccac cgg 633 207 623 DNA Homo sapiensmisc_feature (1)...(623) n = A,T,C or G 207 ggtacttttt tttttttttttttttttttt ttagaaacta tggctcttta ttttcatgtg 60 gataattcaa acaaagtcattagtagtctt tgttcaattt ttttttaaaa aacaaaaaaa 120 ccctcaaata aaaaatcttgggcttaaaag aactctatca caggagcctg gttggaggat 180 tcctagtttt atacatgagaaatagaatgc agatttctct gaagagtgtt taaagaagga 240 atggtagttg agggggcttatttcccaggc tcaaagtgat ttaggggtgg tgtcacagtg 300 ctaggtatag ggtgatggacagtgatcact gccgagggcc ttggaacgga tcttgctgtc 360 acacaatgca ggtaacagagagtgggacaa caaaaagtaa tcaaggcgcc aaccaacatt 420 cttggatcga gcattcatatataagtccaa aaggtgtang cataaggtgt gttggggtan 480 aagtgcctaa agctgcaaccagtggcacan cctgcagtaa ttccccgaac cttggccttt 540 tggggcgtga anccnccattcttttggtnc cctnggggtg cnaaggcaat ttttnatgtg 600 cccattgagg gttcaaacacaca 623 208 620 DNA Homo sapiens misc_feature (1)...(620) n = A,T,C or G208 acgatgtcta gtgatgagtt tgctaataca atgccagtca ggccacctac ggtgaaaaga 60aagatgaatc ctagggctca gagcactgca gcagatcatt tcatattgct tccgtggagt 120gtggcgagtc agctaaatac tttgacgccg gtggggatag cgatgattat ggtagcggag 180gtgaaatatg ccccgcgtac ttgctttgaa agattaccta ctattttatg ataaaatgta 240gttgtctcca gagcttaaat ataatttgta aagcacttgg tttaaatttc tctctaccta 300taaacagttt agcattaagg gtttctatta atgacacaga attattggcc aagtgtaatt 360tcttaaaatt tagcattact ttaaatagcc agcatgtaat acaagtaact acactacctc 420atatctacat gattttcaag ttgtaatgca gatggacaga taaaaaagat ttacgttgnc 480ttttggccat aagtgggaaa agttttctgn atattgcata gcattacaca tttatgccta 540ttttacatta acttctaaag aagtttttct aagaaaangg ttcaggcaat attttttgag 600gctgccgaan aaaaatgant 620 209 624 DNA Homo sapiens misc_feature(1)...(624) n = A,T,C or G 209 ggtactggta caaaaacagg cacataaaccaatgaaacag aatagaaagc ccagaaataa 60 tgcttcaccc ccacaaccat ctgatcttcaacaaaataaa caaaaacgag ccatggggaa 120 aggactccct attcaataaa tggtgctgggataactagtt aaccatatgc agaagattaa 180 agctggaccc cttccttaca aaataaggagctggacccct tatacaaaaa tcaactcaag 240 atggattaaa gccttaaatg tgaaactataaaaccctgga agacaacata ggcgattcca 300 ttctagacat cagaactggc aaagatttcatgaggaagac accaaaagca attgcaacaa 360 aagcaaaaat tgacaactgg gatataattaagtttaagag cttctgcaca gcaaaagaga 420 gactatcagc agagtaaaca gaccacctacagaatgggag aaaatatttg caaactatgc 480 atgtgacaaa ggtctaatat ctagcatctataagtactta aacaaatttc aacagaaaac 540 caacacccca ttaaaaagtg ggcaaggacatgaacaaatg cctttcaaaa gaagacatct 600 gcttntacag tttntgaaac aaag 624 210504 DNA Homo sapiens 210 acgcggggca gctagcagat gctttaggac ctagtatctgcatgctgaag actcatgtag 60 atattttgaa tgattttact ctggatgtga tgaaggagttgataactctg gcaaaatgcc 120 atgagttctt gatatttgaa gaccggaagt ttgcagatataggaaacaca gtgaaaaagc 180 agtatgaagg aggtatcttt aaaatagctt cctgggcagatctagtaaat gctcacgtgg 240 tgccaggctc aggagttgtg aaaggcctgc aagaagtgggcctgcctttg catcgggggt 300 gcctccttat tgcggaaatg agctccaccg gctccctggccactggggac tacactagag 360 cagcggttag aatggctgag gagcactctg aatttgttgttggttttatt tctggctccc 420 gagtaagcat gaaaccagaa tttcttcact tgactccaggagttcagttg gaagcaggag 480 gagataatct tggccaacag tacc 504 211 619 DNAHomo sapiens misc_feature (1)...(619) n = A,T,C or G 211 accatgaaatatccagaaca tacttatatg taaagtatta tttatttgaa tccacaaaaa 60 acaacaaataatttttaaat ataaggattt tcctagatat tgcacgggag aatatacaaa 120 tagcaaaattgaggccaagg gccaagagaa tatccgaact ttaatttcag gaattgaatg 180 ggtttgctagaatgtgatat ttgaagcatc acataaaaat gatgggacaa taaattttgc 240 cataaagtcaaatttagctg gaaatcctgg atttttttct gttaaatctg gcaaccctag 300 tctgctagccaggatccaca agtccttgtt ccactgtgcc ttggtttctc ctttatttct 360 aagtggaaaaagtattagcc accatcttac ctcacagtga tgttgtgagg acatgtggaa 420 gcactttaagttttttcatc ataacataaa ttattttcaa gtgtaactta ttaacctatt 480 tattatttatgnatttattt aagcatcaaa tatttgtgca agaatttgga aaaatagaag 540 atgaatcattgattgaatag tattaagatg tatagtaaat tatttatttt ananattaaa 600 ngangtttattaganaaan 619 212 479 DNA Homo sapiens 212 cgaggtacaa agcagcaactgcaatactca aggttaaaac attagaaaag catttgtgtg 60 acaggtatat tacagtattatcaaaatatt acattttcag acttacttag cagataatca 120 tccaccagag cttaaatctttaaattattt ccatagtctt aaaaaatatg taatgtcaga 180 atgcatataa aaagaatgtaaaaggaaacc taaaatacaa atggaataat gtaacaaata 240 aatatttgat ttcagtaactgttaataatc agctcaacac caccattctc tctaaactca 300 atttaattct tataggaataatgaactgtc aaatgccatg gcataattat ttatttccaa 360 gctatcatca atgattagaactaaaaaaat tttggcataa aaaaatcaca attcagcata 420 aataaagcta tttttagcttcaacactagc tagcatctct aagaattgtt gaaataagt 479 213 487 DNA Homo sapiens213 actgtttact gcctgggcac tatactttct atgcagatct cctttgtggg tttccagcct 60gtcctttcat cagagcacat ggcagccttt ggggtctttg gtctctgcca gatccatgcc 120tttgtggatt acctgcgcag caagttgaat ccacaacaat ttgaagttct tttccggagc 180gtcatctctc tggtaggctt tgtccttctc accgtgggag ctctcctcat gctgacagga 240aaaatatctc cctggacggg gcgtttctac tcactgctgg atccctctta tgctaagaac 300aacatcccca tcattgcttc tgtgtctgag catcagccca caacctggtc ctcatactat 360tttgacctgc agctcctcgt cttcatgttt ccagttggcc tctattactg ctttagcaac 420ctgtctgatg cccggatttt tatcatcatg tatggtgtga ccagcatgta cctcggccgc 480gacacgc 487 214 393 DNA Homo sapiens 214 cgaggtacaa tatgctgcagcataatttgt caggccaacc ttcacaccat attttggcag 60 ttcgtgtgca tacgctgcgcagactatcat atccccctct atacgggcat aagcaatctg 120 acaaatgata tctctgtttgtcacacgaac tatcatcctg tatttgggtg tgttgtattt 180 atttttatct tgtatcaccaagcgtttccg agcataataa tcagttttac cctctcgtcg 240 tcttctaaat ttcacttggtatctcttaaa gtaggcctta ttcttaacaa ctttaacaaa 300 ccccatcctg cggaacagagaccggcgtcc gctgctcgac agagacctgc aggcccagcg 360 gcgctagggg gtgggaaaagggccaccccc cgt 393 215 615 DNA Homo sapiens misc_feature (1)...(615) n =A,T,C or G 215 ggtacagtaa caagtgttgg cattatcagt tgaactgtaa atacaaaatgcttcttccaa 60 ttagtctcta tgatgattaa gtttctaaaa tttatctgaa caccattcagaaacttgttt 120 tggggaattt gatagttatt gatgtgcatc tgttaaactg atgacagacataactcatca 180 ttccccagaa accttttttg attacagtat ctaacatttt gcctcctcttttttggtttt 240 gctggttata aaggtttgga ttggagaggg ctcactggat cccaatccttggagctggat 300 cattggattc aaatcataat gtggatagga tagggaggat gaattaccaggattcatgga 360 gcgggatcag attaccagga acataggagt ggattcctgc ccaaccaaacccgcattcgt 420 gtggattttt ttattcaact taattggcta ttccaaagat ttttttttcctatttttgac 480 gaatggagcc cttaagatgc acgatggaat tgggtttgcg tttttggtaaaaggaccaaa 540 ccaggcctgg agataacgct ggagcaatct cntggaagga ttagccccaattgatgggaa 600 catttaangg ggaag 615 216 322 DNA Homo sapiensmisc_feature (1)...(322) n = A,T,C or G 216 ggtacttttt tttttttttttttttttttt ttttttggag ttgtaggcaa atgtttaatt 60 aattctgctc atatgcacatctgaaagcat gagacacact ccacagacag cacgcactgg 120 ggctggtggg gcanatgggcactcgccgat taggtattaa tgtcaataat acgtgcataa 180 agtgctgata aaataacttaagtgttacaa aaagagacag tccacggtgg ctgcaggcac 240 atgcaggcgg gactgggtcaaacactccag ggctgcacat gttccagctg gcctgagtcc 300 gacacgtcat aactggcctt gt322 217 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 217acgcgggggg aagtgagcga cacactctgc gtcctcgcct caccagagtc ttgctgtgtg 60gcccaggctg gagtgcccgg ctggtctcaa attcctgacc tcaagtgatc tccctcccaa 120agtgttgcga ttgcaggtgt gagccactgc acctggctgc tgagaaatct ttgcctacag 180tgagggaaac tactaaagtt cctggggaag caaagtaaga atttcataag aacaaaatgg 240atggagagga gaaaacctat ggtggctgtg aaggacctga tgccatgtat gtcaaattga 300tatcatctga tggccatgaa tttattgtaa aaagagaaca tgcattaaca tcaggcacga 360taaaagccat gttgagtggc ccaagtcaat ttgctganaa cgaaaccaat gaggncaatt 420ttagagagat ccttcacatg tgctatcgaa agtattcatg nattttacgt accttgggcc 480gcgaccacct taaggccaat tncacacact ggcnggccgt actantggat ccnactngga 540ccaacttggc gtaatcatgg catactggtt cctggggaaa atgtatccgt tacaattcnc 600acacan 606 218 618 DNA Homo sapiens misc_feature (1)...(618) n = A,T,Cor G 218 ggtacttttt tttttttttt ttttttttga gacggagttt ggcccttgttgcccaggctg 60 aagtgcaata gtgcgatctc ggctcactgc aacctccacc ttccgtgttcaaccgattct 120 cctgcctcag cctcctgagt agctgggatt acagatgaaa aaacatttaaagcccttaag 180 gaagaaggaa atcaatgtgt aaatgacaaa aactataaag acgccctcagtaaatacagc 240 gaatgcttaa agattaacaa taaggaatgt gccatatata caaacagagctctctgttac 300 ttgaagctgt gccagtttga agaagcaaag caggactgtg atcaggcacttcagctagct 360 gatgggaacg tgaaagcctt ctatagacga actctggctc ataaaggactcaagaattat 420 cagaaaagct taattgatct caataaagtt atcctactag atccaagtattattgaggca 480 aagatggaac tggaagangt aactagactc ctaatcttaa ggataagacagcaccattca 540 acaaagaaaa ggagagaagg aaaatgagaa tcaagaggng aatgaaggcangaggancct 600 ggaaaacctg aggggagg 618 219 613 DNA Homo sapiensmisc_feature (1)...(613) n = A,T,C or G 219 ggtacaaagc ggatctgagcccggaaaatg ctaagctcct cagcacattc ctaaatcaga 60 ctggcctaga cgccttcctgctagagctgc acgaaatgat aatcttgaaa ctaaagaacc 120 cccaaaccca aaccgaggagcgcttccgcc ctcagtggag cctgagagac actctcgtaa 180 gttacatgca aactaaagaaagtgaaattc ttcctgaaat ggtatctcag ttcccagaag 240 agatactgct cgccagctgtgtctcagtgt ggaaaacagc tgctgtgctg aaatggaatc 300 gagaaatgag atagaattatttcctcagct atctttggat gactttggag agaagactcc 360 tctctcctcg tctgcggcgtggacttgatc atggactggt gcctttgcat tcagaaggag 420 agctgtcagc gtagcaccgaattcaagacc aaggcgtgct acctgagctg acagcttttt 480 gaaagccgag ctggttctgaaccatgtcct gcccnggcng gcgctcgaaa gggcgaattc 540 agccactggc ggccgtactantggatccga actcggacca aacttggcgt aatatgggca 600 tactggttcc tgg 613 220616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 220ggtacgcggg ggcagccgcg gtgttgtgct gtggggaagg gagaaggatt tgtaaacccc 60ggagcgaggt tctgcttacc cgaggccgct gctgtgcgga gacccccggg tgaagccacc 120gtcatcatgt ctgaccagga ggcaaaacct tcaactgagg acttggggga taagaaggaa 180ggtgaatata ttaaactcaa agtcattgga caggatagca gtgagattca cttcaaagtg 240aaaatgacaa cacatctcaa gaaactcaaa gaatcatact gtcaaagaca gggtgttcca 300atgaattcac tcaggtttct ctttgagggt cagagaattg ctgataatca tactccaaaa 360gaactgggaa tggaggaaga agatgtgatt gaaagtttat cangaacaaa ccgggggtca 420ttcaacagtt tanatattct ttttaatnnt ttcttttncc tcaatccttt tttattttta 480aaaatagttc ttttgtaatg tggtgtcaaa acggaattga aaactggcac cccatctttt 540gaaacatctg gtaatttgaa tctaatgctc attatcatta tggttggttt cattggcnga 600attttgggga tcaanc 616 221 615 DNA Homo sapiens misc_feature (1)...(615)n = A,T,C or G 221 ggtacagtga tagctccccc tgggcaatac aatacaagaacagtgggttt tgtcaaattg 60 gaacaaggaa acagaaccac agaaataaat acattggttaacatcagatt agttcaggtt 120 acttttttgt aaaagttaaa gtagagggga cttctgtattatgctaactc aagtagactg 180 gaatctcctg tgttcttttt tttttaaatt ggttttaattttttttaatt ggatctatct 240 tcttccttaa catttcagtt ggagtatgta gcatttagcaccactggctc aatgcgctca 300 cctaggtgag agtgtgacca aatcttaaag cattagtgctattatcagtt accaccattt 360 ggggctttta tccttcatgg gttatgatgc tctcctgatgacacatttct ctgagttttg 420 taattccagc caaagagaga ccattcacta tttgatggctggctgcatgc agacatttaa 480 agcttttaga gaatacacta caccagggag tatgactactantatgacta ttagganggt 540 aatacccaga attggactcg caccttaggc aagatccaaccactaaattg aataagaatg 600 agtngatgag gtncc 615 222 617 DNA Homo sapiensmisc_feature (1)...(617) n = A,T,C or G 222 ggtacttttt tttttttttttttttttttt ttttaattta tgattttatt gnctttcctt 60 tgtccggcct ttaacatgtttctgtaattt aaataaaaat ctatttactt tctccatttt 120 agcaaatggt ttctttacccaaataggttg cactatagtc cccatatggt tttctactgn 180 tccacaacca ctatttcacaaagattgaca aaactttaat aaaagttaaa tttacagaca 240 tcttaagata acttgggaaatatgtagtaa aaaagaatcg agtccacaaa ttaagaatat 300 tttgctaata tgcccaacaccaatttcagc aaatccaatc tacttaactc atatatttaa 360 tgnggtaatt tttctaacaaaatttaatgg gggtatgaat gatatattta tgcccttgac 420 aaagatgaca tgtgtgattttggtgngact aanaaaggag aagtatgatt tctggngggt 480 atganatcac tctggctcatcgaagctcca gaatatgtaa gggtctgnca cgtccaaaaa 540 tgttaggcna atgtataaaaggccacccgg ctnacacacg ttttatatac aaactttngn 600 agtcctttta tntcata 617223 470 DNA Homo sapiens misc_feature (1)...(470) n = A,T,C or G 223ggtaccacaa ctgtgccctt gataattagt aatcactcct aaaaatcttc atttggcacc 60agatggtgtg tttaaaacac cctaggatgt tttgaatcag gcttgatttt gttagttgag 120ttacaggaga attttaaggg tgagggtatg ggggtcaggg aagaaaagga aatgggaaat 180ggaccagaaa aaatcttgag tcatcatcta aatcaacaaa gcactgatag ctccaaatat 240taggtcagac actaaaacga ctgatatagg ctcaagtggt ttataaaacc tataaaaaga 300ctacaccagc aaagtccctg tcaatctgtc agagttcaga aactaaaaca gggagtaaca 360ttttagctta aaaccttatc tcaagagaat catatacact tcacatgaat aaaaatacct 420gaaaccaaac atttttaaaa gctccagtcc tgcccnggcc ggccgctcga 470 224 622 DNAHomo sapiens misc_feature (1)...(622) n = A,T,C or G 224 gcgtggncgcggccgacgtn ctcttttttt tttttttttt ttttttgcnn actaaaaatn 60 ngattgctctttaaagcctt aggccgnatg acaaaatgan nagactgaaa tgacancggg 120 gaggaagaaacagannaaag ataagaatga ggtggtcagg ttgggggaat taagcgaata 180 ttcncttccnnggtgagtcc tnacactggt ctcatgccca tgatgagttg cacaccaaac 240 acnggctgntgacttncctc ctgcnctant cagtgaactt gcngacatng ggnancctca 300 cattacagntataanntttc cacctaaaaa atgctgcgct tttcgacngg ctcnnncagn 360 ggccggggcttgacatggng gaanggattt ctctcccatg ccaaggaatt catcacatca 420 ctgntactccactgncaacc ttntccattg ggctcngtgc cctgtgtngg gtcatggacc 480 cantccanaantatgaatac tgtaccatgc tcttaaccag gaggacctaa ggatccttag 540 ncccntgagnnanacaccag gnttcaaagg ccgttttggn aagccaaatt tgnttnggnc 600 cgaattngggccaaacangg tt 622 225 619 DNA Homo sapiens misc_feature (1)...(619) n =A,T,C or G 225 acgcggggag ttccgccatg gcctccttgg aagtcagtcg tagtcctcgcaggtctcggc 60 gggagctgga agtgcgcagt ccacgacaga acaaatattc ggtgcttttacctacctaca 120 acgagcgcga gaacctgccg ctcatcgtgt ggctgctggt gaaaagcttctccgagagtg 180 gaatcaacta tgaaattata atcatagatg atggaagccc agatggaacaagggatgttg 240 ctgaacagtt ggagaagatc tatgggtcag acagaattct tctaagaccacgagagaaaa 300 agttgggact aggaactgca tatattcatg gaatgaaaca tgccacaggaaactacatca 360 ttattatgga tgctgatctc tcacaccatc caaaatttat tcctgaatttattagcccgt 420 ggggccaatt ttttaactca natcttgctg agaccaggag catctgatttaacaggaagt 480 ttcagattat acccgaaaaa gaagttctag agaaattaat agaaaaatgtggttctaaag 540 gctacgtctt ncaaatggag atgattggtc nggcaagaca gttgaatattctattggcga 600 ggttccatat canttgngg 619 226 277 DNA Homo sapiens 226acgcggggcc cctcatttac ataaatatta tactagcatt taccatctca cttctaggaa 60tactagtata tcgctcacac ctcatatcct ccctactatg cctagaagga ataatactat 120cgctgttcat tatagctact ctcataaccc tcaacaccca ctccctctta gccaatattg 180tgcctattgc catactagtc tttgccgcct gcgaagcagc ggggggccta gccctactag 240tctcaatctc caacacatat ggcctagact acgtacc 277 227 328 DNA Homo sapiens227 ggtacatatt tttgccaatg ctatacagca aaaatgaaaa acttacagaa aggtaaacaa 60aattgagtcc acttttttaa tttcacaagc tgctttaaac tatagaacca ccagatatct 120gtaaaataag caaaactggt aagtgtgttt ttttaattga gggaaggagg gccagaggag 180ttggtgcaga agcgcttcgg gtgaattcat accagagcca ccgggtgtga ctcggctacc 240tctcccaatt accacaggga ggtcttaaaa ttgaatttca gtttcagcag atactccaga 300tttacctgag caatatcata gacaatgt 328 228 609 DNA Homo sapiens misc_feature(1)...(609) n = A,T,C or G 228 acgcgggagt tcaagcagat gtatggctaaccggaaacag gtgggtcacc tcctgcaaga 60 agtggggcct cgagctgtca gtcatcatggtgctatcctc tgaacccctc agctgccact 120 gcaacagtgg gcttaagggt gtctgagcaggagaggaaag ataagctctt cgtggtgccc 180 acgatgctca ggtttggtaa cccgggagtgttcccaggtg gccttagaaa gcaaagcttg 240 taactggcaa gggatgatgt cagattcagcccaaggttcc tcctctccta ccaagcagga 300 ggccaggaac ttctttggac ttggaaggtgtgcggggact ggccgaggcc cctgcaccct 360 gcgcatcagg actgcttcat cgtcttggctgagaaaggga aaagacacac aagtcgcgtg 420 ggttggagaa gccagancca ttccacctcccttccccaac atctctcana gatgtgaaac 480 cagatctcat ggcaacnaag ccctntgcaagaagctcaag gaanctaagg aaaatggacg 540 ttttcagana atggttgtag ttcatgggttttncctactg ccgggtcctt tcttangacc 600 cgcanaant 609 229 610 DNA Homosapiens misc_feature (1)...(610) n = A,T,C or G 229 ggtacttttttttttttttt tttttttttt gcagactaaa aattttattg ctctttaaag 60 ccttaggccgtatgacaaaa tgaagagact gaaatgacag cggggaggaa gaaacagaag 120 aaagataagaatgaggtggt caggttgggg gaattaagcg aatattctct tccagggtga 180 gtcctcacactggtctcatg cccatgatga gttgcacacc aaacacaggc tgctgacttc 240 cctcctgcactagtcagtga acttgcagac atagggtaac ctcacattac agttataatc 300 tttccacctcagaaatgctg tgcttctcga caggctcgca cagtggccgg ggcttganat 360 ggtggagggatttctctccc atgcaaagta attcatcaca tcactgntac tccactccca 420 accttctccattgggctcgg tgccctgtgt ggggtcatgg acccaatcca acgtatgant 480 actggtaccaatgctnttac cagggaggac acnaaaggat cccttacccc ctgagcacag 540 acccnaggtttcaaanggcc gttttggcag gccaaactgn atntgnccag aatttggnga 600 caaaacaagg610 230 346 DNA Homo sapiens 230 ggtcggccga ggtaccatgc actgagtgactgtggggatc atgttgttat aatgaacaca 60 agacacattg cattttctgg aaacaaatgggaacaaaaag tatactcttc gcatactggc 120 tacccaggtg gatttagaca agtaacagctgctcagcttc acctgaggga tccagtggca 180 attgtaaaac tagctattta tggcatgctgccaaaaaacc ttcacagaag aacaatgatg 240 gaaaggttgc atctttttcc agatgagtatattccagaag atattcttaa gaatttagta 300 gaggagcttc ctcaaccacg aaaaatacctaaacgtctag atgagt 346 231 601 DNA Homo sapiens misc_feature (1)...(601)n = A,T,C or G 231 ggtacgcggg gagagcacat ccggtgttag aagcgctggtaggccttgga gaggcgggtt 60 aggaagagtg gagactgctg cacggactct ggaaccatgaacatatttga tcgaaagatc 120 aactttgatg cgcttttaaa attttctcat ataaccccgtcaacgcagca gcacctgaag 180 aaggtctatg caagttttgc cctttgtatg tttgtggcggctgcaggggc ctatgtccat 240 atggtcactc atttcattca ggctggcctg ctgtctgccttgggctccct gatattgatg 300 atttggctga tggcaacacc tcatagccat gaaactgaacagaaaagact gggacttctt 360 gctggatttg cattccttac aggagttggc ctgggccctgcctggagttt tgnattgctg 420 tcaaccccac atccttccac tgctttcatg ggcccgcaatgatctttacc tgcttaacct 480 taatgcactc tatccaagcg ccgtactcct tttctgggaggatcttgatg tcagcctgaa 540 cttgtgcttt gcttcctggg gaatgtttct ttggatccattggcttttca gcnaactttt 600 t 601 232 390 DNA Homo sapiens misc_feature(1)...(390) n = A,T,C or G 232 actttttttt tttttttttt ttttttttttttggttttaa tgtttatttc cccaagacag 60 cctagcctgc actctacttg gataaattttacaagctagt tttctgctgc ttctagtttt 120 aaactttaac catgtttctg atgacaaggaatgctgcaaa aatactctag ttcaacaaag 180 agttatgatc acaaaataat ttttatccattctacagtgt ttcanaatta ccagttgatt 240 tttaaacaca aagtagatat agatgctaatggtggctaat ctggtatgtt tcttatagca 300 aactgttgtt catgcaacac ttgtgctcaaaggggaaggc acaggatttc ctacaatgag 360 ccaccttata aagagttctt tttgnacctn390 233 603 DNA Homo sapiens misc_feature (1)...(603) n = A,T,C or G 233cgaggtacgc gggggaagag tgagggttcc aacttttctg cttatctggg aggtgttggg 60cgcggacaat cgagatgtca gagaaaaagc agccggtaga cttaggtctg ttagaggaag 120acgacgagtt tgaagagttc cctgccgaag actgggctgg cttagatgaa gatgaagatg 180cacatgtctg ggaggataat tgggatgatg acaatgtaga ggatgacttc tctaatcagt 240tacgagctga actagagaaa catggttata agatggagac ttcatagcat ccagaagaag 300tgttgaagta acctaaactt gacctgctta atacattcta gggcagagaa cccaggatgg 360gacactaaaa aaatgtgttt atttcattat ctgcttggat ttatttgtgt ttttgtaaca 420caaaaaataa atggtttgat ataagaaaaa annnnnnnna aaaaaaaagt nctggccngg 480cggccgttca aanggccaat tccacccact ggcggccgta ctaanggacc aacttggncc 540aacttgggga atcanggcaa actggttcct ggngaaatgg nttcccttcc aattccccaa 600atn 603 234 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G234 cgaggtacct tcattgcgat caaaccagat ggggtccagc ggggtcttgt gggagagatt 60atcaagcgtt ttgagcagaa aggattccgc cttgttggtc tgaaattcat gcaagcttcc 120gaagatcttc tcaaggaaca ctacgttgac ctgaaggacc gtccattctt tgccggcctg 180gtgaaataca tgcactcagg gccggtagtt gccatggtct gggaggggct gaatgtggtg 240aagacgggcc gagtcatgct cggggagacc aaccctgcag actccaagcc tgggaccatc 300cgtggagact tctgcataca agttggcagg aacattatac atggcagtga ttctgtggag 360agtgcagaga aggagatcgg cttgtggttt caccctgagg aactggtaga ttacacgaac 420tgtgctcana actggatcta tgaatgacag gaaggcagac ccattgnttt tcacatncat 480ttcccttcnt tccattgggc aaaggaccag ctttnggaaa tctantnttt accnggacct 540tattcttaat ttgganggaa actnttggac tttgangtnt tcctntacct ngcccgggng 600gccgtttaaa agggna 616 235 607 DNA Homo sapiens misc_feature (1)...(607)n = A,T,C or G 235 acgcggggag tgcgttactt acctcgactc ttagcttgtcggggacggta accgggaccc 60 ggtgtctgct cctgtcgcct tcgcctccta atccctagccactatgcgtg agtgcatctc 120 catccacgtt ggccaggctg gtgtccagat tggcaatgcctgctgggagc tctactgcct 180 ggaacacggc atccagcccg atggccagat gccaagtgacaagaccattg ggggaggaga 240 tgactccttc aacaccttct tcagtgagac gggcgctggcaagcacgtgc cccgggctgt 300 gtttgtagac ttggaaccca cagtcattga tgaagttcgcactggcacct accgccagct 360 cttcaccctg agcagctcat cacaggcaag gaagatgctgccaataacta tgcccgangg 420 cactacacca ttggcaagga gatcattgac cttgngttggacccaattcc aaacctggct 480 gaccatgcac cgggctttan ggnttnttgg gttttcccaaantttggggg ggaactgggt 540 ttgggttaac ttcctgntna tggnacgntt ttaaatgaatntgggaaaaa tccaactggn 600 gntttcc 607 236 608 DNA Homo sapiensmisc_feature (1)...(608) n = A,T,C or G 236 acgcgggcat gcaacaccacacccagcctg aaacccagat ttttaatatg aaatcaaagt 60 cttcagacct tgtaggtgtcataaaaagca cgctgaggac cactagtttg caactgccaa 120 tctaaaatat catagacattatatcacttc aaccacgaaa aaaaagtatg tgaggcagaa 180 aatggaagca accatgcctaatttattgtt gaatactttt tccgtatacc aagagcttcc 240 tttgcactag catctgaaactatatccaga atgacactgg ttttcataaa agtgttgatc 300 ctcacacctc tttatagtcttgcacctagc acagtggagt gaaacacttt aaatagcact 360 tgntccttga gtatatatggaaaaaagtga agtattgata aagtgctcaa ctaatatgag 420 cagcatctca ggagtctccaattcttgaat taccagggag tatttttacc attttcccca 480 ntgnaaggcc ttttttgaganacttaccct caaatngaan gnnttaagca tgntcctttt 540 tttttccttt tttttttganaaaagggctt gctntgtggc caggttggan tgcctacntg 600 aaaattcn 608 237 609 DNAHomo sapiens misc_feature (1)...(609) n = A,T,C or G 237 actatttcatatattgtgtg agccccacaa atgtctattt taaaaagagt atagtccctg 60 gccaggcgcggtggctcacg cctgtaatcc cagcagtttg ggaggccgag gtgggcggat 120 cacctgaggtctggagttcg agaccagcct gaccaatatg gtgaaacccc gtttctacta 180 aaaatacaaaattagctggg catggtggag catgcctgta atcccagcta ctcgggaggc 240 tgaggcaggagaatcacttg aacccgggag gcgaaggctg cagtgagcca agatcacgcc 300 attgcactccagcctgagca acaagaggga cactccgtcc ccaaaaaaaa aataataaaa 360 aaaataaaaaataaaaataa aaagagtata gttcccaatg ggttctacaa acattcctga 420 tttatactgggggaagtgat gcctaantgg gaacattaat cattatggtt tcgaaaatta 480 aatatttctgcaaacaattc ctttgcaaat gctaacttgc catgagctta ccccatttga 540 aattgngnctttacaaagac cttggccgga ccccttangg ngaattcagn cactggnggg 600 cgttctttg 609238 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 238acgaggcggt gcgggaagtc ctgcacggga accagcgcaa gcgccgcaag ttcctggaga 60cggtggagtt gcagatcagc ttgaagaact atgatcccca gaaggacaag cgcttctcgg 120gcaccgtcag gcttaagtcc actccccgcc ctaagttctc tgtgtgtgtc ctgggggacc 180agcagcactg tgacgaggct aaggccgtgg atatccccca catggacatc gaggcgctga 240aaaaactcaa caagaataaa aaactggtca agaagctggc caagaagtat gatgcgtttt 300tggcctcaga gtctctgatc aagcagattc cacgaatcct cggcccaggt ttaaataagg 360caggaaaagt tcccttcctg ctcacacaca acgaaaacat ggtggccaaa agtggatgag 420gtgaagtcca caatcaagtt ccaatgaaga aggggtatgt ctggcttgta acttgttggt 480cacgtgaaga tgacngacga tgacttgngt ataacattna nctgggctgg caacttcttg 540gggcaatgnt caanaaaact ggcaaaatgt ccgggccttt tttttagagc cccttggnaa 600accccangcc ntttta 616 239 607 DNA Homo sapiens misc_feature (1)...(607)n = A,T,C or G 239 acagtctgtt cgagaacacc ttggtcatga aagtgacaacctgctgtttg ttcagatcac 60 aggcaaaaaa ccaaactttg aagtgggttc ttctaggcagcttaagcttt ccatcaccaa 120 gaagtcttct ccttcagtga aacctgctgt ggaccctgctgctgccaagc tgtggaccct 180 ctcagccaac gatatggagg acgacagcat ggatctcattgactcagatg agctgctgga 240 tccagaagat ttgaagaagc cagatccagc ttccctgcgggctgcttctt gtggggaaag 300 ggaaaaagag gaaggcctgt aagaactgca cctgtggccttgccgaagaa ctggaaaaag 360 agaagtcaag ggaacagatg aacttccaac ccaagtcaacttgtggaaac tgctcctggg 420 cgatgccttt cgttgtgcca ctggccctac cttgggatgccagcntnaaa ctggggaaaa 480 gngcttctaa tgatancatc tttattgaag cctaagaaggttctgaattg ggacccattt 540 gttcttcaac caattctggn cttaaatcca ccttgggggtcttccacctc cttggatttg 600 ncacctt 607 240 615 DNA Homo sapiensmisc_feature (1)...(615) n = A,T,C or G 240 ggtacgcggg gcttttcacaagatggcgcc gaaagcgaag aaggaagctc ctgcccctcc 60 taaagctgaa gccaaagcgaaggctttaaa ggccaagaag gcagtgttga aaggtgtcca 120 cagccacaaa aagaagaagatccgcacgtc acccaccttc cggcggccga agacactgcg 180 actccggaga cagcccaaatatcctcggaa gagcgctccc aggagaaaca agcttgacca 240 ctatgctatc atcaagtttccgctgaccac tgagtctgcc atgaagaaga tagaagacaa 300 caacacactt gtgttcattgtggatgttaa agccaacaag caccagatta aacaggctgt 360 gaagaactgt atgacattgatgtggccaag gtcaacaccc tgattcggcc tgatggagag 420 aagaaggcat atgttcgactggctcctgat tacnatgctt tggatgttgc caccaaaatt 480 gggatcattt aactgagtccacttgctaaa tctgaatata tatatatata tatatctttt 540 cnccccaaaa aaaaaaaaaaaaaaaagtnc tncccggcgg ccgtttaaag gggaattccc 600 cacttggggg cgttt 615 241365 DNA Homo sapiens misc_feature (1)...(365) n = A,T,C or G 241acgggggggt cgctttgctg ttcgtgatat gagacagaca gttgcggtgg gtgtcatcaa 60agcagtggac aagaaggctg ctggagctgg caaggtcacc aagtctgccc agaaagctca 120gaaggctaaa tgaatattat ccctaatacc tgccacccca ctcttaatca gtggtggaag 180aacggtctca gaactgtttg tttcaattgg ccatttaagt ttagtagtaa aagactggtt 240aatgataaca atgcatcgta aaaccttcag aaggaaagga gaatgttttg tggaccactt 300tggttttctt ttttgcgtgt ggcaagtttt aaagttatta agtttttaaa atcaagtacc 360tnggn 365 242 625 DNA Homo sapiens misc_feature (1)...(625) n = A,T,C orG 242 natngganng nttttccctt aacgtgggcc ncggccgagg nacttttttt tttttttttt60 tttttttttt gcaggcagct atttaattan gntcttaana catttanaac nccaatttgn 120gaanataaat tccattcgtc anaacaaacn cagatcgcan gtagccctgg anctgangaa 180taactttgat ttttggnaaa atttgngagt ccncagcttt ctgatcaatc ttgcgctgct 240cccnaatctc atatttctct ttttctgggg ccaaaatctt accttcctgg ngtctgggct 300ttcgcaactt cttcttcttg aaagaagcct cagtaaaaat ggtttgggaa ttttacatta 360ctgatatcca atttnggtga aatggcaatg accaatttct nggggggtct tcgtaaaaga 420actccantga nggnccaaag gtccagtccc aagtataggc nctnaccact gnttcaggaa 480accacctttt gncctggggg gtccatgagg atgaccaaat ggncccgggg naagctggct 540ccantttttt acggcctacc gaagggtttt tgccngggta aaagttttag ggccattttc 600ngggnaaatc taggcttttg gaaat 625 243 639 DNA Homo sapiens misc_feature(1)...(639) n = A,T,C or G 243 nncnaattcc nccntaaccn ggnccccgnccaagnacccc ggcncctttg gatgtatnga 60 aatnaacnta ttaatgggga cntattggagaaggaaatnc ctagacctac aactttnagc 120 naatagcngt gatgttttag gaactgaaatgtcacactta aagtcttnag cccagctact 180 tccctatttt tgtggggaga aaanggccngattagaactg ttctggttgt gtttggcggg 240 aggggaataa tttttgttca gtccttcttagtgaccaaac tttaattttt aagaataata 300 tattgactta ctgaactgaa gcattctgagttgaaaggag ctccncagga ntggagttct 360 gtgttgctca catgttnaaa ncttgctcaccttnatagcn caaggaatac ctatcttcca 420 natnccgcca ttttcatctc ttaaatgnagtccaaagtat gacttgagaa agttgctctn 480 ggattctggg gtcttaaaac tngggattctgggattntgg ggtccnaaag ttnaccttgn 540 aaagttgcct gggnttttan aaatncnctgnattctgggg ttttaaaaaa ttttgaaaaa 600 accccncccn ncttgaaagg gaccttaaaaattaacctn 639 244 614 DNA Homo sapiens misc_feature (1)...(614) n =A,T,C or G 244 tcgagccgnc ggcccgggcc aggtactttt tttttttttt ttttttttttgaaaatggag 60 tcttgctctg ntgccaaact ggantgcaat ggtgcganct gggctcactgnaatctccac 120 ctnccgggtt caagcgattc tcctgcctca cctccgagta actgggactacaggtgcgcg 180 ccaccaagcc cagctcattt ttgnattttt agtanaaatg gggtttcacgatgttggcta 240 ngatggnctc gatctctggt caaagtcttt tctgnaaata tccttggtaaaaaaacaatt 300 ttagactgta gctgttgcaa atgctttaag gaagaaacna aacaactgcagtcttcctga 360 aatgaaaaaa ctccccaggg ctgctattna aaacaacccc accagcacttcaatcatgat 420 gccnacagtg gcccactgaa aaancnggaa aagttcnaat cccaaactgggatgctcttg 480 actntggaat tntgngggcn ntncccnant ttnanacaaa acngnctnggnccctntttt 540 ttgggggaat ttgggaanaa aaaaacttgn gngttcttgn ggttccnttgttccccaaaa 600 nactgggggn nggg 614 245 620 DNA Homo sapiens misc_feature(1)...(620) n = A,T,C or G 245 gccgtggtcg cgggccgagg tccatttgcctcccggcctc aagccgattc tcctgcctca 60 gccctccaag tagctgggga ttacaggcacctgccaccat gcccggctaa tttttgnaat 120 tttagtagag acagggtttc accatgttgcccaggctggt ttcgaactcc tgacctcagg 180 tgatccaccc gcctcggcct ccaaagtgctgggattacag gcttgagccc ccgcgcccag 240 ccatcaaaat gctttttatt tctgcatatgttgaatactt tttacaattt aaaaaaatga 300 tctgntttga aggcaaaatt gcaaatcttgaaattaagaa ggcaaaaatg taaaggagtc 360 aaaactataa atcaagtatt tgggaaagtgaagactggaa gctaatttgc attaaattca 420 caaactttta tactctttct ggatatacattttttttctt taaaaaacaa ctttngatca 480 gaatagcccc atttagaacc ttttggtatcagncaatatt tttaaatagt tnaaccnggc 540 ctaagctnaa agnggcttga tntgagtaaacttttcaact ggcttgaacc ctnaaccttt 600 taaaatgacc ttccgagntt 620 246 595DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 246 acttattcttcaggggttac tgagtcggca cctatgacag ctaagagagc tttcttaaag 60 actgcctcagtgtcttcttg gcttttggca ccttcactcc actctgccca ggaaatccac 120 aatggcagacaaacctgggg tttcaggtgc acaaagactt cttcaaaaag catggctatg 180 tcagggctctttgactcgat cagcacctgc agcttcagct gccacattgt cccagagtct 240 ctaaacaattcaagttccag ctactgncac ttccagagct tcctcaggaa gttataacac 300 agcaacgaaacactcaactg cttgtattgg cattctgaca gaagcttcaa gttcatgtgc 360 cttcctgaatacagtcatgg tctttncaac ctcttcctct aaggacccac tatttgactt 420 cttaataaatctttccagcc aaaggngatg aacactttca catgggcctt gtggcaaaag 480 cttnatggctttttatcncg gacagacctt tctcttcggg cgacctcaat ggtttggctt 540 ggtcgtggagctggtntttg gctnggactc aacttnaatn ttgcttgccc naaac 595 247 364 DNA Homosapiens 247 gggtacacta gaaagtcttt tacaaaataa tcatcttaga tcaacagaagaccaatcttc 60 aatgtcgtcc tgcaagatgg gttactttaa catctcctcc tgttttctccaatgttctcc 120 tttagtatgg ctggtaattg ttttggtgat tgccaccccc tcgagatgccttgccataag 180 tgctctgttg gccactgtag tctgcatatc cctgtccata tccatagttcccatagttat 240 acccagtata atcatatccg ccatagccac tatagttttg atcaccaccataggcactat 300 tgtaatttcc atatccttga tcataatagt tattaaatcc ttggttccagttttggccct 360 gacc 364 248 591 DNA Homo sapiens misc_feature(1)...(591) n = A,T,C or G 248 ggtncagata tcttcaaagg aggaagaagaaagggaaacc agatggtgga cctgaatatg 60 ncccttancc aganctaatc aacccactcagccagaatag aagaagctgg aatagattcc 120 ccaacctggt ttgccagttc atcttttgactctattaaaa tcttcaatag ttggtattct 180 gnaatttcac tctcatgant gcnactgnggcttaactaat attgcaatgn ggcttgaatg 240 taagtagcat cctttgatgc ttctttgaaacttgnatgaa tttgggtatg aacagattgc 300 ctgctttccc ttaaataaca cttaaaattatttggaccag tcagcacaac atgcctnggt 360 tgnattaaag cnnggatatg ctggattttataaaattggc caaattagag aaatntagtc 420 ccatggaaat atatttcttg taaaaaagtgcttgaatctt tttggtcaag ataatgccac 480 tcttaagaat atcttcncac tnttganggattaaatatcg gcantggaaa agccttaaaa 540 atggggtcna cttgccttgn gcctaaaccgaccctgaaat gggatttccc n 591 249 332 DNA Homo sapiens misc_feature(1)...(332) n = A,T,C or G 249 actctccgag agggtcgttt tcccgtccccgagagcaagt ttatttacca aatgttggag 60 taataaagaa aggcagaaca aaatgagctgggctttggaa gaatggaaag aaagggctgc 120 ctcaagagct cttcagaaaa ttcaagaacttgaaaggaca gcttgacaaa ctgaagaagg 180 aaaagcagca aaggcagttt cagctttgacagtctcgagg cttgcgcttg cagaaacnaa 240 aacagaaagg ttgaaaatga aaaaacccagggtaccttgg nccgggacca cgcttaaggc 300 gaaattccaa cacacttggc cggccggtac ta332 250 626 DNA Homo sapiens misc_feature (1)...(626) n = A,T,C or G 250ggtactatta gccatggtca accccaccgt gttcttcgac attgccgtcg accggcgaag 60ccctttggcc cgcgtcttcc tttgaactgg ttgcagacaa gggtcccaaa ganagcagaa 120aattttcgtg ctctgagcac tggagaaaaa ggatttggtt ataagggttc ctgctttcac 180agaattattc cagggtttat gtgtcaaggt ggtgacttca cacgccataa tggcactggt 240ggcaaagtcc atctatgggg aagaaatttg aagatgaaga acttcatcct aaagcatacg 300ggtcctggca tcttgtccat ggcaaatgct ggacccaaca caaatgggtc ccaatttttc 360atctgcactg gccaagactg antggttgga tggcaaanca tgtngtgntt ggccaaagtg 420aaagaaggca tgaatattgt ggaaggccat ggaacgcttt tgggtncnag gaatggcaag 480aaccnccagg aagaatcacc cnttnttgac tggggacaac tcnaataagt tgacttgggg 540nttaatntaa ccccccanca attccttttg gaactcagga aacacccttc ancccanttn 600tttcaanttc caaaannttg ggcctn 626 251 603 DNA Homo sapiens misc_feature(1)...(603) n = A,T,C or G 251 actttttttt tttttttttt tttttttttcaacagaagaa cttttngttt ctttattttc 60 aatattngtc ttattaatat ttttcttattttataatgca attacaacaa tttaggagac 120 aaaacantat aaacaaaaga atgttaaatagtttttttta aaaaatagct tgttgcttgc 180 aagaaagtcc atataatctt attcccccccaaatataatt ttatactttg cactaaacca 240 aaatagctta tggaaaatta ggtattaaatagctaaacac agaaaaccta cagctataaa 300 taacataaaa tacagtttaa ctttaatgngatgcttaaac aaagcaaact atgatgcant 360 atgaatcaac ttcattaatt ggacaagtccagtgaggcnc aaattagata agcnctaaac 420 cctcatgatg ggcaagtgaa accttcaccccagcaagggt ctttcnggtc ttggctatgc 480 caattccttc canaaaagnc ccagttttacangtctggct ttttccgggg gaacccccca 540 tttntttnnc ccaagttggt tnggatttggcccccannaa attttttttg gngnaaaaan 600 aan 603 252 500 DNA Homo sapiensmisc_feature (1)...(500) n = A,T,C or G 252 actttatttg ttttttttgttttgttttgg tttttttttt ggcttgactc aggatttaaa 60 aactggaacg gtgaaggtgacagcagtcgg ttggagcgag catcccccaa agttcacaat 120 gtggccgagg actttgattgcacattgttg tttttttaat agtcattcca aatatgagat 180 gcattgttac aggaagtcccttgccatcct aaaagccacc ccacttctct ctaaggagaa 240 tggcccagtc ctctcccaagtccacacagg ggaggtgata gcattgcttt cgtgtaaatt 300 atgtaatgca aaatttttttaatcttcgcc ttaatacttt tttattttgt tttattttga 360 atgatgagcc ttcgtgcccccccttccccc ttttttgtcc cccaacttga gatgtatgaa 420 ngcttttggt ctccctgggaagtgggtgga ngcagccagg gcttacctgt accttggccg 480 cgaacaccta aggccaantt500 253 634 DNA Homo sapiens misc_feature (1)...(634) n = A,T,C or G 253tcgagcggcc ngcccgggca ggtactatta gccatggtca aaccccaccc gtgttcttcg 60acattgcccg tcgacggcga acccttgggc ccgcgtctcc tttgagctgt ttgcagacaa 120ggtcccaaag acagcagaaa attttcgtgc tctgagcact ggagagaaag gatttggtta 180taagggttcc tgctttcaca gaattattcc agggtttatg tgtcaggggt ggtgacttca 240cacgccataa tggcactggt ggcaagtcca tctatgggga gaaatttgaa gatgagaact 300tcatcctaaa gcatacgggt cctggcatct tgtccatggc aaatgctgga cccaacacaa 360atggttccca gtttttcatc tgcactgcca agactgantg gttggatggc aaacatgtgg 420tgtttggcaa antgaaagaa ngcatgaata ttgtggaagc catgganccc tttnggtcca 480ggaatggcag aacnnccagg aanacaccct tgntgactgt ggcaactcga ataaattgac 540ttggggttat cttaaccncc caacattcct ttggacttag gaancanccc ttcancccnt 600tggttcaant tcccaaaaat ttgggctncc tnng 634 254 602 DNA Homo sapiensmisc_feature (1)...(602) n = A,T,C or G 254 nctttttttt ttttttttttttttttaaat taattaatta aaaaataggt ggnctactgg 60 tggtccttaa gctggaantgcagtgggcac aatcatggnt cactgnagtc tnaacctncc 120 aggttcaagt gatcctcctacctcacctcc antagctggg attacaggca tatgcgacca 180 tgcccagcta attttttattttttgtaaaa acggggtctc actatgtcgc ccangctggn 240 cttgaactcc tgaactcaagtgacccttcc gnctnacctn caaagtgcta ggcttacagg 300 tgtgaaccac catgcctggcctaaaaaatt tattttaaaa aagtaattta tctcttacag 360 ttgtggaggc tgagaaatccaangncaant ggcncatttg gtgaaaacct tnttgctggt 420 ggggactctg tgaaatncccaantggcnca tgcatnacac antgangggg cttacattcc 480 aacatgctat ctcttttaagttttaaagta cnggccnaaa tntgaacntg aatgacttna 540 aatccacnca ttccncttttggacnaaaaa ccntgggcaa ttgggatctt ggcnttttna 600 aa 602 255 614 DNA Homosapiens misc_feature (1)...(614) n = A,T,C or G 255 cgaggtacaggtaagccctg gctgcctcca cccactccca gggagaccaa aagccttcat 60 acatctcaagttgggggaca aaaaaggggg aagggggggc acgaaggctc atcattcaaa 120 ataaaacaaaataaaaaagt attaaggcga agattaaaaa aattttgcat tacataattt 180 acacgaaagcaatgctatca cctcccctgt gtggacttgg gagaggactg gaccattctc 240 cttagagagaagtggggtgg cttttaggat ggcaagggac ttcctgtaac aatgcatctc 300 atatttggaatgactattaa aaaaacaaca atgtgcaatc aaagtcctcg gccacattgt 360 gaactttgggggatgctcgc tccaacccga ctgctgtcac cttcaccggt ccagttttta 420 aatcctgagtcaagccaaaa aaaaaaaacc anaccaaacn nanaaaccaa ttaagccatg 480 ccaatctcatctggtttctg cncaagtang gttgncaaaa aagggttacc ncactaantc 540 ntagcccctaaaccnttgcg ggggncantg angggccgan tttganactc cggntggtga 600 nccanttggnggag 614 256 308 DNA Homo sapiens misc_feature (1)...(308) n = A,T,C orG 256 ncntccagca gtgggtcatt cgncaacgaa agtcntaccg tagaaaagat ggcgtgtttc60 tttattttga agataatgca ggagtcatag tgaacaataa aggcgagatg aaagggtctg 120ccattacagg accagtagca agggaatgtg cagacttgtg gccccggatt gcatccaatg 180ctggcagcat tgcatgattc tccagtatat ttgtaaaaaa taaaaaaaaa ctaaacccaa 240aaaaaaaaat nnnannnaac annnnanaaa aannnnaaaa aaaaaaagta cctnggccgn 300gaccacgc 308 257 602 DNA Homo sapiens misc_feature (1)...(602) n = A,T,Cor G 257 gcgtggtcgc nggccgaggt acgcggggga gacaaaccat accatatcccaccagagagt 60 cgcagacact atgctgcctc catggccctg cccagtgtat cttggatgctgctttcctgc 120 ctcatgctgc tgtctcaggt tcaaggtgaa gaaccccaga gggaactgccctctgcacgg 180 atccgctgtc ccaaaggctc caaggcctat ggctcccact gctatgccttgtttttgtca 240 ccaaaatcct ggacagatgc agatctggcc tgccagaagc ggccctctggaaacctggtg 300 tctgtgctca ntggggctga gggatccttc gtgtcctccc tggtgaagagcattggtaac 360 agctactcat acgtctggat tgggctccat gaccccacac agggcaccgaacccaatgga 420 aaangntggg antggaataa cantgatgtg atgaattact ttgcatgggagagaaatcct 480 tcancatttt naaccccggc cctgtccaac ctntcaaaaa cncacatttttaaggggaaa 540 attttactgg atggganggt acccttttnt ggaagtactg cttttcngganggaagtacc 600 cc 602 258 600 DNA Homo sapiens misc_feature (1)...(600)n = A,T,C or G 258 ggtgtntgng ncttatntgt agcggcgcgg ntggttctgaaatcgccttc agcggcgccg 60 cagtcntatt atgtgnatgt ccctaccacn aaaatncagattaattggna tgctcattac 120 ccacgtgaac gccaaagccc ttcgaagtag tgctgccctgcactnaatca agaagttgca 180 ttaaaattag aaccaaatcc agagtcactg gaactttcttttaccatgcc ccanattcag 240 gatcagacac ctagtccttc cgatggaaag cactagacaaagttcacctg agcctaatag 300 tcccagtgaa tattggtttt atggggatag gtgatatggncaatgaattc aagttggaat 360 tggnagaaaa actttttgct naagacncng aagcnaagaacccattttct actnaaggca 420 cagatttaga cttggagatg gtagcttcct atatccaatggatgatgctt tcagtccgtn 480 cnttgatcag tgncacnttn gaaagcagtt cccaagncctgnaacccagt cctaagccaa 540 gtccggttcn gcgattaatc cgactatgta tgcccttcatngcccctgtn ataaacnggn 600 259 600 DNA Homo sapiens misc_feature(1)...(600) n = A,T,C or G 259 gccgaggtac atgggaaagg gagtatggngagctatttcc tttttaaagg atgaagacct 60 tcataaattg gcccctcgga ttctggtgattcccgcccgc aagcgcaaat gctccagtgn 120 gttatgaaaa tgnttgntaa tctgctctggttcttcactg gattcaagan tcgggaggnc 180 ttctcgaatc ttttggataa nctggtttaaaacctgaatt gntacccgca tcattttcct 240 tttcataaaa atagatatat ctgntcagaatttctatnaa aagctgcact tgtaganang 300 ggtccatgca ctgatttgct atttttaaagctttttttan gcactccatt accctnttgc 360 cttcgtgaaa cttcttccca tttttgnccnggttctggcn gaccngaaga aatgtgccca 420 agtgcttaca agttnggcct gacaaggttcnttaaaantt tggatgtacc aagggccccc 480 tgggtcctca aaggtcatga atctttttactggaaccctt atcctttnaa aaggccatgg 540 tcaagggaat gnncttcttg gctttgaaacccggattaan tttttncaaa aaaagccngn 600 260 593 DNA Homo sapiensmisc_feature (1)...(593) n = A,T,C or G 260 acgcgggaac tccatcctcaccacccacac caccctggag cactctgatt gtgccttcat 60 ggtagacaat gaggccatctatgacatctg tcgtagaaac ctcgatatcg agcgcccaac 120 ctacactaac cttaaccgccttattagcca gattgtgtcc tccatcactg cttccctgag 180 atttgatgga gccctgaatgttgacctgac agaattccag accaacctgg tgccctaccc 240 ccgcatccac ttcctctggccacatatgcc cctgtcatct ctgctgagaa agcctaccat 300 gaacagctta ctgtagcagagatcaccaat gcttgctttg agccagccaa ccagatggtg 360 aaatgtgacc ctcgccatggtaaatacatg gcttgctgcc tggtataccg tggtgacntg 420 ggtnccaaag atgtcaatgctgccttggca ccattcaaac caagcgcaga ttcaatttgg 480 ggatggtgcc cactggctttaaggtngnat naactaccag cttccactgn ggnnctggtg 540 gaaactngcc aaggnnccttggccggaaca ccctangggg aattcanncc act 593 261 343 DNA Homo sapiensmisc_feature (1)...(343) n = A,T,C or G 261 cctacctctc ttnccactgcaaatttctgg gatagaccaa aagtgaattt gattatgtgt 60 tggctgaagt tcttcattctgactgttgan gggaggtttt cctttgaaga gttttcatcc 120 cagactcagc tgtcttttcacatggatgaa ataattcctg ctaccaacaa cagagcttca 180 ccaggaagtt gagttttcaagatgccttgt tgctttgaag aagggagtga tgtcaattct 240 cttgntacat tctccctttagcaacctgag taagagactc tctgccactg ggctgcaaaa 300 aaataaatta cttgaatctccccttggccc angctgaggt acc 343 262 593 DNA Homo sapiens misc_feature(1)...(593) n = A,T,C or G 262 actttttttt tttttttttt tttttttgtttttttttttt tttttttttt tttttttttt 60 tttttttttt ttacagngtn ttttcatttttattactcaa aaaagtttca tttttttnat 120 ttanctttnt gactntgggc ttgggccttnaacantttca naacgatttt ntgctcctcg 180 anaaggaaag cncccttgat cctgncacnaacncntttag cncacatgga accnccatag 240 gccctgntga catgtttctt tgtttnggacaatntcataa aaactttagg nnttacagca 300 cnaacccctn naagtntgcc tgggcncacaccanatgcaa attttggggc tttcccaacc 360 ttnttggnat aaaggtaaac aattttattaccagggggtt cgggacaacc tanttttgtt 420 aaaggctgta ttgtaggaaa acctacctcgggatgtcaaa cccttnacca ttttgagggn 480 ctggaaanaa ngttcccgga aanccccgggtancttnggc cggaaccccc taangggnga 540 attccnaccn cttgggggcn gtantaagggganccaantt gggccaaant tgg 593 263 591 DNA Homo sapiens misc_feature(1)...(591) n = A,T,C or G 263 accaagagtt tgctcctggc tgctttgatgtcagtgctgc tactccacct ctgcggcgaa 60 tcagaagtaa gcaactttga ctgccgtcttggatacacag accgtattct tcatcctaaa 120 tttattgtgg gcttcacacg gcagctggccaatgaaggct gtgacatcaa tgctatcatc 180 tttcacaaag aaaaagttgt ctgtgtgcgcaaatccaaaa cagacttggg tgaaatatat 240 tgtgcgtctc ctcagtaaaa aagtcaagaacatgtaaaaa ctgtggcttt tctggaatgg 300 aattggacat agcccaagaa cagaaagaaccttgctgggg ttggaggttt cacttgcaca 360 tcatggaggg tttaatgctt atctaatttgtgcctcactg gacttgncaa ttaatgaagt 420 gatcatattg catcataagt ttgctttggttaancttaca ttaaagttaa ctggatttta 480 agggaattat actgtaggtt ctggggtaactatttaatac taattttcat aacnattttg 540 gttaatncca agttnaaatt tatttggggggaanaaaatt tttggccttc t 591 264 595 DNA Homo sapiens misc_feature(1)...(595) n = A,T,C or G 264 accaagagtt tgctcctggc tgctttgatgtcagtgctgc tactccacct ctgcggcgaa 60 tcagaagtaa gcaactttga ctgccgtcttggatacacag accgtattct tcatcctaaa 120 tttattgtgg gcttcacacg gcagctggccaatgaaggct gtgacatcaa tgctatcatc 180 tttcacaaag aaaaagttgt ctgtgtgcgcaaatccaaaa cagacttggg tgaaatatat 240 tgtgcgtctc ctcagtaaaa aagtcaagaacatgtaaaaa ctgtggcttt tctggaatgg 300 aattggacat agcccaagaa cagaaagaaccttgctgggg ttggaggttt cacttgcaca 360 tcatggaggg gtttagtgct tatctaatttgtgcctcact ggacttgtcc aattaatgaa 420 gttgattcat attgcatcat agtttgctttggttaagcat cacattaaag ttaaactgga 480 ttttatggta tttatagctg nanggtttctggggttanct atttaatact aaatttccat 540 aagctttttg ggttaangcc aagnttaaaatttttttggg ggggaaaaaa atttt 595 265 592 DNA Homo sapiens misc_feature(1)...(592) n = A,T,C or G 265 ggtacttttt tttttttttt ttttttttttttgaaaatta tacttttatt tgagtcacca 60 ggagaaagat tcacttgtgg ttcaagtcaaatgttcanaa tcataacagg ccanaaaggt 120 ttgatcccga gcacaagccc acgagggaggggaccaaaac agaccaaaat gagacaacaa 180 ccccatataa aaagatgaac tggcggcttcacacactcac acacatacac atacacacgg 240 atgaaatgtt tggacagagg caaatttcacgtggtcattt ctgtttcttt ttaaatacag 300 gtttgtgggg tggtattttg ttttttccagctataaaaaa aggcccaaaa gtgcatgtgt 360 gaggggggaa aggcagaaat taagcaataaagtcattttc cctggaggga catganaggg 420 agaaaacagg aggcaattgc tggganaacgcactttctta acactgggct tttgggtatt 480 cttantattg gnccncaaaa agttattttcacattctaac tttgaagnct ntttccnggg 540 attnaatggn ccttaaaacc tttgggaactttaaaaaaac cngggcttac cc 592 266 594 DNA Homo sapiens misc_feature(1)...(594) n = A,T,C or G 266 acgcggggaa aaaaaaggca gtattccctttttaaatgag ctttcaggaa gttgctgaga 60 aatggggtgg aatagggaac tgtaatggccactgaagcac gtgagagacc ctcgcaaaat 120 gatgtgaaag gaccagtttc ttgaagtccagtgtttccac ggctggatac ctgtgtgtct 180 ccataaaagt cctgtcacca aggacgttaaaggcatttta ttccagcgtc ttctagagag 240 cttagtgtat acagatgagg gtgtcccgctgctgctttcc ttcggaatcc agtgcttcca 300 cagagattag cctgtagctt atatttgacattcttcactg tctgttgttt acctaccgta 360 gctttttacc gttcacttcc ccttccaactatgtcccaga tgtgcaggct cctcctctct 420 ggactttctn caaaggcact tgacccttcggnctctactt ggcccctnac ctcaccccct 480 tctggcaccg gncntgngac attcacttcngagaagaccn cccccaagga ggcnggcgnt 540 tggnccanga aaaaaccccg gggaagggtttntttttttn aaagggaaat ttcc 594 267 598 DNA Homo sapiens misc_feature(1)...(598) n = A,T,C or G 267 actggccctc ggtgctggca aaggtgtagttccactggcc gagggaatca agacatagtg 60 gtccttctgc taagccaagg gctgccacaatgacacagta gccagatcct gcaattccaa 120 tgagagcagc caatacagaa gaaagcatcgcacatcgttt gccacagttt tcatggccac 180 agcagccaca gcagtcatcc tgttccagcccaatgaagac aaatgctggc aggagcatca 240 gcagggccac ctcctacgat gccagaaaagaaccacacga aacggctgag gtggttttcg 300 gaggcatact ttgttcccat tgggaaagtaaagccaaata ttacccgcga tgcacaggaa 360 ggggcgagcc caaccagaaa atgtccgaatgcatcgtgca cacttcccat agcacatggt 420 ggtcttgcta ggtttttctc ccccttctctttggncttca acttcagtga taccccaaat 480 tagatgaaag tggtgccctt ttgggtggaaaaagcaaaca ccaaccccgg gtacctttgg 540 gccggaacac ncttaaggcc aattccanncaattggcggc ccgtacttan gggatccc 598 268 590 DNA Homo sapiens misc_feature(1)...(590) n = A,T,C or G 268 ggacatatta tcaataggct ataagatgtaacaacgaaat gatgacatct ggagaagaaa 60 catcttttcc ttataaaaat gtgttttcaagctgttgttt taagaagcaa aagatagttc 120 tgcaaattca aagatacagt atcccttcaaaacaaatagg agttcaggga agagaaacat 180 ccttcaaagg acagtgttgt tttgaccgggagatctagag agtgctcaga attagggcct 240 ggcatttgga atcacaggat ttatcatcacagaaacaact gttttaagat tagttccatc 300 actctcatcc tgtattttta taagaaacacaagagtgcat accagaattg aatataccat 360 atgggattgg agaaagacaa atgtggaagaaatcatagag ctggagacta cttttgtgct 420 ttacaaaact gtgaaggatt gtggtcacctggaacaggtc tncaatctat gtagcactat 480 gtggctcanc cttggtaccc cttggattatatatcaacct gnaacatgng nctgggactt 540 actttcnaaa cnaaatnttc cttntttgaagaaaatctgg gtttttgnaa 590 269 602 DNA Homo sapiens misc_feature(1)...(602) n = A,T,C or G 269 acttgaagga agtcgaatca gagatagactctgaagaaga acttataaat aaaaaaagaa 60 tcatagagaa agttattcat cgactcacacactatgatca tgttctaatt gagctcaccc 120 aggctggatt gaaaggctcc acagagggaagtgagagcta tgaagaagat ccctacttgg 180 tagttaaccc taactacttg ctcgaagattgagatagtaa aagtaactga ccagagctga 240 ggaactgtgg cacagcacct cgtggcctggagcctggctg gagctctgct agggacagaa 300 gtgtttctgg aagtgatgct tcaggatttgttttcagaaa caagaattga gttgatggtc 360 ctatgtgtca cattcatcac aggtttcataccaacacagg cttcagcact tncntttggt 420 ggtggttcct ggtcccntgg aagttggaaccaaattaatg gngtagtctc tatacccaat 480 acctttggtt ttcatgtgta anaaaaaggnccattacttt taanggattg tgctggnctt 540 attgngccan taactttttt ttaaatggcccagttacngg ttttaattct taaaannaaa 600 aa 602 270 595 DNA Homo sapiensmisc_feature (1)...(595) n = A,T,C or G 270 ggtacgcggg ggtaggagcctctctcccta ctgctgctac acaagaccct gagactgacc 60 tgcaggacga aaccatgaagagcctgatcc ttcttgccat cctggccgcc ttagcggtag 120 taactttgtg ttatgaatcacatgaaagca tggaatctta tgaacttaat cccttcatta 180 acaggagaaa tgcaaataccttcatatccc ctcagcagag atggagagct aaagtccaag 240 agaggatccg agaacgctctaagcctgtcc acgagctcaa tagggaagcc tgtgatgact 300 acagactttg cgaacgctacgccatggttt atggatacaa tgctgcctat aatcgctact 360 tcaggaagcg ccgagggaccaaatgagact gagggaagaa aaaaaatctc tttntttctg 420 gaggctggca cctgattttgtatccccctg tagcagcatt actgaaatac ataggcttat 480 atacaatgct tctttctggatattctcttg gcttgggtgg accccttttt ccggccccag 540 aattgttaan taatngaannnccntncann aagggnnnaa aggnaaatca ncttt 595 271 592 DNA Homo sapiensmisc_feature (1)...(592) n = A,T,C or G 271 ggtacattga gatcccgcctctacaaaatc aaaaaattag ccaggcaagg tggtgcgtgc 60 ctgtcgcccc agctacttggcaggctgagc tcaggaggtc aagcctgcct tgggccatga 120 tcatcccatg cactccagcctgacattcag agcaagacct tgtctcaaag aaagaaaaac 180 atttttatgg tgttttcttttttagtcttt tcaataatga aaattttcat tttacaggta 240 aaatgaaagg cctggcatttattcaagatc ctgatggcta ctggattgaa attttgaatc 300 ctaacaaaat ggcaaccttaatgtagtgct gtgagaattc tcctttgaga tttcagaaga 360 aaggaaacaa tgtgattcaagatatttaca taccagaagc atctaggact gatggatcac 420 tgtcccgatt caaattattcttcagtccat ttcccctttc tatttcagct ggtccttttc 480 acctaactgt cagtcattctggtttcaacn atgctttatc tcatgtcctt gaatatagtt 540 ggggnacttt aatttttanggaataatnna acagnttccn ttaaaggntn ng 592 272 607 DNA Homo sapiensmisc_feature (1)...(607) n = A,T,C or G 272 acattaaagt gtgatacttggttttgaaaa cattcaaaca gtctctgtgg aaatctgaga 60 gaaattggcg gagagctgccgtggtgcatt cctcctgtag tgcttcaagc taatgcttca 120 tcctctctaa taacttttgatagacagggg ctagtcgcac agacctctgg gaagccctgg 180 aaaacgctga tgcttgtttgaagatctcaa gcgcagagtc tgcaagttca tcccctcttt 240 cctgaggtct gttggctggaggctgcagaa cattggtgat gacatggacc acgccatttg 300 tggccatgat gtcaggctcggcaacaggct ccttggtgac actcaccaca ttgnttttca 360 agctgacttt cagcttgncaccttggagag actttaaccc ggaccaaggg cccgatgcct 420 tccgttaccc aggaatttcatcaccaatgg tggtanttca ggaatgttgg caagtttcct 480 tggcatnttc ccaaanagtttgttcccgtt cttnttgggn ggcangggct tcggaaaggg 540 ttnattttgt ngggaaccnaaaaactgggg tnaaactcct tnccggttna ngggtttccg 600 nnanccn 607 273 398 DNAHomo sapiens misc_feature (1)...(398) n = A,T,C or G 273 ggtaccgccattattctttt gggcaccttt ggttgttttg ctacctgccg agcttctgca 60 tggatgctaaaactgtatgc aatgtttctg actctcgttt ttttggtcga actggtcgct 120 gccatcgtaggatttgtttt cagacatgag attaagaaca gctttaagaa taattatgag 180 aaggctttgaagcagtataa ctctacagga gattatagaa gccatgcagt agacaagatc 240 caaaatacgttgcattgttg tggtgtcacc gattatagag attggacaga tactaattat 300 tactcagaaaaaggatttcc taagagttgc tgtaaacttg aagattgtac ctgccccggg 360 ccgnccgctcgaaagcttaa ntggccgttt cnaanncg 398 274 587 DNA Homo sapiens misc_feature(1)...(587) n = A,T,C or G 274 actttttttt tttttttttt tttgttgaatcaaaagcagg gtttattttt ctatcaaatc 60 cccaatccat gttccagcca atggatgaagggtgaatcaa gccccacata gactcttggt 120 aaaaacaatt ctaactttct aaaaaaaaaaaaagccaaca cacttttttc tttcttttca 180 aaaagctccc aggcctttgg gaacagctgaaacaaattca tatcctgact aggtctgttt 240 tctcttaggt atttggatgg tccctctctgctgccacttc tgcacagatg aggcactgat 300 aatggcctgc aggtcactca caatcctagctccacatcac tccatggttt gataacctag 360 aaccacgtta tgatttccat ttataatgccctaagaacag ctgaaaagat ctgtattaaa 420 ttctgcaaat ctttattgag tgccactatttgctgggcac angctaggcn ctggattctg 480 ctggttcttg agaaacctaa aanggnnccttnggccggaa cacccttang gcgaaatcca 540 cncactgggg ggcgtactaa ngggatccaactttggncca acttggg 587 275 588 DNA Homo sapiens misc_feature (1)...(588)n = A,T,C or G 275 actttttttt tttttttttt tttgccttta taagagaatttttattgtta attatttacc 60 ttaatagttt cagaaagagg aacaaattag ctcagtccaacatgattggc agttggcata 120 ttctagtgaa gcaagtgttc tgactgctaa ggatttaatttggataattt taatacttag 180 ccatctaaca cttcaagcat aacccagaat aaatgcaccaccttcctttc actttaatac 240 ccgnacctac ctcacttcga tataagaaat atcattcaatatgatttcca gaagggacaa 300 gtttcctgga gaatacaggc atganggaca atgcacaaaaagaaaaactc aaaatnaaac 360 tctggatgga taattactaa gctaagggaa ccaaaccttccaatttntaa agaaattaaa 420 tccggttcca aatgcctnat angnctatgt tnaaaaggttctggattaat accggaaaag 480 gnttgnttnt tacaggatnc cccaaccgtt acgggcccttngcccagaat gggccttaaa 540 anccaaagng tcttttccgn ngaggcccca tttnanaatccttntttt 588 276 595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,Cor G 276 actttagata catcattcct caaaaagttt ttaacggaga aagtggggcaattcaatggg 60 ggaaaggacg gcctttttaa caaatggtgc tggttctact gggtatctgcatccttgata 120 cacagaagtt aactcaagat ggaccacaga ctcacatgta agagctaaaataacattctt 180 agaagaaatc atggaagtaa atcttcgtga ccttggatca ggtaatgggtactttttttt 240 tttttttttt ttttttttta tcagattaat tttactttat ttcttcaggcctggggtttt 300 tcgatgactt caaatttggg atcttcaaat ttgaaggtgg gaaatggtattcatgtctgc 360 attaccaaac atttgctttg acttaaaaag ctcctctcca gctcttgccgatctctgaac 420 tagcatcaac aggntcctcc agatgtctgg nccttaaatt tggattccctaatcttggcc 480 acaaagangt ttcttggata gggaacaaag ttcccttatt naaatgccantngtngaacc 540 nccaatgttc cttcncaaaa ngggcttaaa ccggttaccc aattgacaaaggaaa 595 277 597 DNA Homo sapiens misc_feature (1)...(597) n = A,T,C orG 277 ggtactgttc ctgttggccg agtggagact ggtgttctca aacccggtat ggtggtcacc60 tttgctccag tcaacgttac aacggaagta aaatctgtcg aaatgcacca tgaagctttg 120agtgaagctc ttcctgggga caatgtgggc ttcaatgtca agaatgtgtc tgtcaaggat 180gttcgtcgtg gcaacgttgc tggtgacagc aaaaatgacc caccaatgga agcagctggc 240ttcactgctc aggtgattat cctgaaccat ccaggccaaa taagcgccgg ctatgcccct 300gtattggatt gccacacggc tcacattgca tgcaagtttg ctgagctgaa ggaaaagatt 360gatcgccgtt ctggtaaaaa gcttggaaga tggccctaaa ttcttgaagt ctggtgatgc 420tgccattggt tgatatggtt cctggcaagc ccatgtgtgt tgaaagcttc ttaaactatc 480cacctttggg tcgctttgct ggtccngatt tgagacanac catttccgnn gggtggcaat 540caaaccattg ggccaanaaa gnttntggac ttgcaagggn nccaaatttt ncccaaa 597 278595 DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 278ggtacttttt tttttttttt tttttttttt ttagtttatt aaaatactga gttttatttc 60acatgtatat ttttgtctcc ccaccatttc catgtctgac caccgctact actatgtcct 120atcataacat tccatacata cttaaaacca agcaaagggt ggagttccat ctttaaaaac 180taaacaggca ttttggacaa cacattcttg gcaatagaac ctggacaaca tttatcaaac 240acggtaggga aagttctcac tctgcattat aaaaaggaca gccagatatc aactgttaca 300gaaatgaaat aagacggaaa attttttaac aaattgntta aactattttc ttaaagagac 360ttcctccact gccagagatc ttgaatagcc tcttggncag tcattccgga aacaattctt 420ccataattga tgaatttggc tttcactttt gggaagagaa cccccttttc tatacttggg 480tgcattttgc ttaaaggctt ctacaaacta gggcctttgg gggtttaaga gttttccngg 540gtcttgaagg ntcttggcct ttgaacttgg ggtnaaaang gttgngcttt tccat 595 279 586DNA Homo sapiens misc_feature (1)...(586) n = A,T,C or G 279 ggtacgcggggagatacgtt cgtcagcttg ctcctttctg cccgtggacg ccgccgaaga 60 agcatcgttaaagtctctct tcaccctgcc gtcatgtcta agtcagagtc tcctaaagag 120 cccgaacagctgaggaagcc cttcattgga gggttgagct ttgaaacaac tgatgagagc 180 ctgaggagccattttgagca atggggaacg ctcacggact gtgtggtaat gagagatcca 240 aacaccaagcgctccagggg ctttgggttt gtcacatatg ccactgtgga ggaggtggat 300 gcggctatgaatgcaaggcc acacaaggtg gatggaagaa ttgtggaacc aaagagagct 360 gtctccagagaagattctca aagaccaggt gcccacttaa ctgtgaaaaa agatatttgg 420 tggtggcattnaagaagacc ttgaagaaca tcacctaaga gattattttg acagtatgga 480 aaattgaatgattgaaatca tgacttgacc aagcatggcc aaaaaagggc tttgctttga 540 accttgagacatgattcngg ataaaatgcn tcnaatncnt ntggga 586 280 612 DNA Homo sapiensmisc_feature (1)...(612) n = A,T,C or G 280 actttttttt ttttttttttttttttcttt tttttttttt tttttttttt ttttgaaaaa 60 gtcatgaagg ccatggggttggcttgaaac cagctttggg aggttcgatt ccttcctttt 120 ttgtctaaat tttatgtatacgggttcttc aaatgtgtgg tagggtgggg ggcatccata 180 tagccactcc aggtttatggagggttcttc tactattagg acttttcgct tnaaaacgaa 240 ggcttntcaa atcatgaaaattattaatat tactgctgtt anaaaaatga atgagcctac 300 anatgatagg atgtttcatggggngtatgc atcggggtaa tccnaataac gtcggggcat 360 tccggatagg cccaaaaangtttntgggaa aaaaagtttn atttaccccc attaaattta 420 tnnnnaaaag ggattttgcctaaggttggg ctaagggggt ancccngaaa attgggggaa 480 atcangnaat gaaaccccctntgatggnca aaaacagctc ctnttggttg ggccttatng 540 ggaanngggc ttcaacntannaccttnggc ggnaaaaccc ttanggngaa ttnnnnncaa 600 ntgggggggg tn 612 281593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 281acgctgcttc ttcagagcaa tacgccgccg tttgtgctgc aggacacgtg gagtaacaag 60acgctgaatc ttgggtgctt tggtcctagg tttcttacct tctttattta agggctttct 120tacaacatac tggcggacat catcttcttt agagagattg aaaagtttgc ggattctgct 180agctcttttg gggcccaggc ggcgaggcac tgtagtatca gtcagtccag gaataccctt 240ctctcctttt tttacaataa ccaagttgag aacgctcaga tttgcatcca caatgcaacc 300acgaactgat tttctctttc tttctcagtt ctccttggtc tgtaacagga atgcccctta 360ctcaatanca ggcggacacg ggcatgggtc aagacaccct gcttcatggg gaaaccttgg 420ttgncgttcc accactggat tcggaccaca taaacctttc attcttnaac caaacgtaac 480ancaactttt ggnggccata cncttttata naaagtccgg ggganaagtn ttttgcagga 540caagcctgta acnaatagtn aaatcccgga tttggattcc taancctttt ccn 593 282 595DNA Homo sapiens misc_feature (1)...(595) n = A,T,C or G 282 ggtacaattcaagaaactaa gtatttatgg gcattgaaga aaaaatgttg agataaaatt 60 gctgtgcagaaaaaagtgtt aatgaagccg acctgactac ttaaccttag agacctgctt 120 tacaaggttggcccttgatt ggcatctggg aacttggagt tcagggggct tccaccattc 180 ccagaactgatcaaagtagc ttactatatc taaactgtaa aacaatatag tttctcctga 240 acacctgctttccttctggg agtctggaat tttggtatgt gccaggcaga gactaccttt 300 gtgaccagctcccagtaaaa accccaggca ctcagtctct aacaagcttt tctggttgac 360 agtgtttcacaagtgctggt acaactggtt gctgggagaa ttaagctcat cctctgtgat 420 tccactggccgaggattctt ggaagcttgc acttaagttt cccctgactt caccccatgg 480 gcttttttccttgctgattt ggtttgnatc cttcctgnat aaatcatggc ctgaaccnaa 540 cttgaaaaaaaaannnnnnn nnaaaaaaag gtncttgccc ggcggccgtt naaat 595 283 348 DNA Homosapiens misc_feature (1)...(348) n = A,T,C or G 283 actttttttttttttttttt tttttttttt ctattttttt ttttttttgg ctntanaggg 60 ggtanagggggtgctatagg gtaaatacgg gccctatttc aaagattttt aggggaatta 120 attntaggacgatgggcatg aaactgtggt ttgctccaca natttcanag cattgaccgt 180 agtatacccccggtcgtgta gcggtgaaag tggtttggtt taaacgtccg ggaattgcat 240 ctgtttttaagcctaatgtg gggacagctc atgagtgcaa nacgtnttgt gatgtaatta 300 ttatacgaatgggggcttna atcgggagta cctnggccgn naccacnc 348 284 563 DNA Homo sapiensmisc_feature (1)...(563) n = A,T,C or G 284 ggtacccatt aatttgctcagatatagcag gcttaatggt tctatatttt caaaagtttt 60 taagaatggt ttctaacgtaggagagggaa aacatccacc atcccttttc agaatttaaa 120 tggagggcag taaacattctttacacccaa aacctatggc agcagttcaa atttgaccaa 180 ggtaaatgta gaatagagatgttctaaaca cagctaggac tcagcaagtc taacacacta 240 aaatcatatg attacattttaaaagaaaat gcacaaaaac caaatagaaa ttttgagatt 300 ttttttcatt tgaaggtaatcttaatgcta ttaaattcac aaatgctaat ttaaataccc 360 aatcctattt atctaaaacacacattgcaa acacacaaat tatctattct ctccacatgt 420 cagccgccca ttcatatcatggtttggaaa tgggggagaa atagattncc cttaaactgc 480 aagtcaacan ggggttctttacagttaact ttagccaaat tcataccaaa tacccgggta 540 cctgcccngg cggccgttcnaaa 563 285 422 DNA Homo sapiens 285 acaatggact ggatactaga aattttcttttcactcaaca gaacataggc atcctggaat 60 tcacatttct gaccttttga tgtattaataaagtatggag aaatatagcc tcgatcaaac 120 ttcatgcctt caataatttc taattcatcattcagtgttt ttccatcctt tactgtgatg 180 acaccctttc ttccaacttt tttcattgcatcagagatga tattgccaat ttctttgtct 240 ccgtttgcag aaatcgtagc aacctgtgcaatttcttcag gggtggtcac aggtttagac 300 tgctttttaa gttcagcaat tacagcatcaacagctaaca tcacacctct cctgatttcc 360 actggattag cacctttgct aatcttctcgaagccttctt ggctatagag cgtgccagta 420 cc 422 286 588 DNA Homo sapiensmisc_feature (1)...(588) n = A,T,C or G 286 actgttcctg caggttaaggcaggactgga actcctccac agcttgcaca tagttttcag 60 attcaacact aacttctccgagtttaagat gtgcctgggc agcataaagc tgtgcttctt 120 ttgtttcttg ccttttaaaaatgatctttg ctaaatccag catatcccag gcaagctcta 180 ggttcccaat ctcctcctcctcattttctt gaagagactt gttttcaagg actgaatcat 240 ttggcatttc ttcggtcttatcattttctt tatcatcctc ttctgagcct tcagtttcat 300 ctatgttatc attattttctaccagagatt catcttctgn tnttttctcc ttcttcctct 360 tncacatgca caccttccaaggcgtttcca acacaccatt cttcatcttg ccaacttcag 420 aagtggattt ccatagaaaaagaangnttn ttcacactta ttaactgctc ttcatacttt 480 ttacctnaaa gactaactgnttcctggaat gcattggccg ctgctnggaa atccccatan 540 cngaagttnt ggcctaanccaaagttntta gttactttcc catccgac 588 287 583 DNA Homo sapiens misc_feature(1)...(583) n = A,T,C or G 287 actggaactc caggaagcgc tggccagcctcatacgggag ccatttttct ttcactgcct 60 ctgctgctga catcttcttc tttcccttcacaccctcgaa gcctatgaag gctttctgag 120 caggcttcag cctggtggcc atgtcttggtcaatcacacc ctgggagact gcgtcctgaa 180 gtgacagctt ctggcccgtg gttgggtggatgatgccacc tgtgcaggcc tgagcctcca 240 gaagcctctg acccgtgatg ctgtcaacgatgccccgctc tataccttct gtaatggaga 300 ttttctccag gttttctgtg tcaaagatggctgcaatggg gctcgattct tncagggtgt 360 ctgaaaaaga actgctcctt atggntaaattcctgacctg gatatggtgg aaatcttact 420 tactgattca tgtcgggagc tgctaaaaacatnatcgttg caccactggc catgctgtgn 480 ttggngccac accatttttn angngacatgtaacnaattg antaggttag nttccgaacg 540 gaccttggcc ggaacaccta aggngatcanncatggggcg tnn 583 288 607 DNA Homo sapiens misc_feature (1)...(607) n =A,T,C or G 288 ggtacttttt tttttttttt tttttttgtt atttagtttt tatttcataatcataaactt 60 aactctgcaa tccagctagg catgggaggg aacaaggaaa acatggaacccaaagggaac 120 tgcagcgaga gcacaaagat tctaggatac tgcgagcaaa tggggtggaggggtgctctc 180 ctgagctaca gaaggaatga tctggtggtt aagataaaac acaagtcaaacttattcgag 240 ttgtccacag tcagcaatgg tgatcttctt gctggtcttg ccattcctggacccaaagcg 300 ctccatggcc ttcacaatat tcatgccttc tttcactttg ccaaacaccacatgcttgcc 360 atccaaccac tcagtcttgg cagtgcanat gaaaaactgg gaaccatttggggttgggtc 420 cagcattttg catggaccan aatgccagga cccctatgct ttaaggatgaanntcttatn 480 ttnaaatttc ttcccataaa nggcttgcca ccaangccat tatngcgngtgaagcaccac 540 ctgacccata accctggaat aattntnnga aaaccggacc cttntaccnaatcttttttc 600 aggggnn 607 289 591 DNA Homo sapiens misc_feature(1)...(591) n = A,T,C or G 289 actttttttt tttttttttt tttgagaatgaataagcagt tctttaatgg ttatttaaat 60 atattccaga agagcgttta taattcatttacaagtgcag tattgcgcta gtaaatgtta 120 cttgacctct tgtataaata atgccgattaagaattagtc ctggaatagt tttcgaattt 180 ctaactctgt agatctaaaa cacaattgtaaatggtataa agatgtaaga atcatattgt 240 gataaagtca atctcaaaaa tagagaatccagacccttcc cagataattt aagaactgag 300 ttttcctcaa cttaaacatg atggccacacagaaaacagt aaagacactt ttcgatgtga 360 tacaactgga taaaactcga gaatatgagtatttagngac caatgnatan acattantgg 420 aattttaaaa ncccttttaa tctgaagccgaaaaaaangc cattttccaa gaattattgn 480 gccctaatca tcatcnannc nngaatannatncnttcccn ggatagnnnn nnntccncct 540 tnggaaantg ggccnaantt ntttggtntnaaagggggnc cnttaantcc n 591 290 592 DNA Homo sapiens misc_feature(1)...(592) n = A,T,C or G 290 ggacttggaa atggttgtct ggaaagcttccactttggtc ttgacggcat tcaccctctc 60 cagcaccttc tcctggattg ctaccccaaaatcatttcca tcttcaatct tggggatcag 120 gtgttggatc catgtaatca ccagaatgcatttctctttg agagtccaga cttctggctt 180 aaccagggca agcagggaca ggactttctcattcccaggg agaaatccac acttagggac 240 ttctttcttc tcctgcttat ctgtttccatctcatcatcc ttgggtggag ggtctgggat 300 ggggatgtcc agtggggccc ggagggaagtcaagtcagcc acattgaggg agtcctcttg 360 caagagctga ttcaggtata tgattttctgtggcaagaat ctgtagagga attcctcanc 420 ctnctggaaa agaatctgtc tgaagaccttcacctggttg cgggctttcc cgctaagcgc 480 accccacacg gtttgggcct gctgntttaatccttaanct ctggcttccg gntagtcccc 540 cgggaccttg ccggccggcc ntcaaagggcaattcancna ctggcggccg tn 592 291 609 DNA Homo sapiens misc_feature(1)...(609) n = A,T,C or G 291 acagtggcat gatctcggct cactgcaacctctgcctccc gggttcaagc aattctcctg 60 cctcagccac ccaagtagct gggactacaggtgcgtgcca ccacgcccag ctaaattttg 120 tatttttagt ggagacgggg tttcaccatgttggccagga tggtctcaat ctcctgaccc 180 tgcgatctgc ccacctcagc ctcccaaagtgctgggatta caggcgtaag ccaccgggcc 240 tggcctgttt tatgattctt aatagttacttggtttaaat cacatttgat actatccttc 300 tgaaaagtct gagacagatc tacaaactacagtcaaaatt atagattaag aggaatgaat 360 gcacctattt ggctttaagt tgaagatgaattatttctca tgctcatttt cttgcngcag 420 ttatcttaga aagaccccca aaggcttgtgattgtaaagc acttgcatga tcacagaatg 480 caagcttctg gtaccttcgg ccgtgacacgctaagggcga attcatcaca attgcgggcc 540 gtacctatgg atccannctc ggtccaacttggcggaatca tgggcatact gnttcctggn 600 nnaaatgtn 609 292 568 DNA Homosapiens misc_feature (1)...(568) n = A,T,C or G 292 actgcccagaaggagttcat aaagaataca aagaagaccc caaaagatgt cacgatggca 60 ctattgaattcacgagcatc gatgcacaca atggtgtggc cccatcaaga cgtggtgatt 120 tggaaatacttggttattgc atgatccaat ggcttactgg ccatcttcct tgggaggata 180 atttgaaagatcctaaatat gttagagatt ccaaaattag atacagagaa aatattgcaa 240 gtttgatggacaaatgtttt cctgagaaaa acaaaccagg tgaaattgcc aaatacatgg 300 aaacagtgaaattactagac tacactgaaa aacctcttta tgaaaattta cgtgacattc 360 ttttgcaaggactaaaaact ataggaagta agggtgatgg caaaatggac ctcaatggtg 420 tggaaaatggangnttgaaa gccaaaacca tnnnnnaaaa ncttagggcg aattccannc 480 actggcggccgtnctaangg atccagcttg gncccaactt ggggtaatca tgggcataac 540 tggtncctggggaaaatggt ttcccnnn 568 293 603 DNA Homo sapiens misc_feature(1)...(603) n = A,T,C or G 293 ggtacttttt tttttttttt tttttttttttttttttctt tttttttttt tttttngcct 60 ttttaanaaa cttttatttg agnggntnttacaaanattg nngcaatatg aaagtcattt 120 gtttgatana aatatcaagc tgncttgtcaaacacnctga agtaacccaa aaatntnttt 180 caaagctcac anagcttaaa aagagcnaagattntntgca accagacaaa acctatttnt 240 gcatttccta tttctttctn aaactgntttgcctaccaaa ctttnacgtt taaacatttt 300 caggaaatgc agggatcatt ttgtttggaattttaagacc ccccngaacn cataggtntt 360 tacaaagaaa cttttcccga tcccttaattgaaaagaacc ntccnaaata taaantttgn 420 aaactcccnt ttttggccaa ttgatcanaatgccagaaga natgctaacc naanagccct 480 ttaactgggc tgggattcca taccctaaanggggtttcaa aactggttaa ccttnnccca 540 attttaacct tngggaaaag ggnaaagganccccggggna aaaataaggt tttgaaaaat 600 aaa 603 294 617 DNA Homo sapiensmisc_feature (1)...(617) n = A,T,C or G 294 ggtacgcggg gatcgcttcctggtcctcgc cccctccgct gtctccctgg agttcttgca 60 agtcggccag gatgtctcaggctgagtttg agaaagctgc agaggaggtt aggcacctta 120 agaccaagcc atcggatgaggagatgctgt tcatctatgg ccactacaaa caagcaactg 180 tgggcgacat aaatacagaacggcccggga tgttggactt cacgggcaag gccaagtggg 240 atgcctggaa tgagctgaaagggacttcca aggaagatgc catgaaagct tacatcaaca 300 aaagtagaag agctaaagaaaaaatacggg atatganaga ctggatttgg ttactgtgcc 360 atgtgtttat cctaaactgagacaatgcct tgtttttttc taataccgtg gatggtggga 420 attcgggaaa ataaccagttaaaccagcta ctcaaggctg ctcaccatac ggctctaaca 480 gattaggggc taaaacgattactgactttc cttgagtagt tttaatctga aatcaattaa 540 aagtggattt tgtaccaaaaaaaaaaaaaa aaaaagtnct gcccggccgg ccntcaaaag 600 gcnaattcan ccccttg 617295 606 DNA Homo sapiens misc_feature (1)...(606) n = A,T,C or G 295cgaggtactt ttaccatgaa catctctaga ctgtgattat taaatatagt gataatatac 60atgggtttac tgggatattg aaaaataaaa gataatgaac ccaatttagt aaatcaacat 120aaatacaaaa cagagcgaat tagccctcta caactgagct cgtcctgcgt cttgagcttg 180ggttctttct ggaactgtct caaaccttag tgggggaagt gaccttatcc acagattgct 240tttcccagag gttccgcttg ctggatacgt ctcctggtct caagtcagaa ggtttgggag 300caggtgactt gtttccatct ggggttttag ttagccattc attgatgccg ctagaaaccc 360ctaccttcaa gccagcagtt tccttatttg gtgtgcctgc tgcantgggg gatgaaaaca 420cattcctttc tnccacatac tcttggatgt tgcgtacctg cccnggcngg ccgttcnaaa 480ggccaattcc acaccactgg cggccgtact aatggatcca aaactcggac cancttggcg 540natcatnggc atactggttc ctggggnaaa tggattccgt tacattcccc caacttccag 600ccnggg 606 296 612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,Cor G 296 ggtacgcggg gtgccagagg aaatcttaaa gcgcctactt aaagaacagcacctctggga 60 tgtagacctg ttggattcaa aagtgatcga aattctggac agccaaactgaaatttacca 120 gtatgtccaa aacagtatgg cacctcatcc tgctcgagac tacgttgttttaagaacctg 180 gaggactaat ttacccaaag gagcctgtgc ccttttacta acctctgtggatcacgatcg 240 cgcacctgtg gtgggtgtga gggttaatgt gctcttgtcc aggtatttgattgaaccctg 300 tgggccagga aaatccaaac tcacctacat gtgcagagtt gacttaaggggccacatgcc 360 anaatggtcc cgcaggaagg ccgtcaagaa nggctcgacc cggntggtgtttcaaggaag 420 aaacattgtg gtcttggtgt ggaaaaaaaa tcantgggcc aactggnggatgaaagacna 480 tgccggaana nctgggcttt ggatgacaac ccctgcatgg gcttttgangccttaccgcc 540 gatccagggt tntnttaaca nggcccggtg gaatgccnaa nccccggttactttggagga 600 cccggtnctt gg 612 297 590 DNA Homo sapiens misc_feature(1)...(590) n = A,T,C or G 297 acgcggggga acacatccaa gcttaagacggtgaggtcag cttcacattc tcaggaactc 60 tccttctttg ggccacggaa ttaacccgagcaggcatgga ggcctctgct ctcacctcat 120 cagcagtgac cagtgtggcc aaagtggtcagggtggcctc tggctctgcc gtagttttgc 180 ccctggccag gattgctaca gttgtgattggaggagttgt ggctgtgccc atggtgctca 240 gtgccatggg cttcactgcg gcgggaatcgcctcgtcctc catagcagcc aagatgatgt 300 ccgcggcggc cattgccaat gggggtggaattgcctcggg caaccttgtg gctactctgc 360 agtcactggg aacaactgga ctcttcngattgaccaagtt catcctgggc ttcattgggt 420 ctgccattgc ggctgcattg cnaggtctactaacttcctg cccttgcctt gcaaaaaaaa 480 aaaccttgcc agggaaaaag nccccaanccttctgaacca accanggggc ccacttttcc 540 aaaatacctn gggnggaaaa tncccaattttgantttcnn aggaaanana 590 298 590 DNA Homo sapiens misc_feature(1)...(590) n = A,T,C or G 298 ggtactttga gccactctcg catggaaaggagtgtcttta tgcctcgacc tcaagctgtg 60 ggctcttcca attatgcttc caccagtgccggactgaagt atcctggaag tggggctgac 120 cttcctcctc cccaaagagc agctggagacagtggtgagg attcagacga cagtgattat 180 gaaaatttga ttgaccctac agagccttctaatagtgaat actcacattc aaaggattct 240 cgacccatgg cacatcccga cgaggaccccaggaacactc agacctccca gatttaacta 300 aacaaaagaa actctccacc tagcactgtttttcttcatt gcttactgag agggtttttg 360 agaacttaat ctggggggag aactgctttctcagatcctt aactcccgag aagagaagtc 420 cttgtgcaca gaacttgtgg gaaccttcatccgntgtctt tacctttgga tccagtgtgc 480 aagtttcatg acngaatcat taagatatcaaatggcctaa tttggngcna atcatggtat 540 actgggaaaa ttaggcnaat ggaacttntcaccgantttg gtctttaaan 590 299 549 DNA Homo sapiens misc_feature(1)...(549) n = A,T,C or G 299 cgaggtacaa agatctgaca tgtcacccagggacccattt cacccactgc tctgtttggc 60 cgccagtctt ttgtctctct cttcagcaatggtgaggcgg ataccctttc ctcggggaag 120 agaaatccat ggtttgttgc ccttgccaataacaaaaatg ttggaaagtc gagtggcaaa 180 gctgttgcca ttggcatcct tcacgtgaaccacgtcaaaa gatccagggt gcctctctct 240 gttggtgatc acaccaattc ttcctaggttagcacctcca gtcaccatac acaggttacc 300 agtgtcgaac ttgatgaaat cagtaatcttgccagtctct aaatcaatct gaatggtatc 360 attcaccttg atgaggggat cggggtaacggatggtgcgg gcatcatgag tcaccagatg 420 anggattcct tttgtgccca caaagatctttctactttgc ancacacact ggcggncgta 480 ctagtggatc cacttcgnac caacttggcgtatcatgggc tnactggtnc cgggggaaat 540 ggtatccnn 549 300 591 DNA Homosapiens misc_feature (1)...(591) n = A,T,C or G 300 actccagcctgggcgacaga gcaagactcc acctcaaaaa agaaatattt agcaaatatt 60 aaaggacaagagggaatatc tgtttaaaaa attataatgc acgttagatg aaaagtaata 120 ggatgagatggttgttgctg aaatagcact tgctatataa attcaaacat tccttttcaa 180 attcagcttctcagaggttt gacttcagat gcttgagcac tttcaacatt atctttgcct 240 ttatccttcnttatgcggat aaacacaact gctaaaatta taccattgat tttggaaact 300 tcccagtcgttttgtaagct tcactgccga gggaaaatgt aaaatgggga ccccgaaata 360 aagtgctgatcatcatcaag tagcctcgaa aatgagactt tcaggtgcac tgaaggggat 420 ggcagaagaacaagccccgt gtagtccttg ctagcctggg aaggttggca ttcacatcct 480 taaggatcangtggactttg acnccgaact taaaggaaga accccctatt ntggggccac 540 cacttgaccttgggccggaa cacccttaag gcgaattcca cacactgggg g 591 301 655 DNA Homosapiens misc_feature (1)...(655) n = A,T,C or G 301 cgaggtactctttaaaaagg gactgcaggg ctgggtgtag tggctcacac ctgtaatccc 60 agcactttgggaggccaagg caggtgggtc acttgaggcc aggagtttga gaccagcctg 120 accaacatggcaaaacccca tctctactaa aatacaaaaa ttagctgggc atgatggtgc 180 actcctgtaatcccagctac ttggtaggct gaagcatgag aattgcttaa acctgggagg 240 cagaggttgcagtaagccaa gatcatgcca ctgcactcca gcctgggcaa cagagtaaga 300 ctctgtcttaataaataaat aagaaaataa aacggaactg cagtgctaac agtaatttat 360 acatttttaaatgttctgag tatgttttga ctgggctagt gtaacaatat actaccctga 420 aaagtgcagttttgattgtt ggtggtgtct ttgggtcang aaaagtgaac tgtgccaaga 480 agtatttttcaatgacatga atggattnct gttaatgcaa ttgactgaga aaatgngctt 540 acgctttcttaactgcaaaa agagntttgt ccacatcana attgttgaaa ctggngctgt 600 ttctgttgcctgggatctga tgactgggat ttcctcttgg acaaaanacc tgatn 655 302 513 DNA Homosapiens misc_feature (1)...(513) n = A,T,C or G 302 actcgtcttggtgagagcgt gagctgctga gatttgggag tctgcgctag gcccgcttgg 60 agttctgagccgatggaaga gttcactcat gtttgcaccc gcggtgatgc gtgcttttcg 120 caagaacaagactctcggct atggagtccc catgttgatg gatcctgagc ttgaaaaaaa 180 actgaaagagaataaaatat ctttagagtc ggaatatgag aaaatcaaag actccaagtt 240 tgatgactggaagaatattc gaggacccag gccttgggaa gatcctgacc tcctccaagg 300 aagaaatccagaaagcctta agactaagac aacttgactc tgctgatttt tttttccttt 360 tttttttttaaataaaaata ctattaactg gacttcctaa tatatacttc tatcaagtgg 420 aaaggaaattccaggcccat ggaaacttgg atatgggtaa attgatgacc aataatcttc 480 acttaaagncatgtcctttg gccgcgaaca cgc 513 303 610 DNA Homo sapiens misc_feature(1)...(610) n = A,T,C or G 303 acgcggggct tgcagagccg gctccggaggagacgcacgc agctgacttt gtcttctccg 60 cacgactgtt acagaggtct ccagagccttctctctcctg tgcaaaatgg caactcttaa 120 ggaaaaactc attgcaccag ttgcggaagaagaggcanca gttccaaaca ataagatcac 180 tgtagtgggt gttggacaag tnggtatggngtgtgctatc agcattctgg gaaagtctct 240 ggctgatgaa cttgctcttg tggatgttttggaagataag cttaaaggag aaatgatgga 300 tctgcagcnt ggggagctta tttcttcanacaccttnaaa ttgtgggcag atnaagatta 360 ttctgtgacc cgtcaattct tanattngtagttggtnact gcatggaatt cngtcagcaa 420 gaaangggaa aantctngtt caatttggtngnataagaan tggttaatgg tcttcaaatt 480 cnttattcct tcagancggc caagtacctnggccgnganc atgcctaagg gctaattcna 540 ctcantggng gccgntctan ntggattccancttggtacc aancttggng ntattnatgt 600 caatanctgg 610 304 596 DNA Homosapiens misc_feature (1)...(596) n = A,T,C or G 304 ggtacctggaattatctaat tggccagagg tggcgcccga cccatcagtt cgaaatgtag 60 aagtaatagagttggcaaaa gaatggaccc cagcaggaaa agcaaagcaa gagaattctg 120 ctaagaagttttattctgaa tctgaggaag aggaggactc ttctgatagt agcagtgaca 180 gtgagagtgaatctggaaag tgaaaagtgg agaacaaggc cgaaagtggg ggaggaagga 240 gacagcaatgaggacagcag tgangactcc tncagtgagc angacagtga gagtggacgg 300 gagtcaggcctagaaaacan angaacagcc nagangaact caaaagccaa agggaaaaag 360 tgattctgaagatggggaga aggaaaatga aaaatctaaa acttcagatt cttcaaatga 420 cgaatctagttcaattanaa gacagttctt ccgattcttg aatcagaatc agaacctgaa 480 agtgaatcttgaatncngaa cagtcgctta ggagaaagaa agaaaccaag caggattgac 540 tccttttnccaagntgttcc ttctaaactg gatgatttaa ccngntccct cagtgn 596 305 629 DNA Homosapiens misc_feature (1)...(629) n = A,T,C or G 305 ggtactttnttttttttttt tttttttttt tttttttttt tggggattta ntttttattt 60 cataatcataaacttaactn tgcaatccan ctaggcatgg gagggaacaa ggaaaacatg 120 gaacccaaagggaactgcag cgagagcnca aanattntng gatactgcga gcaaatgggg 180 nggaggggngctntcctgag ctacaaaagg aatgatctgg tggntaaaat aaaacacaag 240 tcaaacttattnnagttgtc cacagncagc aatggngatc ttcttgctgg ncttgccatt 300 cctggacccaaagcgctcca tggcctccac aanattcatg ccttctttna ctttgccaaa 360 caccacatgcttgccatcca accactcant cttggnagng cagatgaaaa actgggaacc 420 atttttnttgggtccnacat ttccatggca aaangccang acccnttgct ttaagaagaa 480 aatctcatcttcaaattctn ccctaaanga cttgccncan gccntntggg tgngaagcnc 540 cccctgnccataaccctgga tatttttgaa agaggancct ntacnaacnt ttttccnggt 600 aanaaaaaattttttntttg acctnccca 629 306 643 DNA Homo sapiens misc_feature(1)...(643) n = A,T,C or G 306 acagggagga atttgaagta gatagaaaccgacctggatt actccggtct gaactcagat 60 cacgtaggac tttaatcgtt gaacaaacgaacctttaata gcggctgcac catcgggatg 120 tcctgatccc ccgcgtacat ttccttgtagactctgttaa tttcctgcag ctcctggttg 180 gttctggagc agatgatctc aatgagagagtcctcgtcgg ttcccagccc cttcatggaa 240 gcttttatct cagaagcgtc atactgagcaggtgtnttca ataggcccaa aatcaccgtc 300 tccaggtggc cagataaggc tgacttcaatgctgatgcaa gntccttttt ggtccttctc 360 tggtaggcga aggnaatatc ctgtctctgtncattgcttg cggntgggca aaatgttgac 420 aatggtgacc tcatccacac ctttggtctttgatggntgg ntcaatgttc aaagcatccg 480 ctcagcatca aaantaagta tangctttgcagacccatat gcacttgggg gngnngagng 540 acaccctcca actgaacttg ccaggatttntgaaagtaan anttttaaga acttgccgnc 600 cccanactaa acnnccaatc tagcccnntncctaacggcc aag 643 307 643 DNA Homo sapiens misc_feature (1)...(643) n =A,T,C or G 307 cgaggtactt tttttttttt ttttttttnt ttnttnttnn tttggggattnantttttat 60 ttcataatca taaacttaac tctgcaatcc aactaggcat gggagggaacaaggaaaaca 120 tggaacccaa agggaactgc ancgagagca caaanattct nggatactgcgancaaatgg 180 ggnggagggg tgctctcctn agctacaaaa ggaatgatct ggtggttaanataaaacaca 240 agtcaaactt attcnagttn tccacagnca gcaaagggga ncttcttgntgggcttgcca 300 ttcctggacc caaaacgctc catggnctcc caaaatttat gcctttttttactttgccaa 360 anaccacatg ctttgccttc caccnctcan tttttgnggg ggnaaataaaaancgggaac 420 cnnttgtgtt tggnccnaca ttttccnttg gnaaaaaacc ncgacccctttntttaagaa 480 naaaatttta nttttaaaat tttcccctaa aaaggactgg cccnaaggcnttttgggggn 540 gaagcccncc ntccccnaaa cctggaaaaa ttttggaagc nggaccctttaccaaatctt 600 tntcctggtt aaaaaaaaat tttttttttt gacctttccc aan 643 308653 DNA Homo sapiens misc_feature (1)...(653) n = A,T,C or G 308cgaggtacag agagtagctt ctgtgatgca agaatatact cagtcaggtg gtgttcgtcc 60atttggagtt tctttactta tttgtggttg gaatgaggga cgaccatatt tatttcagtc 120agatccatct ggagcttact ttgcctggaa agctacagca atgggaaaga actatgtgaa 180tgggaagact ttccttgaga aaagatataa tgaagatctg gaacttgaag atgccattca 240tacagccatc ttaaccctaa aggaaagctt tgaagggcaa atgacagagg ataacataga 300agttggaatc tgcaatgaag ctggatttag gaggcttact ccaactgaag ttaaggatta 360cttggctgcc atagcataac aatgaaagtg actgaaaaat ccagaatttc agataatcta 420tctacttaaa catgtttaaa agatggtttg tttgcaagac tttttgcata cttanttcta 480catgaattaa atcactggtt tnaaatgaca cttattaatc ctaataactg gtnaacccnc 540aaaaaaaaaa aaaaaaaaaa ntacttnccc ggcggccgtc gaanggcaat tcacncctgg 600cggccgtcta tggatccacc cggnccacct gggnaacagg cnactggttc tgg 653 309 649DNA Homo sapiens misc_feature (1)...(649) n = A,T,C or G 309 acttgcaaaagcacttgaag tcattaaacc agctcatata ctgcaagaga aagaagaaca 60 gcatcagttggctgtcactg cataccttaa aaattcacga aaagagcacc agcggatcct 120 ggctcgccgccagacaattg aggagagaaa agagcgcctt gagagtctga atattcagcg 180 tgagaaagaagaattggaac agagggaagc tgaactccan aaagtgcgga aggctgagga 240 agagaggctgcgccaggaag caaaggagag agagaaggag cgtatcttac aggaacatga 300 acaaatcaaaaagaaaactg tccgagagcg tttggagcag atcaagaaaa cagaactggg 360 tgccaaagcattcaaagata ttgatattga agaccttgag gaaatggatc cagattttat 420 catggctnaacagggtgaac aactggagaa agaaaagaaa gaacttcaga acccttaaga 480 atcagaaaagaagattgctn ttttgaagac ccacctttgg aaaaattcct ttgttaagag 540 cctttcgaggacagaaaatt aagacatggt ctggggngcc cccgaggaga aagaattctc 600 ctgcccttgacgtgaaaggt nttgcataaa atcatgtccn atcttgaga 649 310 319 DNA Homo sapiens310 cgaggtacta gccggacttg gattttctgg aaagatttca gttgaggaac gggaacaaag 60attatgatag ctttccgacc accaccaact tcaatttcct tagctgccgt aatattcagc 120tccctgagct gagccttgag gtccgagttc atctccagct ccagaagagc ctgggagatg 180ccggactcga actcgtccgg cttctcgcca ttgggcttca cgatcttggc gctcgaactg 240aacatggctt tctcctggga gaacttgccg agcgccggct taggaagaga ccccgcgtac 300ctgccgggcg ggcgctcga 319 311 646 DNA Homo sapiens misc_feature(1)...(646) n = A,T,C or G 311 cgaggtactg atgcaacagt tgggtagccaatctgcagac agacactggc aacattgcgg 60 acaccctcca ggaagcgaga atgcagagtttcctctgtga tatcaagcac ttcggggttg 120 tagatgctgc cattgtcgaa cacctgctggatgaccagcc caaaggagaa gggggagatg 180 ttgagcatgt tcagcagcgt ggcttcgctggctcccactt tgtctccagt cttgatcaag 240 ctgcacatca ctcangattt caatggtgcccctggagatt ttagtggtga tacctaaagc 300 ctggaaaaaa ggaggtcttn tntggccccaaaccaatgtt ctgggctggc caatgacttc 360 acatggggca atggcaccaa caccggcagaacttgnaccc tattgccaca acatgtcctt 420 atctnaatga nggncttctt tgtgaaaacaaaccccattc cccggaatta agnacaantt 480 cttcaaactt gggtggnttc aagggcctcgatngcctgcc catatngggt ttttgccata 540 aaacacaacn ttccnnaaag gaatccgantnttgttttgt tggancccat ttttgttccc 600 aagaaaattn ggtaatatcc aaattggggaattaggaaaa tgggnt 646 312 622 DNA Homo sapiens misc_feature (1)...(622)n = A,T,C or G 312 cgaggtactt ttgtgagagg gttcaatggg agagctttaatgcagatgag acttgaagct 60 tctgaagaag atctaagtct tgatgaggtt attcaaactcaaatcttgaa tgcataatga 120 tgataggcca tggtcttcaa aaacgtggta cttttaatagcaacagggtt tcaccatgtt 180 ggccaggctg gtctcaaatt cctgacctca agtgatctgcccacttaagt gctgggatta 240 caggcatgag ccacaacatc tggccagaaa tattttttcttttctttctc tttctctctc 300 tctttttttt tttttttttt tttggagctc gctctgtcccccagctgcaa tgcaatgggg 360 caatcttaac ttactgnaac ctccccttcc aggtcnaaagaatctttgng ctacctccta 420 natntnggaa tacaagggcg tcccccacct actaattttgntttttaaga aaaggagggt 480 ttancatgtt ggtnngntga tcccaacctc cgaccttaangancctccgc ctaatttcca 540 aaggctggat nttggctgan cccaccccnc ttaaccaaaattnaaattct tttntcctgc 600 cgggggcgtt aaagggaatc aa 622 313 674 DNA Homosapiens misc_feature (1)...(674) n = A,T,C or G 313 nggacttgaaatcattgaag ttctgcaaaa aggagatgga natgcacaca gaaagaaaga 60 tacagaggtccgcagacggg agctcctaga atccatttct ccagctttgt taanctacct 120 gcntgaacacgcccaagacg tggtgctaga taagtcagcg tgtgngtagg tntctgncat 180 tccngggaacagacnaattn gaccatnagg naacctgagc ttnccaaagt ncgcaaggct 240 gaagaaganaggctnctcca ggaagccnac gagaaagana aangagccgt attttacncg 300 aacatgaacaaatcaaaaaa naaaactgtc cgaaaaccgt ttggagcaaa ncaaanaaaa 360 cagnacctgggngcccaaag cattcnaana tatttgttat tancncaccn tgatggattc 420 naaacnttatttttncttgg cncggctggn ccgcccggct ngngnaaaga aaagaacttt 480 nctaccnctcccgaatcaag aaaagaanat ggcttttttn taaaanncaa cccttgggaa 540 aaaattctttgtttaananc cctccaangc ccgggaaatt aattcatgct ttgtgtgngc 600 gaccnannaaaaaanaanan atccttcctt ccccttaann gaaaagggcc ttncaaaaaa 660 tgattgcccaagnc 674 314 646 DNA Homo sapiens misc_feature (1)...(646) n = A,T,C orG 314 actttttttt tttttttttt tttttttttt tttgagatgg agtcttgctc tgtcgcccag60 gctggagtgc agtgttgcga tctcagctca ttgcaacctc tgcctcccag gttcaagtga 120ttctcctgcc tnagcctcct gagtagttgg gactacaggc acatgccacc atgcctggct 180aatttttttg catttttaag tanagacagg gtttcatcat gttggccagg caggtntcaa 240actcctgacc tcaagtgatc cacctgtctc agcctcccaa agtgctggga ttacaggcat 300gagccactgn acccggccta aaaatgatta cttcttataa aaaggatttc ttccccttca 360caacacttan cttccttttt ctttcctggn aactatgggt ntggngnccg cataaggatc 420taccttncnc aagctggaca ntgggggttg ctncttgang gnaactcagg ccanatacng 480accctggggg gaacnctaaa cttacttggg tanaacccgg gctaacattt ctgcttgnga 540ngttgattcc ccncaaattt ttaaaaggnn tttcatggcc cttagggcaa ccattttaca 600aagatgggnc acatggnctt ggccgnaacc cctangngaa ttcncn 646 315 666 DNA Homosapiens misc_feature (1)...(666) n = A,T,C or G 315 acagtctttggatatttagg aaggggatgg ggagaaagtc agttctcaga acaaattagt 60 cagcttcagtctcgtcagca gggtctttgg attctttgtt cttccgcact tcttcaatgt 120 gcttatccttctctcgcaaa cgttccagtt tggcagccat ttgtgcctct cggttctctt 180 tattagcttccattttgtgg gtcagtttct cttctgccat tttactgaag ttgntgttct 240 cttctattgccttctgaagc acttctttct cgtgctctcg tttctcancc agctgcttca 300 agaccttagcttcatgggac ttgcgtcttt cttctgcagc ttctaatttc ttctgaattt 360 cctccagggaaagaccttct tctttggaag ggaaaggggg aattctggaa ccagattctt 420 ttgacccaaggctgaaaatc agcttaaaag cctggccttg angcacccnt tttcagntct 480 ttcacctggatatcntaaag aagccctngt gattnaaaac aagccnaccg gcantnnatt 540 ntgncaanancnnggataan gnaatccctg tnaantccna cccctnaccc cattttcccg 600 ggaccttggcngnaacccct tanggngaat tcnnccnctn ggcggccgta ctaangggac 660 ccaccg 666316 656 DNA Homo sapiens misc_feature (1)...(656) n = A,T,C or G 316actcttggtt tgtcaatggg actttccagc aatccaccca agagctcttt atccccaaca 60tcactgtgaa taatagtgga tcctatacgt gccaagccca taactcagac actggcctca 120ataggaccac agtcacgacg atcacagtct atgcagagcc acccaaaccc ttcatcacca 180gcaacaactc caaccccgtg gaggatgagg atgctgtagc cttaacctgt gaacctgaga 240ttcagaacac aacctactgt gggtgggtaa ataatcagag ccttcccgnc aagtcccagg 300cttgcagctg gccnatgacc aacaggaccc tnactctact tagtgtcaca aggaatgatg 360ganggaccct atgaagtgtg gaaaccagaa ccaattaagt ggtgnccaca cganccaggc 420attcttgaat ggcccttatg gnccanaaga acccaccatt tcccctnata cacctaatnc 480cgtccagggt gaaccttaag ctntctggca tgcaancctt aaccactggc aggattcttg 540gnttaatgaa gggaacattc nnacccnccc agaagttttt attttcaact tacttggaan 600aacgggggct ntttactgcc ngccataact taacnggggc cnnancggac ttcgnn 656 317636 DNA Homo sapiens misc_feature (1)...(636) n = A,T,C or G 317actttttttt tttttttttt tttttttttt tttgnagtca gctatttaat taggttctta 60agacatttag aacaccaatt tgngaggata aattccattc gtcagagcaa acacagatcg 120caggtagccc tggagctgag gaatagcttt gatttttggt aaaatttgtg agtccacagc 180tttctgatca atcttgcgct gctccgtaat ctcatatttc cctttttctg ggncgaaaan 240cttacctttc tggggnntgg gcttncgcag cttcttcttn ttgaagtaag catnagtaan 300aagntttggg anttttacan tgntgatann cattttggnt gaagnggnan tgacnaattt 360ctgggggggt cttcgtaaag gaactcnant gaggcccaag ggnccgtccn agtaataagg 420ccctnncanc tggttangga aaccccctnt tggcctgggg ggnccangag gntgatccaa 480atggccccgg ggaaaagcng gntcaanttt tnacggctnc tnaaagggtt ttgccnggnt 540taanctttnn ggncnttttc agnggaaana ccngctttgn nantntaccc ccggntcctc 600ggcggaaacc nttagggnna attncncnct gggggg 636 318 654 DNA Homo sapiensmisc_feature (1)...(654) n = A,T,C or G 318 cgaggtacgc ggggcctttctgcccgtgga cgccgccgaa gaagcatcgt taaagtctct 60 cttcaccctg ccgtcatgtctaagtcagag tctcctaaag agcccgaaca gctgaggaag 120 ctcttcattg gagggttgagctttgaaaca actgatgaga gcctgaggag ccattttgag 180 caatggggaa cgctcacggactgtgtggta atgagagatc caaacaccaa gcgctncagg 240 ggctttgggt ttgtcacatatgccactgtg gaagaggtgg atgcagctat gaatgcaagg 300 ncacacaagg tggatggaagaattgtggaa ccaaagaaaa ctgtcttcag agaagattct 360 taaagaccan gtgcccacttaactgtgaaa aagatatttg gtggtggcat taaagaagac 420 actgaagaac atcactaagagantattttg aacagtatgg anaaaattgn agngattgaa 480 atnatgactg ccnangcagtggcancaaan ggggctttgg ctttnnacct ttgacnacca 540 tgactcnngg ataaaatggnattcnnaaat ccctcntgng aatggccnca ctgggaagtt 600 ngaaancctn ncaacnagaaagggtncgnt tnntccncca aangcnaang tttc 654 319 659 DNA Homo sapiensmisc_feature (1)...(659) n = A,T,C or G 319 acgcggggaa gccaactcagactcagccaa cagagattgt tgatttgcct cttaagcaag 60 agattcattg cagctcagcatggctcagac cagctcatac ttcatgctga tctcctgcct 120 gatgtttctg tctcagagccaaggccaaga ggcccagaca gagttgcccc aggcccggat 180 cagctgccca gaaggcaccaatgcctatcg ctcctactgc tactacttta atgaaagacc 240 gtgagacctg ggttgatgcaanatctctat tgncagaaca tgaattnngg caacctggtg 300 tctgtgctna cccangcccaaggtgccctt ggggcctcac tgattaanga aantggcact 360 gatgacttca atggctggaatggccttcat gaccccnaaa aagaacccgc gnttgcactg 420 gacagtgggt ccctngntctcttacaagtc tggggcaatt gganccccaa nccatgntaa 480 ttcnggctac tggggtgagcnnacctcagc ccaggatttn gaantggaan gcctgncttg 540 ggaanacaag ttcttctttngctngcaagt tcaaaaccta atgcagctgg aaaatcatnt 600 ctanaactga tcagcattcnaccgnttcaa attaaccggc ctttttcant tanttaccg 659 320 664 DNA Homo sapiensmisc_feature (1)...(664) n = A,T,C or G 320 ggtactctgc cttttaggagatgaggtaag acatatacat agatggcttt tactagccaa 60 ggcaatgtaa atggactaagattctcatgt gacttgaggt tatctgatga atttattctc 120 ttcaaaacca cctacttttagagggcatgt ttaacccctc tctttattta aggagggaga 180 gaaaaacaca tgtaaccagaattcagagtg ggttactcaa cctaagagaa catacggagt 240 tctctttggg aaaacgacaagactacagtg ttcacttcgc accatgaagt ggcactcctg 300 ntatggctgc agantcctcttacttcttat gaaaggatgc atctgattct gaaattactg 360 atatattcga tcagttagggatgntttaaa aagngaaaac caatgccaca catacacttt 420 ctagctttct gaaaatnacccgacacattn ccnaaaatng agaatttacc ctattacttt 480 tagagaaatt tccataatattcttgggtaa agaancccng ttgggcatat tnccaatttt 540 cagnggncnt ggttttatgcccnaganccc aataggntcc cccatttttt aaggcttttt 600 ccacngacga ttttttaaancnttctnnan tgggggaaga ataatcttaa aagtngnctt 660 atnt 664 321 666 DNAHomo sapiens misc_feature (1)...(666) n = A,T,C or G 321 cgaggtacagtattacagtc agccacagaa gctgtgttgg gggacaagac ccaatccttc 60 cccacaccaggcaaagcagt attggacatg agttggcatg tggctgggcc cacgtcctta 120 tcccccaggcctgaggggag accaccttct gatgataacc aacccctagc taccactctg 180 tattcatcaggggaggggta taaaccccgc atgcaagaag aacccttgcc cccagtgtca 240 aatgggatggggatgctaga gttatagtaa aggggaaacc ctatgtaagc tgntaacaga 300 gttcacaggggtagggataa cccctgntct tcagctncca aatgngctca ctttccagct 360 tcttcatccgtcatcaatgc tggcaaagtt tccctnaact gnggccaggt tttcacgcat 420 gggtggctgcacctgggtca aaaaggtggn attggccntt aaggaattag caatcatntg 480 ctgggtgggattccagtgtg taaggaactt anccaactgc atggnttgnt tgtgcanctg 540 cttgatggngacaagtttnt gcaccanctn aaggaaggtg gaagcatggg gctcaacctn 600 gataagttcatatacttggg gcnccttgct ttgggatctg catntttaca aggnttatcn 660 tggcan 666322 631 DNA Homo sapiens misc_feature (1)...(631) n = A,T,C or G 322accggaaagg aagctcccat tcaaaggaaa tttatcttaa gatactgtaa atgatactaa 60ttttttgtcc atttgaaata tataagttgt gctataacaa atcatcctgt caagtgtaac 120cactgtccac gtagttgaac ttctgggatc aagaaagtct atttaaattg attcccatca 180taactggtgg ggtacatcta actcaactgt gaaaagacac atcacacaat caccttgctg 240ctgattacac ggcctggggt ctctgccttc tccccttacc cttccggctc cacccttcct 300gcaacaacag ccctntacct ggggggcttg ntagaagaga tgtgaagggt tcaaggtcgc 360aacctgtggg actactgcta ggtgtgtggg gnggttcgcc tgcacccctg ggttctttaa 420gncttaaagt gatgcccctt tccaaccatt attctggncc cacacttctc actccggcct 480tggncnanca taaatgnacc ccttcacttc ctntgagaat ggccttcgng aagaatcnag 540gctttcccaa ncttctttcc ccccnttatc angggngctg gttttctnct ctcnaaggtc 600ntttgaccgn accaccaaac ttctgaattn t 631 323 647 DNA Homo sapiensmisc_feature (1)...(647) n = A,T,C or G 323 actgtgggtc gaagtaatggatacggacgt aaccatcttc gccgccgctg ctgtagctct 60 tgccatcagg atggaaggcaacactgttga taggtccaaa gtgacccttg actcttccaa 120 actcttcttc aaaggccaaatggaagaacc tggcctcaaa cttgccaatc ctggtggagg 180 ttgtggttac atccatggcttcctgaccac cgcccaggac cacatggtca tagttggggg 240 agagggcagc tgagttgacaggacgttctg tccggaaagt cttctgatgt tcaagagttg 300 tggagtcgaa aagcttggctgtgttgtcct tggacgcggt cacaaacatg ggcatgtccc 360 tggataactg gatgtccgtgatctgcccgg agtgcttctt aacattncca acacctnttc 420 aaanttggca ctatactgggtgagctcttc acttttatng gcaacgnatg atcacttccc 480 caagggtccc caaacagcactggggaattt agagncattc cagggaactt tatgtagggt 540 tcatggtgca attggttngatccccaggtc aaaaagttnc aaacactgga nccctttctt 600 gtccnnggag aacatgttatttgccccaag taaaacccng nccggng 647 324 653 DNA Homo sapiens misc_feature(1)...(653) n = A,T,C or G 324 ggtacttttt tttttttttt ttttttttttttgagatgga gtcttgctct gttgccagac 60 cggagtgcag tggtgcgatc tgggctcactgcaatctcca cctcccgggt tcaagcgatt 120 ctcctgcctc agcctcccga gtaactgggactacaggtgt gcgccaccaa gcccagctca 180 tttttgtatt tttagtanag atggggtttcacgatgttgg ctaggatggt ctcgatctct 240 ggtcagagtc ttttctgtaa aaatccttggtaaagaagca attttagact gtancctgtt 300 gcaaatgcnt taaggaagaa gcaaaacaactgntagtctt tctgaaatga aaaaactacn 360 ccagggctgg tatatnnaga gcaaccccaaccannactnc catcntgatg cccacagggg 420 cccactgana nacccngaaa angtccnnaagcntaaannt ngangcnttg cttttgaaat 480 attgcgccng taccnagntn nagacaaacnngnttaaggc ccnnantntt tggccngant 540 ttgcgataaa aaaaacttgg gggtcgctncnngatcccnn ttgtncccca naanctgggg 600 ggatgggttn aagcccntgn cnnaaggtttnngttctccc aaggtaaaan nng 653 325 655 DNA Homo sapiens misc_feature(1)...(655) n = A,T,C or G 325 ggtacgcggg gccttttggc tctctgaccagcaccatggc ggttggcaag aacaagcgcc 60 ttacgaaagg cggcaaaaag ggagccaagaagaaagtggt tgatccattt tctaagaaag 120 attggtatga tgtgaaagca cctgctatgttcaatataag aaatattgga aagacgctcg 180 tcaccaggac ccaaggaacc aaaattgcatctgatggtct caaggggtcg tgtgtttgaa 240 agtgagtctt gctgatttgc agaatgatgaagttgcattt agaaaattca agctgattac 300 tgaagatgtt caagggtaaa aactgnctgactaacttcca tggcatggat cttacccgtg 360 acaaaatgtg gtccatgggc aaaaaatggcagaccatgat tgaagcttac ggtgatgtca 420 agactaccga atgggtactt gcttcgtctggtctggggtg ggtttactaa aaaacgcaca 480 atnanatacc gaagaactct tatgcttangaccacangtc cngccaatcc ggagaanata 540 tggaaatctg accccaaagn gccnaccaatgacttgaaaa annggccatt aaatggttcn 600 nacacnttgg aaaagcctta aaaggttgccaantattaac ctntcatgaa gnttc 655 326 657 DNA Homo sapiens misc_feature(1)...(657) n = A,T,C or G 326 ggtacgcggg ggaaacggga gtgaacggagagcgtagtga ccatcatgag cctcctcaac 60 aagcccaaga gtgagatgac cccagaggagctgcagaagc gagaggagga ggaatttaac 120 accggtccac tctctgtgct cacacagtcagtcaagaaca atacccaagt gctcatcaac 180 tgccgcaaca ataagaaact cctgggccgcgtgaaggcct tcgataggca ctgcaacatg 240 gtgctggaga acgtgaagga gatgtggactgaggtacttt tttttttttt ttnttctttt 300 ttttgagata gggnctcact gnatnacccantntggaatg caattggcat gaacncagct 360 tactgnagnc ttccaaacct gggctcaagcaattatnttg nattaacctn ttgagtacct 420 gggactntcn cangcaccan ccctgctttgcttacttaaa tttttgtnaa nacnnggctt 480 gctttttttc ccaggntggn tcnaactccngaattaaggg atccttcccc ctcaattttt 540 aaannngctg ngattntnga atangcctttttgttngccc ttttnacctt ttnnnnggtt 600 nnttcnnggc tttaancctn ccgggggccntttaaaggng aaatcncncc ttggggg 657 327 658 DNA Homo sapiens misc_feature(1)...(658) n = A,T,C or G 327 ggtacttttt tttttttttt ttttttttttttttttttgg tttgaaacag aaatttattc 60 tcanaataat gcacagaagc acaggttgaggctactcttg ggaagcttcc ctccccttcc 120 tcttcctcct ctccctcctc tctgaatgccagggagaaca cagttgaagg aaggaaacat 180 gcaatcacaa acaatgaaca actntaaagacaaaaaggtt tggtccaaaa gaactcaaca 240 taattaatcc aatgactgtg aanagcttcactgagtagga ttaanatatt gcagatgtan 300 ngtttncaca gggtggctnt tcagtgcaccancggggcct ncttgangga natgaggact 360 gacncatncg ggaaanatct ttggcctgcttgctaaactt ggggntaaag gcacacnnnc 420 cgggccaccc gttccactna nngcctggggaccanttgtc aatgncnttt ccnaangntt 480 tttttgntgc cttgtggttg nttttggtttctggaactgn tcgncctgnc ttgnaaacca 540 ttnttntaac nccttaatgg cctttcttttcnnnctggtt ntgnttccaa aatnggatta 600 ngggttcang ngcccctact tnccgggggcngttaaangg naattccncc nctggngg 658 328 644 DNA Homo sapiens misc_feature(1)...(644) n = A,T,C or G 328 acgcggacgg tggtttttgg gcccgtttctgagcagcgct tcctttttgt ccgacatctt 60 gacgaggctg cggtgtctgc tgctattctccgagcttcgc aatgccgcct aaggacgaca 120 agaagaagaa ggacgctgga aagtcggccaagaaagacaa agacccagtg aacaaatccg 180 ggggcaaggc caaaaagaag aagtggtccaaaggcaaagt tcgggacaag ctcaataact 240 tagtcttgnt tgacaaagct acctatgataaactctgtaa ggaagttccc aactataaac 300 ttataacccc agctgtggnc tcttgagagactgaagattc naggctncct ggccagggca 360 gccctttagg agcttcttag taaaggacttatnaactggt tttnaancac agacctcaag 420 taattnacac cagaaatncc nnggtggagaatnctccnct gctggtnnag angcatgaat 480 aggnncaacc agctntctct gnccnnaccncncttaggnc naattccgca ccctgcggcc 540 gttctnatgg atccnaactn ggtnccaantnggcnnacta tggcatancn tgccctgggg 600 aantggttcc nttccaatcc anaanttctatcgnaactta acgg 644 329 651 DNA Homo sapiens misc_feature (1)...(651) n= A,T,C or G 329 actattagcc atggtcaacc ccaccgtgtt cttcgacatt gccgtcgacggcgagccctt 60 gggccgcgtc tcctttgagc tgtttgcaga caaggtccca aagacagcagaaaattttcg 120 tgctctgagc actggagaga aaggatttgg ttataagggt tcctgctttcacagaattat 180 tccagggttt atgtgtcagg gtggtgactt cacacgccat aatggcactggtggcaagtc 240 catctatggg gagaaatttg aagatgagaa cttcatccta aagcatacgggtcctggcat 300 cttgtccatg gcaaatgctg gacccaacac aaatggttcc cagtttttcatctgcactgc 360 caagactgag tggttggatg gcaagcatgt ggtgtttggc aaagtgaaaagaaagggcat 420 gaatattgtg gaggccatgg aaccgctttg ggtccaggaa tgncnagaacagcaagaaga 480 acaccattgc tgactgngga caactcgaat aagttggact tggggttantttaaccacca 540 gaacaattcc tttgtncnta aggagancan ccctcaccca tttgntngcatatcctanaa 600 actttgggct ttcnttngtt cctttggttc aggtttcctg gtcctccanc c651 330 643 DNA Homo sapiens misc_feature (1)...(643) n = A,T,C or G 330actttntttt ttttntnttt tttttttttt ctggaaggnt ctcaggtctt tatttgctnt 60ctcaaattcc aggaatngac ttatttaatt aatccatcaa cctctcatag caaatatttg 120agaaaacaaa tttatattca gattcttatt ttcagtaggg aagtaagaag ttgcagctca 180ttgcacgtaa agttgagaca ganatggaga catccagccc cacctntctg gaacaagaaa 240gatgactggg gaggaaacac aggtcancat gggaacaggg gttacagtgg acacaagggn 300gggctgnctn ttcacctnct tacattatgc taacagggac ncaaacccat tcaggggcct 360ttgcnaaaag aaatgccaaa agctnttgaa gtcncnaagg ggangcgtga anaaaactgc 420atttnagtcc ccgggtcctt ngncgggaac ccctnanggn gaaatcncca cactggcggg 480ccgtactagn ggatccagct nggncccaat tggnggaata tggnnaanac tgttcctgtg 540ggaaaatggn atccgtccaa ttcnccactt acanncggag cctaaangna aaacntgggg 600ngcctatggg gggctacnnn aataatgggt gcctacggcc cnt 643 331 652 DNA Homosapiens misc_feature (1)...(652) n = A,T,C or G 331 ggtacagatggcactgacaa tcccctttct ggtggggatc agtatcagaa catcacagtg 60 cacagacatctgatgctacc agattttgat ttgctggagg acactgaaag caaaatccaa 120 ccaggttctcaacaggctga cttcctggat gcactaatcg tgagcatgga tgtgattcaa 180 catgaaacaataggaaagaa gtttgagaag aggcatattg aaatattcac tgacctcagc 240 agcccgattcagcaaaagtc agctggatat tataattcat agcttgaaga aatgtgacat 300 ctccctgcaattcttcttgc ctttctcact tggcaaggaa gaaggaagtg gggacagang 360 agatggccctttcgcttang tggccatggg ccttnctttt cactaaaagg aattaccgaa 420 cagcanaaagaaagncttga gatagtgaaa atggggatga tatctttaga agggngaaga 480 tggggtggatgaaattattc attcctgnga agnttgnaaa ctgngcgnct tcnnnaaant 540 nnnaggcattccntnnctgg ccntgccatt gccattggnt ccanttgcta tagggatgcc 600 ccttaaancnntttccnnna anagtnnaaa acttgcnntn ggatccaacc nn 652 332 648 DNA Homosapiens misc_feature (1)...(648) n = A,T,C or G 332 cgaggtactttttttttttt tttttttttt tttttttgag acagagtttc actcttgtcg 60 cccaggctggagtgcagtgg cgcgatctcg gtccactgca acctcaccct cccaggatca 120 agcgattctcctgcctcagc cacctgagta gctgggatta caggcgcctg ccactacacc 180 tagccaatttttgtattttt agaagggaca gcatttcacc atgttggcca ggctggtctc 240 gaactcctgatctcaggnga tccacccacc tcagcctccc aaagtggngg gattacaggc 300 gtgagccactgaaagttctc attagttttt tggttaaatt ttaaacataa attatgttat 360 agcaaaaattcctaagaatt gnaaaccact ttatcagaaa tatcnnaaat tcacaaataa 420 tnccaaaatttataatagct tttttccaga ctaaaatttt aaagctactg anaagnggna 480 aacctncctanataggattt acctaacatt nnggantaaa aggnanccan ngcctgctaa 540 anatccagantatctaanaa tccntncctg nntctcnntc tatnttttca natccgaatt 600 tttgaacccacnttangata nctnntttcc cccttaacnn taattccc 648 333 656 DNA Homo sapiensmisc_feature (1)...(656) n = A,T,C or G 333 cgaggtacaa gatgtccaaatattgcgaag atctatttgg ggatctcctg ttgaaacaag 60 cacttgaatc acatccacttgaaccaggca gggctttgcc atcccccaat gacctcaaaa 120 gaaaaatact cataaaaaacaagcggctga aacctgaagt tgaaaaaaac agctggaagc 180 tttgagaagc atgatggaagctggagaatc tgcctcccca gcaaacatct tagaggacga 240 taatgaagag gagatcgaaagtgctgacca agaggaggaa gctcaccccg aattcaaatt 300 tggaaatgaa ctttctgctgatgacttggg tcacaaggaa gctgttgcaa atagcgtcaa 360 gaaggcttca gatgaccttgaacatgaaaa caacaaaaag ggcctggtca ctgtagaaga 420 tgagcangcg tggatggcatcttataaata tgtaggtgct ccactaatat ccatncatat 480 ttgtcaccat gatcaactaccccagnctgt naaggttcaa ggtttcatgt ggcanaagaa 540 ccccatattc ttttacatggcttctttaat gaatcatcgg cttggtactg aaaccctgcc 600 attgaattgc attntacaacggcaatgagc natttcccca gggaggccng cnttct 656 334 647 DNA Homo sapiensmisc_feature (1)...(647) n = A,T,C or G 334 acgcgggcgg gaagtgcagaggcaaatgca tttagtgttc ttcagcatgt cctcggtgct 60 gggccacatg tcaagaggggcagcaacacc gccagccatc tgcactaggc tgttgccaag 120 gcaactcagc agccatttgatgtttctgca tttaatgcca gttactcaga ttctggactc 180 tttgggattt atactatctcccaggccaca gctgctggag atgttatcaa ggctgcctat 240 aatcaagtaa aaacaatagctcaaggaaac ctttccaaca cagatgtcaa gctgccaaga 300 acaagctgaa agctggatacctaatgtcaa tggagtcttc tgagtgnttc ctggaagaaa 360 gtcgggtccc aagctctaagtgctggntct tacatgccac cattcacaag tctttaacag 420 aatgattcan tggctaatgctgatatcata aatgcgnaaa naaagtttgg ttctggcnag 480 aagtcaatgg cancaagtggnaaatttggg acatacncnt ttgtgataag tggaatactg 540 gngcncnctt acnggananacttaacgttn tttaanccaa acacaaccct tgaaagnnna 600 agctctaaan accattggcttttttcnggg ngnaaaaaag gcttaag 647 335 657 DNA Homo sapiens misc_feature(1)...(657) n = A,T,C or G 335 acaggtcaga gtcttctttt cttttctttttgagatggag tcttgctctg ttgccagact 60 ggagtgcagt ggtgcgatct gggctcactgcaatctccac ctcccgggtt caagcgattc 120 tcctgcctca gcctcccgag taactgggactacaggtgcg cgccaccaag cccagctcat 180 ttttgtattt ttagtagaga tggggtttcacgatgttggc taggatggtc tcgatctctg 240 gtcagagtct tttctgtaaa tatccttggtaaagaagcaa ttttagactg tagctgttgc 300 aaatgcttta aggaagaagc aaaacaactgtcaagtcttc ctgaaatgaa gaaactacac 360 cagggctgct atatcagagc aaccccaaccagcacttcaa tcatgatgcc nacagtggcc 420 cagctgagag accnggagaa agttccagatgcanagactg ngatgctctt gactatggaa 480 atattgcggc cagtaccaag ttagagaccaaacaggcata ngnncccgta ttaattggcg 540 tgaattttgc gataaganaa cttgggggggtgctgcggat nccatgatcn ccagaaaact 600 tnngggatgg ggtanaggcc catggcagaaaggttanggt ccttccaaag naaaana 657 336 649 DNA Homo sapiens misc_feature(1)...(649) n = A,T,C or G 336 ggtacgcggg caactatgga attccacagcgtgctctgcg gggtcactcc cactttgtta 60 gtgatgtggt tatctcctca gatggccagtttgccctctc aggctcctgg gatggaaccc 120 tgcgcctctg ggatctcaca acgggcaccaccacgaggcg atttgtgggc cataccaagg 180 atgtgctgag tgtggccttc tcctctgacaaccggcagat tgtctctgga tctcgagata 240 aaaccatcaa gctatggaat accctgggtgtgtgcaaata cactgtcagg atgaaaacca 300 cttaaantgg gtgncttgng ncccttntngcccaacagca acaaccctat tatcgtcttc 360 tgnggctggg acaaactggn taaaggatggaacctggcta actgnaagct gaaaaacaac 420 cacattgggc acacangcta tntgaacaccgngactggct ttttcagang gatcctntgn 480 gcttntggag gcaaggatgg gcaagccatgttatnggaac tcnaccaang caacaccttt 540 cacctttaan ggggggacat tatnaacgccttgggttaac cttaacgttn ttggtttgng 600 ctgcncaggc ccacattaaa aatgggatttaanggaaana catttnann 649 337 652 DNA Homo sapiens misc_feature(1)...(652) n = A,T,C or G 337 actcttggtt tgtcaatggg actttccagcaatccaccca agagctcttt atccccaaca 60 tcactgtgaa taatagtgga tcctatacgtgccaagccca taactcagac actggcctca 120 ataggaccac agtcacgacg atcacagtctatgcagagcc acccaaaccc ttcatcacca 180 gcaacaactc caaccccgtg gaggatgaggatgctgtagc cttaacctgt gaacctgaga 240 ttcagaacac aacctacctg tggtgggtaaataatcaaga gccttccggt cagtcccagg 300 ctgcagctgt caatgacaca ggaccctnactctactcagt gtcacaagga atgatgnaag 360 gaccctatga atgtggaatc cagaacgaattaaagcgttg accacagcga ccangcatcc 420 tgaatgcctt tttgggccan acgaccccaccattttcccc tcataccact attaccgtcc 480 aggggtgnac cttagncttt tcttgccatgcagcctttac ccaccttgac agnattcctg 540 gctggatgtt gggaacatna gnacncacncaagagctntt ttttccaaca tnatgggaaa 600 acanngnnct tatactgcag gccattacttngccntngcc cagnnggctn cg 652 338 651 DNA Homo sapiens misc_feature(1)...(651) n = A,T,C or G 338 ggtacatttg aacacacggc tgtgttaaagatgctgctaa tgtcagtcac tgggtgcact 60 aaaggatctc ttattttatg taaaacgttgggattgacaa gatagatctg acactctgtt 120 aagttaccct ctgaagctac ttcttgtgaaatactaatga cagcatcatc ctgccaagcg 180 aaagaggcag gcataagcaa ggacaaattaaaagggggta agagccttat catgatgagg 240 agtcttgntt tgacatcttg ggaaaagctgccatagtgtg aaagtcgtca atttctcacc 300 atggtttgca gtttgactgn ctctagttagggtgaagtct ctgagtggca cacaccttaa 360 gcctgaaggn tttcccttta aattttcattgagttggccc tcttcagcat atanggcttt 420 aagaacagaa canaccttgg ttttaagtgggtccatggga taaaatggga atggangact 480 ngaagaattc aagggctggg ccatctngcagtattctgaa tatcgaaaat ncnccaaggc 540 tgctatataa anccccctgg gcaanacttcaatcggaanc ccacggnggc ccnactnana 600 gncaggaccn ttccaantgg aacatnggantggggccttt gaggcnnggn n 651 339 634 DNA Homo sapiens misc_feature(1)...(634) n = A,T,C or G 339 actttttttt tttttttttt ttttttctagtttcagttat ttattgattt aatcattgta 60 atctccaata gagattacaa tagagatctccaacatgatt tcatgcattt agaggagaaa 120 tatttcctgg ttaagtggaa aattgtgcggatgtggcttc tggaanacct tcattctaaa 180 gcagcgttat agtgaaacat ttcatttanaaatctggacg ttccttcttc agcttgctgt 240 aatccacatt cactgagtag aacttgtattgatcattggg acccagtttg ttccagggct 300 ctgggttatt tctgcccaac aaacatctggattgaacaat gccagacgca agagatcagt 360 gttgctccag tagctccagt tccaataaatacnaagaggg ggatcaaagc tcggatgctt 420 cttggcctga ccgatgatct ggcggancatgtttgcngca aagtctccga ctggaaagga 480 ganaaccgcc taccccaagc cctaagctaaaaattatntg ccccgcgacc ttggncgnga 540 cccnctaagg caattccacc actggcggccgtctaangga tccacttggg ccaacttgng 600 naacatggca nactggtcct ggggaangtatccc 634 340 655 DNA Homo sapiens misc_feature (1)...(655) n = A,T,C orG 340 ggtactcttc cactcaggta tccgtgcggc cactccagca cacgcagtat gagcgcttca60 tcccctcggc ctacccctac tacgccagcg ccttctccat gatgctgggg ctcttcatct 120tcagcatcgt cttcttgcac atgaaggaga aggagaagtc cgactgaggg gctagagccc 180tctccgcaca gcgtggagac ggggcaggga ggggggttat taggattggt ggttttgttt 240tgctttgttt aaagccgtgg gaaaatggca caactttacc tctgtgggag atgcaacact 300gagagccaag gggtgggagt tgagataatt tttatataaa agaagttttt ccactttgaa 360ttgctaaaag tggnattttt cctatgtgca gtcactcctc tcatttctaa aatagggacg 420tggccaggca ccgtggctca tgcctgtaat ccacactttt ggaggncnng caagcggtta 480cgaagtcagg agatcgagac tattctggtt acacgnaaaa cctgncttac taaaagtacc 540tgcccggccg gccgntcaaa ggcgaatcca cacactggcg ggcgtactan tggatnccaa 600cttggaccaa cttggngnaa tatggcatac tggttcctgg nggaaatggt accnn 655 341 648DNA Homo sapiens misc_feature (1)...(648) n = A,T,C or G 341 acgaacctacagttttaact gtggatattg ttacgtagcc taaggctcct gttttgcaca 60 gccaaatttaaaactgttgg aatggatttt tctttaactg ccgtaattta actttctggg 120 ttgcctttgtttttggcgtg gctgacttac atcatgtgtt ggggaagggc ctgcccagtt 180 gcactcaggtgacatcctcc agatagtgta gctgaggagg cacctacact cacctgcact 240 aacagagtggccgtcctaac ctcgggcctg ctgcgcagac gtccatcacg ttagctgtcc 300 cacatcacaagactatgcca ttggggtaag ttgtgtttca acggaaagtg ctgtcttaaa 360 ctaaatgtgcaatagaaggn gatggtgcca tcctaccgnc ttttcctggt tcctanctgn 420 gtgaatacctgctacgtcaa atgcntacca ggttcattct ncctttnact aaaacacaca 480 ggtgcaacagacttgaatgc taagtatacc taattggata tgggatttaa ttttctttct 540 tacaancatttgtattgcta acaggccaaa atttcagtta cccttagggt ggttaacaat 600 cnaattaaacctgggaggca tacnttgnct aaatattact gnaaaaaa 648 342 342 DNA Homo sapiensmisc_feature (1)...(342) n = A,T,C or G 342 ggtacttttt tttttttttttttttttttt gttttttttt tttttttttt tttttttttt 60 tggctntana gggggtanagggggtgctat agggtaaata cgggccctat ttcaaanatt 120 tttaggggaa ttaattctaggacnatgggc atgaaactgn ggtttgctcc acanatttca 180 nagcattgac cgtagtatacccccggtcgt gtancggtga aagtggtttg gtttaaacgt 240 ccgggaattg catctgtttttaagcctaat gtggggacag ctnatgagtg caaaacgtct 300 tgngatgtaa ttattataccaatgggggct ttaatcggga at 342 343 484 DNA Homo sapiens misc_feature(1)...(484) n = A,T,C or G 343 ggtacgatgc ctagtgatga gtttgctaatacaatgccag tcaggccacc tacggtgaaa 60 agaaagatga atcctagggc tcagagcactgcagcagatc atttcatatt gcttccgtgg 120 agtgtggcga gtcagctaaa tactttgacgccggtgggga tagcgatgat tatggtagcg 180 gaggtgaaat atgctcgtgt gtctacgtctattcctactg taaatatatg gtgtgctcac 240 acgataaacc ctaggaagcc aattgatatcatagctcaga ccatacctat gtatccaaat 300 ggttcttttt ttccggagta gtaagttacaatatgggaga ttattccgaa cctggtagga 360 taagaatata aacttcaggg tgaccgaaaaatcagaatan gtgttggtat agaatggggt 420 cttcttcttc ngcggggtcn aanaaggtggtggtnccgcg tcctggccng gcnggcgctc 480 gaan 484 344 657 DNA Homo sapiensmisc_feature (1)...(657) n = A,T,C or G 344 cgaggtacgc gggattgttctggggcttgt cctcctttct gttacggtcc agggcaaggt 60 ctttgaaagg tgtgagttggccagaactct gaaaagattg ggaatggatg gctacagggg 120 aatcagccta gcaaactggatgtgtttggc caaatgggag agtggttaca acacacgagc 180 tacaaactac aatgctggagacagaagcac tgattatggg atatttcaga tcaatagccg 240 ctactggtgt aatgatggcaaaaccccagg agcagttaat gcctgtcatt tatcctgcag 300 tgctttgctg caagataacatcgctgatgc tgtagcttgt gcaaaaangg ttgtcccgtg 360 atccacaagg cattaagagcatgggtggca tggagaaatc gttgtcaaaa cagagatgtc 420 cgcagtatgt tcaanggtgtggagtgtaac tncagaattt tccntcttca ctcatttggc 480 tctctacatt aaggagtaggaaataantga aaggtcccct ccattaattt cccttcaaca 540 aataattttt tccgaaacnggaccaaatat ggccttcttn tagannataa tgtcntaagg 600 ggnatttatt ttaagcnncacanttttaat ttgcaaatna ctatctgggg aaaatac 657 345 662 DNA Homo sapiensmisc_feature (1)...(662) n = A,T,C or G 345 ggtacgcggg cgactcttagcggtggatca ctcggctcgt gcgtcgatga agaacgcagc 60 tagctgcgag aattaatgtgaattgcagga cacattgatc atcgacactt cgaacgcact 120 tgcngccccg ggttcctcccggggctacgc ctgtctgagc gtctcttgca aaaaaaaaat 180 aaannanaan acancaagtacaatttaatg cntanaaagg cctctctcca taaaactcan 240 cnctttacag atgtangaatatataagcnn tgccaaaatt actaatntgc cacatacnna 300 gcatcaattc caggtgctagtnagngggaa aaaaanttgg agaattcggc cctcgangag 360 ctccanannt taanctnccttactaantnc canggttctt tcaagcatgg aaaaattaat 420 ngtgctncat ngatnaangncttgtcattg ggccttnttt cctngacctg gcccggccgn 480 ccgttcnaaa ggctaaatccagacactgcg gccgttntaa tggttcnnac ttgggccaag 540 cttgggnaat catgggcaaagctgttcctg ggnnaaatnt tatccnctcc aattcncaca 600 natacgaanc tgaancttaagtgtnanntn gggngctaaa agtggcnaan ctcccttnat 660 gg 662 346 654 DNA Homosapiens misc_feature (1)...(654) n = A,T,C or G 346 acttcttggccgcctcacta gcactctccg cctgcttttt aaaggcttca ttggaggcca 60 gcagcgtggcctgctgcgaa atgagagtca ccaggcgtct aagcaggaag gacagcagcg 120 aggaaaagccagcaatgtag agattcctct gggcacggaa aagcttcatg tggaagtgct 180 ccatggccccgggattgttc tggaggttca ccttttccgt cacatcatca tacttccgaa 240 tttcgcgcacggcatcgatg accaacagca caaggatgac aatgagaacc acaaagaagg 300 tgttgccataggacactaac aactccacca gccgggactt gaaaatcttc tgccatcttt 360 taggagaaatgaagggaatg cagagaagca acacaacaaa gaccttcgca tagaggaagg 420 tggcaactgcagtccactgc agactcatcc tggtgctana agggttccac aggaagatgt 480 gaacttgttncgagtttcca cagtcaacgt gtcccccgta ccttnggccg ngaacacnct 540 taaggcgaattccaccactg cnggccgtct antggatcca actnggncca acttggcgaa 600 tatggcaaattgttctnggg naaatggttc ngtcaattcc ccantacnac cgga 654 347 536 DNA Homosapiens misc_feature (1)...(536) n = A,T,C or G 347 ggtactaatttaaggtaaca attctcgagg taaaataagg cattatagta acacaatttt 60 catgcctcagcaattaacaa tgattttcgt ttaattctct tccaactcta cagacataat 120 tctgctttcaccttcatcac gctttcatat ggttttaaca ggggatacac ctcctcttct 180 aagaatctctgcacctgctg ggaggcacga ccagtgaaag aagaaggatc cagtaaatga 240 tccaactgggagtgaatggg actgaagtag gcatcaacct ggatacgctc tatgaggnca 300 ttgcacccccttcctgctta accacagaag ctgcctgctg agaaagcact ctgattttct 360 catggcaatcctggcggcta ccttcacttt gaccatggcc atgatgatgg tctctgtggc 420 catgaaangcagctcttgcc gaatgcgccg tcaattactt tggggtacct gcccnggccg 480 gccgntcnaanggcgaattt cagccactgg cngncgtact agnggatcca actcgg 536 348 665 DNA Homosapiens misc_feature (1)...(665) n = A,T,C or G 348 ggtacgcggggagtcggcgt aggccttagg tgggttcgtg cgccttctac ctcgctgttt 60 cggttttcctggctcctcgg cccttttctc ccctgttgca gctgggagcg gacgaagcgc 120 gaagctgggattttttactg tctcctgaag aatttaacac aaacatggat atcagaccaa 180 atcatacaatttatatcaac aatatgaatg acaaaattaa aaaggaagaa ttgaagagat 240 ccctatatgccctgttttct cagtttggtc atgtggtgga cattgtggct ttaaagacca 300 tgaagatgagggggcaggcc tttgtcatat ttaaggaact gggctcatcc acaaatgcct 360 tgagacagctaccaggattt ccattttatg gtaaaccaat gccaatacag tatgcaaaaa 420 cagattcggatataatatca aaaatgcgtg gaacttttgc ttaaaaaaaa aaannnnnna 480 naaaaaagtcctgccnggcc gcccgttcaa anggcgaatt naccactggc ggccgttcta 540 gnggatccaactnggnacca acttggcgta atatggcaaa actggtnccg ngngaaatgg 600 tatccgttanaattcccaca cttcaaccgg aacctnaang taaacctggg gcctaagagn 660 gacnn 665 349474 DNA Homo sapiens misc_feature (1)...(474) n = A,T,C or G 349acttcgtcag tttgtaagac atgagtccga aacaactacc agtttggttc ttgaaagatc 60cctgaatcgt gtgcacttac ttgggcgagt gggtcaggac cctgtcttga gacaggtgga 120aggaaaaaat ccagtcacaa tattttctct agcaactaat gagatgtggc gatcagggga 180tagtgaagtt taccaactgg gtgatgtcag tcaaaagaca acatggcaca gaatatcagt 240attccggcca ggcctnagag acgtggcata tcaatatgtg aaaaaggggt ctcgaattta 300tttggaaggg aaaatagact atggtgaata catggataaa aataatgtga ggcgacaagc 360ncaaccatca tagcttgatn atattatatt tctgagtgcc agaccaaaga gaaggagtnt 420aaanggatga tcntcttttg ggcatcattt tgggaccttn ggccgggaac accc 474 350 452DNA Homo sapiens misc_feature (1)...(452) n = A,T,C or G 350 acgcggggaccgtggagagc agagcgcggc ggctggaagc tgctaagtca gagccgcgat 60 gttccggattgagggcctcg cgccgaagct ggacccggag gagatgaaac ggaagatgcg 120 cgaggatgtgatctcctcca tacggaactt tctcatctac gtggccctcc tgcgagtcac 180 tccatttatcttaaagaaat tggacagcat atgaagacag gacatcacat atgaatgcac 240 gatatgaagagcctggttac agtttcgact cctctctgca agtgaatagg cccagaaagg 300 tgtaagagactctttgaatg gacataaaat tctgcttgtt aagaacaagt ttggctctgg 360 taactgaccttcaaagctaa aatataaaac tatttgggaa agtatgaaac gatgtcttcg 420 tgatctggtgtaccttggnc gngaccacgc tt 452 351 616 DNA Homo sapiens misc_feature(1)...(616) n = A,T,C or G 351 ggtacgcggg aataattcca tagtcaagagcatcacagtc tctgcatctg gaacttctcc 60 tggtctctca gctggggcca ctgtcggcatcatgattgga gtgctggttg gggttgctct 120 gatatagcag ccctggtgta gtttcttcatttcaggaaga ctgacagttg ttttgcttct 180 tccttaaagc atttgcaaca gctacagtctaaaattgctt ctttaccaag gatatttaca 240 gaaaagactc tgaccagaga tcgagaccatcctagccaac atcgtgaaac cccatctcta 300 ctaaaaatac aaaaatgagc tgggcttggtggcgcgcacc tgtagtccca gttactnggg 360 aggctgaggc aggagaatng cttgaacccggnaggtggag attgcagtga gccagatcgn 420 acnactgnac tcagtctggc aantgagnaggcttccatct nanaangaan aganangang 480 actntnacct ggacctgccn ggccggtcgtttgngcaggt cnggagattt attcccttng 540 ggtggggngc nntaattggn tgntgggccnattcangttt tgggaatttc nncttggnnn 600 naaaanggga aatttt 616 352 603 DNAHomo sapiens misc_feature (1)...(603) n = A,T,C or G 352 ggtacggcacttggcgtaaa gccgcttccc tcaagagtaa ctacaatctt cccatgcaca 60 agatgattaatacagatctt agcagaatct tgaaaagccc agagatccaa agagcccttc 120 gagcaccacgcaagaagatc catcgcagag tcctaaagaa gaacccactg aaaaacttga 180 gaatcatgttgaagctaaac ccatatgcaa agaccatgcg ccggaacacc attcttcgcc 240 aggccaggaatcacaagctc cgggtggata aggcagctgc tgcagcagcg gcactacaag 300 ccaaatcagatgagaaggcg gcggttgcag gcaagaagcc tgtggtaggt aagaaaggaa 360 agaaggctgctgttggtgtt aagaagcaga agaagcctct ggtgggaaaa aaggcagcag 420 ctaccaagaaaaccagcccc tgaaaagaac ctgcagagaa gaaacctact acngaggaga 480 agaagcctgctgcataactc ttaaatttga atatttcntt aagggcnaat nttttggcag 540 gttctttggataagacntnt tttcngngtg ggaaaataan tnnnntattn nnggctntcc 600 tgg 603 353604 DNA Homo sapiens misc_feature (1)...(604) n = A,T,C or G 353ggtaccgact gtttttgaca actatgcagt cacagttatg attggtggag aaccatatac 60tcttggactt tttgatactg cagggcaaga ggattatgac agattacgac cgctgagtta 120tccacaaaca gatgtatttc tagtctgttt ttcagtggtc tctccatctt catttgaaaa 180cgtgaaagaa aagtgggtgc ctgagataac tcaccactgt ccaaagactc ctttcttgct 240tgttgggact caaattgatc tcagagatga cccctctact attgagaaac ttgccaagaa 300caaacagaag cctatcactc cagagactgc tgaaaagctg gcccgtgacc tgaaggctgt 360caagtatgtg gagtgttctg cacttacaca gaaaggccta aagaatgtat ttgacgaagc 420aatattggct gccctggacc tncagaccga agaagacccc aagtgtgtgc tgctatgaac 480atctttcaga gcctttcttg nacagctgga ttggcatctt cttaaagcca tgnttaaatt 540caacttanga ttaaaattaa aattcgtttt gcannatggc caatgcctgg actaacccan 600ggcn 604 354 631 DNA Homo sapiens misc_feature (1)...(631) n = A,T,C orG 354 ggtacttttt tttttttttt tttttttttt tttgggacgg agtcatgctc tgtcgcccag60 gctggagtgc agtggcatga tctcggctca ctgcaagctc cgcctcccgg gctcatgcca 120ttctcctgcc tcagcctccc gagtagctga gattataggc acctaccacc acgcccggct 180aatttttgta tttttagtag agacggggtt tcaccatgtt gaccaggctg gtctcgaact 240cctgacctta ggtgatccac tcgccttcat ctcccaaagt gctgggatta caggcgtgag 300ccaccgtgcc tggccacgcc caactaattt ttgnattttt agtaagagac agggtttcac 360catgttggcc aaggctgctc tttgaactcc tgacctcatg taatcgacct gcctttggcc 420ttccaaaagt gctgggatta ccaggtgtga gcccacaagc cccggnacct ggccnggcng 480gccgtttaaa agggcgaatt cagcacaatg gnnggccgta ctaaggggat ncnanctttg 540nanccaactt tgggggaaat atggggcana actggttcct ngngnaaatg gtaaccgtta 600caaattcccn caaanttttg nnccgggagg n 631 355 626 DNA Homo sapiensmisc_feature (1)...(626) n = A,T,C or G 355 ggtacgatgc ctagtgatgagtttgctaat acaatgccag tcaggccacc tacggtgaaa 60 agaaagatga atcctagggctcagagcact gcagcagatc atttcatatt gcttccgtgg 120 agtgtggcga gtcagctaaatactttgacg ccggtgggga tagcgatgat tatggtagcg 180 gaggtgaaat atgctcgtgtgtctacgtct attcctactg taaatatatg gtgtgctcac 240 acgataaacc ctaggaagccaattgatatc atagctcaga ccatacctat gtatccaaat 300 ggttcttttt ttccggagtagtaagttaca atatgggaga ttattccgaa gcctggtagg 360 ataagaatat aaacttcagggtgaccngaa aaatcagaat aggtgtttgg tttagaatgg 420 ngtcttctnc ttcngctggggtnnaagaan gtnggggttc nngcgtnctn gntcgggcgg 480 ntggttttaa nggccnaaattcnngnataa ttggcggcng ttactaagng gnatctanct 540 tggtnccaaa nttggngntaatcatggtnc tagctngtnc tcngtgntaa attggntncc 600 tgttaaattn tntnnaatnttntggc 626 356 617 DNA Homo sapiens misc_feature (1)...(617) n = A,T,Cor G 356 actttttttt tttttttttt tttttttcta gtttcagtta tttattgatttaatcattgt 60 aatctccaat agagattaca atagagatct ccaacatgat ttcatgcatttagaggagaa 120 atatttcctg gttaagtgga aaattgtgcg gatgtggctt ctggaanaccttcattctaa 180 agcagcgtta tagtgaaaca tttcatttan aaatctggac gttccttcttcagcttgctg 240 taatccacat tcactgagta naacttgtat tgatcattgg gacccagtttgttccagggc 300 tctgggttat ttctgtccca acaaacatct ggattgaaca atgccagacgcaagagatac 360 agtgttgctc cagtagctcc agttccaata aatacnaaga gggggatcaagctcggatgc 420 ttcttggcct gaccgatgat ctggccggaa ncatgtttgc cggcaaaaggctccnacttg 480 ggaaagggga naacccgcct aaccnccagg gcctaagctt aaaatttttggccccgggta 540 ccttggccgg gaccccctaa gggngnaatt ccnncccctt ggggggccgtttaangggan 600 ccaacttggn ccaaatt 617 357 611 DNA Homo sapiensmisc_feature (1)...(611) n = A,T,C or G 357 ggtacttttt tttttttttttttttttttt ttttaggcaa agaactttat taatctttgt 60 ttcaaacttg attcccaggcttcttcggct taattagctg caaagaatga attgngtata 120 agcaaaaact gaaaagagctgcagtgtcca aggggcttgg gcttaaaaat attagagatc 180 tagattttat cagatccataaacaaaaatt tcttaaaaag cagtcataat ataaaatagc 240 agctcccagt aacttcttcaggntttatct tcagaagttg actcaattca gtttgcctca 300 ttcttggaag cctcatcaaaattctccaca agatctggaa cttcatcatc atcatcctct 360 ccagtaacaa gtggngcttttccatcccca gantggttgg gcanaacttt ngnccagctc 420 cttaacttag cagactattcggacccaagc tnggttnaaa aanctgggaa cnatttntgn 480 naactggttt ggttnaacanggcntgnaag ggggaaaggn gtnccctgcc caaaaaaccn 540 ggacctttag ggtgnnaaaggggacctggc cctgggttgg aaccaantcn ccttttnana 600 ccnnanaatn g 611 358 619DNA Homo sapiens misc_feature (1)...(619) n = A,T,C or G 358 ggtacttttttttttttttt tttttttttt ttgagatgga gtctcgctct gtcgcccagg 60 ctggagtgcagtggcgcaat ctctgctcac tgcaacctcc gcctcctggg ttcaagcaat 120 tctcctgtctcagcctccca aatagctggg attacgggca tgtgtcacga cgctcggcta 180 atttttgtatttttagtcga gacgaggttc caccatgttg gctaggctgg tctcaaactc 240 ctgacctcaggtgatccgcc tgcctcggcc tcccaaagtg ttaggattac gggtgtgagc 300 cactgcgcccagcaagcaac ctagatttta aaacaacatg agataaataa gcctaattgg 360 atttaactacatctaacatt tttactaata gttgnaatac tggtagaatt tggaaactat 420 tatatatattatgcngaaaa gtaaataatt ctggtaaaat canttanggn ccntgaattt 480 nagcataggggaaaaaaaga tgccntttta aatccaataa gtaaaaaccn tttaaccctn 540 tntttaaattggaanttccc cccaatttnt tattaatttc aacttntttt gaaaactcat 600 ntttccnaaaantnggggg 619 359 624 DNA Homo sapiens misc_feature (1)...(624) n =A,T,C or G 359 ggactttttt tttttttttt tttttttttt ttttttggag gaaaacccggtaatgatgtc 60 ggggttgagg gataggagga gaatggggga taggtgtatg aacatgagggtgctttctcg 120 tgtgaatgag ggttttatgt tgttaatgtg gtgggtgagt gagccccattgtgttgtggt 180 aaatatgtag agggagtata gggctgtgac tagtatgttg agtcctgtaagtagganagt 240 gatatttgat caggagaacg tggttactag cacagagagt tctcccagtaggttaatagt 300 ggggggtaag gcgaggttag cgaggcttgt tanaagtcat caaaaagctattagtgggag 360 tagagtttga agtccttgag agaggattat gatgccactg ngaatgcnttcctaatttga 420 gtttgctagg cagaatagtn atgaggatgt aaacccctng gccaattattaaaaatgact 480 gcncccgtga aacttnaggg ggtttggatt aaaaangctt gtacttccaanggctntntg 540 gcctnattta aaaaatttcc ctnnncnaat ttagggcttn ttnncnnaagccnanagggn 600 ccccnancct ttcccggggg ggcn 624 360 611 DNA Homo sapiensmisc_feature (1)...(611) n = A,T,C or G 360 acgcggggag gcggaggcttgggtgcgttc aagattcaac ttcacccgta acccaccgcc 60 atggccgagg aaggcattgctgctggaggt gtaatggacg ttaatactgc tttacaagag 120 gttctgaaga ctgtcctcatccacgatggc ctagcacgtg gaattcgcga agctgccaaa 180 gccttagaca agcgccaagcccatctttgt gtgcttgcat ccaactgtga tgagcctatg 240 tatgtcaagt tggtggaggccctttgtgct gaacaccaaa tcaacctaat taaggttgat 300 gacaacaaga aactaggagaatgggtaggc ctttgtaaaa ttgacagaga ggggaaaccc 360 cgtaaagtgg ttggttgcagttgtgtagta attaangact atggcaagga gtctcagcca 420 aggatgtcat tgaagagtatttcaaatgcc agaaatgaag aaattaaatc nttggcttac 480 ttaaaaaaaa annnnnnnnnaaaaaaaagg tccttgggcg gnacaccctt aaggggnaat 540 tcnnnnccct gggggccnttataangggnn ccnacttggg ccaaattggg naaananggg 600 naaanttttt n 611 361 404DNA Homo sapiens misc_feature (1)...(404) n = A,T,C or G 361 acatattttaatagaaagat acaacctttt tattttcact ccttttattt ctgctgcttg 60 gcacatttttgagttttccc acattatttg tctccatgat accactcaag cagtgtgctg 120 gacctaaaatactgacttta gttagtatcc ttggattttt agattcccag tgtctaattc 180 cctgttataatttgcgcaaa caaaacaaaa tgttatgata atctttctcc actgttctaa 240 tatatattgtatttttattt gatagcttgg gatttaaaac atctctgttg aaggcttttg 300 atccttttgagaaataaaga tctgaaagaa atggcataat cttaaaactt gataaaaaaa 360 aaanannnnnnnnaaaaaaa aaagtacctn ggccgngacc acgc 404 362 322 DNA Homo sapiensmisc_feature (1)...(322) n = A,T,C or G 362 ggtacttttt tttttttttttttttttttt ttttttggag ttgtaggcaa atgtttaatt 60 aattctgctc atatgcacatctgaaagcat gagacacact ccacagacag cacgcactgg 120 ggctggtggg gcanatgggcactcgccgat taggtattaa tgtcaataat acgtgcataa 180 agtgctgata aaataacttaagtgttacaa aaagagacag tccacggtgg ctgcaggcac 240 atgcaggcgg gactgggtcagacactccag ggctgcacat gttccagctg gcctgagtcc 300 gacacgtcat agctggcctt gt322 363 616 DNA Homo sapiens misc_feature (1)...(616) n = A,T,C or G 363cgaggtacgc gggctaagca agggaaaaat aacagtttct ctgagccaga gaagacttga 60tcacagttct ccaagcatcg tgatagcaat gcttaacccc aggaagattt caaggcaggg 120agaagaacat ttcaaataag attcttgtta acccatttat gcctagtgtt ccattattgg 180aatgctaagc ttgtgggagt catttacatc ctactgctca aagtcattgc caaggtctga 240tttttcacac aaaaaattgc aacccccagc ataaatgttt agctactgtc atcagttagc 300aaattcatcc acacaaacac aattagagtt tggttttttt ttaagctttt caaaacttac 360taaactggca caattttata tgtatgctat ttggtgnatt tatgcttaag agcnaaaaag 420tttgatggga ttttaaattc angccaagcc tacacgctga gacaatccct acaaccatgg 480nagtaactaa ngaaccttta tctaagnttt taagttttaa anggagngct taatggttca 540ngtctangtt ggaatttcct tcanaaattt cntcttttaa aaaattttcc caaaatnggt 600ccttaaaaaa ctcann 616 364 618 DNA Homo sapiens misc_feature (1)...(618)n = A,T,C or G 364 cgaggtacgc ggggcttctc gcctaacgcc gccaacatggtgttcaggcg cttcgtggag 60 gttggccggg tggcctatgt ctcctttgga cctcatgccggaaaattggt cgcgattgta 120 gatgttattg atcagaacag ggctttggtc gatggaccttacactcaagt gaggagacag 180 gccatgcctt tcaaatgcat gcagctcact gatttcatcctcaagtttcc gcacagtgcc 240 caccagaagt atgtccgaca agcctggcag aaggcagacatcaatacaaa atgggcagcc 300 acacgatggg ccaagaagat tgaagccaga gaaaggaaagccaagatgac agattttgat 360 cgttttaaag ttatgaaggc aaagaaaatg aggaacagaataatcaagaa tgaaagttaa 420 agaaacttca aaaggcagct nttctgaaag cttnttcccaaaaaagcacc tgggtacctg 480 gccgggccgg ccgtttaaaa gggcnaattc caccactggcggccgtctan ngggatccaa 540 cttnggacca acttggngga atatggcnaa attgttcctggggnaaatgt ttncgttcaa 600 attncncaaa ttacggcc 618 365 601 DNA Homosapiens misc_feature (1)...(601) n = A,T,C or G 365 acgtcctggaggactctatt gtggacccac agaatcagac catgactacc ttcacctgga 60 acatcaaccacgcccggctg atggtggtgg aggaacgatg tgtttactgt gtgaactctg 120 acaacagtggctggactgaa atccgccggg aagcctgggt ctcctctagc ttatttggtg 180 tctccagagctgtccaggaa tttggtcttg cccggttcaa aagcaacgtg accaagacta 240 tgaagggttttgaatatatc ttggctaagc tgcaaggcga ggccccttcc aaaacacttg 300 ttgagacagccaaggaagcc aaggagaagg caaaggagac ggcactggca gctacagaga 360 agccaaggacctcgccagca aggcggccac caagaacagc agcagcagca acagtttgtg 420 taaccagnctaccaacaaca nagnacccca nacaggtagg cttacccctt tggcctcctt 480 taatggaccttggccgggaa cacccttang gcgaattcag ncactggggg ccgtactang 540 ggatccncttggaccaactt ggggaaacag ggcaaaattg ttcttgggga aattntatcc 600 n 601 366 321DNA Homo sapiens 366 actttttttt tttttttttt tttttttgag atggagtctcactctgtcgc ccaggctgga 60 atgcagtggt gcaatctcag ctcactgcaa cttccacctcccaggttcaa gtgattctcc 120 tgcctcagcc tcccacatat ctgggactac aggtgcacaccaccatgccc agctaatttc 180 tttgtatttt ttagtagaga cggggtttca tcttattgggcaggctggtc tcgaactcct 240 aaccttgtga tctgcccacc tcggccttcc aaagtgctgggattacaggc gtgagccacc 300 gtgctcggcc acccgcgtac c 321 367 264 DNA Homosapiens 367 actgatcatg gagttaatca acaatgtcgc caaagcccat ggtggttactctgtgtttgc 60 tggtgttggt gagaggaccc gtgaaggcaa tgatttatac catgaaatgattgaatctgg 120 tgttatcaac ttaaaagatg ccacctctaa ggtagcgctg gtatatggtcaaatgaatga 180 accacctggt gctcgtgccc gggtagctct gactgggctg actgtggctgaatacttcag 240 agaccaagaa ggtcaagatg tacc 264 368 488 DNA Homo sapiensmisc_feature (1)...(488) n = A,T,C or G 368 ggtacagatg cacaggaggccatagggttt aggcanaggg gagcacaaan gttgaagatg 60 aggcgctgcc atcaatgctgggacttcagg cnaagggcag gaactgagga agccacaagg 120 gaggacattt tctgcagttgctgaancagt ancaactagg tcctgagaaa gccctntctc 180 gtggaagaat aacagccaggcnggaaagct tttcatcctg caaagctggg gaagaagatt 240 cttccttaaa ttgtcatctgcacttcagct cangaatcct gttggctgaa gtccagagtg 300 tccntttctg attcctgaagtanatnaaca gcccngnccc aangaagagn aggnntagta 360 caaagccnnc tncgcgtacctgtncgggcg gnngttcgna aggntcaaat tccagcacaa 420 ttgnctgccg ttantagttggattctnact ttngtactta ncttggcgta ntttatggtn 480 ataanttg 488 369 602 DNAHomo sapiens misc_feature (1)...(602) n = A,T,C or G 369 acgggggtttcactacttct cccccggact ccttggtagt ctgttagtgg gagatccttg 60 ttgccgtcccttcgcctcct tcaccgccgc agaccccttc aagttctagt catgcgtgag 120 tgcatctccatccacgttgg ccaggctggt gtccagattg gcaatgcctg ctgggagctc 180 tactgcctggaacacggcat ccagcccgat ggccagatgc caagtgacaa gaccattggg 240 ggaggagatgattccttcaa caccttcttc agtgaaacgg gtgctggcaa gcatgtgccc 300 cgggcagtgtttgtagactt ggaacccaca gtcattgatg aagttcgcac tggcacttac 360 cggcagctcttcaccctgag caactcatca caggcnagga aaaatgctgc aataactatc 420 ccgaaggcactacaccattg gcaaggagaa taattgacct gtgttggacc gaattcgcaa 480 gctggctgaccatgcaccgg cttaagggtt nttggttttc ccaacttttg gggggggaac 540 tgggtttngggtaaccctnn tggtnatngg aacgntttta antggatttt gggaanaaan 600 cc 602 370257 DNA Homo sapiens misc_feature (1)...(257) n = A,T,C or G 370actttttttt tttttttttt tttagttttt ttttattttt tacaaatata ctggagaatc 60atgcaatgct gccagcattg gatgcaatcc ggggccacaa gtctgcacac tcctttgcta 120ctggtcctgt aatggcagaa cctttcatct cgcctttatt gntcactatg actcctgcat 180tatcttcaaa ataaagaaac acgccatctt ttctacggta tgactttcgt tgtcgaatga 240ccactgctgg atgtacc 257 371 607 DNA Homo sapiens misc_feature (1)...(607)n = A,T,C or G 371 actttttttt tttttttttt tttttttgct atttagtttttatttcataa tcataaactt 60 aactctgcaa tccagctagg catgggaggg aacaaggaaaacatggaacc caaagggaac 120 tgcagcgaga gcacaaagat tctaggatac tgcgagcaaatggggtggag gggtgctctc 180 ctgagctaca gaaggaatga tctggtggtt aagataaaacacaagtcaaa cttattcgag 240 ttgtccacag tcagcaatgg tgatcttctt gctggtcttgccattcctgg acccaaagcg 300 ctccatggcc tccacaatat tcatgccttc tttcactttgccaaacacca catgcttgcc 360 atccaaccac tcagtcttgg caagtgcaga tgaaaaactgggaaccantt ggggttgggt 420 ccacatttgc catggacaag aatgccagga acccgtatgctttaaggatg aagtctcatc 480 ttcaaaattc ttccccataa atggacttgc caccagngccattatggcgt gtgaagtccc 540 cancctggcc cataaaccct ggaaaaatnt tggnaaaccggaaccctttt aaccaatcct 600 ttttttc 607 372 607 DNA Homo sapiensmisc_feature (1)...(607) n = A,T,C or G 372 acgaatgtgg gaattactcaggagcagcag aatatcttta tttttttaga gtgctggttc 60 cagcaacaga tagaaatgctttaagttcac tctggggaaa gctggcctct gaaatcttaa 120 tgcagaattg ggatgcagccatggaagacc ttacacggtt aaaagagacc atagataata 180 attctgtgag ttctccacttcagtctcttc agcagagaac atggctcatt cactggtctc 240 tgtttgtttt cttcaatcaccccaaaggtc gcgataatat tattgacctc ttcctttatc 300 agccacaata tcttaatgcaattcagacaa tgtgtccaca cattcttcgc tatttgacta 360 cagcagtcat aacaaacaaggatgttcgaa aacgtcggca ggttctaaaa agatctaggt 420 taaaggttat tcaacanggagtcttacnca tntaagaccc cattacngga atttggtgaa 480 tggttatatg taactttgactttaangggc tcaaaaaaag ctnaggggat gtgaatcaag 540 cttgngaagg ctttttttgggggctngntt nngggtttnt tgnaaagncc ngttttnntt 600 ttggaat 607 373 618 DNAHomo sapiens misc_feature (1)...(618) n = A,T,C or G 373 acttttaatgtttgctgttc aaacgaaaat agattggatc ttggttaagt tcacttggtt 60 tggccaggcacagtggctca cgcctgcagt cccagcactt ggggaggtgg aggcgggccg 120 atcacctgaggtcaagagtt tgagaccagc ctggctaacg cggtgaaacc ccatttctac 180 taaaaatacaaaaaattagc tgggcgtggt ggtgcgcgct tgtaatccca gctactcggg 240 aggctgaggcaggagaatcg cttgagccag agaggcaaag gttgcaataa gccaagatag 300 cgccattgtattccagcttg gacaacaaga gcgaaactct gtctaaaaaa aaaaaaaaaa 360 cacacacacaacacaatatt ttcacgcctg taaacctagc acattgggaa gccaaggtgg 420 gaggattgcttgaggccagg agttcaaggc ttgcantgag ctatgaatgn acactgnacc 480 tttggncgngaacacnctta nggccaaatt ccngcacact tgggggccgg tactaanggg 540 atcccanctttggnnccaaa nttggngnaa acatgggcaa aattggtncc tggngaaaat 600 ggttccgttccaaatccc 618 374 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,Cor G 374 acccagctgc tgcccacatt tctggtccag agtcccgaac cccgagcactgggatgcctg 60 gctactccga gcgttatcca gactagcgag tgggaggcag atgtaaaatctggaacgcag 120 attttagttt gttggaagga gaaatgtaac atagtgaacc acgcatctctggagggtgta 180 aagcagagac agccaagagc caaggcactg atgtttgaac tggaaacttcaaaacgttta 240 ataagagtct tcaggatggg tttgaactag acaagctaga aatttctttagaacaccagc 300 tctagcatgc atctcccact tttggctttc ctggagagga gcttgaagaggtggttctgc 360 agacagccac agtgatactc aggaaacnca gaggaatgga tttgacttttctgctaggaa 420 tctttggtat aagttctcct tgagttgtaa gangcatgga aatatacatgaaactgaana 480 acctgcaagg aanggaaatg ggaacntttc atctgagtgn aaactaaccaagtnggcaat 540 ttngacttga aacccttgaa accttcnagt ccaantcctg gtttgggggataaangaacc 600 ggncn 605 375 602 DNA Homo sapiens misc_feature(1)...(602) n = A,T,C or G 375 acggatgcta cttgtccaat gatggtaaaagggtagctta ctggttgtcc tccgattcag 60 gttagaatga ggaggtctgc ggctaggagtcaataaagtg attggcttag tgggcgaaat 120 attatgcttt gttgtttgga tatatggaggatggggatta ttgctaggat gaggatggat 180 agtaataggg caaggacgcc tcctagcttgttagggacgg atcggagaat tgtgtaggcg 240 aataggaaat atcattcggg cttgatgtggggaggggtgt ttaaggggtt ggctagggta 300 taattgtctg ggtcgcctag gaggtctggtgagaatagtg ttaatgtcat taaggagaga 360 atgaanagaa gtaagccgag ggcgtctttgattgtgtagt aagggtggaa ggtgatttta 420 tcggaatggg aagtgattnc taaggggntgtttgancccc gtttgtgcca gaatangaag 480 tggaatgctt cttanggctt caataaatgaanggcanaat gaattgaaag gtaaanaaac 540 cntnaagggt ggacttgtta ctgataaccntcctaaaatc attgccccgn aacttggccg 600 gg 602 376 611 DNA Homo sapiensmisc_feature (1)...(611) n = A,T,C or G 376 acgcgggatc gaagaattcacaaaaaacaa tagcctcatc atccccacca tcatagccac 60 catcaccctc cttaacctctacttctacct acgcctaatc tactccacct caatcacact 120 actccccata tctaacaacgtaaaaataaa atgacagttt gaacatacaa aacccacccc 180 attcctcccc acactcatcgcccttaccac gctactccta cctatctccc cttttatact 240 aataatctta taaaaaaaaaaaaaaaaaaa aaaaaaaaaa ncaaaaaaaa aaaaanaaaa 300 aaaaaaaang tncngccatttttngtttcn ggtaaacngg aatataangn gaaagaacaa 360 acnttggaac atacttaatggatttttata gaactttgna aaccaaagga gattcatgtt 420 ttanaagtct ggccttttttatatcttgga agaaaattat gtntggaggc tntaaataaa 480 tcccattatt ttctcagggaatctgggtag gaattgccgg catgggaant tttnnggggc 540 cggatnggaa agtttggcctaanaaatngc nctttntnaa naattttgga attttgggaa 600 gcccnaagca n 611 377 367DNA Homo sapiens misc_feature (1)...(367) n = A,T,C or G 377 acgcgggccgtttggcatct ctgccctcat cgtgggtttc gactttgatg tcactcctag 60 gctctatcagactgacccct cgggcacata ccatgcctgg aaggccaatg ccataggccg 120 gggtgccaagtcagtgcgtg agttcctgga gaagaactat actgacgaag ccattgaaac 180 agatgatctgaccattaagc tggtgatcaa ggcactcctg gaagtggttc agtcaggtgg 240 caaaaacattgaacttgctg tcatgaggcg agatcaatcc ctcaagattt taaatcctga 300 agaaattgagaagtatgttg caaaaaaaaa aananaaatn aaanaagtac ctcggccgng 360 accacgc 367378 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,C or G 378ggtacctgga tcctgctcct ctgggttgaa acccgggcgc cgccaagatg ccggcttacc 60actcttctct catggatcct gataccaaac tcatcggaaa catggcactg ttgcctatca 120gaagtcaatt caaaggacct gcccccagag agacaaaaga tacagatatt gtggatgaag 180ccatctatta cttcaaggcc aatgtcttct tcaaaaacta tgaaattaag aatgaagctg 240ataggacctt gatatatata actctctaca tttctgaatg tctgaagaaa ctgcaaaagt 300gcaattccaa aagccaaggt gagaaagaaa tgtatacgct gggaatcact aattttccat 360tcctggagag cctggttttc cacttaacgc aatttatgcc aaacctgcaa acaaacaggg 420aagatgaagt gatgagagcc tatttacaac agcttaaggg caagaaactg gactggaact 480ttgtgaagaa gttttcgacc cttagaatgg ttaaaccnac agtgggggga cttgcttttg 540gaaaanaccg tttattgacn anagtttttt tggactggan atgaaaggng cccnggttng 600ccccggtttn n 611 379 602 DNA Homo sapiens misc_feature (1)...(602) n =A,T,C or G 379 acagctggtt ggacctattc atgcatcttc accagcagct ggagcatctccacccttggt 60 atttctggtg taaattactt gagctctgtg ctttgaaacc agtttgataagtcctttact 120 aaggagctcc tgaagggctg ccctggccag ggagcctcga atcttcagtctctcagagac 180 cacagctggg gttataagtt tatagttggg aacttcctta cagagtttatcataggtagc 240 tttgtcaaac aagactaagt tattgagctt gtcccgaact ttgcctttggaccacttctt 300 ctttttggcc ttgcccccgg atttgttcac tgggtctttg nctttcttggccgactttcc 360 agcgtccttc ttcttcttgt cgtccttagg cggcattgcc aagctcggagaatagcanca 420 gacacngnaa cctngtcaag atgtcngaca aaaagccccg ggtaccttgggcgngaacac 480 gcttaaggcg aattccacac actggcggcc gtactanggg gatccagcttnggaccaact 540 tggnggaaac atggcnaact gnttcctngn ggaaaatgtn atccgttaaaattnccccaa 600 at 602 380 598 DNA Homo sapiens misc_feature (1)...(598)n = A,T,C or G 380 ggtacngcgg ggggtgcctg gctccgtttc ctgcttttggttcttacagt agtcggcgta 60 ggccttagat tttttactgt ctcctgaaga atttaacacaaacatggata tcagaccaaa 120 tcatacaatt tatatcaaca atatgaatga caaaattaaaaaggaagaat tgaagagatc 180 cctatatgcc ctgttttctc agtttggtca tgtggtggacattgtggctt taaagaccat 240 gaagatgagg gggcaggcct ttgtcatatt taaggaactgggctcatcca caaatgcctt 300 gagacagcta caaggatttc cattttatgg taaaccaatgcgaatcagta tgcaaaacag 360 attccggata taatatcaaa aatgcgtgga acttttgttgccaagaaaag aanaaagaaa 420 agaaaaagnc caaacttggg aacaactgna caaccncaaccaaaaanctg ggcnngggac 480 tccaaatcac ttatacccag ggaattcacc ccnaatcttaggtcctgata ccttcaacta 540 tatttaatcc ttaaaactta nccgaagagc taatngatgatgtntcctgc cggtaacn 598 381 631 DNA Homo sapiens misc_feature(1)...(631) n = A,T,C or G 381 ggtacgcggg gagagtgtgg tcaggcggctcggactgagc aggactttcc ttatcccagt 60 tgattgtgca gaatacactg cctgtcgcttgtcttctatt caccatggct tcttctgata 120 tccaggtgaa agaactggag aagcgtgcctcaggccaggc ttttgagctg attctcagcc 180 ctcggtcaaa agaatctgtt ccagaattccccctttcccc tccaaagaag aaggatcttt 240 ccctggagga aattcagaag aaattagaagctgcagaaga aagacgcaag tcccatgaag 300 ctgaggtctt gaagcagctg gctgagaaacgagagcacga gaaagaagtg ctttagaagg 360 caatagaaga agaaccacaa cttcgtaaaaatggcngaan aagaaactga ccnccaaaat 420 gggagcttat taaagagaan ccagangnnccaatngnttg gccaactggg accgtttgca 480 anaagaaggg ttagccccnt tgaanaaatgccggaagaac caaagaattc caagaccctt 540 gntgcnaaac ttgaacttgc ctaattggtcttgagaactg cttttttccc atcccttcta 600 aaatccaaaa atgnacctgc ccgggggccg t631 382 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 382acattcccag atttttaagc ctccctcata aacacctgta atcagatcag agtgagaaga 60aaagcttttt gaaactatgt tttctccagg gaagttctct ttcaacaaga tggttttcac 120tactgataac ttaacatgct ggaaacctgg taatgtttct atgactttat tttctaacat 180cttctttaaa tctttaggca tagcatgctc tttggcagct ctcaaggagg gctgtttcca 240tgtggctcca agttccttga actgctggct gcactgagtg gactgtctgt gtcttgagag 300ggagctgcat tttcattgac ttatggtccc acaagtgacc ctgaggcaan gtcnaattgg 360tctncanaac atttttggcc ctctcttctc ctttttgact tttctgagac tgacagttct 420tttganggaa tccagggnna angcttccnt ctctaatggg ggntaaattc attttccaaa 480anggncggtt tttgggaaaa tnaaanttga aanggcatcc nttttattaa tgccccnanc 540ttttaanttc ngattntnaa cttnctgnta gaatttgtgg atccnccaaa ttggcttaat 600attcaaatag ctt 613 383 628 DNA Homo sapiens misc_feature (1)...(628) n =A,T,C or G 383 ggtactttga ccctggaaag gtatgggtct gcttaaaaga aagaagaaacatacacgtaa 60 tcaaataaag cttaacatta tgcagggctt ataatcattt tcagcaacggactgcaagct 120 gcactgtgaa gaaaatgcat agcagaggag aaagctgggg atctgaggaaataggtaagg 180 aaaacagtgt caacacacag tggaagaagt gatgaagaca tctattccggagctcacgtg 240 ccatgccctg ctagcgttcc ttaacaagcc acctgctcca gaaggccacagcctgaccct 300 cccaagtgga atataaatgc ccaagtgcca catgaagcca ccttctncactacctaaaaa 360 ggttgtctgg gactgagctc agaacacaca cctttctggg ctaccaaacctttaagtgga 420 aagaattttt tnctaaatat ctanttttna tacccacttt aacgccacttttatattgaa 480 attgggcttc taattagncc ctttcctcaa ttccttagga nggaactcataatgggagcc 540 aaccaaccag ggattctacc cccaatngac tgnnctttaa angtattattaattttgang 600 ggcaaaggtg tgaatggttt acaatacc 628 384 620 DNA Homosapiens misc_feature (1)...(620) n = A,T,C or G 384 acaggtaagccctggctgcc tccacccact cccagggaga ccaaaagcct tcatacatct 60 caagttgggggacaaaaaaa gggggaaggg ggggcacgaa ggctcatcat tcaaaataaa 120 acaaaataaaaaagtattaa agcgaagatt aaaaaaattt tgcattacat aatttacacg 180 aaagcaatgctatcacctnc cctgtgtgga cttgggagag gactgggcca ttctccttag 240 agagaagtggggnggctttt angatggcaa gggacttcct gtaacaatgc atctcatatt 300 ttggaatgactattaaaaaa acaacaatgt gcaatcnaaa gtctcggccc atttgcggaa 360 ctttggggggatgcttgctt cnaccgantt ggtgncaacc tttnnccggt tccanttttt 420 naaattcttagtnnaagcnn aaaaanntag aatancncna nancataact tannaancca 480 tttaanaggtccctcggccg gaacnnnctt aanggtnaat cccantnnnt ggcgggcgtt 540 actncnggatccanccttgg nnccaaantn gnggaattca tggcnnaacc gntcctgggn 600 gaantngttnccttnaaanc 620 385 535 DNA Homo sapiens misc_feature (1)...(535) n =A,T,C or G 385 ggtacttttt tttttttttt tttttttggt atttagtttt tatttcataatcataaactt 60 aactctgcaa tccagctagg catgggaggg aacaaggaaa acatggaacccaaagggaac 120 tgcagcgaga gcacaaagat tctaggatac tgcgagcaaa tggggtggaggggtgctctc 180 ctgagctaca gaaggaatga tctggtggtt aagataaaac acaagtcaaacttattcgag 240 ttgtccacag tcagcaatgg tgatcttctt gctggtcttg ccattcctggacccaaagcg 300 ctccatggcc tcacaatatt catgccttct ttcactttgc caaacaccacatgcttgcca 360 tccaaccact cagtcttggc agtgcagatg aaaaactggg aancntttggggtngggncn 420 acatttgcct tggccaaaat gccnggaacc ggccccgtac cttgnccnggccggccggtt 480 caaaagggcg aattccacac acttggcggg ccgtactang gggatccaacttcgg 535 386 642 DNA Homo sapiens misc_feature (1)...(642) n = A,T,C orG 386 acagcattgg cagtggtgcg tcagaggtgg cagaactatt tcacactaac cagttgaaga60 ctacacaaga ttaataccat ccagcatcag gatatagctg tggattttac aaaccattct 120tatttctaac ttcaggagtt gatgtttttc ccagtccatc ttaaaatatt actgctttaa 180tcacagatca ggtaaaaagg acaacatgca caacctccac ctagaatcct gttgtagcct 240agacagtgaa atgatatgac atcagaagac tttaaaattg cagctccttt tggatccccc 300aaagtgtatc tgcactcttc ttcaaacggg ccctctttcc tcaagaagtc agaagtcacc 360ttcacaangn ctgagaattc cattctgnnc ccaaantgca agggacactn aaggaagaca 420tcattctttt attccgtnaa agacccttaa ttcatgggng gaaactgggt gcacccgcct 480nagaatcttt attanactct ttgnccaatt tggttacaga agagntncan tanccccang 540aannggtagc ctttggagtt tgantcaccc tcataagcac ccttaaacca cctgnttggg 600gaaccttctt tcactggtcc ctaactttat tangccctaa ag 642 387 256 DNA Homosapiens 387 ggaccttttt tttttttttt tttttttttt tgaaaagaaa ggccttacatatttattact 60 gaatccagcc aaccaacgtg ttcataacag attcagagag gaaaacacgtcgaaatctcc 120 agatagtggt gacattttca gcttgatatg gtaacatgat cgtgaccttcagacagcata 180 aatatgtgtg ccatctcatg tgcaattcct tatagaccca gcttggttcttctccaatgt 240 ctccttttgg agttgt 256 388 566 DNA Homo sapiensmisc_feature (1)...(566) n = A,T,C or G 388 ncnagcggcc gcccngncnggnactgaaca ttggtaaaaa attatatgag ggtaaaacaa 60 aagaagtcta cgaattgttagacagtccag gaaaagtcct cctgcagncc aaggaccaga 120 ttacagcagg aaatgcagctagaaaaaaac cacctggaag gaaaagctgc natctcaaat 180 aaaatcacca gttgtatttttcagttatta caggaagcan gtattaaaac tgccttcacc 240 agaaaatgtg gggagacagctttcattgca ccgcagtgtg aaatgattcc aattgaatgg 300 gtttgcacaa gaatagcnactggttctttt ctnaaaagaa atcctggngt caaggaagga 360 tataagtntt accccctaaagtggagntgt ttttcaagga tgatgcccat taatgaccnc 420 cagtcgggct tgaagaacnacttgattgct gcaaaaattt gcttttcttg gacttcttat 480 anggcnaacc tgaaanggatttcatgaagt catgctacnc aggctatatt tgaaatctgg 540 gagaaatcct ggttgcccaaaattgg 566 389 629 DNA Homo sapiens misc_feature (1)...(629) n = A,T,Cor G 389 actttttttt tttttttttt ttttttgttt tttttttttt ttttttttttttttttttgc 60 agtttctaag tcattacttt tnattttgaa agatttgnga aactnttcacatcatggtga 120 gagtttgtat gattaataan aagcagcttt ttcatgaaat gcttggaggtgaacgagttn 180 tcagcctgng anatccgacc ntcccattaa ctttgaagtt tctcttgattaatagaagaa 240 aaaaggggag ggtgaanaaa aggaggaaca tgctaaaaac cttatgacaatcatccaaat 300 gtgaggaaag aacaacccga ttcaccaact ccactttttc tattttacaactttctacat 360 ctcacncttg gattttggcc ttcntggctn aaacantcct ggcantccnttanagcccct 420 gaaaaagagc cntggntttt ncaaaagacn ntnggnnggn gaannccttnannatgccct 480 gacccnttcn cnaagaactn nntntccggg ntcccaaaag tttgacccancagcttantg 540 tgaannnaaa actnnccttn aaaggtaatg ggnggaanng gtgannaantgggttttttt 600 ganaagtctt ntttttctna aaaccnccg 629 390 596 DNA Homosapiens misc_feature (1)...(596) n = A,T,C or G 390 actttaatttatttcccctt tctagtgtat taagaaatga catgcacttt aatttgccaa 60 aagcaatgcttgtattctgg cagcaacatg ctacttctat cacatagtaa agtgaatacc 120 agaactacaaaggcaggagg tgtaagtgaa tttttattgg gaggggaggt tggcaactta 180 aacagcagcaaataaagagt gaataaggaa actccctgtt gccacagata cacaagacct 240 ccgtatgtgatacaggagcc atttcaattt gtgaccccta gacagagatg gcaagtgctt 300 ttccattcaatctaatactt ccggattcct actaaaaagg aatcattaag agcatggaaa 360 agttgcttactggaaaggaa acccccgaag agtaagggaa gggaatgtga aattaagaag 420 ttatgtggaatctcttaaat tgnaattact acatttctta atttccaggt atnccaaaca 480 cagtccnttgcaaaactggt cagntactta aatnccngat ccattttagg cnttacataa 540 gtgtttgggagtacctatgg tatttnaatg aacttttaaa cttttntccg ccgtcc 596 391 625 DNA Homosapiens misc_feature (1)...(625) n = A,T,C or G 391 acacacccaggaaatttgtc atccaccctg agagtaacaa ccttattatc attgaaacgg 60 accacaatgcctacactgag gccacgaaag ctcagagaaa gcagcagatg gcagaggaaa 120 tggtggaagcagcaggggag gatgagcggg agctggccgc agagatggca gcagcattcc 180 tcaatgaaaacctccctgaa tccatctttg gagctcccaa ggctggcaat gggcagtggg 240 cctctgtgatccgagtgatg aatcccattc aagggaacac actggacctt gtccagctgg 300 aacanaatgaggcagnttta gtgtggctgt gtgcaaggtt tccacactgg tgaagactgg 360 tntgtgctggtgggtgtngn canaggacct ngntnctaaa accncgnntt tgggcaatgg 420 ggctttcgtctaattnttac aannttgntg accaatnggg gatnaactgg anntttttgn 480 tcaanactnttttggaataa tntccctnnt gcnattngcc ntatttcctg gggaanggtg 540 ttnatatngtnatggnnaaa cntntanccg nnntntaatc ttggaatata tatnaatacc 600 ttcttaaaanntgntnatta tcctt 625 392 266 DNA Homo sapiens 392 ggtacccata ttgctaatgctaggatcaag ataccacata gccagaacaa gaagttgaag 60 gtaaacatag aatattttatacaggcactc acacctgcca tttcggaaaa ggattaggaa 120 tccagatgcc gtgaatttaactattcgtta caggcttgtc ctgcaatatg ctctggagca 180 acttgcctgc agagatttctgtatccacgg cttcagagca gaaagagaaa gcaaagaagt 240 agagggagga ataaaaatccccgcgt 266 393 611 DNA Homo sapiens misc_feature (1)...(611) n = A,T,Cor G 393 ggtacttttt tttttttttt tttttttttt tggttttacc tgtttttattccttaaaaga 60 aaaaaacaac ttaaatgcat acatacagaa tagaatacac ttacttaagttttgacagtg 120 aaaaaaaata attacaggtt agatatttaa tccaaggttt aacatggggatgatctcata 180 aggcaatttc tttcctttaa taaatattaa agtgaatatt attctggaagcaaatcatct 240 cctaattctt catcagcaaa atcatcctca tcgatccttt tcttggctgcagtttttggt 300 cgttctattt gagggccaag tgggtccaca taggaggcat ctatttctttgntactgcta 360 ctttcataag gntcatttgt cccaggtaaa agctctgagt ctggccttantccgtcaccc 420 tttactactg gcnctatagt ctggccacta tnaacgntag ccttncttntcnttttgnca 480 cnggagcccc caatgcannt ttngcntgac tttagcncng gnccctaattcttcattttt 540 ccacctttna gnttttggca antcttgagc cntttttaat cnaagacttngcanagccaa 600 ttaaaaaccc c 611 394 340 DNA Homo sapiens 394 acgagtcccactatgcgctg cccctgggcc gcaagaaggg agccaagctg actcctgagg 60 aagaagagattttaaacaaa aaacgatcta aaaaaattca gaagaaatat gatgaaagga 120 aaaagaatgccaaaatcagc agtctcctgg aggagcagtt ccagcagggc aagcttcttg 180 cgtgcatcgcttcaaggccg ggacagtgtg gccgagcaga tggctatgtg ctagagggca 240 aagagttggagttctatctt aggaaaatca aggcccgcaa aggcaaataa atccttgttt 300 tgtcttcacgcaaaaaaaaa aaaaaaaaaa aaaaagtacc 340 395 557 DNA Homo sapiensmisc_feature (1)...(557) n = A,T,C or G 395 acacatcttc aaagcacttccctttaacgg gaaacttagc tttatgggat ttaaacatta 60 gaaagtggga aaaaaaattccattttcttg tcattataaa ccaaaacaaa atctagtgta 120 agtcaaggaa actcattcacacttcaggtc cttctcctcc aggaaccagc attgttatat 180 tatttccatt tagcaaaatctgatgtaatt tagtaatcct tcttccttct ggtgtgattt 240 caaactcagt gacatcttccagtactttnt tttttttttt ttttttttgg gtgttgagct 300 tggacgcttt cttaattggtggctgctttt aggcctacta tgggtgttaa atttttactc 360 tctctacaag gntttttcctagtggccaaa agaagctggt ccctcttttg gactaccgtt 420 aaaattacca nggggatttaaaangggtnt tgngggccaa attnaaagtt ngactangan 480 tctatttttg gccaaccagtnttaaccagg cttcggtang gttggccgcc cccgggtacc 540 ttgggccggg aacacnc 557396 617 DNA Homo sapiens misc_feature (1)...(617) n = A,T,C or G 396ggtacngcgg ggccactcga gtgcgcaggc gcctggcgat taccggtctc accatggagc 60ggaaagtgct tgcgctccag gcccgaaaga aaaggaccaa ggccaagaag gacaaagccc 120aaaggaaatc tgaaactcag caccgaggct ctgctcccca ctctgagagt gatctaccag 180agcaggaaga ggagattctg ggatctgatg atgatgagca agaagatcct aatgattatt 240gtaaaggagg ttatcatctt gtgaaaattg gagatctatt caatgggaga taccatgtga 300tccgaaagtt aggctgggga cacttttcaa cagtatggtt atcatgggat attcagggga 360agaaatttgt ggcaatgaaa gtagttaaaa gtgctgaaca ttacacttga aaccagccta 420gatgaaatcc ggttgcttga agtcagttcc aattcagacc ttatggatcc aaatngaaaa 480atggttgtca actactagat gactttaaaa ttcaggagtt aatggaacac atatttgcat 540gggatttgaa gttttggggc anattngtta agnggttctc aaatcaattn ttangggctt 600tcctgccttg ggtnaaa 617 397 594 DNA Homo sapiens misc_feature (1)...(594)n = A,T,C or G 397 acgcggggga tcaggactcc tcagttcacc ttctcacaatgaggctccct gctcagctcc 60 tggggctgct aatgctctgg gtcccagggt ccagtggggaccgtcgtggt gactcagtct 120 ccggtctccc tgcccgtcac ccttggacag ccggcctccatctcctgcag gtctggtgaa 180 actctccttt acgaagatgg aagcacctac ttgagttggtttcaccagag gccaggccaa 240 tctccgaggc gcctgattta taaagtttct aaccgggactctggggtccc agacagattc 300 agcggcagtg ggtcaggcac ttatttcacg ctgaaaatcaacagggtaga ggctgatgat 360 gttgggaatt attactgcat gccanggtca aactggcccgtcacttttcg gngaaggacn 420 aaaggtggcc natcaaacca actgnggctt gaccattggcttcatnttcc cgccatttga 480 taaccantga aatctggact gctttgtggg ngcctgctgaaaacttntat nccnanaggc 540 cnaagtcatg acagtttttc natttactcg aaaaatntggaaatgataat tttn 594 398 611 DNA Homo sapiens misc_feature (1)...(611) n= A,T,C or G 398 acagtggtcc ttttcagagt tggacttcta gactcacctg ttctcactccctgttttaat 60 tcaacccagc catgcaatgc caaataatag aattgctccc taccagctgaacagggagga 120 gtctgtgcag tttctgacac ttgttgttga acatggctaa atacaatgggtatcgctgag 180 actaagttgt agaaattaac aaatgtgctg cttggttaaa atggctacactcatctgact 240 cattctttat tctattttag ttggtttgta tcttgcctaa ggtgcgtagtccaactcttg 300 gtattaccct cctaatagtc atactagtag tcatactccc tggtgtagtgtattctctaa 360 aagctttaaa tgtctgcatg cagccagcat tcaatagtga atggnctctctttggctgga 420 attaccaaac tcagagaaat gnggcatcag gagaacatct taaccccatgaanggataaa 480 agccccaaat ggngggnact tgataatagc nctaatgctt taaanatttggtccactttt 540 tacctaaggt gagcccattg aaccannggt gctaaangct catacttccaactgaaatgg 600 ttaaggaaaa a 611 399 614 DNA Homo sapiens misc_feature(1)...(614) n = A,T,C or G 399 actctgtgaa tggtgagagg ctgggcacctacatgggcca taccggagct gtgtggtgtg 60 tggacgctga ctgggacacc aagcatgtcctcactggctc agctgacaac agctgtcgtc 120 tctgggactg tgaaacagga aagcagctggcccttctcaa gaccaattcg gctgtccgga 180 cctgcggttt tgactttggg ggcaacatcatcatgttctc cacggacaag canatgggct 240 accagtgctt tgtgagcttt tttgacctgcgggatccgag ccagattgac aacaatgagc 300 cctacatgaa gatcccttgc aatgactctaaaatcaccag tgctgtttgg ggacccctng 360 gggagtgcat catnctggcc atgaaaagtggagagctnaa ccagtattag tgccnnagtt 420 tnnanaaggt gttngttnaa tgttaaaggagcantttccg gnagaataac cnacnttcag 480 gttattccnn gganatgacc anngtttngaccccttnnna gtccattaat nccnaacttt 540 tttacnctca aattttnaan tnanaaaacttttngnatna aattnttnaa ttanttgttc 600 tttttcaata tnnn 614 400 612 DNAHomo sapiens misc_feature (1)...(612) n = A,T,C or G 400 acttacactgtgaaatttta tgatggagta gttcagactg tcaaacatat tcatgtcaaa 60 gctttttccaaagatcaggc ctaaagaaac agatcacaaa agtctttcat catctcctga 120 taaacgagagaagtttaaag aacagagaaa agcaacagtg aatgtgaaga aagacaaaga 180 agataaacccttaaagacag aaaagcgacc caagcagcct gataaagaag gaaagttaat 240 ctgttctgaaaaggggaaag tgtcagagaa aagtcttccc aagaacgaga aggaagacaa 300 ggaaaacatttccgaaaatg acagagagta ttctggagat gcccaagtgg ataagaaacc 360 tgaaaatgacattgtgaaga gtccacaaga aaacttgagg ggaaccnaaa ngaaaacgag 420 gcagacccccttccatagct nctactgctg gggattnaaa ctttaaactt tggcacccat 480 acctttggacttnnnanaag gaaaatttca nagggtgtga agtcctttaa accgtccttg 540 gttgncaaaantttttncng ggaaagtcaa aaacttcttt gaaaaccttg ccnangattt 600 ttnnggngac nt612 401 601 DNA Homo sapiens misc_feature (1)...(601) n = A,T,C or G 401ggtacggtaa ctgactccag ggtcactcat actgtgtccg tggtaacggt aagtctgcag 60ctccatcagg atgggcccct tcccagatct acaataggca gcagcaaacc ttgttgcctc 120tcggacgcac aggatatcca ttccatccac tctcagccca ggaatgaaat cgcctctctt 180gtagtaatca gtgctggctg ccgctctctc aacagacgtt cccattccat agcgattatt 240ctcacagatg aaaatacaag gtaatttcca caaagctgcc atgttgtaag cttcgaatat 300ctggccctgg ttagcagcac catcgccata taaagtcagg cagacctcat cttttccatt 360atacttacag gctagagcaa tcccagcgcc caagggcacc tgcgctccta cgatgccatg 420gccccgtana agtcttggca tacatgtgca tcgatcctcc ttcctttagc acaanctcct 480tttgncctgt aactgcaaaa tttntcggac ggaaaggccc cggtgnaaag taaagccgtg 540agcccggnag gctgngatna aanggcttgt ggggttnaag cccggcttca ggtcccacag 600 a601 402 600 DNA Homo sapiens misc_feature (1)...(600) n = A,T,C or G 402acctggagaa gatcaaacag cgactgtttg agaaccttag aatgctgccg cacgcacctg 60gggtccaaat gcaggcgatt cctgaggacg ccatccctga ggagagtggc cgatgaggac 120gaagacgacc ctgacaagcg catctcgatc tgctcctctg acaaacgaat tgcctgtgag 180gaagagttct ccgattctga agaggaggga gaggggggcc gcaagaactc ttccaacttc 240aaaaaagcca agagagtcaa aacagaggat gaaaaagaga aagacccaga ggagaagaaa 300gaagtcaccg aagaggagaa aaccaaggag gagaagccag aagccaaagg ggtcaaggag 360gaggtcaagt tggcctgaat ggacctnttc agctctggct ttctgctgag tccctacgtt 420ctttcccaac cccttaaatt tataatttct attctctggg gatttatata aaaatttatt 480naatnttaat attcccaggg cccgaaacca agggcccgaa ctnaaggnaa ntttgcttgg 540gtgagctntt tcaagaacca ccttgcaccc atttttccgt cttaacttta accaaaangg 600403 604 DNA Homo sapiens misc_feature (1)...(604) n = A,T,C or G 403actcagtgga tgacgagtgc ttggtgaaat tgttgaaagg cctgtgtctg aaatacctgg 60gccgtgtcca ggaggccgag gagaatttta ggagcatctc tgccaatgaa aagaagatta 120aatatgacca ctacttgatc ccaaacgccc tgctggagct ggccctgctg cttatggagc 180aagacagaaa cgaagaggcc atcaaacttt tggaatctgc caagcaaaac tacaagaatt 240actccatgga gtcaaggaca cactttcgaa tccaggcagc cacactccaa gccaagtctt 300ccctagagaa cagcagcaga tccatggtct catcagtgtc cttgtagctt tgtgcagcag 360ttccgggctg gaagacagag acagctggac agagctcctg aaaacatttc aaaaataccc 420ccttcccctg gcctgccctg cctttggggt ccancggcac ttcagttgga tggcacaacc 480tantgtatcc gtgcnnaaan cnaacctggc attttcaccc anntanccaa gggcttttgc 540caagggnana acagtggagc ccttggcttg ncctataaac atacgggtac cttggccgnn 600acnn 604 404 604 DNA Homo sapiens misc_feature (1)...(604) n = A,T,C orG 404 ggtactttgt ggataagaaa atggaggaac acatctgatg gagagtgggc atttgacaac60 aatggaacag gtaaccagca tgtaaaatca aaatataagt gtctttttaa gagctgaaag 120ctgctgctgg tcattcatta atgtgtcaga catttaatca ggatgctgga ccttcaaaat 180aactgaaaaa agaaccaaga aaaggcgttt ttgttttcaa caaactttac taaataaccc 240cggaaaggca atgaacgatc tgacaattta agctctaatg atttaaagct cagctagaag 300aaagtgaggc atgacatata ctgtcaacgg agggtgaagg aggcagattt ctggaaatgc 360aatgatccca cacatttgct tcaaggagaa acctgcagac atattttcag gtcttgctaa 420gtaacaactg gttatttgta atcaatcatt tgggaaagtc tgctatgtag ctaanggcac 480tgtgaccccn gacaacngat gaaaaggaaa aagcnttgac agcaggaaaa atccttccat 540cttaaagaat ttaggggaca cctttaaagg aaaaaaattg ntccagcctc atttttacaa 600ntnt 604 405 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C orG 405 acttgcattt caaagcttat aagatataaa tggagatttt aaagtagaaa taaatatgta60 ttccatgttt ttaaaagatt actttctact ttgtgtttca cagacattga atatattaaa 120ttattccata ttttcttttc agtgaaaaat tttttaaatg gaagactgtt ctaaaatcac 180ttttttccct aatccaattt ttagagtggc tagtagtttc ttcatttgaa attgtaagca 240tccggtcagt aagaatgccc atccagtttt ctatatttca tagtcaaagc cttgaaagca 300tctacaaatc tcttttttta ggttttgncc atagcatcag ttgatcctta ctaagttttc 360atggggagac ttccttcatc acatcttatg ttgaaatcac tttctgtagt caaaggtata 420ccaaaaccaa tttatcttga actaaattct aaagtatggg tatccaacca tatacatctg 480ggtaccaaac ataaatgctg acattcntat attatagtna aggcttaatc nacttgcagg 540tgaatggaaa aaaaataagc ttnaacctag gattctggaa tgaggaatgc tcn 593 406 591DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 406 actttttttttttttttttt tttttttttg ggactgaatc ttgctctgtc gcccaggctg 60 gagtgcagtggcgcaatctt ggctcactgc aacctctgcc tcctgggttc aagtggttct 120 catgcctcagcctcctgggt agctgggatt acagacaagc accaccacaa ccagctagtt 180 ttttttgttttgtttttttg agacggagtc tcgctctgtc accaggctgg agtgcagtgg 240 cacaatcttggctcactgca acctctgcct cctgggttca agagattctc ctgcttcagc 300 ctnccaagtagctgggacta caggtgcaca ccatcacacc tggctaattt ttgtattttt 360 aagtanagacggggtttcac catgttggcc aggctggtct caaactcctg acctcaagtg 420 aaccggccgcttancctcca aagtgctggg attacaggcg tgagcccact ggcctggctg 480 accatttggttattaacagg gcccccaana tgcnccttta ngtgaaaggg natggcccca 540 gggaacaattnngctgaaaa acaccaaagg ccnantccat aattcnttgg n 591 407 463 DNA Homosapiens misc_feature (1)...(463) n = A,T,C or G 407 ggtactgattttaaaaacta ataacttaaa actgccacac gcaaaaaaga aaaccaaagt 60 ggtccacaaaacattctcct ttccttctga aggttttacg atgcattgtt atcattaacc 120 agtcttttactactaaactt aaatggccaa ttgaaacaaa cagttctgag accgttcttc 180 caccactgattaagagtggg gtggcaggta ttagggataa tattcattta gccttctgag 240 ctttctgggcagacttggtg accttgccag ctccagcagc cttcttgtcc actgctttga 300 tgacacccaccgcaactgtc tgtctcatat cacgaacagc aaagcgaccc aaaggtggat 360 agtctgagaagctctcaaca cacatgggct tgccaggaac catatcaaca atggcagcat 420 caccagacttcaagaattta nggccatctt tcccgggtac ctg 463 408 588 DNA Homo sapiensmisc_feature (1)...(588) n = A,T,C or G 408 acaaatatat ataacttacatttgattgta aggccaacgt tcaaaagtaa aaatgagatg 60 agctctctta ttgttatccgaggtcaagag gctgcaactg tcaaggggat gttctcacca 120 aaagggggtt tgggggaagaggacacacac aaagctaata aaaccagaat ccccatcccc 180 acaaaactca tgggaacaaaatttaaagga taaaacaaaa cccaccaaga cccatattac 240 aaaccaatat ggtaacctgtgttcccttct atggtatgat tatgtcatgt taccttagtg 300 ttaaaagatt aacataaggaaactgcagca atatataaaa gatatattct ctatagagca 360 tatttcgatt gattccattaaaataatgac attagaattc catcatangg ttaaaaccag 420 gacaatactg nttttnctttatttaaaaaa aactaccacc taatgactgn attggtcata 480 acctgaatgg tgtgcaatgggctcttccat gaatggctgg cngaaacaag cttgggncct 540 gcttgagttt cagctttcctctttaattta gtngctcaat gataaaca 588 409 612 DNA Homo sapiens misc_feature(1)...(612) n = A,T,C or G 409 ggtacaaaga tctgacatgt cacccagggacccatttcac ccactgctct gtttggccgc 60 cagtcttttg tctctctctt cagcaatggtgaggcggata ccctttcctc ggggaagaga 120 aatccatggt ttgttgccct tgccaataacaaaaatgttg gaaagtcgag tggcaaagct 180 gttgccattg gcatctttca cgtgaaccacgtcaaaagat ccagggtgcc tctctctgtt 240 ggtgatcaca ccaatttttc taggttagcacctncagtca ccatacacag ggtaccagtg 300 tcnaacttga tgaaaatcaa gtaatcntggccagtctcta aaatcaaatc ttgaatggta 360 tcaattcacc cttgatgaag gggaatcgggggtaacccgg atgggtgccg ggccttnatg 420 aagtcancca natgaaggga ttcctttggggcccccaaag aacttttttn attttcacaa 480 cttgnacctt gcccggcggg ccgttcaaaagggcnaattc cagncacttg gnggccgtct 540 aanggatcca actcggacca acttggcgnaanatggcaaa ctggttcctg gggaaatggt 600 atccctccaa tn 612 410 353 DNA Homosapiens 410 acgcggaagc agtggtaaca acgcagagta acgcgggatg gcacatgcagcacaagtagg 60 tctacaagac gctacttccc ctatcataga agagcttatc acctttcatgatcacgccct 120 cataatcatt ttccttatct gcttcctagt cctgtatgcc cttttcctaacactcacaac 180 aaaactaact aatactaaca tctcagacgc tcaggaaata gaaaccgtctgaactatcct 240 gcccgccatc atcctagtcc tcatcgccct cccatcccta cgcatcctttacataacaga 300 cgaggtcaac gatccctccc ttaccatcaa atcaattggc caccaatggtacc 353 411 612 DNA Homo sapiens misc_feature (1)...(612) n = A,T,C or G411 ggtacgcggg gagagaaacc tggctttact atggcggttg gaggaacggc agtgatcaca 60cgtcggctgc tgggaagatc tggattctcg tttcaggtca ccatcagaaa agctaagttt 120gctgtatagt gaggatcagg agatctgatc ctgattgcag aaccttccct gattacagaa 180tcttgggttg tatctcccac ttcacccttc tagaccatcc cagaagatct ataagatttc 240atctgggaaa tcactaggag ttcttggaag ggaaagaagg aagattgttg gttggaataa 300aaacagggtt gaatgagttc cagaaagcnn ggttctcaac ctcgtggaca gcaatctgca 360gaagangaga acttcaaaaa accnactana agcancttgc anagaagtaa aatgagaagg 420ggncttctna ngaaagaaga cacttggncc acagcagaaa aaactttgac cnantnttnc 480caggaagana gggggggtcc cncttttaaa naaccccctt taagatncng gnggaanacc 540tcanngacca nccntaaatt nnggaaaccg aaaaggggcn gtcctttttg ntnncagntg 600cnccnttaan nt 612 412 607 DNA Homo sapiens misc_feature (1)...(607) n =A,T,C or G 412 acgcggggct ctctcgccag gcgtcctcgt ggaagtgaca tcgtctttaaaccctgcgtg 60 gcaatccctg acgcaccgcc gtgatgccca gggaagacag ggcgacctggaagtccaact 120 acttccttaa gatcatccaa ctattggatg attatccgaa atgtttcattgtgggagcag 180 acaatgtggg ctccaagcag atgcagcaga tccgcatgtc ccttcgcgggaaggctgtgg 240 tgctgatggg caagaacacc atgatgcgca aggccatccg agggcacctggaaaacaacc 300 cagctctgga gaaactgctg cctcatatcc gggggaatgt gggctttgtgttcaccaagg 360 aggacctcac tgagatcagg gacatgttgc tggccaatna ggtgcccagctgctgcccgt 420 gctggtgccc atttgcccat gtgaangtca cttgtgccca gcccaaaacacttgtcttng 480 ggcccganaa gaacttcttt tttccaggcn ttaaaatatt caccccttaaantttcaagg 540 ggccccattt gaaatcctgg annatnngca ttgatcaana ttganacaaagtggnancnt 600 ccaaccc 607 413 606 DNA Homo sapiens misc_feature(1)...(606) n = A,T,C or G 413 acaggtcaga gtcttctttt cttttctttttgagatggag tcttgctctg ttgccagact 60 ggagtgcagt ggtgcgatct gggctcactgcaatctccac ctcccgggtt caagcgattc 120 tcctgcctca gcctcccgag taactgggactacaggtgtg cgccaccaag cccagctcat 180 ttttgtattt ttagtagaga tggggtttcacgatgttggc taggatggtc tcgatctctg 240 gtcagagtct tttctgtaaa tatccttggtaaagaagcaa ttttagactg tagctgttgc 300 aaatgcttta aggaagaagc anaacaactgtcagtcttcc tgaaatgaag aaactacacc 360 agggctgcta tatcagagca accccaaccagcactccaat catgatgccc gacagtggcc 420 ccagcttgag aaccagagaa gttccagatgcagagactgt gagctcntga ctatgggaat 480 tttngnggcn ntaacccaan tttgagacnaaacnaggcct tngncccggt tttnatttgg 540 gngggatttt gcggataaan aaacttgnnggggntnctgc ggnatccatg gaacnccaaa 600 anatng 606 414 624 DNA Homo sapiensmisc_feature (1)...(624) n = A,T,C or G 414 ggtacttttt tttttttttttttttttttg tagatgaggt ctcgctatgt tgcccaggct 60 ggagtgcagt tattcacaggtgcaaccaca gggcactgca gctttaaact cctgggctca 120 agcgatcctc ctgcctcagcctcccaaata gttgggacta gatgcacgca cnaccacgcc 180 tgactcagga cattattcttaaaggtatta tccaggaaac agataaggtc attcataaaa 240 cacacggntt ttttctttagctcagtgtta acaatgaaag tagattccac tattgaagca 300 caagttgcaa attggtaacatagngaacat attgntgtag gaaagggggt tcagtgtgnt 360 gtgttatatn agcncttgaactttttatgg gngtnataag ccnngttatc ttgncccaaa 420 gaaannccat tttnaggattngatggtttt cttannggaa nannctnggg ggnattntgt 480 ngggcatgaa cttttatgtnggaatcagtc ccatanaggt aaggggtttn aatcccaaaa 540 ancggggnct tttatgggaaatnnccttta cttcaaaggc caaanngatn gtnggtgtca 600 cttcnaantt ccngannncaanng 624 415 609 DNA Homo sapiens misc_feature (1)...(609) n = A,T,C orG 415 acgcgggtta caacggaagt aaaatctgtc gaaatgcacc atgaagcttt gagtgaagct60 cttcctgggg acaatgtggg cttcaatgtc aagaatgtgt ctgtcaagga tgttcgtcgt 120ggcaacngtt gctggtgaca gcaaaaatga cccaccaatg gaagcagctg gcttcactgc 180tcaggtgatt atcctgaacc atccaggcca aataagcgcc ggctatgccc ctgtattgga 240ttgccacacg gctcacattg catgcaagtt tgctgagctg aaggaaaaga ttgatcgccg 300ntctggtaaa aagctggaag aaggccctaa attcttgaag tctggtgatg ctgccattgt 360tgatatgggt cctggcaagc ccatgtgttg ttgagagctt tctcagacta tccacctttg 420ggtngctttg ctggtcgtga natgagacag acaggtgccn gtggggtggc atcaanncat 480gggacaanaa aggcttnttg gancttgcaa aggtncncaa nttttgncca naagcntcaa 540aagntaattg aatttttccc ctannncctg cncccncttt tannanggnn ggaaaacggc 600ttaaanntt 609 416 577 DNA Homo sapiens misc_feature (1)...(577) n =A,T,C or G 416 ggtacgagct gattgggaac gggctccaat ggacatggct ctgcagtcaaaatagttagc 60 agatggacag gtttggaaaa tgtgagggcc catatcatca tanccagcaataaggagacc 120 aacaccatat ggtctccggc catatccgtt gtgttggtat ctgggtcttgcttccaatta 180 gagatacaag actgagacac aggcagtggt ctatcgaata caaatctggaatncaaacac 240 tcctgacgca taaaattaca taacagncta gcatnancag taagcccccgcaattgagat 300 accaatatgg ttgtcaacat ggagaatttt tttctgatga cctgccaactcttgatttgc 360 gcccttttca atgcnaaccc aaaactggca tgaagntttt gnatttcagaccancctgnt 420 ggctgnacct tggcttaaca ggtttccatt ggcntatttc natttggatnaantcttgcc 480 cntggggggn ttcnaancta ggggccatca nttggtcaaa ctgntttntaaaccatgggg 540 gcnggctcng gccttggttg ctggcntcaa caaaaan 577 417 570 DNAHomo sapiens misc_feature (1)...(570) n = A,T,C or G 417 ggtactaagaatattagaga actggaaatc cagttttttt gtggtttttt aagaaagaga 60 atctgactccattgcccagc ttggagagca gtggtgcaat agctggggct acaggcgtga 120 gccaccacaccaggcctgga aacccagttt taatttgtga actacaaatg gttggcaact 180 gattccttaattgttattgc aggagtaggc ccaacatgag tccatatgta gtccttctct 240 ggtctggtgggaactgtggg aaatggtgat gaccgtgact tgaaatactn agaaggtgca 300 tgacaaacaaattccaagta ttccatcttc cttggaagat cttcctctgg ccctatgata 360 taggaagcnggaatcaaatt tgggctcttg ggctaagant aggggtatgg aatgagcccc 420 cgtnaantggcttgnacttc ttcttcgcta atactgggcc ctggattaaa accttttgat 480 ttnancnatagntagggctt tccttcttgg ttaatcaatt cccagaaacc aacattccca 540 atttgggtaanatactccct tgtanaaaaa 570 418 570 DNA Homo sapiens misc_feature(1)...(570) n = A,T,C or G 418 ggtacttcta cacatctgcc taacttgggaatgaatgtgg gagaaaatcg ctgctgctga 60 gatggactcc agaagaagaa actgtttctccaggcgactt tgaacccatt ttttggcagt 120 gttcatatta ttaaactagt caaaaatgctaaaataattt gggagaaaat attttttaag 180 tagtgttata gtttcatgtt tatcttttattatgttttgt gaagttgtgt cttttcacta 240 attacctata ctatgccaat atttccttatatctatccat aacatttata ctacatttgt 300 aagagaatat gcacgtgaaa cttaacactttataaggtaa aaatgaggtt tccaagattt 360 aataatctga tncagttctt gntatttcccaatagaatgg gactnngnnc tgttaanggc 420 ttaagganaa agggaagata agggttaaaagttggttaat ggacccaacc ntttnaaaga 480 aatgcnntan anaatanttt natgantaaanaaaggtncc tngcccnggc cggccgtttt 540 aaangggcca atttcnagca cnctnggcgg570 419 574 DNA Homo sapiens misc_feature (1)...(574) n = A,T,C or G 419ggtacacctt tgactacagc tgcagaagtg ttcctttaga caaagttgtg acccatttta 60ctctggataa gggcagaaac ggttcacatt ccattatttg taaagttacc tgctgttagc 120tttcattatt tttgctacac tcattttatt tgnatttaaa tgttttangc aacctaagaa 180caaatgtaaa agtaaagatg caggaaaaat gaattgcttg gtattcatta cttcatgtat 240atcaagcaca gcagtaaaac aaaaacccat gtatttnact tttttttagg attttttgct 300ttctgtgatt tttcttnttt tttgatactt gcctaacatg catgtgctgt anaantnagt 360taaccaggga aataaccttg ngatnatggc ctanctttta gtttangtct tatgaanttt 420tcattgacca attctaanca ataatggttt annaacaccg tgntntnaaa atttctggta 480anttggaaat aaaaggtttn nttgaaatgg gccttttcca cnnactttnt ttnncagctn 540tttcttggna aataagccct nggttcctga aacc 574 420 573 DNA Homo sapiensmisc_feature (1)...(573) n = A,T,C or G 420 acctccggta gaattcggtgaatccatctg gtcctggact ctttttggtt ggtaaactat 60 tgattattgc cacaatttcagctcctgtta ttggtctatt cagagattca acttcttcct 120 ggtttagtct tgggagagtgtatgtgtcga ggaatttatc catttcttct agattttcta 180 gtttatttgc gtagaggtgtttgtagtatt ctctgatggt agtttgtatt tctgtgggat 240 cggtggtgat atcccctttatcatttttta ttgngtctat ttgattcttc tctctttttt 300 tatntagtct tgctagcagtctatcaattt ntgtngatcc ttttcanaaa aacccngctc 360 ctggaattca tttaatnttttnaaggggtt ttttngtggc ctctaatttc cttcaagttc 420 tggctctgat ttaagttaatatncctggct ttttggctac nttttgnaan gnggttggcn 480 cntgnntttt ctanntcctnttnaantggg gatngnttnn aangcccatt ttnggaannt 540 tcccgctttn ntttggggggcatttangtt nnn 573 421 582 DNA Homo sapiens misc_feature (1)...(582) n =A,T,C or G 421 ggtacgcggg ggtccgccat ttcgtggacg ccgggtgagt gagagagttggttggtgttg 60 ggccggagga aagcgggaag actcatcgga gcgtgtggat ttgagccgccgcatttttta 120 accctagatc tcgaaatgca tcgtgatttc tgtccattgg actgtaaggtttatgtaggc 180 aatcttggaa acaatggcaa caagacggaa ttggaacggg cttttggctactatggacca 240 ctccgaagtg tgtgggttgn tagaaaccca cccngctttg cttttgntgaatttgaagat 300 ccccgagatg canctgatgc aatccgagag ctanattngn angaacactatgtggcctgc 360 ccgtgtnagg aattggaact ggccgnaatg gttgaaanaa agaangttcgaaaattcgtg 420 gncctncntt ccttttggng gtcgtcngnc cttnagaatg attaatcgnnnggaaggang 480 tccttccncc ttnncccnan antttncant aaangaanaa agctttttttngcaacccgn 540 aancaggtcc ctttttttag attggganaa atagnngagn tc 582 422570 DNA Homo sapiens misc_feature (1)...(570) n = A,T,C or G 422ggtactctga ggctttagat tcagtttggg tctttggggg ggacctctat catcacgcct 60ataatcatcc cgagagtaat catctctgga gctccacgac cgatcatccc gtctgtcata 120tcggtcttca tagcggtccc cgcctcctct gtagtcatca tccctgcgat acccactgcc 180aaatgctctt ctgccactgc ctatccggga atcatagcct ctatcatagt ctctgctgcc 240tcggtcatca tagcgatccc ggccaccata tcgatccata tcccggcgtg ggccatccga 300tacccatccc gatacccatc ccgataccgg ctgaatcata acgatctcga tacttgnctc 360caaagctatc atcacctctt ctaggtgggt aagtcatcaa agctgtctgg tagcaaggac 420gaagcccttc aagtctggat ctggtttggg cagaatnccc atttttatca cnggccaaaa 480gnaacgaatc atccctnggc tttaaccnng ngcttgatcn agcaacgtcc acntcgaaat 540tntcctngtt acctananaa ctcttcattg 570 423 584 DNA Homo sapiensmisc_feature (1)...(584) n = A,T,C or G 423 acccgggtgg ttaaacttcgcanaatgcct agatattatc ctactgaaga tttgcctnga 60 aagctgttga nccacggcaaaaaacccttc agtcagcacg tgagaaaact gcgagccanc 120 attacccncg ggaccattctgatcatcctc actggacgcc acaggggcan gagggtggtt 180 ttnctgaagc agctggctagtggcttatta cttgtgactg gacctctggt cctnaatcga 240 gttcctctac naagaacacaccaataaatt tgtcattgcc acttcaacca anantcngat 300 atcagcaatg taaaaatnccaaancatctt actgatgctt actttaagaa gangaagctg 360 cngaagccca anacancnnggaaggtgaga tctttcgaca canaagtatg agaanttatg 420 agatttacgg agcaangcanggattgatca nganaagctt ngggcctcac caaatttttn 480 nccaanannt tcaaagtttattttcntnag tttcnnnggg cttncttgcn antctggggn 540 tggctttgnc ctaatgggaatttattnctc ccaaaaatgg nggn 584 424 547 DNA Homo sapiens misc_feature(1)...(547) n = A,T,C or G 424 actcttggtt tgtcaatggg actttccagcaatccaccca agagctcttt atccccaaca 60 tcactgtgaa taatagtgga tcctatacgtgccaagccca taactcagac actggcctca 120 ataggaccac agtcacgacg atcacagtctatgcagagcc acccaaaccc ttcatcacca 180 gcaacaactc caaccccgtg gaggatgaggatgctgtagc cttaacctgt gaacctgaga 240 ttcagaacac aacctacctg tggtgggtaaataatcagag cctcccggtc agtcccaggc 300 tgcagctgtc caatgacaac gggaccctcactctactcag tgtcacaagg aatgatgtag 360 gaccctatga gtgtggaatc cagaacgaattaagtgttga ccacagcgac ccagtcattc 420 tggaatgncc tctatggncc aaacgaaccccaccatttcc cctnatacac taattaccgn 480 ccaggggtga accttaagct tttctggcatgcagccttta cccacctggc acagtattct 540 tggctgn 547 425 567 DNA Homosapiens misc_feature (1)...(567) n = A,T,C or G 425 ggtaccatcctttaatagat ctcatacacc agaattcaga tcatgaatga ctgacagaat 60 attttgttgggcagtcctga tttaaaacta agactggctt gtggttaaat gaatatgttc 120 agtttttgaattttaatagt aactccaatt cagtaaatgg tatcactgtt tacccctttt 180 aaagatatgattagacttcg ttagtaatgt tcaacttttc acaaagatgg tgagtgccat 240 cttaaaacttactggagatt ggctttatat ttagatttat ataactggtt atgtgaatat 300 atttaaatactggggaaatt gcttcactgt cttagaacca agcaagattc acctgtgttt 360 tgtgttcatgttcatttgcc tcttaaaggc aaggggttga agataaataa ggtagcaatg 420 tctatagttttggccttaac ctatgccaat cctaattata attccctgga nttnaaaang 480 gttncttttaccttatttgg aanggcnttt taaatngngg gttnntgggn naatatttaa 540 aggattattcacccctttca catnttn 567 426 563 DNA Homo sapiens misc_feature (1)...(563)n = A,T,C or G 426 ggtacaattt gttcaaggaa tttttgtaga aaaatacgatcctacgatag aagattctta 60 tagaaagcaa gttgaagtag atgcacaaca gtgtatgcttgaaatcttgg atactgcagg 120 aacggagcaa tttacagcaa tgagggattt atacatgaaaaatggacaag gatttgcatt 180 agtttattcc atcacagcac agtccacatt taacgatttacaagacctga gagaacagat 240 tcttcgagtt aaagacactg atgatgttcc aatgattcttgttggtaata agtgtgactt 300 ggaagatgaa agagttgtag ggaaggaaca aggtcaaaatctagcaagac aatggaacaa 360 ctgtgcattc ttagaatctt ctgnaaaatc aaaaataaatggtaatgaga attttttatg 420 acctantgcg gcaaattacc ggaaaaactt ccngngcctgggaaggctng gcaaaaggcc 480 ttcatggtca gntgcttaat tatnctaaat gccntggancttttgaccag gntctgaana 540 actgttgncc aattcaacag ggg 563 427 567 DNA Homosapiens misc_feature (1)...(567) n = A,T,C or G 427 ggtacttttttttttttttt tttttttttt tttttgttaa aaaccataca tcctttttat 60 tgntaagtcataaagaggta tcaaaattaa aagcaaaaat tacagggtaa gacttaacaa 120 aactactaggagcgtcaaag gaagtgaaaa tgggactagg cgcggggcaa tatgaattaa 180 tgaacatgggaaggacaagg atgggganaa cggtgagcat gtgctgaana tactagggga 240 gaggatctggtgaaaaattt gatcttanac aagcgcctag gtaaagaaat aatgggataa 300 gatttctaaaccccactatg gagcttaaga gtcatcctng ccattggcgc tgtctctgnc 360 atcctctccttcctcaagnc tctttttcat catnctttga tccaattcca gctgggcaat 420 tcccccgatctttnattatc atcatcattc cantanggnn cccnttctta ggaanngntn 480 ttttggnccccccttaanat ttcaatttcc cttnnnccca ttttttttan ggagnttgtg 540 gcnntggcccttttnggntt aaaaatn 567 428 578 DNA Homo sapiens misc_feature (1)...(578)n = A,T,C or G 428 ggtaccctat gaacctgact ctgtggtcat ggcagaagctcctcctgggg tagagacaga 60 tcttattgat gttggatnca cagatgatgt gaagaaaggaggccctggaa gaggagggag 120 tggtggcttc acagcaccag ttggtggacc tgatggaacggtgccaatgc ccatgcccat 180 gcccatgcct atgccatctg naaatacngc ctttctcatatccactgcca aagggaccat 240 canatttcaa tggactgcca atggggacct atcaggcctttnccaatatt catccacctt 300 cagataccag cnactccccc atcgnatgaa tctgnanatgacattaatgc tgataatgaa 360 tatctctttn tgcacanatt gttggtcctg gaccccagccaanaancctt tgcaaanctt 420 nctttccaga cctggaggat tacttatnga caccnttgtccctaaccaga agttgnccat 480 ttgngcccng aacancactt tcccaactgg canttngctggatcccagnn ccttcnggat 540 ttggaanaac nttggctttt gatggatttt ttccccgg 578429 572 DNA Homo sapiens misc_feature (1)...(572) n = A,T,C or G 429ggtaccaaga gtttgctcct ggctgctttg atgtcagtgc tgctactcca cctctgcggc 60gaatcagaag cagcaagcaa ctttgactgc tgtcttggat acacagaccg tattcttcat 120cctaaattta ttgtgggctt cacacggcag ctggccaatg aaggctgtga catcaatgct 180atcatctttc acacaaagaa aaagttgtct gtgtgcgcaa atccaaaaca gacttgggtg 240aaatatattg tgcgtctcct cagtaaaaaa gtnaagaaca tgtaaaaact gtggcttttt 300ctggaatgga attggacata gcccangaac agaaagaacc ttgctgggct ggaggtttca 360cttgcacatc atggaagggt ttagtgctta atctaatttg ggcctcactg gacttngncc 420atttaatgaa gttnantcat tattgnnatc atagtttgct ttgtttnaan ccttnncatt 480taaagttaaa actggaattt nanggtaatt tnaacttgta nggtttcctg ggtttagctt 540tttaaatcnt aatttttcca taagcntttt tg 572 430 591 DNA Homo sapiensmisc_feature (1)...(591) n = A,T,C or G 430 ggtacagccc aggtaatttgctgagcctaa tgggtgtcag ggtcagtcta agtgaaggca 60 aagagaggct gggatgaagggtgcaaagga atagtaaaga aagcatgttt gagatccana 120 acagaataat gggtagtagagggaggtatt gaggatagaa nagtatatgg gtttggcacc 180 acggggtgga taggcaaaacatttggttga taangcgcag attctgaact aacttgtaag 240 gcttgtctgg ttttaggacaggtaaaatgg nggaatggta aggagaagtt tataggtttt 300 atgagcccat gctgtancangcaagtgata actngctttt aatccctttt cnaaagcaat 360 gcctggngnt atgaagnatatttggcattt gatcngggtt tnaanggntg attagngttn 420 ctantgaaca atngnaaaggggntgccatg atcngtnncc caaggatgng attttanggn 480 antctcntac ttgtggggttnaagggtggn gggnttttac naggngggtc cccnaagggn 540 gcctnttggn tntangnaataaanggccng nnaatngana atccnnnttn n 591 431 565 DNA Homo sapiensmisc_feature (1)...(565) n = A,T,C or G 431 accagtgatg ttttgatacaagcatataat gtttaatgat caagtcagga taaatggggt 60 atccatcacc tcaagcacatataatcattt ctttgtatta ggcatattca aattccactc 120 ttttagttat ttttaaatatccagtaaatt agatcttatt cattctatct agatgtattt 180 ttgtacttta tttttctcaaatatttttac ttatgctttt tgtcattatc cacagtgttt 240 ttttttaaag cctgagccactttgtggttt cagcctcaat ataataatca tccccttact 300 cttagactaa ttccttttcccctgncactt tgcctgtata ctctgtaaaa atgangacct 360 tagaaaatca acatttcctggtgaactttg agagactatt acaagcagtg cccaaaacag 420 tangaataag gcaggtaaaaccagttggga tagccagatn tattattgat ctggtnggac 480 aaanggataa nttggngggcatggtttcca nggcantcgn gaattcccca ttagctttaa 540 gggtcnatnn angntggcccanggg 565 432 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C orG 432 acgcgggggc caccgtggag agcagagcgc ggcggctgga agctgctaag tcagagccgc60 gatgttccgg attgagggcc tcgcaccgaa gctggacccg gaggagatga aacggaagat 120gcgcgaggat atgatctcct ccatacggaa ctttctcatc tacgtggccc tcctgcgagt 180cactccattt atcttaaaga aattggacag catatgaaga caggacatca catatgaatg 240caccgatatg aagagcctgg ttacagtttc gactcctctc tgnaagtgaa taggcccaga 300aaggtgtaag agactctttg aatggacata aaattctgct tgttnagaac caagttttgg 360ntctgggtna ctgacctttc aaaagctaaa attttaaaac tattttgggg aagtttttta 420tttnnntatt nntcngtttn ttnataaaaa agtaccttgg tnccggnacc acccnttaag 480ggccnaattn cagncnncnt ngngggccgn ttactttnng ggatncntaa nttcggganc 540cnaancttgg ggggtaantc angggtcata nnctggtt 578 433 563 DNA Homo sapiensmisc_feature (1)...(563) n = A,T,C or G 433 acttcttctg gccaaaggctgttccacatt cactacattt aaaaggcttc tctccaatat 60 ggattttctc atgctcagtaaggttggatt tgccactgaa ggtttttcca cactccttac 120 atacaaaggg cttctctcctgtgtgagttc tctggtgtct gatgaggttt gacttctgaa 180 tgaaagcttt cccgcaatctttacactcaa aaggtttttc tccagtgtga attttctggt 240 gcgtaaggag gttttccttctggctaaatg attttccaca ttcattacat tcgaaaagct 300 tctcgccagt atgggtgttctgatgtttaa tgacatactg cttttggcta aaggcttttc 360 cacactcgtt acattcaaaagggttctctc tccgtgtgaa aatgctcatg ctcantgang 420 tttgaattgn nggcttgaagacttttccca tacccttaca ggcaaanggg gttttccccn 480 ttggaanatn tntggctgcntnaagntggt gacatctgga tnggaaacct tttccncatt 540 tccaaaggnn tttttttcnnnag 563 434 563 DNA Homo sapiens misc_feature (1)...(563) n = A,T,C or G434 ggtacagctg tctgcattga aaattcatgc atggagaaag ggagtaagca agggagaaac 60ggtgcgattc acatattccg cgagatcatc aagccagcag agaaatccct ccatgaaaag 120ttaaaacaag ataagcgctt tagcaccttc ctcagcctac ttgaagctgc agacttgaaa 180gagctcctga cacaacctgg agactggaca ttatttgtgc caaccaatga tgcttttaag 240ggaatgacta gtgaagaaaa agaaattctg atcgggacaa aaatgctctt caaaacatca 300ttctttatca cctgacacca ggagttttca ttggaaaagg atttgaacct ggtgttacta 360acattttaaa gaccacacaa ggaaacaaaa tcttttcttg aaagaaagta aatngatcca 420cttctggtga atgaatttga aattcaaagg aatctggcct tcatgccanc aaatgggggt 480aattcatgnt ggagaataac ctcctttatc cagccgnaca cacctgttgg aaatggatcc 540aactgctgga aattncttaa taa 563 435 558 DNA Homo sapiens misc_feature(1)...(558) n = A,T,C or G 435 ggtacgcggg ggaagatggc ggccgtgcaggcggccgagg tgaaagtgga tggcagcgag 60 ccgaaactga gcaagaatga gctgaagagacgcctgaaag ctgagaagaa agtagcagag 120 aaggaggcca aacagaaaga gctcagtgagaaacagctaa gccaagccac tgctgctgcc 180 accaaccaca ccactgataa tggtgtgggtcctgaggaag agagcgtgga cccaaatcaa 240 tactacaaaa tccgcagtca agcaattcatcagctgaagg tcaatgggga agacccatac 300 ccacacaagt tccatgtaga catctcactcactgacttca tccaaaaata taagtcacct 360 gcagcctggg gatcacctga ctgacatcaccttaaaggtg gcaggtagga tccttccaaa 420 agancttntg ggggaaactn antcttctnttgaactttca aggaaanggg tgaagtttgc 480 agtcatgggc caattccaga aattttaaatcagnagaaga atttttccta ttaataccaa 540 ctgggtcggg ggagactn 558 436 528 DNAHomo sapiens misc_feature (1)...(528) n = A,T,C or G 436 ggtacaaaaaaaaccttaca taaattaaga atgaatacat ttacaggcgt aaatgcaaac 60 cgcttccaactcaaagcaag taacagccca cgatgttctg gccaaagaca tcagctaaga 120 aaggaaactgggtcctacgg cttggacttt ccaaccctga cagacccgca agacaaaaca 180 actggttcttgccagcctct agagaaatcc cagaacactc agccctgaca cgttaatacc 240 aaggggaacagttaactcca atacaaggtc aaaatcagca acaagttcta caatccagtg 300 ctgatatcagatacaaagct tcaagggcaa tttcttttcg aaggcttatt ccagtttcgt 360 gaggctagcatgaagtgtgt gcatttgcca ggggcaaatt tctattctca attaacccat 420 gcagcaaantgctacgcatc tggctgagtc cggtttanaa nccatttgcc ggnggaccaa 480 tggaaggggcccgaattcgt cnnaacttgn cccgggcggg ccgttcaa 528 437 576 DNA Homo sapiensmisc_feature (1)...(576) n = A,T,C or G 437 actttttttt tttttttttttttttttttt aggtttgagg gggaatgctg ganattgtaa 60 tgggtatgga gacatgtcatataagtaatg ctagggtgag tggtaggaag ttttttcata 120 ggaggtgtat gggttggtcgtagcggaatc gggggtatgc tgttcgaatt cataagaaca 180 gggaggttag aantagggtcttggtgacaa aatatgttgt gtagagttca gggganagtg 240 cgtcatangt tgttcctaggaanattgtac nggtgagggt tgtttattat aataatgttn 300 gggtatccgg ctntgaaanaatngggccaa ngggcctgcg gtgtattcga ngttnaaacc 360 tgagactagt tcggactcccntttgcaagg ncccaaaggg ggttnggttt ggcccttgct 420 annggtgnga naataaatcntntttattgg cccaagggtt cttaacngcn aggagtnaat 480 ccaaaggggt ncntnggnttttnnnanaaa nggttgnnaa aaggttaaag ggacccncct 540 ttntnnntaa tgntcgnaatgtcaaatnga tngcnn 576 438 576 DNA Homo sapiens misc_feature (1)...(576)n = A,T,C or G 438 ggtaccccaa ttaccagtat ggtggaccct accccttcttctctgcattg ggaaacagaa 60 cagagaacag aaaaaatcat tccatcttgc tcttaactctttccacctat gtgctcagtt 120 tttcaagtag aatttctatt cctttgctgg tgcttttggttttttccaat gtaggaatca 180 agcttttcag tgcagctttg actttgtttg caacttccaggtcacaactc tggaggaggc 240 tagaaagaat aatggcacct cgatttacac tagcccaggacttcaggttc ttcataccaa 300 catgctctac aagtgttttt gcaaaacaac cttctcttccattntctttt catcttttta 360 tcttgctcta ttaaccactt nagaaactaa gaatgtccctgcaaggatgt tctggcaatg 420 ntgaaagctt ctccgtcctt ggccaccagg atgcaagtccntggttnttg ccagcttggc 480 cnatnggcat tccatnggna nggcttgaac cgttttccagggggcagant cccaaaatgg 540 ccngacacca acccnacang cagacttntt ttagcn 576439 578 DNA Homo sapiens misc_feature (1)...(578) n = A,T,C or G 439cgaggtacgc gggggagaaa aaacctgcgg aaaatggtag cgatggcggc tgggccgagt 60gggtgtctgg tgccggcgtt tgggctacgg ttgttgttgg cgactgtgct tcaagcggtg 120tctgcttttg gggcagagtt ttcatcggag gcatgcagag agttaggctt ttctagcaac 180ttgctttgca gctcttgtga tcttctcgga cagttcaacc tgcttcagct ggatcctgat 240tgcagaggat gctgtcagga ggaagcacaa tttgaaacca aaaagctgta tgcaggagct 300attcttgaag tttgnggatg aaaattggga aggttccctn aagtccaanc ttttgttang 360agtgataaaa cccaaactgt tcagaaggac tgccaaatna aagtatgtnn cgtggtttca 420aaccntgaat taaaaggctt ttngaccaac atngggnaca attgcttgan nacttgtcca 480tttcttaaaa ttgggaacnc tggaccnggt nanaaanatt tcngattgga aaantttgga 540ccncatttta aatcttgctt aaattttggc caatcctt 578 440 573 DNA Homo sapiensmisc_feature (1)...(573) n = A,T,C or G 440 ggtacttttt tttttttttgagacagggtc ttgccctgtc acccaggctc gagtgcactg 60 gagtgatcac agctcactggcctcaagtga tcctcctgcc ttggcccctt aagtgccagg 120 gttacaggca tgagctaccatgcctggcag aaattcaaga tttggataaa cttacttctt 180 tgccaagcct gttcttcaagttattcagaa ctgggtgtat accttgtcct catatgtatc 240 ttgtccctgc tgtcttttaggttagcaagg tgtatgaata cttttaagtt ttgtttgttc 300 ttttcctcgt ggtatcaagtgaaatactga tctattctct ggctagggtc aatttacaaa 360 attgccatgg aactgagccaaaaggcccca cgtgggataa aaattnctta ccatcgacgc 420 ccanccgtan tttttcaaggtattggcttt tggaagnttt accaaatttc nggtaaacca 480 aaattcnaaa agnaaaaaattnccctggng taaccttgcc cgggcggccg ttcaaaaggg 540 cnaatttcca ncacattgggcggccgttaa tna 573 441 572 DNA Homo sapiens misc_feature (1)...(572) n =A,T,C or G 441 ggtacaaaat tttattaaag gtctttagag agcaacatcc agactccagaatacagctgc 60 caaggagacc ctgttatgct gtggggactg gctggggcat ggcaggcggctctggcttcc 120 cacccttctg ttctgagatg ggggtggtgg gcagtatctc atctttgggttccacaatgc 180 tcacgtggtc aggcaggggc ttcttagggc caatcttacc agttgggtcccagggcagca 240 tgatcttcac cttgatgccc agcacaccct gtctgagcaa cacgtggcgcacagcagtgt 300 caacgtagta gttaacaggg gtctccgctt gtggatcatc aagccatccacaaacttcat 360 ggatttagcc ctctgncctt cggaggttcc cagacaccca caanctngcaagcctttggc 420 cccacttttc catgatgaaa ctgnagncac aaccatangc aagggcccttcggacannta 480 aggccttcct aaggagnttg naacncnana naacttttgc ttgggcantgggcacaccag 540 nacctntaag nggccccctt tttaagcata aa 572 442 562 DNA Homosapiens misc_feature (1)...(562) n = A,T,C or G 442 acaggtcagagtcttctttt cttttctttt tgagatggag tcttgctctg ttgccagact 60 ggagtgcagtggtgcgatct gggctcactg caatctccac ctcccgggtt caagcgattc 120 tcctgcctcagcctcccgag taactgggac tacaggtgcg cgccaccaag cccagctcat 180 ttttgtatttttagtagaga tggggtttca cgatgttggc taggatggtc tcgatctctg 240 gtcagagtcttttctgtaaa tatccttggt aaagaagcaa ttttagactg tagctgttgc 300 aaatgctttaaggaagaagc aaaacaactg tcaagtcttc ctgaaatgaa gaaactncac 360 cagggctgctatatcagaac aaccncaacc aagcacttca aacatgatgc cgacaggtgg 420 ccccagcttaaaaaaccagg aanaagttcn gantcccnaa actgngaatg cctcttggac 480 ttttggaattaattgggggc cagtagccaa gttatnagac caaatcangg cntagggccc 540 cgtattatttggcggggatt tg 562 443 585 DNA Homo sapiens misc_feature (1)...(585) n =A,T,C or G 443 acttttattt tttggtggtg aaattgactg atgattttcc tttttcttcgctggactatt 60 gtgccaactg ccaggctgcc tcctgccctt acagccctaa gtggctgccttctttccatc 120 aactcccaac ttcttcctgt gaagtttaat tgtctcaacg cctccccctcccccattccc 180 tccatttttc tcccaagaaa cctgactcaa ttatttgcat attttgagaaactgctgcag 240 attagttctt tttgccagtt ttccctggaa ctcctggcct tttgtggaggggagggatgg 300 agagaatagg aatcttcact agaagccgtg ggaagaattg gaagttacatgctgtatatg 360 caatgtccag cagtctgata aactgacgat tcttaatcaa gattttttcctgatggggaa 420 gggactttta ttttctttta nagaggggaa agtgtgagct cttcccttattcctaatggc 480 tatttttgaa gcaaanaagg ccacaacatt ngcacatgcc acctgcnaaggaccttgagt 540 nagtgaagnc tcctaaaact gggttaanaa ccttgttttc tctnn 585 444437 DNA Homo sapiens misc_feature (1)...(437) n = A,T,C or G 444acgcggggac gtgactcagc actttcccca gagcccggac tgcggagaac aatatcctcc 60tccctaacag ataaacagcc cttgttcctc gggataagga ctggcagtcc cctgacaccc 120taagaccggc atctgtcgat gttatttccc cagcatggcc gaaacagaag ccctgtcgaa 180gcttcgggaa gacttcagga tgcagaataa atccgtcttt attttgggcg ccagcggaga 240aaccggcaga gtgctcttaa aggaaatcct ggagcagggc ctgttttcca aagtcacgct 300cattggccgg aggaagctca ccttcgacga ggaagcttat aaaaatgtga atcaagaagt 360ggtggacttt gaaaagttgg atgactacgc ctctgccttt caaggtcatg atgttggatt 420ctgtgcctgg gtacctn 437 445 592 DNA Homo sapiens misc_feature (1)...(592)n = A,T,C or G 445 actttttttt tttttttttt tttttttttt taaggtttgagggggaatgc tggagattgt 60 aatgggtatg gagacatatc atataagtaa tgctagggtgagtggtagga agttttttca 120 taggaggtgt atganttggn cgtagcggaa tcgggggtatgctgttcgaa ttcataagaa 180 cagggaggtt aaaagtaggg tcttggtgac aaaatatgttgtgtanagtt caggggaaag 240 tgcgtcatat gttgttccta ggaanattgt antggtgagggtgttaatta taataatgtt 300 tgtgtattcg gctatnaana atagggccaa atgggcctgcngcctattcn atgtttaanc 360 tgagacttnt tcggactccc cttcggcaan gtcnaantggggttcggttg ngcnctgcag 420 tgnggagata nntcntntta ntggccaatg gtnnngatggccagaataat cannanggnt 480 tcnttnntcn tnaaaaggtc naaatggttn angganaccncttattagga attgttaatc 540 ttnaatgatn gttntggnga cnctatatgg anaatgtnaggnctactccn ng 592 446 599 DNA Homo sapiens misc_feature (1)...(599) n =A,T,C or G 446 ggtacggcaa acacaacgga cctgagcact ggcataagga cttccccattgccaagggag 60 agcgccagtc ccctgttgac atcgacactc atacagccaa gtatgacccttccctgaagc 120 ccctgtctgt ttcctatgat caagcaactt ccctgaggat cctcaacaatggtcatgctt 180 tcaacgtgga gtttgatgac tctcaggaca aagcagtgct caagggaggacccctggatg 240 gcacttacag attgattcag tttcactttc actggggttc acttgatggacaaggttcat 300 agcatactgt ggataaaaag aaatatgctg cagaacttca cttggttcactggaacacca 360 aatatgggga ttttgggaaa gctgtgcagc aacctgatgg actggccgttctaggtattt 420 tttttgaagg ttggcagcgc taaaccnggc cttnataaag ttgttgaatgtgctggattc 480 cattaaaaca aagggcaaga attgctgact ttcactaatt nnaatcctcgtnggccttct 540 tcctgaaatc cttggattac cggacctncc cagcttactn accanccttctcttttngg 599 447 588 DNA Homo sapiens misc_feature (1)...(588) n =A,T,C or G 447 ggtacgcggg atgagtgtgg aatccagaac aaattaagtg ttgaccacagcgacccagtc 60 atcctgaatg tcctctatgg cccagacgac cccaccattt ccccctcatacacctattac 120 cgtccagggg tgaacctcag cctctcctgc catgcagcct ctaacccacctgcacagtat 180 tcttggctga ttgatgggaa catccagcaa cacacacaag agctctttatctccaacatc 240 actgagaaga acagcggact ctatacctgc caggccaata actcagccagtggccacagc 300 aggactacag tcaagacaat cacagtctct gcggagctgc caagccctccatctccagca 360 acaactccaa acccgtggag gacaaggatg ctgtggcctt ccctgtgaacctgaggctca 420 gaacacaacc tacctgtggt gggtaaatgg tcagagcctc cagcagtcccaaggctggag 480 ctgtccaatg gcaacangga cctnactcta ttcaatgtca caagaaatgacncaagaacc 540 tatgnatgtg gaatccagaa ctnagtgatg caaaccgaat gaccagnn 588448 593 DNA Homo sapiens misc_feature (1)...(593) n = A,T,C or G 448accatttgtc tgacctctgt aaaaaatgtg atcctacaga agtggagctg gataatcaga 60tagttactgc tacccagagc aatatctgtg atgaagacag tgctacagag acctgctaca 120cttatgacag aaacaagtgc tacacagctg tggtcccact cgtatatggt ggtgagacca 180aaatggtgga aacagcctta accccagatg cctgctatcc tgactaattt aagtcattgc 240tgactgcata gctctttttc ttgagaggct ctccattttg attcanaaag ttagcatatt 300tattaccaat gaatttgaaa ccagggcttt tttttttttt ttgggtgatg taaaacncaa 360ctncctgnca ncaaaataat taaaatagnc acattgntat cttttattag gtaattcact 420tcttaattan atggntcaat actctaagna tcaaaatntt ccaattatna tggctcacct 480gaaagaagna tgctctttta aggaatacag cttcttcnat tnacaattta acanggggag 540aaaattaaan tnaangantt ganatctgga ggngtannaa ngntctcgcn ttc 593 449 577DNA Homo sapiens misc_feature (1)...(577) n = A,T,C or G 449 actgtgggtcgaagtaatgg atacggacgt aaccatcttc gccgccgctg ctgtagctct 60 tgccatcaggatggaaggca acactgttga taggtccaaa gtgacccttg actcttccaa 120 actcttcttcaaaggccaaa tggaagaacc tggcctcaaa cttgccaatc ctggtggagg 180 ttgtggttacatccatggct tcctgaccac cgcccaggac cacatggtca tagttggggg 240 agagggcagctgagttgaca ggacgttctg tccggaaagt cttctgatgt tcaagagttg 300 tggagtcaaaaagcttggct gtgttgtcct tggacncggc acaaacatgg tcatgtccct 360 ggataactggatgtcgttga tctgccggga gtgctcctta acattcacca acacctcttc 420 anacttggcactatactggt tgactctcca ctcttatggc cnggatgatg cactccccca 480 aggggtnccaaacagnactg gtgatttaga atcattgcan ggatcttatg tagggctcat 540 tgntgcaatctggcttggat ccgcagtcaa aaaagnt 577 450 575 DNA Homo sapiens misc_feature(1)...(575) n = A,T,C or G 450 ggtacttgtg atcacactac gggaatctctgtggtatata cctggggcca ttctaggctc 60 tttcaagtga cttttggaaa tcaaccttttttatttgggg gggaggatgg ggaaaagagc 120 tgagagttta tgctgaaatg gatttatagaatatttgtaa atctattttt agtgtttgtt 180 cgttttttta actgttcatt cctttgtgcagagtgtatat ctctgcctgg gcaagagtgt 240 ggaggtgccg aggtgtcttc attctctcgcacatttccac agcacctgct aagtttgtat 300 ttaatggttt ttgtttttgt ttttgtttgtttcttgaaaa tgagagaaga gccggagaga 360 tgatttttat taattnttnt tttttttttttactatttat agctttaaaa agggcctncc 420 ttcccctctt ctttctttgg nctctttcattaaccccttc ccagtttttt ttaacttaaa 480 ccccgttctc atggcctngg ccttttgaagcgnttcctct tataaaaagc tttgccgaac 540 aanttttttt taccgatccc aaatttatgaagggg 575 451 573 DNA Homo sapiens misc_feature (1)...(573) n = A,T,C orG 451 actaggctaa ctagaaggat ctcatcccca tatgtggtct catttcaagt ctatggatga60 ctaccttcat tgctgtgtgc gagatggttt caccccttga aaatatggtc acttcagcat 120aaaatagtta aatctttata atgatcaatt catcctacct ccttttacat gcagctgaaa 180aatgacaggc tagggacata gaatattgtg aactttatac tgttagaatc actgtccatt 240aaatgatcac tagctaatgg tcactaaatt tacaaattaa ggaaattata tatagaatac 300tgcaaaaaca cagtaaaaag actgaagttc gcccatttct gctcaggaag tctcttcact 360cctaagcttc atatgttgcc ttctggcttc aaaattctgc tattattact gttttcctcc 420tttgatcttc ctttggtccc cagtgccaga cttccaagcc ttttngttaa aaagccatct 480tttggatgcc atttcnaaca gcttcagtga tgcctctgaa aaaaggatct gccggctaan 540atttctcngg ttcgtgcttt ctaccgganc tcc 573 452 595 DNA Homo sapiensmisc_feature (1)...(595) n = A,T,C or G 452 acaattttat ccctaaaactctgttgacat caaaatatga cagttgctat atccataaaa 60 tatttacata gcacggcatattaagcttta gacacttggc aattaaacca cataaaaaga 120 ggacaagacc cccatcctacatgtttggaa tcaggtgttc accggtccct atctggcgac 180 tgtacgcggg tggggtccttacttgtattc tgttatcagc tgattttgaa acatataata 240 atgattttct tgttcccttctttaactagc tgcctttaga ttttgataat cacagtctta 300 aaatactagg aaagaagtggatgggaattg taggcataga tttcatatca agggcatttc 360 aagacagaat ttttaattcctgtagtaggc ttgctggagc naaaggaaaa tgtgctggtt 420 aaaaatcaac ttatgccattttaaaatttg ataaaatttg gagtggcatn ctgctaaggg 480 gagaccttgg gccggacccccttangggca aattccngca cactgggggg cggtactang 540 gggatccgac ntcggnccanacttggcgna tcatgggctt antgttcctt gnggn 595 453 380 DNA Homo sapiensmisc_feature (1)...(380) n = A,T,C or G 453 ggtacgcggg gagccgcctggataccgcag ctaggaataa tggaatagga ccgcggttct 60 attttgttgg ttttcggaactgaggccatg attaagaggg acggccgggg gcattcgtat 120 tgcgccgcta gaggtgaaattcttggaccg gcgcaagacg gaccagagcg aaagcatttg 180 ccaagaatgt tttcattaatcaagaacgaa agtcggaggt tcgaagacga tcagataccg 240 tcgtagttcc gaccataaacgatgccgacc ggcgatgcgg cggcgttatt cccatgaccc 300 gccgggcagc ttccgggaaaccaaagtctt tgggttccgg ggggagtatg gttgcaaaaa 360 aaaaaannaa aaaaaaaagt380 454 589 DNA Homo sapiens misc_feature (1)...(589) n = A,T,C or G 454ggtactcttg gtttatcaat gggacgttcc agcaatccac acaagagctc tttatcccca 60acatcactgt gaataatagc ggatcctata tgtgccaagc ccataactca gccactggcc 120tcaataggac cacagtcacg atgatcacag tctctggaag tgctcctgtc ctctcagctg 180tggccaccgt cggcatcacg attggagtgc tggccagggt ggctctgata tagcagccct 240ggtgtatttt cgatatttca ggaagactgg cagattggac cagaccctga attcttctag 300ctcctccaat cccattttat cccatggaac cactaaaaac aaggtctgct ctgctcctga 360agccctatat gctggagatg gacaactcaa tgaaaattta aagggaaaac cctcaggcct 420gangtgtgtg ccactcagag acttcaccta actagagaca gtcaaactgc aaccatgggt 480gagaaattga cgacttcaca ctatggacag cttttnccaa gatgtcaaac aagactcctc 540atcatgataa ggntcttacc cctttaattg nccttgttat gcctgccct 589 455 589 DNAHomo sapiens misc_feature (1)...(589) n = A,T,C or G 455 ggtacgcggaagagacaggg tttcaccatg ttgcccaggc tggtttcgaa ctcctgacct 60 caggtgatccacccgcctcg gcctcccaaa gtgctgggat tacaggcttg agcccccgcg 120 cccagccatcaaaatgcttt ttatttctgc atatgttgaa tactttttac aattcaaaaa 180 aatgatctgttttgaaggca aaattgcaaa tcttgaaatt aagaaggcaa aaatgtaaag 240 gagtcaaaactataaatcaa gtatttggga agtgaagact ggaagctaat ttgcattaaa 300 ttcacaaacttttatactct ttctgtatat acattttttt tctttaaaaa acaactatgg 360 atcagaatagccacatttag aacacttttt gttatcaagt caatattttt agatagttag 420 aacctggtcttaagcctaaa agtgggcttg attctgcagt aaatcnttta caactgcctc 480 gacacacattaaccttttta aaaatngacc ttcccgaagt cttttggtag catggnacac 540 ctgatgcttanatgttcang taattaatat ggnccagnag tnttgtnnc 589 456 582 DNA Homo sapiensmisc_feature (1)...(582) n = A,T,C or G 456 acagaatgtt gatacaaagcttaaaattct tgcatatggt catagaaaat gcatctttgg 60 ttttgtgttt ttatcacttgcttccaactt aggcttttgg ctcagaagat tattgaataa 120 tgatttgtct tagtttctgtttcagtaagg gaattctgag gccgttgcta tgataccatc 180 attaagacat tcacatgtcttcatataata tctcttcatt tcaaatccta atcactattt 240 catactatta cagggctttgatgctgccag cactgtcttt tacataggaa attctagatt 300 tgcacagtaa tagaggaattagaagtacct aactatacac tttgattcag cctgctaaat 360 caggggttca atactagcttggacaaactt tgtaagtaat taattgctac cagccttatt 420 ggaaacaaat tatcaactagtttcccctgc caaattttga aattcactgn ttcacttaat 480 ctattatatt actaataatggattaataaa gatgaattaa ttattattac ttactagtnt 540 aaatgaaaaa cagggactgaaatagtctgn atccgngttg ca 582 457 380 DNA Homo sapiens 457 ggtacttttttttttttttt tttttggagt ttttagttta ttaatgttct tgcgaaaaat 60 ccacagtggccacagctaac atcattgcag cacctttact ccttcggctg tgatccaatc 120 tccagctcacttctttttgc cagcaccaac attggccttt gcagtccccc tgactttctt 180 cattctgttcttgcgttcct ttcgttgctt tcttgaggtc tttttcttct catacaggcc 240 atgtcttgcaagtctatgtt tgggttcatt tttctttgca taatccaggg aatcataaat 300 catgccaaagccagttgtct tgccaccacc aaaatgagtt ctgaatccaa atacaaagat 360 gacatccggtgtggtcttgt 380 458 382 DNA Homo sapiens misc_feature (1)...(382) n =A,T,C or G 458 acgcggggag aacagccacc cctctctcgg gcactgctgc catgaatgccttcctgctct 60 ccgcactgtg cctccttggg gcctgggccg ccttggcagg aggggtcaccgtgcaggatg 120 gaaatttctc cttttctctg gagtcagtga agaagctcaa agacctccaggagccccagg 180 agcccagggt tgggaaactc aggaactttg cacccatccc tggtgaacctgtggttccca 240 tcctctgtag caacccgaac tttccagaag aactcaagcc tctctgcaaggagcccaatg 300 cccaggagat acttcagagg ctggaggaaa tcgctgagga cccgggcacatgtgaaatct 360 gtgcctacgc tgcctgtacc tn 382 459 592 DNA Homo sapiensmisc_feature (1)...(592) n = A,T,C or G 459 ggtactgagg aaatattttgtaaagtgagc tttgggtata acttagcccc atcattattt 60 agagaataga ggaggaagaaagaggaagga ttttaaaggc agacaatgac agaccattca 120 ggataggtag ggttttaaagggagataaac acagtctcat caactaagga gagatttgct 180 gcagtaaata ggatgagggaaatagtctgt gggatgcaag caaaggaagc agggtgcctt 240 agacactgag tggagccagaaagatcatgc ggcctttttc caagtacatg gccaccaagt 300 aagaatggtt ggtgacaagacagaaggcta aaacaggaag gtaatcttgt gcacctgaca 360 aatngaaaga attaaggatcaaaattgaag caggctntaa gagtttcaag aaattcttaa 420 aacccaaaag tgatttggaagccccaaact ttccggtaat gctncccatg gcatgatggg 480 ccaaaacctt gggggttcctaagttnnaaa agccctntnc caaattttaa tggaccccct 540 acattttttc taatcaatcccccctttcca aaaaaatngg acctcntttt tt 592 460 578 DNA Homo sapiensmisc_feature (1)...(578) n = A,T,C or G 460 acgcgggcac tatcctgaattatgtgcctg tctagataag cagagaccat gccaaagcta 60 taatggaaaa caagtttacaaagagacctg tatttctttc ataaaagact tcttggcaaa 120 aaatttgatt atagttattggaatagcatt tggactggca gttattgaga tactgggttt 180 ggtgttttct atggtcctgtattgccagat cgggaacaaa tgaatctgtg gatgcatcaa 240 gctatcgtca gtcaaacccctttaaaatgt tgctttggct ttgtaaattt aaatatgtaa 300 gtgctatata agtcaggagcagctgtcttt ttaaaatgtc tcggctagct agaccacaga 360 tatcttctag acatattgaacacatttaag atttgaggga tataagggaa aatgatatga 420 atgtgtattt ttactcaaaataaaagtaac tgttacgttg cgaaaaaaan nnnnnnnnnn 480 naaaaaaaag tnccttgggccgggaccacg ctagggcaaa tccagcacac tggcggccgt 540 actagggatc cactnggaccagctggcgna atatggnn 578 461 425 DNA Homo sapiens misc_feature(1)...(425) n = A,T,C or G 461 acgcggggct ttctggtctc ggccgcagaagcgagatgac gaagggaacg tcatcgtttg 60 gaaagcgtcg caataagacg cacacgttgtgccgccgctg tggctctaag gcctaccacc 120 ttcagaagtc gacctgtggc aaatgtggctaccctgccaa gcgcaagaga aagtataact 180 ggagtgccaa ggctaaaaga cgaaataccaccggaactgg tcgaatgagg cacctaaaaa 240 ttgtataccg cagattcagg catggattccgtgaaggaac aacacctaaa cccaagaggg 300 cagctgttgc agcatccagt tcatcttaagaatgtcaacg attagtcatg caataaatgt 360 tctggtttta aaaaatnnan nnnaannntnntnnaaanaa aaaaagtnct nggccgngac 420 cacgc 425 462 581 DNA Homo sapiensmisc_feature (1)...(581) n = A,T,C or G 462 ggtactattg acccagcgatgggggcttcg acatgggctt tagggagtca taagtggagt 60 ccgtaaagag gtatctttactataaaagct attgtgtaag ctagtcatat taagttgttg 120 gctcaggagt ttgatagttcttgggcagtg agagtgagta gtagaatgtt tagtgagcct 180 agggtgttgt gagtgtaaattagtgcgatg agtaggggaa gggagcctac tagggtgtag 240 aataggaagt atgtgcctgcgttcaggcgt tctggctggt tgcctcatcg ggtgatgata 300 gccaaggtgg ggataagtgtggtttcgaag aagatataaa atatgattag ttctgtggct 360 gtgaatgtta taattaaggagatttgtaag ggagattagt atanagaggt anagtttttt 420 tcgtgatagt ggntcactggataantggcc gttggctttg ccatgattgt gaggggtagg 480 agtcaagtag ttagtattangangggggtt nttaggggtc cnaggaaang ttggggaana 540 ctaaannggt gtngtnattngtaaaaaata nnnnanggat n 581 463 574 DNA Homo sapiens misc_feature(1)...(574) n = A,T,C or G 463 actgtgtggc gccttattct aggcacttgttgggcagaat gtcacacctg ccgatgaaac 60 tcctgcgtaa gaagatcgag aagcggaacctcaaattgcg gcagcggaac ctaaagtttc 120 agggggcctc aaatctgacc ctatcggaaactcaaaatgg agatgtatct gaagaaacaa 180 tgggaagtag aaaggttaaa aaatcaaaacaaaagcccat gaatgtgggc ttatcagaaa 240 ctcaaaatgg aggcatgtct caagaagcagtgggaaatat aaaagttaca aagtctcccc 300 agaaatccac tgtattaagc aatggagaagcagcaatgca gtcttccaat tcagaaccaa 360 aaaaaaaaaa naaaaaaaag tactttttttttttnnnnnt tttttttttt taggtaatgg 420 gtgttgagct tgaacgcttt cttaattggnggctgctttt angcctctat gggtgttaaa 480 ttttttactc tcttacaagg tttttcctaagtccaaanac tgtccttttg gctacagtta 540 aatttccagg ggattaaagg gttttgggcnaatt 574 464 580 DNA Homo sapiens misc_feature (1)...(580) n = A,T,C orG 464 ggtacctagt aagctctccc tcctcccacc ctccaccctc aaggaggccc cagtgtcagt60 tgttcccctc tgggtccatg agttcttatc atttagctcc cacttataag caagaacatg 120cagtatttgg ttttctgttc ctgccttagt ttgctaagga taacggcctc cagctccatc 180cagttcctgc aaaggacatg atcctgttct ttctatggct gtatagtatt ccatggtgta 240tatttaccac attgtcttta tccagtctgt cattgatggg cttttgggtt gattagtagc 300tttttgaatg gtaacttttc tacagaagta cgcggggctt ttttttttgc tgtaggcccg 360ggtggttgct gccgaaatgg gcangttcat gaaacctggg aaggtggtgc ttgtcctgct 420ggacgctact ncggacgcaa agctgtcatc gtgaaagaac attgatgatg gcaccttana 480cgccctacag ccatgctctg gtggctggaa ttgaccgcta cncccgaaag tgacagctgn 540catgggcaag aagaagatcg ccagagatca aagataaaan 580 465 578 DNA Homo sapiensmisc_feature (1)...(578) n = A,T,C or G 465 ggtacttttt tttttttttttttttttttt ttctacatca ctttanaata tttattgtat 60 tccttaatgc atttcttaacatgtatagca ctctttaatc aagaatataa agtcatctac 120 ttagaatcac attatcttaaagatgcatac tggaatgata agtttgaaga tgtaactatc 180 aacaattctt ttcaaaatcatatcaatata ttactctcat ggaacttgca cattctaaga 240 agggtcattt tttccccccagtaccaatat tacattattt gacagggata ataaaatgag 300 cagagactgg aaatcacagacaataacatt gctttctcaa ttaacagaaa ggattcataa 360 catattcctt aacggtagatgtgatttgta gagaatgtgg aaaagaacta ttgagaagtc 420 cacctgctgc ccaaactgaggcacattagg gtggttgtgg gangagttat atttgagggt 480 ccatttttcc ttagggtttaaaagcatgtc cnggttggng gtnatttgcc attaagtctn 540 ttttcaaata aaagaattaggggagaaagt ttggaaaa 578 466 546 DNA Homo sapiens misc_feature(1)...(546) n = A,T,C or G 466 accaatacca ccaattttgt agacatcctggagaggcagg cgcaagggct tgtcagttgg 60 acgagttggt ggtaggatgc agtccagagcctcaagcagc gtggttccac tggcattgcc 120 atccttacgg gtgactttcc atcccttgaaccaaggcatg ttagcacttg gctccagcat 180 gttgtcacca ttccaaccag aaattggcacaaatgctact gtgtcggggt tgtagccaat 240 tttcttaatg taagtgctga cttccttaacaatttcctca tatctcttct ggctgtaggg 300 tggctcagtg gaatccattt tgttaacaccgacaattagt tgtttcacac ccagtgtgta 360 agccagaang gcatgctctc gggtctgcccattcttggag ataccagctt caaattcacc 420 aacaccagca gcaacaatca ggacagnacagtcggnctga gatgtccctg taatcatgtt 480 ttgataaaag tctctgtgtc ctggggcatcaatgatagtc acatagtacc tcggccgcga 540 ncacgc 546 467 445 DNA Homo sapiensmisc_feature (1)...(445) n = A,T,C or G 467 acctaaaacc cgaagaaccttctgtaagaa gtgtggcaag catcagcctc acaaagtgac 60 acagtataag aagggcaaggattctttgta tgcccaggga aggaggcgct atgatcggaa 120 gcagagtggc tatggtgggcagacaaagcc aattttccgg aagaaggcta agaccacaaa 180 gaagattgtg ctaaggctggaatgtgttga gcctaactgc agatccaaga ggatgctggc 240 tattaagaga tgcaagcattttgaactggg aggagataag aagagaaagg gccaagtgat 300 ccagttctaa actttgggatatttttcttc aattttgaag agaaaatggt gaaccataga 360 aaagttaccc gagggaaaataaatacagtg atattccaaa aaaaaaaann nnnnnaaaaa 420 aaagtncttg gccgggaccccctaa 445 468 566 DNA Homo sapiens misc_feature (1)...(566) n = A,T,C orG 468 actgtgtggc gccttattct aggcacttgt tgggcagaat gtcacacctg ccgatgaaac60 tcctgcgtaa gaagatcgag aagcggaacc tcaaattgcg gcagcggaac ctaaagtttc 120agggggcctc aaatctgacc ctatcggaaa ctcaaaatgg agatgtatct gaagaaacaa 180tgggaagtag aaaggttaaa aaatcaaaac aaaagcccat gaatgtgggc ttatcagaaa 240ctcaaaatgg aggcatgtct caagaagcag tgggaaatat aaaagttaca aagtctcccc 300agaaatccac tgtattaagc aatggagaag cagcaatgca gtcttccaat tcagaaccaa 360aaaaaaaaaa nnaaaaaaag tacttttttt tntnnnnnnn ttttttttag gaatgggtgt 420tgaacttgac ctttcttaat gggggctggt tttaggctat atggngtaaa tttttctctt 480ttacaaggtt tttcctagng ncaaaaactg tcctttggac taccgtaaat tacaggggtt 540taaaggttnt ggggcaatta aanttn 566 469 586 DNA Homo sapiens misc_feature(1)...(586) n = A,T,C or G 469 acgcgggata ggtttggtcc tagcctttctattagctctt agtaagatta cacatgcaag 60 catccccgtt ccagtgagtt caccctctaaatcaccacga tcaaaaggga caagcatcaa 120 gcacgcagca atgcagctca aaacgcttagcctagccaca cccccacggg aaacagcagt 180 gattaacctt tagcaataaa cgaaagtttaactaagctat actaacccca gggttggtca 240 atttcgtgcc agccaccgcg gtcacacgattaacccaagt caatagaagc cggcgtaaag 300 agtgttttag atcaccccct ccccaataaagctaaaactc acctgagttg taaaaaactc 360 cagttgacac aaaatagact acgaaagtggctttaacata tctgaacaca caatagctaa 420 gacccaaact gggattagat accccactatgcttagccct aaacctnaca gttaaatcaa 480 caaaactgct cgccagacac tcgagccacagcttaaaact caaggacctg cgggcttcat 540 atccctctag angacctgtc tgtaatcgataaccccgatc aacctn 586 470 487 DNA Homo sapiens misc_feature (1)...(487)n = A,T,C or G 470 acggccaggg ctattggttg aatgagtagg ctgatggtttcgataataac tagtatgggg 60 ataaggggtg taggtgtgcc ttgtggtaag aagtgggctagggcattttt aatcttagag 120 cgaaagccta taatcactgc gcccgctcat aaggggatggccatggctag gtttatagat 180 agttgggtgg ttggtgtaaa tgagtgaggc aggagtccgaggaggttagt tgtggcaata 240 aaaatgatta aggatactag tataagagat caggttcgtcctttagtgtt gtgtatggtt 300 atcatttgtt ttgaggttag tttgattagt cattgttgggtggtgattaa tcngttgntg 360 atgaaatatt tggaggtggg gatcaatana gggggaaatagaatgatcag tacctcgccc 420 gcgaccacgc taagggccaa tccacacact ggcggncgtactaatggatc ccaactcgga 480 ccagctt 487 471 488 DNA Homo sapiensmisc_feature (1)...(488) n = A,T,C or G 471 actgcggcgg gtaggcctaggattgtgggg gcaatgaatg aagcgaacag attttcgttc 60 attttggttc tcagggtttgttataatttt ttatttttat gggctttggt gagggaggta 120 ggtggtagtt tgtgtttaatatttttagtt gggtgatgag gaatagtgta aggagtatgg 180 gggtaattat ggtgggccatacggtagtat ttagttgggg cattcccgcg tacctatttg 240 tatttttggt agagacagggttttgccatg ttggccagga tggtcttgaa ctactgacct 300 caggtgatcc tcacgcctttatctcccaaa gtgctgcgat tacaggcatg aggcaccact 360 cctggccaca ttcttatatttaaaaaaaaa gcacaactct attgtctact ggtgttcttt 420 tacctgaagt tcaaactctagctcttcaaa aaaaaaaaaa aaaaaaagta cctnggccgc 480 naccacnc 488 472 586 DNAHomo sapiens misc_feature (1)...(586) n = A,T,C or G 472 ggtacttgatgccctccaag caattaaaac caagggcaaa cgagccccat tcacaaattt 60 tgacccctctactctccttc cttcatccct ggatttctgg acctaccctg gctctctgac 120 tcatcctcctctttatgaga gtgtaacttg gatcatctgt aaggagagca tcagtgtcag 180 ctcagagcagctggcacaat tcagcagcct tctatcaaat gttgaaggtg ataacgctgt 240 ccccatgcagcacaacaacc gcccaaccca acctctgaag ggcagaacag tgagagcttc 300 attttgatgattctgagaag aaacttgtcc ttcctcaaga acacagccct gcttctgaca 360 taatccagtaaaataataat ttttaagaaa taaatttatt tcaatattag caaagacagc 420 atgccttcaaatcaatctgt aaaactaaga aacttaaatt ttagttctta ctgcttaatc 480 aaataataattagtaagcta gcaaatagta atctgtaagc ataagcttat gcttaaatca 540 gtttagtttgaggaatcttt aaaattacca ctaantgatt gnatgg 586 473 575 DNA Homo sapiensmisc_feature (1)...(575) n = A,T,C or G 473 ggtacaaagg ggaaagggtgcatgccaact atcgaattat aggatatgta aaaaatataa 60 gtcaagaaaa tgccccagggcccgcacaca acggtcgaga gacaatatac cccaatggaa 120 ccctgctgat ccagaacgtcacccacaatg acgcaggaat ctatacccta cacgttataa 180 aagaaaatct tgtgaatgaagaagtaacca gacaattcta cgtattctcg gagccaccca 240 agccctccat caccagcaacaacttcaatc cggtggagaa caaagatatt gtggttttaa 300 cctgtcaacc tgagactcagaacacaacct acctgtggtg ggtaaacaat cagagcctcc 360 tggtcagtcc caggctgctgctctccactg acaacaggac cctcgttcta ctcacgccca 420 aagaatgaca taggaccctatgaatgtgaa atacagaacc cagtgggtgc cacccgcant 480 gcccantcac cctgaatgtccgtatgagtc aatcctgccg gcggccgttc naanggcgaa 540 ttccacacac tggcggccgtctaatggatc cactc 575 474 515 DNA Homo sapiens misc_feature (1)...(515) n= A,T,C or G 474 ggtacgtggg ggactcaact gaaatcatgg cgtttgacag cacttggaaggtagaccgga 60 gtgaaaacta tgacaagttc atggaaaaaa tgggtgttaa tatagtgaaaaggaagcttg 120 cagctcatga caatttgaag ctgacaatta cacaagaagg aaataaattcacagtcaaag 180 aatcaagcgc ttttcgaaac attgaagttg tttttgaact tggtgtcacctttaattaca 240 acctagcaga cggaactgaa ctcaggggga cctggagcct tgagggaaataaacttattg 300 gaaaattcaa acggacagac aatggaaacg aactgaatac tgtccgagaaattataggtg 360 atgaactagt ccagacttat gtgtatgaag gagtagaagc caaaaggatctttaaaaagg 420 attgaccatt attcttggcg cacagtccaa aatncaaatt ggccagaagatctatattgn 480 acctgcccgg gcggccgttc gaaaggccaa ttcca 515 475 580 DNAHomo sapiens misc_feature (1)...(580) n = A,T,C or G 475 acaaagatctgacatgtcac ccagggaccc atttcaccca ctgctctgtt tggccgccag 60 tcttttgtctctctcttcag caatggtgag gcggataccc tttcctcggg gaagagaaat 120 ccatggtttgttgcccttgc caataacaaa aatgttggaa agtcgagtgg caaagctgtt 180 gccattggcatctttcacgt gaaccacgtc aaaagatcca gggtgcctct ctctgttggt 240 gatcacaccaattcttccta ggttagcacc tccagtcacc atacacaggt taccagtgtc 300 gaacttgatgaaatcagtaa tcttgccagt ctctaaatca atctgaatgg tatcattcac 360 cttgatgaggggatcggggt agcggatggt gcgggcatca tgagtcacca gatgagggat 420 tccttttgtgccccaaagat ctttctnact ttgacaactt gaccttggnc gcgaccaccc 480 taaggcgaattcacccactg gcggccgtct aatggatccn nctcggncca acctggntat 540 atggcntaantnntccnggn naaatntntc cccncaatcc 580 476 593 DNA Homo sapiensmisc_feature (1)...(593) n = A,T,C or G 476 ggtactatgt gggacagtattttgcaaata caagaagagc tcagggcagc tgtggagctg 60 gatggtctgc ctggcaggcctctgtgcagt ctgcctgctc atcctgtccc ctttttgggg 120 cttgatcctc ttctcggtgtcatgcttcct catgtatact tacttatctg gccaagaatt 180 gttacctgtg gatcagaaggcagtcctggt gacaggtgtg attgcgggct tggccatgct 240 ttgtgcaagt atctggatgagctgggcttc acggtatttg ccggagtttt gaatgaaaat 300 ggcccaggag ctgaggaattgcgaagaacc tgctctccgc gcctctcggt gctccaaatg 360 gacatcacga accagtgcagataaaagatg cttacagcaa ggttgcaaca atgctgcagg 420 acaaaagact gtgggctgtgatcaacaatg ctngggtgct tggcttttcc actgatgggg 480 agcttnttnt tatgatgactacnaacaatc ntggccgnga acttttttga actgngaggg 540 acaaaacgtt tttccttttttaaaaaancc aagggnggtg gnnaattncn nnt 593 477 595 DNA Homo sapiensmisc_feature (1)...(595) n = A,T,C or G 477 actacaaggt ttagcatttgctctgctggt cgacattccc ccagtctatg ggttgtatgc 60 atcctttttc ccagccataatctacctttt cttcggcact tccagacaca tatccgtggg 120 tccgtttccg attctgagtatgatggtggg actagcagtt tcaggagcag tttcaaaagc 180 agtcccagat cgcaatgcaactactttggg attgcctaac aactcgaata attcttcact 240 actggatgac gagagggtgagggtggcggc ggcggcatca gtcacagtgc tttctggaat 300 catccagttg gcttttgggattctgcggat tggatttgta gtgatatacc tgtctgagtc 360 cctcatcagt ggcttcactactgctgctgc tgttcatgtt tttggnttcc caactcaaat 420 tcatttttca agtgacagtcccgtcacaca ctgatncagt ttnaatttta aaagtacctc 480 ggccgcganc accctaaggcgaatttnaac ccactngcgg ccgttctant ggatccaact 540 ngnnncaaac ttngngaatangggcataac ngntcctggg gaaatnnttc ccnct 595 478 420 DNA Homo sapiensmisc_feature (1)...(420) n = A,T,C or G 478 ggtacacagt atgtataacaatgcatacta tggtgtggag ttaattccaa ttaccatatt 60 ttatatttat tggtcacaacagcatacatt ttatgctcca aaatacatgg atctgacaaa 120 atggttacat ttaatgttcttttaaagaaa gatgaactaa atttaagaag aattggtttt 180 tcctaatatc tcattttcaaattactgata caaatttgcc agagaaacaa ttacatgttt 240 tacctaacat caaataatctccagtttcta agacagatgc atttcttgtt caatttccaa 300 aagtaaataa aggctttctaactgaaaaca tttgcatccc tagctctcta aagtaattaa 360 aaagaaaatt acaaaaaatgacctctaagc ttctgaacag cccacttant tacataaagt 420 479 602 DNA Homo sapiensmisc_feature (1)...(602) n = A,T,C or G 479 ggtacctagt cagatggtagacgagctgtc tgctgccgca ggagcacctc tatacaggac 60 ttagaagtag tatgttattcctggttaagc aggcattgct ttgccctgga gcagctattt 120 taagccatct cagattctgtctaaaggggt tttttgggaa gacgttttct ttatcgccct 180 gagaagatct accccagggagaatctgaga catcttgcct acttttcttt attagctttc 240 tcctcatcca tttcttttatacctttcctt tttggggagt tgttatgcca tgatttttgg 300 tatttatgta aaaggattattactaattct atttctctat gtttattcta gttaaggaaa 360 tgttgagggc aagccaccaaattacctang ctgaggttag agagattggc cagcaaaaac 420 tgtgggaaga tgaactttgtcattatgatt tcattatcac atgattatag aaggctgtct 480 taatgcaaaa aacatacttacatttnanac atattccaan gggatctcnc attttgtaaa 540 aagttgacta ttactggagtaaaccctgtt ttccctaant ttaacttttt ttgggaaatt 600 at 602 480 600 DNA Homosapiens misc_feature (1)...(600) n = A,T,C or G 480 ggtacttttttttttttttt tttttttttc ggtttgaggg ggaatgctgg anattgtaat 60 gggtatgganacatgtcata taagtaatgc tagggtgagt ggtaggaagt tttttcatag 120 gaggtgtatgagttggtcgt agcggaatcg ggggtatgct gttcgaattc ataaaaacag 180 ggaggttanaagtagggtct tggtgacaaa atatgttgtg taaagttcag ggganagtgc 240 gtcatatgttgttcctagga aaattgtagt ggtgagggtg tttattataa taatgtttgt 300 gtattcggctatgaaaaata gggcgaaggg gcctgcggcg tattccatgt tgaagcctga 360 gactagttcggactcccctt cggcaaggtc caaaggggtt ccggttggtc tcttctagtg 420 tggagataaatcatattatg gccnagggtc atgatggcag gagtaatcaa aggggtcntt 480 tgttttgaaaaagggnggan aggttaaagg ancccctttt tataatggtg atantaaaaa 540 gatgcttgggggactcnttt aaaatgttgg ctcttcttcc angcncccac aggcgtattt 600 481 594 DNAHomo sapiens misc_feature (1)...(594) n = A,T,C or G 481 cgaggtacggccagggctat tggttgaatg agtaggctga tggtttcgat aataactagt 60 atggggataaggggtgtagg tgtgccttgt ggtaagaagt gggctagggc atttttaatc 120 ttagagcgaaagcctataat cactgcgccc gctcataagg ggatggccat ggctaggttt 180 atagatagttgggtggttgg tgtaaatgag tgaggcagga gtccgaggag gttagttgtg 240 gcaataaaaatgattaagga tactagtata agagatcagg ttcgtccttt agtgttgngt 300 atggttatcatttgttttga ggttagtttg attagtcatt gttgggtggt gattantccg 360 ttgttgatgagatatttgga ggtggggatc aatagagggg gaaatagaat gatcagtacc 420 tgcccnggcggncgctcgaa anggcgaatt ccaccacact ggcgggcgnt ctaatggatn 480 cgacccngtcccaacttgcg taatcatggc atacttgttn ctggtgaaat ggtatccctc 540 acaattcccacacatacaac ccgaacctaa atgtaaanct gggggcctat natn 594 482 600 DNA Homosapiens misc_feature (1)...(600) n = A,T,C or G 482 accatgaaatacatatattt cataaggttc agttacaaaa tggattgttt caaatggcaa 60 tttcttacactaacctgatt atgaaaaaaa gaagtctgta tcatctgctt ccaagtctgt 120 tatgtccaaatatattttaa ttatgcattt attttgctac ttttataaat attagagatt 180 tcaccttaaattatttttgt aactagttct agaacatgtt ttccaattat tatttttcta 240 atggagacatataattgacc tatgtttatg catatatgtt ctctacacag tgaaactttt 300 tttaaaaagaatagtaaaga aaatgcggaa gctctggctc tccaaggcaa agtcaaaaaa 360 aaaaaaaaagcgggggggaa tgcgaggaac attttattac acctnctgat tttcctcctt 420 gagntttattttctcccctt ggntatttgt taatgctaga aactgnattc ctaanaaagc 480 atacctctttcaggngagcn tgataattgg gaanaatttt gttcctttag tntgaacatt 540 ttattaagaagngattccta ataaaganac aangggctnt ttaattnttt gggggnngga 600 483 605 DNAHomo sapiens misc_feature (1)...(605) n = A,T,C or G 483 acagaacatcgtcagcacta gcacagttta cagaacctca cagacccaaa ggaacatcaa 60 taggcaaagcgactacagga ggcgtgtgtc cgcgtgggcg aggtaaagag ggtcagtatt 120 ggtcaagtgacagtgtcggt aatctggcaa gacagtgatg ttaagaaggt tcatagttta 180 agaattatctaaaatatttt aaaaactata aagctgcaac acatgatttt tacacctagt 240 tactagaaaactaaggaaag cacttattag ctctgaataa agtaacatgg aaagcacttt 300 tactaatcgacaaaaaaacc ttctaatgca ttatcagaaa gattttataa tacaaggagg 360 catattgctcagtcagaagg ggttctataa gaaaagcact tactaagtta gcgactaaca 420 gaacaaccngtttaaagatg aattaaatgc cccatttggg gangcatggc aggtgttaag 480 anaaangaaaagcntaagaa aacatttnct ggttatanca aacctttntt tnttatctac 540 tgnatttgacaaaaattaac cntttaaagt tttacccngg cacttnnttc nttgtcctcg 600 gcccg 605 484591 DNA Homo sapiens misc_feature (1)...(591) n = A,T,C or G 484ggtacgcggg tggggagacc ctggggtagc agccactgac ctcacacctg gaggaagctg 60tgtgaccgat tcatgagctt atgcctgaag acagagcaag cactccccgc accacgacga 120tgacgttcac ttgttttgtg tttttcgatc tcttcaacgc cttgacctgc cgctctcaga 180ccaagctgat atttgagatc ggctttctca ggaaccacat gttcctctac tccgtcctgg 240ggtccatcct ggggcagctg gcggtcattt acatcccccc gctgcagagg gtcttccaga 300cggagaacct gggagcgctt gatttgctgt ttttaactgg attggcctca tccgtcttca 360ttttgtcaga gctcctcaaa ctatgtgaaa aatactgttg cagccccaaa gagagtccag 420atgcaccctg aaagatgtgt agtggaccgc acttccgcgg naccttccta atnatttcaa 480ctgggtgnga ctgtggccct gccctgtttc ttcttagggg agactttang anggcgagcn 540tcataccgga tagttttctt taggaaactn aggaaccttg gctcaggacc a 591 485 605 DNAHomo sapiens misc_feature (1)...(605) n = A,T,C or G 485 ggtacgcggggatataaagg gagagagcaa gcagcgagtc ttgaagctct gttnggtgct 60 tnggatccatttccatcggn ccttacagcc gctcgtcaga ctccancagc caanatggtg 120 aancagatcgagagcaagac tgcttttcan gaagccttgg acgctgcang tgataaactt 180 gtagnagttgacttctcagc cacgtggtgt gggccttgca aaatgatcaa gcctttcttt 240 cattccctctctgaaaagta ttccaacgtg atattccttg aagtagatgt ggatgactgt 300 caggatgttgcttcagagtg tgaagtcaaa tgcatgccaa cattccagtt ttttaagaag 360 ggacaaaaggtgggtgaatt ttctggagcc aataaggaaa agctttgnag ccnccattaa 420 tgaatgagtctaatcatgtt ttctgaaaac ataacccagc catttggcta tttaaaactt 480 gnaantttttnagntaccna aatttaaagt ctgaagacat aacccggtgc catttgcgtg 540 acaatnaaaaattatgccaa cactttttna anaanganan nnntttcctn gggaaatngt 600 anccc 605 486319 DNA Homo sapiens 486 ggtaccagtt gtagccataa agattctggg actcattatggactactaga aggacctcct 60 tcccttctgc gacattgaac ggcacgacat caatattggtctgggcactg tttggcaggt 120 tccagaaggt taaaagcgag gctgtgagca ggagtccctgccagggaatg cacactctgt 180 atggacaggc tgaaggggac cccatggtct ctgctgcctgcttgtcctct gtggagaaga 240 gcttgggctc caggaactct cttgtcaggg ctgctgtgactgtcagctct gctgtccttc 300 ctacctctgt gtcccgcgt 319 487 586 DNA Homosapiens misc_feature (1)...(586) n = A,T,C or G 487 acgcgggagctgagtgtccc gcggggcccg aagcgtttac tttgaaaaaa ttagagtgtt 60 caaagcaggcccgagccgcc tggataccgc agctaggaat aatggaatag gaccgcggtt 120 ctattttgttggttttcgga actgaggcca tgattaagag ggacggccgg gggcattcgt 180 attgcgccgctagaggttaa attcttggac cggcgcaaga cggaccanag cgaaagcatt 240 tgccaagaatgttttcatta atcaagaacg aaagtcggag gttcgaagac gatcagatac 300 cgtcgtagttccgaccataa acgatgccga ccggcgatgc ggcggcgtta ttccatgacc 360 cgccgggcagctttcnggaa accaaagtct ttgggttncc gggggagtat ngttcnaaaa 420 aaaaaaaaaaaaaaaaaagt cctnggccgg gancccctta nggngaaatt cagccactgg 480 nggcgttctnatggatncna gctcggncca acntggcgta atatggcata cttgttcctg 540 gngnaaatgtttccctccaa attccccaaa tacgggcgga gcttaa 586 488 487 DNA Homo sapiensmisc_feature (1)...(487) n = A,T,C or G 488 acagctggtt ggacctattcatgcatcttc accagcagct ggagcatctc cacccttggt 60 atttctggtg taaattacttgagctctgtg ctttgaaacc agtttgataa gtcctttact 120 aaggagctcc tgaagggctgccctggccag ggagcctcga atcttcagtc tctcagagac 180 cacagctggg gttataagtttatagttggg aacttcctta cagagtttat cataggtagc 240 tttgtcaaac aagactaagttattgagctt gtcccgaact ttgcctttgg accacttctt 300 ctttttggcc ttgcccccggatttgttcac tgggtctttg tctttcttgg ccgactttcc 360 agcgtccttc ttcttcttgtcgtccttaag cggcattgcg aanctcggag aataagcaac 420 aaacaccgca cctcgtcnaagatgtcggac aaaaaaaggc cccgcgtacc ttnggccgcg 480 ancacnc 487 489 589 DNAHomo sapiens misc_feature (1)...(589) n = A,T,C or G 489 acgcggggtctctcctcagg cagcagcaac gcggaggaaa cgggagtgaa cggagagcgt 60 agtgaccatcatgagcctcc tcaacaagcc caagagtgag atgaccccag aggagctgca 120 gaagcgagaggaggaggaat ttaacaccgg tccactctct gtgctcacac agtcagtcaa 180 gaacaatacccaagtgctca tcaactgccg caacaataag aaactcctgg gccgcgtgaa 240 ggccttcgataggcactgca acatggtgct ggagaacgtg aaggagatgt ggactgaggt 300 acaaagattaaattaagaca cggtaaattg actaaatatt tggtttttat ataaataaag 360 gtcataaccacaccgttgac atgtaatact gttataatac aacagttaaa ctttgtgagt 420 ctcaacagaagtcatctgta gttnaacagg aaacaaaagt tgaaaaaaaa catgttnaaa 480 caaaactctgggactaacag gtcgggattg taagtacaac caacatattc ctcacttctg 540 ggtntttcaagtttacagta cttggccgga cccccttang ggnattcac 589 490 591 DNA Homo sapiensmisc_feature (1)...(591) n = A,T,C or G 490 ggtaccggga tagtttttgcagggttttat tttataaaat ccaagcgcgc tgttgattgt 60 gttttccttg ttttcagccccccgactcca gcccgcagca catttccgct gtccgtcagt 120 aattgtgtcc tctctttatgcttgcttggg gaatgttgtt ttctgactag gctgatcatt 180 atctaaagaa tctaattctgttgattttta aaacttttag gaccataaac gttgtgttca 240 tatatggaca tggaaatatttatataattt tatagaaaat aaccttttag atggtcaaag 300 tgtaaggagt tttttttgtcagataatcat ttctacttca aaaacatttc atgcaatatt 360 agaataaagt tcctgtcattcctctnnnan aaaaannnnn nnnnnnanna nnnnnnnnnn 420 nggaanannn nnnnnnnnnnaaaaaagtac ctgcccnggc ggccgttcaa aaggcgaatt 480 ccacccactg gcggccgttctaatggatcc anctcggacc aacctggnga aacatggcat 540 acctgttcct ggngaaatggtntcccttac aattcccaca aataaaaccg g 591 491 583 DNA Homo sapiensmisc_feature (1)...(583) n = A,T,C or G 491 ggtacatata aatccttttggtgtcacttg tagcaagcct tgcttctgca gttttcggat 60 tttcctcaaa gctttgttgcgcttgcgtag aattcgaagt ggactaaagc caacagcatc 120 gataagtttc cgcctaaagaaaccaatgtt tgcaaagtag ataggagatg gacatctgaa 180 aattttcact ccttctggctcatacatatc ataataatct tttttattct tatagatgtt 240 ggttcttcca atattagccagcgtgctgca ttttggaaat tgggtcctga acacgatggt 300 tagcagttga aatgccacactagctgccag gcctaacccg agtcccagga caatggtgaa 360 agatgaaagg catgaacccaaataaacaat catatttggn cnttccccca atctgctatt 420 ttaaccaact gcatcaacattcctttaagt tccaatgcta aactggcang acnggcnttt 480 gtagaagngc cangaaaaatcagngcttga cgacaatcac accatgatgn nccataancc 540 acaatctggg nttggctcnnggcctctgaa cnnngactgg nag 583 492 597 DNA Homo sapiens misc_feature(1)...(597) n = A,T,C or G 492 acgcgggggg tggcacggag gaaccaggagcgtgccctgc gcaccgtctg gagctccgga 60 gatgacaagg agcagctggt gaagaacacatatgtcctgt gaccgccctg tcgccaagag 120 gactggggaa gggaggggag actatgtgtgagcttttttt aaatagaggg attgactcgg 180 atttgagtga tcattagggc tgaggtctgtttctctggga ggtaggacgg ctgcttcctg 240 gtctggcang gatgggtttg ctttggaaatcctctangag gctcctcctc gcatggcctg 300 cagnctggca acaaccccga gttgtttcctcgctgatcga tttctttcct ncaggtagag 360 ttttctttgc ttatgttgaa ttccattgcctttttctcat cacaaaaaat gatgttggga 420 atcgnntctt ttgtttggct gaattatgggntttttaant ataaaccaaa nttttttatt 480 aacattctta aanaagggaa agtnnaatgtncnttggncc cnaccncgct aanggcnaat 540 ttcancccnt ggnggccgtn nttnnggatccnnncnngnn ccaannntgg nntantn 597 493 591 DNA Homo sapiens misc_feature(1)...(591) n = A,T,C or G 493 acggatgcta cttgtccaat gatggtaaaagggtagctta ctggttgtcc tccgattcag 60 gttagaatga ggaggtctgc ggctaggagtcaataaagtg attggcttag tgggcgaaat 120 attatgcttt gttgtttgga tatatggaggatggggatta ttgctaggat gaggatggat 180 agtaataggg caaggacgcc tcctagtttgttagggacgg atcggagaat tgtgtaggcg 240 aataggaaat atcattcggg cttgatgtggggaggggtgt ttaaggggtt ggctagggta 300 taattgtctg ggtcgcctag gaggtctggtgagaatagtg ttaatgtcat taaggagaga 360 aggaagagaa gtaagccgag ggcgtctttgattgtgtagt aagggtggaa ggtgatttta 420 tcggaatggg aggtgattcc taagggggtggttgatcccg tttcctgcca agaataagaa 480 gtggaatgct gctagggctg cattaatgaaggccaagatg aaatgaaagg taaanaatcn 540 ngtgangggg gactgctact gatancctctcaaatcatga ataggntgtc c 591 494 374 DNA Homo sapiens misc_feature(1)...(374) n = A,T,C or G 494 ggtacttttt tttttttttt ttttttttttttttttagnt catgtctttt attaactcat 60 acagttactt gtcttctggt ttgttgaaacagtaagtcan acaacatttg ccacaataat 120 gtctgtcaaa gtgacttgcc ataaacaccccagcaccaca ttcatcanaa gggcactctc 180 gacgaaggcg actaattttg ccattctcatccaccttata atatttcagg acagccagct 240 taaccttctt tctcttgtgc ttattcttcttgggagnggt gtaagacttc ttcttccttt 300 tcttagcacc accacgaagt ctcaacacaagatgaagagt agactccttt tgaatattgt 360 aagtcagaca aagt 374 495 597 DNAHomo sapiens misc_feature (1)...(597) n = A,T,C or G 495 actgggagaaggtgctgacg ccgacgaagt ggtggatggg cttcccgctg caggtgaacc 60 tcctggtgccatcctgcagg gtcccccgag gattgcctag atcatttttc aagcagtagt 120 tgctttctgggtttttacaa attctgcatt ttccacactg aggagtaaag agcgggatga 180 ctttatcacctggtttgact gtagtcaccc cttctccaac actttccacg atgccggctg 240 cctcatggcctaaaatcaca ggaagggggg tcaccaggtt gccactaacc acatgctcat 300 ctgaacgacagattcctgca gccaccatct taatgcgaac ttcatgagcc ttaggaggtg 360 caacctctacctcctcaatg gaaaagggtt tctttaactc ccatagcaca actgctttgc 420 atttgattacctgtaaactc agctacttgt gaaggctgag gcanganaat actttgaacc 480 ccggaaggcaaaggttgcaa tgagccnana acaccattgn acttccanct gggcaatana 540 aaaaaactcatttttcctgc tggctcaaat gatctgcttc ttgcaaacaa gagntgn 597 496 604 DNA Homosapiens misc_feature (1)...(604) n = A,T,C or G 496 ggacgcgggtgctgactgca tagctctttt tcttgagagg ctctccattt tgattcagaa 60 agttagcatatttattacca atgaatttga aaccagggct tttttttttt tttgggtgat 120 gtaaaaccaactccctgcca ccaaaataat taaaatagtc acatttatct ttattaggta 180 atcacttcttaattatatgt tcatactcta agtatcaaaa tcttccaatt atcatgctca 240 cctgaaagaggtatgctctc ttaggaatac agtttctagc attaaacaaa taaacaaggg 300 gagaaaataaaactcaagga gtgaaaatca ggaggtgtaa taaaatgttc ctcgcattcc 360 cccccgcttttttttttttt ttgactttgc cttggaaagc cagagcttcc cgcattttct 420 ttactattctttttaaaaaa agtttcactg ngtaaaagaa catatttgcc taaacatang 480 tcaattatatgtctccatta naaaaaaata attggnaaac attgtctana actagttcca 540 aaataattaagggggaaatc tntaatnttt ttaaagtgcc naaanaatgc ctaanttaaa 600 antt 604 497587 DNA Homo sapiens misc_feature (1)...(587) n = A,T,C or G 497acattaatga aatgtttcca aagaaatact gaacaatata tactctagtt tgctgaggtt 60ccagctcgag ttcaaaccta attcttgtgc aataaaaatc agcatggatc ttagatgatc 120tagaatacac tgtgttttga aatccacagc tggtttcatt tttaaccatt atgaaaaacc 180agtacttttt tttttttttt tttttttttc nctnggacca taaattttta ttggcaggtc 240aggaaaaaag ccgggggtaa gggtcccttc cttcccatcc ctctacccan aanacaccct 300ccaaaggaca gcagaagccc cagagcctgc tgcctcagag gaccttggag gcagacaaat 360tgttgtagng atcttcctgt ccctcaanca ggctgcggta ggtggnaatc tnctgctcca 420gccgcgactt gatgtccatg aaccgctggt cctcggccgc gacaccctta nggcgaattn 480caccnactgg gnggcgttct agtggatccg actcggacca acctngcgna atcatggcan 540actggttnct gnnggaaatg gtttccctnc aattccccaa cataccn 587 498 354 DNA Homosapiens 498 acgcgggcaa taaagctaaa actcacctga gttgtaaaaa actccagttgacacaaaata 60 gactacgaaa gtggctttaa catatctgaa cacacaatag ctaagacccaaactgggatt 120 agatacccca ctatgcttag ccctaaacct caacagttaa atcaacaaaactgctcgcca 180 gaacactacg agccacagct taaaactcaa aggacctggc ggtgcttcatatccctctag 240 aggagcctgt tctgtaatcg ataaaccccg atcaacctca ccacctcttgctcagcctat 300 ataccgccat cttcagcaaa ccctgatgaa ggctacaaag taagcgcaagtacc 354 499 632 DNA Homo sapiens misc_feature (1)...(632) n = A,T,C orG 499 nccgaggtac caactgcact cgttttggca ttgcagctaa atatcagttg gatcccactg60 cttccatttc tgcaaaagtc aacaactcta gcttaattgg agtaggctat actcagactc 120tgaggcctgg tgtgaagctt acactctctg ctctggtaga tgggaagagc attaatgctg 180gaggccacaa ggntgggctc gccctggagt tggaggctta atccanctga aaagaaacct 240ttgggaatgg atatcaaaag aattggcctt aatatatttc cattgngacc agcagcaggc 300tttttttccc ccagaagatg atcaaaacaa aaggatgatc tcaacaagaa ctgtatttta 360aagtatttaa ganagtcttt ggtaactngg ttctaagtng gtatctaatt acccaatgct 420gcagtcctgc agtccctatt cattanttaa atgtatttaa ctggtaaatg ccctncccnc 480cataatgaaa taganccttt ttgaaaaccc aaaaaaaaaa aaaaaaaaaa aaaaaagtcc 540ctgcccggcc ggccctcaaa nggngaattc cannccctgg gggccgtact aanggatccn 600cccggnccaa cttggggaat atgggntant gn 632 500 619 DNA Homo sapiensmisc_feature (1)...(619) n = A,T,C or G 500 tccagcggnc cgccgggcnggtcatctata aaaggaaaag tgatggcatc tatatcataa 60 atctcaagan gacctgggagaagcttctgc tggcagctcg tgcaattgtt gccattgaaa 120 accctgctga tgtcagtgttatatcctcca ngaatactgg ccaaanggct gtgctgaant 180 ttgctgctgc actggaaccactccaattgc tggccgcttc actcctggaa ccttcactaa 240 ccagatcagg caaccttccgggaccacggn ttnttgtggt tactgacccc aaggctgacc 300 accaacctnt cacggaggcattttatgtta acctacctac cattgcgctg tgtaacacaa 360 gattcttctc tgcctatgtggacattggca ttccatgcaa caaccaaggg gagctcactc 420 aatgggtttg atgtggtggatctgctcggg naagtctgcg catgcctggc accatttccg 480 tgaacaccat ggagggatgcctgattttac cttggccgga cacnctangg cgaattcacc 540 acttggngcc gtatantggatccactcgga ccaacttggg naaaatggca naatnttccg 600 gggaaatgat ccctccaan 619501 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,C or G 501accacactga gatagtgttt gccaggacct cccctcagca gaagctcatc attgtggaag 60gctgccaaag acagggtgct atcgtggctg tgactggtga cggtgtgaat gactctccag 120ctttgaagaa agcaaacatt ggggttgcta tggggattgc tggctcagat gtgtccaagc 180aagctgctga catgattctt ctggatgaca actttgcctc aattgtgact ggagtagagg 240aaggtcgtct gatctttgat aacttgaaaa aatccattgc ttatacctta accagtaaca 300ttccccgaga tcaccccgtt cctgatattt attattgcaa acattccact accactgggg 360actgtcacca tcctctgcat tgacttgggc actgacatgg gtnctgccat ctcctggctt 420atgagcaggc tggagggcat catgaanaaa cagcccaaaa tccaaacaga caacttgtga 480atgancnggt gatcacatgg ctatggcaga atggatgatc nagncctggg aggttcttac 540ttacttggaa tctgntgaaa cggttcttcc aatacctntt ggcctccatg gntggaanac 600cctga 605 502 627 DNA Homo sapiens misc_feature (1)...(627) n = A,T,C orG 502 acatcttgct ggaaaatgct gcccagggct ctggagacgg tggctgcccg ggctcccttc60 actgtccagg tcctgaaaga ctcttgttca tgaactgtct cttcacaaag caagtccacc 120acttgctggg tttatcattc tgagggtcga aaactttctc acaaagtctc agtccagtct 180cttgccttag ctgttgtaaa taggctctca tcacttcatc ttctgtttgt ttgcaggttt 240ggcataaatt gcgttaagtg gaaaaccagg ctctccagga atgggaaaat taagtgattc 300ccagcgtata catttctttc tcaccttggc ttttggaatt gcacttttgc agtttcttca 360nacattcaga aatgtagaga gttatatata tcaangncct atcaacttca ttcttaattt 420cataagtttt gaaaaaaaca ttggcccttg aagtaataaa tngntttatt cccaaaatct 480ggatcntttg gcnctctngg ggcangnccc ttgaaatgac ttttgatagg gaacaangcc 540ctggtttcca nnagnttggg ttcnggaccn taaaaaaaaa gggaanccgg nttttggngg 600gcccggttta acccaagggc cggancn 627 503 629 DNA Homo sapiens misc_feature(1)...(629) n = A,T,C or G 503 ggtacattag tagagctctc caatcacaggcagacgccag tgtcctatga ccagggggca 60 aatatggcca aacagattgg agcagctacttatatcgaat gctcagcttt acagtcggaa 120 aatagcgtca gagacatttt tcacgttgccaccttggcat gtgtaaataa gacaaataaa 180 aacgttaagc ggaacaaatc acagagagccacaaagcgga tttcacacat gcctagcaga 240 ccagaactct cggcagttgc tacggacttacgaaaggaca aagcgaagag ctgcactgtg 300 atgtgaatct ttcattatct ttaatgaagacaaaggaatc tagtgtaaaa aacaacagca 360 aacaaaaagg tgaagtctaa atgaagtgcacagccaaagt catgtatcca gaggcttang 420 aggcgtttga gangatactc atctttttggaatnctgcct taggttcggc atgtanacca 480 agtgatgaga agtgaatcca tggaagagttttaatgtgac ttggaaaata tgccaaaaaa 540 tgagagatcc aataacttna ggaaaataagggggatccaa tncctncccg gcggccctta 600 ggggaattca aacactnggg gcggtatan 629504 462 DNA Homo sapiens misc_feature (1)...(462) n = A,T,C or G 504acgcgggagc tgagtgtccc gcggggcccg aagcgtttac tttgaaaaaa ttagagtgtt 60caaagcaggc ccgagccgcc tggataccgc agctaggaat aatggaatag gaccgcggtt 120ctattttgtt ggttttcgga actgaggcca tgattaagag ggacggccgg gggcattcgt 180attgcgccgc tagaggttaa attcttggac cggcgcaaga cggaccagag cgaaagcatt 240tgccaagaat gttttcatta atcaagaacg aaagtcggag gttcgaagac gatcagatac 300cgtcgtagtt ccgaccataa acgatgcccg accggcgatg cggcggcgtt attccatgac 360ccgncgggca gcttccggga aaccaaagtc tttgggttcc ngggggagta tnggtgcaaa 420aaaaaaaaaa aaaaaaaaaa gtcctnggnc gcgaccccct aa 462 505 628 DNA Homosapiens misc_feature (1)...(628) n = A,T,C or G 505 actttttttttttttttttt tttgggggag gttatatggg tttaatagtt tttttaattt 60 atttagggggaatgatggtt gtctttggat atactacagc gatggctatt gaggagtatc 120 ctgaggcatgggggtcaggg gttgaggtct tggtgagtgt tttagtgggg ttagcgatgg 180 aggtaggattggtgctgtgg gtgaaagant atgatggggt ggtggttgtg gtaaacttta 240 atagtgtaggaagctgaata atttatgaag gagaggggtc agggttgatt cgggaggatc 300 ctattggtgcgggggctttg tatgattatg ggcgttgatt agtantaatt actggttgaa 360 cattgtttgttggtgtatat attgnaattg agattgctcg ggggaatang ttatgtgatt 420 aggaatagggttangatgag tgggaagaaa aaaagaaagg aantaaaagt ttaattattc 480 cctttttgggttgaagngat natggaaggg gaaaatttgg gccttgaaat tgtttaagta 540 atacttttctaataaggtaa gtctagaaga atagggcngg ttttggtctt aaaaaggcta 600 aaaggggattggcggggtgg atccnccc 628 506 612 DNA Homo sapiens misc_feature(1)...(612) n = A,T,C or G 506 acggtagaac tgctattatt catcctatgtgggtaattga ggagtatgct aagattttgc 60 cgtagctggg tttggtttaa tccacctcaactgcctgcta tgatggataa gattgagaga 120 gtggggagaa ggcttacgtt cagtgagggagagatttggt atatgattga gatgggggct 180 agtttttgtc atgtgagaag aagcaggccggatgtcagag gggtgccttg ggtaacctct 240 gggactcaga agtgaaaggg ggctattcctagttttattg ctatagccat tatgattatt 300 aatgatgagt attgattggt agtattggttatggttcatt gccggagaag tatattgttg 360 aagaggatag ctattagaag gattatggatgccgttgctt gcctgaagaa atacttgatg 420 gcagcttctg tggaaccaag gtttatttttttggntagaa ctggaataaa acctacatgt 480 ttatttctan gccactcagg taaaaaatcatgcnaactta acccttgata atgtgcctcc 540 aaaatgtaaa aaaataacgg ttggcccgggataatcccgt ncttggccga ccccctaggn 600 aattcccccc tg 612 507 632 DNA Homosapiens misc_feature (1)...(632) n = A,T,C or G 507 ggtactacgttgtagcccac ttccactatg tcctatcaat aggagctgta tttgccatca 60 taggaggcttcattcactga tttcccctat tctcaggcta caccctagac caaacctacg 120 ccaaaatccatttcactatc atattcatcg gcgtaaatct aactttcttc ccacaacact 180 ttctcggcctatccggaatg ccccgacgtt actcggacta ccccgatgca tacaccacat 240 gaaacatcctatcatctgta ggctcattca tttctctaac agcagtaata ttaataattt 300 tcatgatttgagaagccttc gcttcgaagc gaaaagtcct aatagtagaa gaaccctcca 360 taaacctggagtgactatat ggatgccccc caccctacca cacattcgaa gaacccgtat 420 acataaaatctagacaaaaa aggaaggaat cgaacccccc aaactgggtt nagccaaccc 480 catgggcttcacgacttttt tataaaaaaa aaaaaaaaaa aaaagtcctg gcccgggngg 540 cggtcanggngaaattcaac nactgggngg cggtctaang ggtccaactc gggnccaacc 600 tgggggaaaatgggaaagtg gttcctgggg aa 632 508 336 DNA Homo sapiens misc_feature(1)...(336) n = A,T,C or G 508 cggtcctcta atgctgctcn cccggccgcantgtgattgg atatcttgca gaattcgccc 60 ttagcgtggt cgccgggccg aggtacaacttccaaaaagg agacattgga gaanaaccaa 120 gctgggtcta taaggaattg cacatgagatggcacacata tttatgctgt ctgaaggnca 180 cgatcatgtt accatatcaa gctgaaaatgtcaccactat ctggagattt cgaccgtgtt 240 ttcctctctg aatctgttat gaacacnttggttggctgga ttcantaata aatatgtaag 300 gcctttcttt tcaaaaaaaa aaaaaaaaaaaaaagt 336 509 624 DNA Homo sapiens misc_feature (1)...(624) n = A,T,Cor G 509 ggtacttttt tttttttttt ttttttttta tagatacaat tggcttttatttgtgattca 60 tgagtcaggg cagtttccat tctgcaaaat atagtgatag ctcctactgggcaatacaac 120 agtanaacag tgggttttgt aaaatgggaa tccaggaaca gaagaatataaataaattga 180 tttaaataaa ctgattggtt aatttcagaa tacttcatat tacttttttctaagagttaa 240 agcagaaagg actttcttac tgtgctgact canacagcct ggactctcatgtttttagga 300 aaattttgct gttctgggat ctacctgctt cctcatgttt cagtgngagtatatggcatt 360 taacatgact ggctccattc tggagtccca ggctgtccct aaatgagaagttgactaaac 420 ataaggnatt aacactactg ncaggtacca tcattttggc ttncatcattcatanggtat 480 gatgncccnc naatcatacc tttatttgag tttttgncat tccnncccaaaaaaaaaatt 540 ttgaanttta ccaaaggntg catgccacnt ttaaagggtt anaaaatcnccccnccnggn 600 actaatnttg ggccatcngn nggc 624 510 619 DNA Homo sapiensmisc_feature (1)...(619) n = A,T,C or G 510 acggatgcta cttgtccaatgatggtaaaa gggtagctta ctggttgtcc tccgattcag 60 gttagaatga ggaggtctgcggctaggagt caataaagtg attggcttag tgggcgaaat 120 attatgcttt gttgtttggatatatggagg atggggatta ttgctaggat gaggatggat 180 agtaataggg caaggacgcctcctagtttg ttagggacgg atcggagaat tgtgtaggcc 240 aataggaaat atcattcgggcttgatgtgg ggaggggtgt ttaangggtt ggctagggta 300 taattgtctg ggtcccctaagaggtctggt gagaatagtg ttaatgtcat taangagaga 360 aagaaaaaaa ataagcccgagggcgtcttt gattgtgtan taaaggtgga angtgatttt 420 atcngaatgg gaagtgattnctaaggggtt ggtttgatcc ctttcgtgcc aaaataagaa 480 gnggaatgct gctagggctccataatgaag gcaanataaa atgaaagnaa aaaatctgta 540 aggnnggact gctactaatancctcccaaa tcttgaacaa gntttnccaa ttntggatgg 600 nggtataant tnaattcnn 619511 634 DNA Homo sapiens misc_feature (1)...(634) n = A,T,C or G 511cgaggacgcg gggagatggc ctagaagcaa tgatagccat cactgagaac acctagcacc 60caatcttggt tcctaatacc attctcccat caaaggaacc agagatcctt ggagaaatgg 120ttaaggaatg aggcaggaaa tatacaagat aagcctggag catcttatag ctctagaaag 180taagaaagta cctgcctatt ttagaatcct agagaacatt tcattgtaag aaactagccc 240attatttaag tgtccacagt atttttcatt tcagtggtcc aagatgcgaa ggtttccaga 300cacaatcttg ttctctaata ctgctccagg tgggatatca attctgtccc catgatttgc 360aatgatgata cccgttccct ttaatgaaac attttttnca aatgtcacat cttctgaaac 420tgngaggnga tccaattcaa gcatatctgg gntactttcc aaatcntctt agataatctt 480gaaccttcgt aaaagaactg gctaattaan ccanggccct gnaggaaatt ccccttttcc 540tcattggcag anancctgca ttaaantntt aagggttgnn ttnccnccan aaactgtgtg 600gtttgnaggc aaaaaacggt cttgggcatt ancc 634 512 623 DNA Homo sapiensmisc_feature (1)...(623) n = A,T,C or G 512 ggtacgcggg cattgttcatgactttaaca agaaacttac agcctattta gatcttaacc 60 tggataagtg ctatgtgatccctctgaaca cttccattgt tatgccaccc agaaacctac 120 tggagttact tattaacatcaaggctggaa cctatttgcc tcagtcctat ctgattcatg 180 agcacatggt tattactgatcgcattgaaa acattgatca cctgggtttc tttatttatc 240 gactgtgtca tgacaaggaaacttacaaac tgcaacgcag agaaactatt aaaggtattc 300 agaaacgtga agccagcaattgtttcgcaa ttcggcattt tgaaaacaaa tttgccgtgg 360 aaactttaat ttgtcttgaacagtcaagaa aaacattatt gaggaaaatt aatatcacag 420 catacccccc cctttacattttgngcagng gatatttttt aaagcttctt tnatgtaagt 480 agcaacangg ntttactatctttcatttca taaatcaatt aaancnttnc ctcaaaaaaa 540 aaaaaaaaaa aaaaatacctncccggcggc gctccaaagg ggaattcaan caccggnggc 600 cgtctttggg accaacncgggcc 623 513 623 DNA Homo sapiens misc_feature (1)...(623) n = A,T,C or G513 actgccctct ccagatcagc agttcaggag agcacaggag gcaaaacaca gattgctggg 60cttattggtg ccatcatcgt gctgattgtc gttctagcca ttggatttct cctggcacct 120ctacaaaagt ccgtcctggc agctttagca ttgggaaact taaagggaat gctgatgcag 180tttgctgaaa taggcagatt gtggcgaaag gacaaatatg attgtttaat ttggatcatg 240accttcatct tcaccattgt cctgggactc gggttaggcc tggcagctag tgtggcattt 300caactgctaa ccatcgtgtt caggacccaa tttccaaaat gcagcacgct ggctaatatt 360ggaagaacca acatctataa gaataaaaaa gattattatg atatgtatga gccagaagga 420gtgaaaattt cagatgtcca tcttctatct actttgcnaa cattggnttc tttaggcngg 480aacttatcga tgctggtngg ctttagtnca ctttgnaatt tacgcaagcc ccacaaactt 540tgaggaaatc ccaaactgcn aancangntt nttcagtggt acccaanggt tttttttcct 600tggcccgacn ccctangnga atn 623 514 627 DNA Homo sapiens misc_feature(1)...(627) n = A,T,C or G 514 ggtactcatg cccgactgtc taccaggcacacagactttg aggagagggc gtatgtcgtc 60 ttgatccgca tcaatgatgg gggtcggccacccttggaag gcattgtttc tttaccagtt 120 acattctgca gttgtgtgga aggaagttgtttccggccag caggtcacca gactgggata 180 cccactgtgg gcatggcagt tggtatactgctgaccaccc ttctggtgat tggtataatt 240 ttagcagttg tgtttatccg cataaagaaggataaaggca aagataatgt tgaaagtgct 300 caagcatctg aagtcaaacc tctgagaagctgaatttgaa aaggaatgtt tgaatttata 360 tagcaagtgc tatttcagca acaaccatctcatcctatta cttttcatct aacgtgcatt 420 ataatttttt aaacagatat tccctcttgtcctttaatat ttgctaaata tttctttttt 480 gangnggagt cttgctctgt cgnccaagctggantacctg ncccggccgg ccgtcaaagg 540 cgaattcaac aactggcggc cgtactaatggatcgacctc ggaccaactt ggggaacatg 600 gcanactngt tcctgngnaa aggatcc 627515 605 DNA Homo sapiens misc_feature (1)...(605) n = A,T,C or G 515accattggtg gccaattgat ttgatggtaa gggagggatc gttgacctcg tctgttatgt 60aaaggatgcc gtanggatgg gagggcgatg aggactagga tgatggcggg caggatagtt 120cagacggttt ctatttcctg agcgtctgag atgttagtat tagttaagtt ttgttgtgag 180tgttaggaaa agggcataca ggactaggaa gcagataagg aaaatgatta tgagggccgt 240gatcatgaaa ggtgataagc tcttctatga taggggaaag taancgtctt gtanacctac 300ttgcgctgca tgtgccatcc cgccgtaccc taacccgtgc aaaggtagca taatcacttg 360ttccttaatt aagggacctg tatgaatggc ttcaccaggg ttcaactgtc tcttactttt 420aaccagtgaa attgacctgc ccctgaanag gcggcnttac acaccagacg agaaaacctt 480tggagcttaa ttattatcca acatacctng ccggaccccc taaggcgaat tccaccactt 540gcggcgtcta tggatccact cggaccactt ggggaaaagg ctactgtcct ggnaatgttt 600cctcn 605 516 464 DNA Homo sapiens misc_feature (1)...(464) n = A,T,C orG 516 ggtacaacta atccgtgaca aattaccaga ttaattttac tttatttctt caggcctggg60 gtttttcgat gagttcaaat ttgggatctt caaatttgaa ggtgggaaat gtattcatgt 120ctgcattacc aaacatttgc ttgagcttaa aaagctccct ctccagctct tgctgatact 180ctgaactagc atcaacaggt cctccagatg tctgtcgctt agatttgtat tctctaatct 240tgtccacaaa gagtttctgt ataggatcaa gttccttatt aaatgccact gctgtaacac 300caatgttcct ccgcaaatgg actgagacgg ctgaccgaat gacagaggag aacctgaaga 360gcctctgaag aatcatgctg attcttgcac tcagtcccga gctgncaaag ccttcgccgc 420caccaccttc gntctacccc cgcgtacctg cccggcgggc gctc 464 517 611 DNA Homosapiens misc_feature (1)...(611) n = A,T,C or G 517 acccggagcacggagatctc gccggcttta cgttcacctc ggtgtctgca gcaccctccg 60 cttcctctcctaggcgacga gacccagtgg ctagaagttc accatgtcta ttctcaagat 120 ccatgccagggagatctttg actctcgcag gaatcccact gttgaggttg atctcttcac 180 ctcaaaaggtctcttcagag ctgctgtgcc cagtggtgct tcaactggta tctatgaggc 240 cctagagctccgggacaatg ataagactcg ctatatgggg aagggtgtct caaaggctgt 300 tgagcacatcaataaaacta ttgcgcctgc cctggttagc aagaaactga acgtcacaga 360 acaagagaagattgacaaac tgatgatcga gatggatgga acagaaaata aatctaagtt 420 tggtgcgaacgccattctgg gggtgtcctt tgccgtctgc naaactggtg ccgttgagaa 480 gggggtcccctgtccttggc cggacacnct aaggcgaatt ccacacactg cggccgtact 540 atggatcgactcggnaccaa cttgggtaat atgggcatac tggtnctggn gaaatgtttc 600 cctccaatcc a611 518 435 DNA Homo sapiens misc_feature (1)...(435) n = A,T,C or G 518cgaggtactt tntttttttt tttttttttt ttttaagagg aaaacccggt aatgatgtcg 60gggttgaggg ataggaggag aatgggggat aggtgtatga acatgagggt gttttctcgt 120gtgaatgagg gttttatgtt gttaatgtgg tgggtgagtg agccccattg tgttgtggta 180aatatgtaga gggagtatag ggctgtgact agtatgttga gtcctgtaag taggagagtg 240atatttgatc aggagaacgt ggttactagc acagagagtt ctcccagtag gttaatagtg 300gggggtaagg cgaggttagc gaggcttgct agaagtcatc aaaaagctat tagtgggagt 360agagtttgaa gtccttgaaa gaggattatg atgccactgt gaatgccttc ctagtttgag 420tttgctagcc cgcgt 435 519 407 DNA Homo sapiens misc_feature (1)...(407) n= A,T,C or G 519 actttntttt tttttttttt tttttttttt ncagctttgc aaccatactccccccggaac 60 ccaaagactt tggtttcccg gaagctgccc ggcgggtcat gggaataacgccgccgcatc 120 gccggtcggc atcgtttatg gtcggaacta cnacggtntn tgatcgtnttcnaacctccg 180 actttcgttc ttgattaatg aaaacattct tggcaaatgc tttcgctctggtccgtnttg 240 cgccggtcca anaatttcac ctctagcggc gcaatacnaa tgcccccggccgtccctctt 300 aatcatggcc tcagttccga aaaccaacaa aataaaaccg cggtcctattccattatgcc 360 tagctgcggt atccaggcgg tccccggtac ctnggccgng accacgc 407520 613 DNA Homo sapiens misc_feature (1)...(613) n = A,T,C or G 520accttctggg gcatacaaca tggcagcagg gcctcgggaa gaggggtagg aggaccgagc 60agcattctct gtagaggaag acaggaaagg agaccctctt ggcacacatt tatggagggt 120tgtccctgaa gagaagggca ggtgggagag gttccctgtt acttaagaga aggcaccagt 180ggcaaagagc acaatgaaga ggatgatgat aaaaacaatc acgcagataa ggacaatcat 240cttcacgttc ttccaccaga attttcgagc caccttctgc gatgtcgtct tgaagtgctc 300agatgtggct tccagatcct ctgtcttgtt gcggagatgt tccaagtttt ccccccgggc 360caggatccgc tccacattct gggtcataat attcttaact ccctccacct cactttgcag 420gttccgcaca cgatcatttc cttcaccttc actggcttnc tncatgtctc aaagcaccca 480gccggcagta agtgaatcgc ctatcggntt cttccaggng ggcctanttn anttctggtg 540gtcaactttc cccgcgtact tgggcggacc ccctaagggg aattcactgg cggccgtctt 600tggatccacc cgn 613 521 606 DNA Homo sapiens misc_feature (1)...(606) n =A,T,C or G 521 actgcagtaa aagctttaac aggtggaatt gcccacttat tcaaacagaataaggttgtt 60 catgtcaatg gatatggaaa gataactggc aaaaatcaag tcactgctacgaaagctgat 120 ggcggcactc aggttattga tacaaagaac attcttatag ccacgggttcagaagttact 180 ccttttcctg gaatcacgat agatgaagat acaatagtgt catctacaggtgctttatct 240 ttaaaaaaag ttccagaaaa gatggttgtt attggtgcag gagtaataggtgtagaattg 300 ggttcagttt ggcaaagact tggtgcagat gtgacagcag ttgaatttttangtcatgta 360 ggtggagttg gaattgatat ggagatatct aaaaactttc aacgcatccttcaaaaacag 420 gggtttaaat ttaaattgaa tacaanggta ctggtgctcc aagaagcanatggaaaaatt 480 gatgttctat tgaanctctt ttgngggaaa gctgaantnt acttggatgncctnggccgn 540 acncnctagg caatccncca ctggngccnt ntttggtccn cctggtccaactgggnnann 600 nggctn 606 522 617 DNA Homo sapiens misc_feature(1)...(617) n = A,T,C or G 522 acttgcgctt actttgtagc cttcatcagggtttgctgaa gatggcggta tataggctga 60 gcaagaggtg gtgaggttga tcggggtttatcgattacag aacaggctcc tctagaggga 120 tatgaagcac cgccaggtcc tttgagttttaagctgtggc tcgtagtgtt ctggcgagca 180 gttttgttga tttaactgtt gaggtttagggctaagcata gtggggtatc taatcccagt 240 ttgggtctta gctattgtgt gttcagatatgttaaagcca ctttcgtagt ctattttgtg 300 tcaactggag ttttttacaa ctcangtgagttttagcttt attggggagg gggtgatcta 360 aaacactctt tacgccggct tctattgacttgggttaatc gtgtgacccg cggtggctgg 420 cacgaaattg accaaccctg gggttagtataacttaatta aactttcntt attgctnaag 480 gtaatcctgg tggttnccct gggggngtngntaggctaaa cgtttgaacc tcattctgcg 540 gcctganctt ggccctttta tcgggggattaaaaggggac tncttgaacn gggngcttct 600 tggnaaatta taaaaca 617 523 608 DNAHomo sapiens misc_feature (1)...(608) n = A,T,C or G 523 cgaggtactttttttttttt tttttttttt ttttggaana agtaagcctt tatttccttg 60 ttttgcaaataaaactggct aagttggttg ctttttggtg attaagtcaa aganaccaaa 120 tcccatatcctcgtccgact cctccgactc ttccttggct tcaaccttan ctggggctgc 180 agcagcagcaggagcagctg tggtggtagc aaccacaggg gcagcancca caaaggcaga 240 tggatcaaccaanaaggcct tgaccttttc aacaagtggg aaggngtaat ccgtctccca 300 aacaaagtcaggactcgttt gtctcttcaa aaaaaaaaag cganggctcg catttggtcc 360 cctttggacattttgcaact cttcaatggg gttncattgg tnggtgatgg tataaacctt 420 tgangnacctgcccggccgg ccgtcaaang gcaaattcac ccactggcgg ccgttctatg 480 gatccnacccggncccaact tgggtaatat ggcanactgt tcctggggga aatgtntccc 540 tnaaattcccacaaanacaa nccgaaccta aangtaancn gggggccaag agggcnaccn 600 ccttattg 608524 398 DNA Homo sapiens 524 ggtacaggat cctctaaaga gaccgcctgg ctgggtgctcaaaccacatg ggccgaccca 60 aaagacgtca aaaccaagag ctgctcagga ggcactaaatgttgacggtc ttggccggct 120 tcacatcctc aatttcagca gacagccagc ggtaagtgcgatgacgccgc agcacctcaa 180 tggccttgag ttccagtggt gttgcctgaa taccaaggtcttctaagcca ggcaggtgag 240 gcaatttcat gtctgtgatg tgcatccgct ccactttatcccttgttatc cagggctcaa 300 atgggcttat ttcaaagact cttgctaccc atcgataggcaaaaagcggc aaggggaatg 360 ggaggaacaa tctgtgagcc acaacaaaga tgtacctg 398525 607 DNA Homo sapiens misc_feature (1)...(607) n = A,T,C or G 525actgttcctg ttggcccgag tggagactgg tgttctcaaa cccggtatgg tggtcacctt 60tgctccagtc aacgttacaa cggaagtaaa atctgtcgaa atgcaccatg aagctttgag 120tgaagctctt cctggggaca atgtgggctt caatgtcaag aatgtgtctg tcaaggatgt 180tcgtcgtggc aaccgttgct ggtgacagca aaaatgaccc accaatggaa gcagctggct 240tcactgctca ggtgattatc ctgaaccatc caggccaaat aagcgccggc tatgcccctg 300tattggattg ccacacggct cacattgcat gcaagtttgc tgagctgaag gaaaagattg 360atcgccgttc tggtaaaaag ctggaaaatg gccctaaatt cttgaaatct ggtgatgctg 420ccattggtga tatgggtcct ggcaagccca tgtgtgtttg agagcttctc aaactattca 480ccttgggtcc tttgctgtcg tgatatgaaa aaacagtgcg ggggtgtatc aaacatggac 540aaaagnttnt tgacttgcag gtaccaattt nccaaaacta aaaggtnaan aaatttncca 600aaccgcc 607 526 624 DNA Homo sapiens misc_feature (1)...(624) n = A,T,Cor G 526 cgaggtacgc gggggaagct ctgtttggtg ctttggatcc atttccatcggtccttacag 60 ccgctcgtca gactccagca gccaagatgg tgaagcagat cgagagcaagactgcttttc 120 aggaagcctt ggacgctgca ggtgataaac ttgtagtagt tgacttctcagccacgtggt 180 gtgggccttg caaaatgatc aagcctttct ttcattccct ctctgaaaagtattccaacg 240 tgatattcct tgaagtagat gtggatgact gtcaggatgt tgcttcagagtgtgaagtca 300 aatgcatgcc aacattccag ttttttaaga agggacaaaa ggtgggtgaattttctggag 360 ccaataagga aaagcttgaa gccaccatta atgaattagt ctaatcatgttttctgaaaa 420 tataaccagc ccattggcta tttaaaactt gtaatttttt taatttaccaaaatntaaaa 480 tntgaagacn taacccagtt gncatctgcg tgacaatnaa acattaatgctacactttta 540 aaaaaaaaaa aaaaaaaaaa gtcctgccng cggccctcaa aggggaattccacacctggg 600 ggccgtcttt nggncccacc cgnn 624 527 611 DNA Homo sapiensmisc_feature (1)...(611) n = A,T,C or G 527 acagagtgac actgaacagatcacaaagca cgagaaacat tagttctctc cctccccagc 60 gtctccttcg tctccctggttttccgatgt ccacagagtg agattgtccc taagtaactg 120 catgatcaga gtgctgtctttataagactc ttcattcagc gtatccaatt cagcaattgc 180 ttcatcaaat gccgtttttgccaggctaca ggccttttca ggagagttta gaatctcata 240 gtaaaagact gagaaatttagtgccagacc aagacgaatt gggtgtgtag gctgcatttc 300 tttcttacta atttcaaatgcttcctggta agcctgctgg gagttcgaca cagtggtttg 360 tttgttgctc cagatgccacttcagaaaga tcctaaaata atctcctttc attttcaagt 420 agaacacctt actttctggttgtgtagcat tgggaataaa atatttgtcc acagcttcag 480 aacatcattg cagatgtcctgcagtctggc tntatctttt acggnacctc ggccgggaca 540 ccctanggcg aattccacacctggcggccg tctantggac ngctnggcca cttgggnana 600 tggctactgt t 611 528 615DNA Homo sapiens misc_feature (1)...(615) n = A,T,C or G 528 ggtacttttttttttttttt ttttttttga gacggagtct tgttcagctg cccaggctgg 60 agtgcagtggctcgatcttc gctcactgca accaccgtct cctgggttca agcgattctc 120 ctgtctcagcctcccaagta gctgggatta caggccacca ccatcatgcc cggctaattt 180 ttgtatattggtagagacgg agtttcacta tgttgggcag gctggtcttg aactcctcac 240 ctcaggtgatccgcccgtct tggcctccca aagtgctagg attacaggcg taagccacca 300 tgcctggccagatgatgtat ttaaatatca taccaaactc tgtgtattta tataaagaaa 360 gactggtaaaagacttcctn attttaaaaa aaaccaaaac ccaaaccaaa aaaaacttta 420 cccttaccattgntgcatat tgtgcagtat aaaacacaca cttattngga catganaaaa 480 ccgnaagaaagncccgggta aactggactt tgccgccttt aaaaataaaa tcnaataagn 540 gccttgaggccctttttcaa tgcaattttt taacccggac ctgccnggng gcggtaaggg 600 naatccancnctggn 615 529 352 DNA Homo sapiens misc_feature (1)...(352) n = A,T,C orG 529 cgaggtactt tntttttttt tttttttttt tttttgggaa aagtcatgga ggccatgggg60 ttggcttgaa accagctttg gggggttcga ttccttcctt ttttgtctan attttatgta 120tacgggttct tcgaatgtgt ggtagggtgg ggggcatcca tatagtcact ccaggtttat 180ggagggttct tctactatta ggacttttcg cttcgaagcg aaggcttctc aaatcatgaa 240aattattaat attactgctg ttagagaaat gaatgagcct acagatgata ggatgtttca 300tgtggtgtat gcatcggggt agtccgagta acgtcggggc attcccccgc gt 352 530 769DNA Homo sapiens misc_feature (1)...(769) n = A,T,C or G 530 ggtactgcatagattaaaga aataaactgc agtaaagcca ctcgtaagga atgaacgcca 60 ttgccaatgataatcctctg cacataggtg gaaatagcaa agaagtatag ttgcttcaga 120 acaggtaataaccaaaatga taaacaccag aaataggaag ccaaacatgt aatacatctg 180 gtgtgaccaaatactattca gaatgaagaa aagttgtata aagatgcagc caaagggcaa 240 aatccctcccatgataatac caggcaaggg cttcgtgtag aacgactgtt caggaatctg 300 acngtggaatctgattggtt cgaactgggt gttcaatggc atcttcttaa aaccaangta 360 tgcaccaataaacgtcnnag gcacagatat gtanaccaaa gggccaatat ggcaancagt 420 gtncccaaaagaaatactgt tgganatcct ctncccagag gtcagattnt tattaagaat 480 cnccccgcgtcttttttttg tttttttttt gctccacttt nnggtaaann acntttnttt 540 aaaaatgtttaantctantt cctaattccc atnttctttn gctncnnnnc tgctggnggn 600 ctttaagggaantcnccnnt ggnggcgtcn atganccact tgnnactggn tantagcnac 660 gttcgggangttcccncntt ctaatatccg gnagtaannc ggctttgncn cctantggnn 720 cngcttttcgaacntgcctn anannntccg gaggtgtatn ttcttctnn 769 531 777 DNA Homo sapiensmisc_feature (1)...(777) n = A,T,C or G 531 cgaggtactt tttttttttttttttttttt tgtttttttt tttttttctt cagctaaaac 60 agcggaagag gtgatttattatatggttgt tacactcggc cacaaataaa cacagaaata 120 gtccanaatg tcacaggtccaggacagagg accaacatgg gcattttgtt tatgagcaag 180 gtgggtctna naggtgatcggcgatcagag ggcgatgaag ttctagatcc attgagacaa 240 gctctagaca gtagcatgcagtcccacaac ttgtctccaa agattcaggt ttactcacgt 300 catccagcan agaatggaaagtcaaatttc ctgaattgct atgtgtctgg gtttcatcca 360 tccgacattg aagttgacttactgaanaat ggagagagaa ttgaaaaant nggacattca 420 taactgnntt tcancaaggactggtctttc tatctcttgg ncttnntttt tcttntattt 480 tttttntaca tngggccttactttaaaaac atacntttcc nnnttacncn tggatgccaa 540 tngatttcna nanatttccnagnggaatcc tttngttatt nttaaaantt gggatctntn 600 gccancactt ggctaantnttaccnncttt nggaatngtc ntatgntcat tnttggaaat 660 tncccccctn angnntttctttnngngnta aaaattntta atnnttaaat tntttttcna 720 anattnntca aatactaanantnntnnggg nttanannaa tnntgtanat gggnnng 777 532 764 DNA Homo sapiensmisc_feature (1)...(764) n = A,T,C or G 532 actttacaag atagattgtataagaagcca aataatgaaa gcctagaaaa aactaattta 60 tacttatctg aaggttacaaattagacttt taaattttct ttgtagttgg tggtgtttga 120 gggttggcta gaaatgaaagcctggatttt gtgccatgtt tgtaatatag tttgttcctt 180 gatcaaataa tcagagaaaagaaacttaaa gatctttgtc tgtgaagaag aaaattatct 240 ccctagttca atctgtagtgaaataagact acagaaggca ttgttttttc ctttttattt 300 tntgnattat atatttttcttaaatatgtt ttattgtctt ctctaagcaa aaagttctta 360 ataaacatag tatttctctctgcgtcctat ttcattagtg aagacatagt tcacctaaaa 420 tggcatnctg ctctgaatctagctttttat aaatggctat gtttttgatg atatgtcaca 480 ttcaaaatgg cctaattaaatgtgttaaat gnaatggcac tcttataacc ttaaaataac 540 canaattaac cctccaaaaaaanaaaaaaa aaaaaggcct tggccgacnc ntangngant 600 caccnctgng gcntcatggacncttggcca cttgngaann nggtnangnt ccggganatt 660 tccccatncc aattcancggacatagnnac cnggccnaag ngnnccantg nngnnnnnct 720 tnnngaacng gccctnaacncccggggngg tngttcnccc tcnc 764 533 773 DNA Homo sapiens misc_feature(1)...(773) n = A,T,C or G 533 cgaggtactt tttttttttt ttttacagatacaattggct tttatttgtg attcatgagt 60 cagggcagtt tccattctgc aaaatatagtgatagctcct actgggcaat acaacagtag 120 aacagtgggt tttgtaaaat gggaatccaggaacagaaga atataaataa attgatttaa 180 ataaactgat tggttaattt cagaatacttcatattactt ttttctaaga gttaaagcag 240 aaaggacttt cttactgtgc tgactcagacagcctggact ctcatgtttt taggaaaatt 300 ttgtctgttc tgggatctac ctgcttcctcatgttcagtg tgagtatatg gcatttagca 360 tgactggtcc attctggagt caccaggcttgcacctaaat gagagttgac taancatagg 420 cnttaacact actgcagtac catcatttngacttcatcat catanggtat gatgncntct 480 aatnttncat tatttgagtt tggcattcagccacgagaga atattgcctt tgacaatgnt 540 gcatgcaact ttaaaggttt tagatncgccnccnggnact atttnngaaa tcgggggtcc 600 cccnanttgg agtttnacct ggcngaccnntgacnaccat taaggantgt tagantnccc 660 ttgaaccccc tttacaccnt ttgnatttcccggcntaacc ccgggcnnta agggatccnt 720 tggcntnngg cccngcnatn gaagnacnttngannacgcc tccncaccan nng 773 534 730 DNA Homo sapiens misc_feature(1)...(730) n = A,T,C or G 534 acacagacaa atttatgcga ccagggcagaggctgtagat gattcatatt tccaattggg 60 agggaggact cgcttggtct tataatatcgagccaaacgg tgaatccggc tctctattag 120 aatcagacgg aatttagcat ccttatcctttctgttcctc tcaagatgct ttcgaacagc 180 aactgctttc ttaattaaat ggtagagatcttcaggaaga tcaggagcaa gtcccttaga 240 cttaagaatt cttaaaattt tattgcctgtcacaaaacgt acaaattgac caggctgttg 300 acggctgcct ccacgtcggt ggaataattctgacgaatct gggagctcat ggttggttgg 360 caagaaggag ctaccacaaa aacngtgctgcaggtccaga agcaggagat ggccgaaaaa 420 tgtcccgaag ttcaaccgag aggaaatcgaggcggccgag cttgaagaag tcccgattgt 480 tcgtcaacct gtgaacagaa caaccccggaccgcnantgc ccggtnctgg ccggacacct 540 angggaatcn accctgnggc gtctangaccacttggccaa ctggganntg gaaatntccg 600 ggaaagntcn tcaatcccaa ttaccgacnaagaactgggc naagggtcnc atatgggcnc 660 gccttnnnga nctnccctta annccccggagggtgntggn tctcntctan nntnnngtgg 720 nggnnaanag 730 535 809 DNA Homosapiens misc_feature (1)...(809) n = A,T,C or G 535 gcgtggtcngcggccgaggt accaactgca gagccaggaa aactttgaag ccttcatgaa 60 ggcaatcggtctgccggaag agctcatcca gaaggggaag gatgtcaagg gggtgtcgga 120 aatcgtgcagaatgggaagc acttcaagtt caccatcacc gctgggtcca aagtgatcca 180 aaacgaattcacggtggggg aggaatgtga gctggagaca atgacagggg ataaagtcaa 240 gacagtggttcanttggaag gtgacaataa actggtgaca actttcaaaa acatcaagtc 300 tgtgaccgaactcaacggng acataatcac caataccatg acattgggtg acattgtctt 360 caagagaatcagcangagaa tttaaacaag tctgcatttc atattatttt antgntgtaa 420 aattaatgtaattaaagtga actttgttta aaaaaagann nntnntntaa atanaaaaaa 480 gtncctgcctggcggccggt caaaggccaa ttccagcnac tngnggccnt actagtgatc 540 nactcgtcnaacttgcgtaa nntggcatac ttgtnctngg taaatntatc cctcncatcn 600 ccaaattcnnccgagcttaa atntaaactg gggcctatag gnncactcct tttggttgcn 660 ctgccnttnnacgaacttcg ncccttttat antgcccccc ganagggtng tttggctttc 720 ntnntatattctctctctcc ttgnnggttt ttanggtngg tcatntgggn tctntanttt 780 agcttngaanntantngntn tttnttnnt 809 536 755 DNA Homo sapiens misc_feature(1)...(755) n = A,T,C or G 536 actttttttt tttttttttt ttttttttttatgaggaaaa cccggtaatg atgtcggggt 60 tgagggatag gaggagaatg ggggataggtgtatgaacat gagggtgttt tctcgtgtga 120 atgagggttt tatgttgtta atgtggtgggtgagtgagcc ccattgtgtt gtggtaaata 180 tgtagaggga gtatagggct gtgactagtatgttgagtcc tgtaagtagg agagtgatat 240 ttgatcagga gaacgtggtt actagcacagagagttctcc cagtaggtta atagtggggg 300 gtaaggcgag gttagcgagg cttgctagaagtcatcaaaa agctattant gggagtanag 360 tttgaagtcc ttgagagagg attatgatgcnacttgtaat gcnttcgant ttgagtttgc 420 tagcngaata nnatgaggat gtantccngggccaatatna aaatactccc cgtnaacttn 480 aggggttnga taaaatgctg tctacccnngactttgccgn acaccttagg caattcanca 540 ctggngccgt ctnanggncc cacttggnccacnttggnga acatggcnnc ngtcntngga 600 aatgtttcnt caattcccnc ttcnaccgantantgnaacn ggggcanaag cncccatatn 660 gtccctccct tctngaactt nnccnttaaatncccccgga gggttnatgg ctttctctnc 720 taananntnt tnngnggnnt tcnataannataann 755 537 794 DNA Homo sapiens misc_feature (1)...(794) n = A,T,C orG 537 cgaggtacga aagggacaag agaaataagg cctacttcac aaagcgcctt cccccgtaaa60 tgatatcatc tcaacttagt attataccca cacccaccca agaacagggt ttgttaaaaa 120aaaaaaaaaa aaaaaaaaaa aaaaaagtac cttgactttg ttcacagcat gtagggtgat 180gagcactcac aattgttgac taaaatgctg cttttaaaac ataggaaagt agaatggttg 240agtgcaaatc catagcacaa gataaattga gctagttaag gcaaatcagg taaaatagtc 300atgattctat gtaatgtaaa ccagaaaaaa taaatgttca tgatttcaag atgttatatt 360aaagaaaaac tttaaaaatt attatatatt tatagcaaaa gttatcttaa atatgaattc 420tgttgtaatt taatgctttt gaatacagag atntaaatga agtattatct gtaaaaatgt 480atattagagt tgtgatacag agtatatttc attcanccat nttcatacta ataatatgga 540tttaaanata tcctataaat tcnaattcaa nanaaannnt gntananaan aanggnctgn 600cggcggcgca nggcaattca acaatgnggc gtctanggac nactggtcca cttgggaana 660ggcaacttnc tgggaatgat ccttcattcc canntaccna gctanttaac nggggcaaag 720ggcccnntta tgggnntngc ntntnnaant tgcccttaaa accccggngg gtgntggntc 780tttnnntttn ngnt 794 538 766 DNA Homo sapiens misc_feature (1)...(766) n= A,T,C or G 538 ggtacgcggg ggaaggcctt cctttttcgt ctgggctgcc aacatgccatccagactgag 60 gaagacccgg aaacttaggg gccacgtgag ccacggccac ggccgcataggcaagcaccg 120 gaagcacccc ggcggccgcg gtaatgctgg tggtctgcat caccaccggatcaacttcga 180 caaataccac ccaggctact ttgggaaagt tggtatgaag cattaccacttaaagaggaa 240 ccagagcttc tgcccaactg tcaaccttga caaattgtgg actttggtcagtgaacagac 300 acgggtgaat gctgctaaaa acaagactgg ggctgtccca tcattgatgtggtgcgatcg 360 gctactacaa agttctggga aagggaaagc tccaaagcaa nctgtcatcgtgaaggccaa 420 atcttcacag aagagctgag gagaaaaata agantgttgg ggggcctgtgtctggtgctt 480 gaagcccatt ganggagttt aattaatgct actcttttga aaaaaanannaananaaaaa 540 gacctgcccg gcggcngtaa ggcaattcac cnttgngccg tctaaggaccactggccaan 600 tgggaanang gcnaanntcc tgggaatngt tcntcaattc cccaattaaccaanaangna 660 acnngggcca nnnggcaccc ttatggntcc ctncctttng gaactngccttttaatccnc 720 cngagggtnt tgctccttnt ntttntgnnt ggggtaatna aaagtn 766539 789 DNA Homo sapiens misc_feature (1)...(789) n = A,T,C or G 539accattggtg gccaattgat ttgatggtaa gggagggatc gttgacctcg tctgttatgt 60aaaggatgcg tagggatggg agggcgatga ggactaggat gatggcgggc aggatagttc 120agacggtttc tatttcctga gcgtctgaga tgttagtatt agttagtttt gttgtgagtg 180ttaggaaaag gcatacagga ctaggaagca gataaggaaa atgattatga gggcgtgatc 240atgaaaggtg ataagctctt ctatgatagg ggaagtagcg tcttgtagac ctacttgcgc 300tgcatgtgcc cccgcgtact tgactttctt ttntatttnt tttattnttt ttgactactt 360agaattttca caattctaat aagattgttc caagtctctc atgtgcaagc tttaaaggat 420gactcttgcc atttatgtac ctcggncgcg accacgctaa gggcaaattc agcacacttg 480cggncgttct aagtggatcc nagctcggtc caaccttgcg tatcatggca tactggtccc 540tngtgaaatg tatcccttac aatcncacac atcnancccg aanctaaann taaanctggg 600gccaataata ctactncata atgctcnctn ctgccnttca ncnggaacnt gtgcncttnt 660tatnatggca acncggaagn gtggttggcc ttcctctcta aaacntgnng gntngttgga 720aggganctct aggnnncggt ccaattggan ncgaaattnt agctntntac naaanatntt 780tttttcncg 789 540 747 DNA Homo sapiens misc_feature (1)...(747) n =A,T,C or G 540 acttttaagg gcataataag ggttaacatt ctaggcagta taaacacaccccataatgca 60 agtaataggt aatctagaga tgtggacttt attgctatat gggaattacatttaaatttg 120 agggcatttt atataaagaa aaatacagac ctataaagtt tggcatattcattaagttat 180 cttttaatat ttttttctag aaaacaggtg acatttgtat ctacgataaaaatttttata 240 cagaacctac tgcctcaaac tgaatcccat caagaaaact agtttctattgnattaagta 300 actcaaaata aattatcact tcgaaaactt gctttccaca ctaaggtaagtcagactaga 360 tgaacactcc agaattttta ctacagactg ttttaagtta gaagtgatggcaatttataa 420 attgagaata tcctccctga tgccctaact ggccaaacca aaatctaagaaagcagtgac 480 ncctcttact atnatgaact tctgaatang gtagggacct cctggcntannnatgaaaan 540 ncctggccga ccccctaggg aatccncact gggggcctnn anggaccnantggccaantt 600 gnnanngggn aangnncctg gnaatgtccn caattcncna atnccgncnaaagtaacngg 660 gcccnggggn annnnnangn ngncnnccnn nnngaannng cccttnaannncccgngggg 720 ggngggntct nnncnnnncc nnngggg 747 541 773 DNA Homosapiens misc_feature (1)...(773) n = A,T,C or G 541 cgaggtaccatgaaatacat atatttcata aggttcagtt acaaaatgga ttgtttcaaa 60 tggcaatttcttacactaac ctgattatga aaaaaagaag tctgtatcat ctgcttccaa 120 gtctgttatgtccaaatata ttttaattat gcatttattt tgctactttt ataaatatta 180 gagatttcaccttaaattat ttttgtaact agttctagaa catgttttcc aattattatt 240 tttctaatggagacatataa ttgacctatg tttatgcata tatgttctct acacagtgaa 300 actttttttaaaaagaatag taaagaaaat gcggaagctc tggctctcca aggcaaagtc 360 aaaaaaaaaaaaaaagcggg ggggaatgcg aggaacattt tattacacct cctgatttca 420 ctccttgagtttattttctc ccttggttat tggttaatgc tagaaactgn attctaagag 480 agcatccttttcaggtgacn tgataattgg aagatttgat ccttccgcga cctgnccggc 540 ggccgtcnaanggcnattcc anccactggc ggcggtctaa nggatcnact tggnccacct 600 ggctaactggcaacnggtcc ngggngaaat gnatccttaa atccncactc nacccgacct 660 aangaactggggcaagggnc accctatggn gctcngcctt cnngaantnn ccnncttaan 720 aaccnggggngntggnntct nnnnnannnn cnnnntgngg gnntaanaag ann 773 542 770 DNA Homosapiens misc_feature (1)...(770) n = A,T,C or G 542 cgaggtactttttttttttt tttttttttt ttttttttag aattctgaat tttattagag 60 aatatatctaaaatacaata tttattaagt tatgatatat tgnctgaatg gaaatatact 120 ctgnatcgcaactctaatta taacaatttt tacagataat acttcattta tatctctgna 180 attcaaaagtcattaaatta caacagaatt catatttaag ataactttgc tataaatata 240 taataatttttaaagttttt ctttaatata acatcttgaa atcatgaaca tttatttttt 300 ctgggttacattcatagaat catgactatt ttacctgatt tgccttaact agctcaattt 360 atcttggctatggatttgca ctcaccattc tactttccta tgtttaaaag cacattttag 420 tcacaattgngagtgctcat caccctacat gctgtgacaa aggcaagggc ctgcccgggc 480 ggccgtncaaanggcgaatt ccncaactgg cggcggtcca agtggancga ctcggaccaa 540 ctngggaacatggcaactgg tcccggggaa atggaaccgt acattcccca natcagccga 600 ncttaggtaaacngggggcn aagggggcta cncataatgg nggtccnccc ttcatngaac 660 cgngccctnntatnatgcac cccggagggt nnttngcctc ctcntnnnnn ntcngntgtg 720 gagggagtccnggggggtnc cangggggna aaaantgccn ngncccggng 770 543 748 DNA Homo sapiensmisc_feature (1)...(748) n = A,T,C or G 543 accgcgggat gcccctcatttacataaata ttatactagc atttaccatc tcacttctag 60 gaatactagt atatcgctcacacctcatat cctccctact atgcctagaa ggaataatac 120 tatcgctgtt cattatagctactctcataa ccctcaacac ccactccctc ttagccaata 180 ttgtgcctat tgccatactagtctttgccg ctgcgaagca gcggtgggcc tagccctact 240 agtctcaatc tccaacacatatggcctaga ctacgtacat atgctaggcc atatggtaac 300 tctatgttta acattttgaggaactgccaa actgttttcc aaagtgacta cactatttta 360 cattcccacc ttgaaggtccaatttctcga cattctacca acatgggtaa tggctgcttt 420 ttatttagca accttaatgggtgtgaagag atactcaatg ggaatttgat tgattcccta 480 angctaatga tttggnttctttctggctga ngccagagnt atctntttgg gaaaattatt 540 naancttgnc atttaacnngcngatttatn tgatntanaa tnttntattt gganccngcc 600 tttaagnaag nttaaaattnncaatnttgg ggcttncttt tggccatgan naannttaat 660 nntannanna attnnntncnannnggcnng tnaannannn nnnanaaana annnttnnna 720 anaannactt tttnnnnnnacntggcgg 748 544 327 DNA Homo sapiens misc_feature (1)...(327) n = A,T,Cor G 544 actttttttt tttttttttt tttttttttt tttttttttt ttggctctagagggggtaga 60 gggggtgcta tagggtaaat acgggcccta tttcaaagat ttttaggggaattaattctg 120 ggacgatggg catgaaactg tggtttgctc cacagatttc anagcattgaccgtagtata 180 cccccggtcg tgtagcggtg aaagtggttt ggtttaaacg tccgggaattgcatctgttt 240 ttaagcctaa tgtggggaca gctcatgagt gcaagacgtc ttgtgatgtaattattatac 300 gaatgggggc ttnaatcggg agtacct 327

We claim:
 1. An isolated nucleic acid comprising a nucleotide sequencewhich hybridizes under stringent conditions to a sequence of SEQ ID Nos.1-35 or a sequence complementary thereto.
 2. An isolated nucleic acidcomprising a nucleotide sequence at least 80% identical to a sequencecorresponding to at least about 15 consecutive nucleotides of one of SEQID Nos. 1-35 or a sequence complementary thereto.
 3. An isolated nucleicacid comprising a nucleotide sequence of SEQ ID Nos. 1-35 or a sequencecomplementary thereto.
 4. A nucleic acid according to claim 1, furthercomprising a transcriptional regulatory sequence operably linked to saidnucleotide sequence so as to render said nucleotide sequence suitablefor use as an expression vector.
 5. An expression vector, capable ofreplicating in at least one of a prokaryotic cell and eukaryotic cell,comprising the nucleic acid of claim
 4. 6. A host cell transfected withthe expression vector of claim
 5. 7. A transgenic animal having atransgene of the nucleic acid of claim 1 incorporated in cells thereof,which transgene modifies the level of expression of the nucleic acid,the stability of an mRNA transcript of the nucleic acid, or the activityof the encoded product of the nucleic acid.
 8. A substantially purenucleic acid which hybridizes under stringent conditions to a nucleicacid probe corresponding to at least 12 consecutive nucleotides of oneof SEQ ID Nos. 1-168 or a sequence complementary thereto.
 9. Apolypeptide including an amino acid sequence encoded by a nucleic acidof claim 1 or a fragment comprising at least 25 amino acids thereof. 10.A probe/primer comprising a substantially purified oligonucleotide, saidoligonucleotide containing a region of nucleotide sequence whichhybridizes under stringent conditions to at least 12 consecutivenucleotides of sense or antisense sequence selected from SEQ ID Nos.1-168.
 11. An array including at least 10 different probes of claim 10attached to a solid support.
 12. The probe/primer of claim 10, furthercomprising a label group attached thereto and able to be detected. 13.The probe/primer of claim 12, wherein said label group being selectedfrom radioisotopes, fluorescent compounds, enzymes, and enzymeco-factors.
 14. An antibody immunoreactive with a polypeptide of claim9.
 15. An antisense oligonucleotide analog which hybridizes understringent conditions to at least 12 consecutive nucleotides of one ofSEQ ID Nos. 1-35 or a sequence complementary thereto, and which isresistant to cleavage by a nuclease.
 16. A test kit for determining thephenotype of transformed cells, comprising the probe/primer of claim 12,for measuring a level of a nucleic acid which hybridizes under stringentconditions to a nucleic acid of SEQ ID Nos. 1-544 in a sample of cellsisolated from a patient.
 17. A test kit for determining the phenotype oftransformed cells, comprising an antibody specific for a protein encodedby a nucleic acid which hybridizes under stringent conditions to any oneof SEQ Nos. 1-544.
 18. A method of determining the phenotype of a cell,comprising detecting the differential expression, relative to a normalcell, of at least one nucleic acid which hybridizes under stringentconditions to one of SEQ ID Nos. 1-544, wherein the nucleic acid isdifferentially expressed by at least a factor of two.
 19. A method fordetermining the phenotype of cells in a sample of cells from a patient,comprising: i. providing a nucleic acid probe comprising a nucleotidesequence having at least 12 consecutive nucleotides of any of SEQ IDNos. 1-544; ii. obtaining a sample of cells from a patient; iii.providing a second sample of cells substantially all of which arenon-cancerous; iv. contacting the nucleic acid probe under stringentconditions with mRNA of each of said first and second cell samples; andv. comparing (a) the amount of hybridization of the probe with mRNA ofthe first cell sample, with (b) the amount of hybridization of the probewith mRNA of the second cell sample, wherein a difference of at least afactor of two in the amount of hybridization with the mRNA of the firstcell sample as compared to the amount of hybridization with the mRNA ofthe second cell sample is indicative of the phenotype of cells in thefirst cell sample.
 20. A method of determining the phenotype of a cell,comprising detecting the differential expression, relative to a normalcell, of at least one protein encoded by a nucleic acid which hybridizesunder stringent conditions to one of SEQ ID Nos. 1-544, wherein theprotein is differentially expressed by at least a factor of two.
 21. Themethod of claim 20, wherein the level of said protein is detected in animmunoassay.
 22. A method for determining the presence or absence of anucleic acid which hybridizes under stringent conditions to one of SEQID Nos. 1-168 in a cell, comprising contacting the cell with a probe ofclaim
 10. 23. A method for determining the presence of absence of apolypeptide encoded by a nucleic acid which hybridizes under stringentconditions to one of SEQ ID Nos. 1-35 in a cell, comprising contactingthe cell with an antibody of claim
 14. 24. A method for detecting amutation in a test nucleic acid which hybridizes under stringentconditions to a nucleic acid of SEQ ID Nos. 1-544 or a sequencecomplementary thereto, comprising i. collecting a sample of cells from apatient, ii. isolating nucleic acid from the cells of the sample, iii.contacting the nucleic acid sample with one or more primers whichspecifically hybridize to a nucleic acid sequence of SEQ ID Nos. 1-544under conditions such that hybridization and amplification of thenucleic acid occurs, and iv. comparing the presence, absence, or size ofan amplification product to the amplification product of a normal cell.25. A method for identifying an agent which alters the level ofexpression in a cell of a nucleic acid which hybridizes under stringentconditions to one of SEQ ID Nos. 1-544 or a sequence complementarythereto, comprising i. providing a cell; ii. treating the cell with atest agent; iii. determining the level of expression in the cell of anucleic acid which hybridizes under stringent conditions to one of SEQID Nos. 1-544 or a sequence complementary thereto; and iv. comparing thelevel of expression of the nucleic acid in the treated cell with thelevel of expression of the nucleic acid in an untreated cell, wherein achange in the level of expression of the nucleic acid in the treatedcell relative to the level of expression of the nucleic acid in theuntreated cell is indicative of an agent which alters the level ofexpression of the nucleic acid in a cell.
 26. A pharmaceuticalcomposition comprising an agent identified by the method of claim 25.27. A pharmaceutical composition comprising a nucleic acid whichincludes a nucleotide sequence which hybridizes under stringentconditions to one of SEQ ID Nos. 1-544 or a sequence complementarythereto.
 28. A pharmaceutical composition comprising a polypeptideencoded by a nucleic acid which includes a nucleotide sequence thathybridizes under stringent conditions to one of SEQ ID Nos. 1-544 or asequence complementary thereto.
 29. An isolated nucleic acid comprisinga portion of a nucleotide sequence of SEQ ID Nos. 36-168 or a sequencecomplementary thereto.
 30. A gene which hybridizes to one of SEQ ID Nos.1-35.
 31. A method for detecting cancer in which one or more of SEQ IDNos. 1-544 are used as probes, said method comprising: i. collecting asample of cells from a patient, ii. isolating nucleic acid from thecells of the sample, iii. contacting the nucleic acid sample with one ormore primers which specifically hybridize to a nucleic acid sequence ofSEQ ID Nos. 1-544 under conditions such that hybridization andamplification of the nucleic acid occurs, and iv. comparing thepresence, absence, or size of an amplification product to theamplification product of a normal cell.
 32. A method of claim 31 inwhich said cancer is colon cancer.
 33. A method for detecting cancer ina patient sample in which an antibody to a protein encoded by SEQ IDNos. 1-544 is used to react with proteins in said sample.
 34. A methodof claim 33 in which said cancer is colon cancer.